New tools to study RNA interference to fish viruses: Fish cell lines permanently expressing siRNAs targeting the viral polymerase of viral hemorrhagic septicemia virus

Antiviral Research

Volumn 82, Issue 3, 2009, Pages 148-156

  Ruiz, S ^a   Schyth, B D ^c   Encinas, P ^a   Tafalla, C ^a   Estepa, A ^b   Lorenzen, N ^c   Coll, J M ^a  

^a INIA   (Spain)

^b MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

EPC; Fish cell lines; Hairpin; Polymerase; siRNA; VHSV

Indexed keywords

DNA; DNA DIRECTED DNA POLYMERASE ALPHA; SMALL INTERFERING RNA;

ANTIVIRAL ACTIVITY; ARTICLE; CARP; CELL TRANSFORMATION; CONTROLLED STUDY; DRUG SCREENING; DRUG TARGETING; GENE EXPRESSION; GENE TARGETING; GENETIC TRANSFECTION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; RNA INTERFERENCE; RNA SEQUENCE; VIRAL HEMORRHAGIC SEPTICEMIA VIRUS; VIRUS INFECTIVITY;

ANIMALS; CARPS; CELL LINE; NOVIRHABDOVIRUS; RNA INTERFERENCE; RNA REPLICASE; RNA, SMALL INTERFERING;

CYPRINIDAE; EPITHELIOMA PAPULOSUM; RHABDOVIRIDAE; VIRAL HEMORRHAGIC SEPTICEMIA VIRUS;

EID: 64749096678     PISSN: 01663542     EISSN: 18729096     Source Type: Journal    
DOI: 10.1016/j.antiviral.2009.02.200     Document Type: Article

References (26)

1. Baltayiannis G., Filis S., Tsianos E., and Karayiannis P. Small interfering RNA(siRNA)-mediated inhibition of hepatitis C virus replication using bicistronic vector in vivo (cell line Huh-7) and in vitro. J. Hepatol. 42 (2005) 154
2. Barik S. Control of nonsegmented negative-strand RNA virus replication by siRNA. Virus Res. 102 (2004) 27-35
3. Basurco B., Sanz F., Marcotegui M.A., and Coll J.M. The free nucleocapsids of the viral haemorrhagic septicaemia virus contain two antigenically related nucleoproteins. Arch. Virol. 119 (1991) 153-163
4. Caplen N.J. RNAi as a gene therapy approach. Expert Opin. Biol. Ther. 3 (2003) 575-586
5. Colbère-Garapin F., Blondel B., Saulnier A., Pelletier I., and Labadie K. Silencing viruses by RNA interference. Microbes Infect. 7 (2005) 767-775
6. Coll J.M. The glycoprotein G of rhabdoviruses. Arch. Virol. 140 (1995) 827-851
7. Gruber J., Manninga H., Tuschl T., Osborn M., and Weber K. Specific RNAi mediated gene knockdown in zebrafish cell lines. RNA Biol. 2 (2005) 101-105
8. Heike S., Egbert M., and Mettenleiter T.C. Complete genomic sequence of viral hemorrhagic septicemia virus, a fish rhabdovirus. Virus Genes 19 (1999) 59-65
9. Korf M., Jarczak D., Beger C., Manns M.P., and Krüger M. Inhibition of hepatitis C virus translation and subgenomic replication by siRNAs directed against highly conserved HCV sequence and cellular HCV cofactors. J. Hepatol. 43 (2005) 225-234
10. Kurath G., Higman K.H., and Björklund H.V. The NV genes of fish rhabdoviruses: development of RNase protection assays for rapid assessment of genetic variation. Vet. Res. 26 (1995) 477-485
11. LaPatra S.E., Lauda K.A., and Jones G.R. Antigenic variants of infectious hematopoietic necrosis virus and implications for vaccine development. Dis. Aquat. Organisms 20 (1994) 119-126
12. LeBerre M., De Kinkelin P., and Metzger A. Identification sérologique des rhabdovirus des salmonidés. Bull. Office Int. Epizooties 87 (1977) 391-393
13. Li T., Zhang Y., Fu L., Yu C., Li X., Li Y., Zhang X., Rong Z., Wang Y., Ning H., Liang R., Chen W., Babiuk L.A., and Chang Z. siRNA targeting the leader sequence of SARS-CoV inhibits virus replication. Gene Ther. 12 (2005) 751-761
14. Lorenzen N., and LaPatra S.E. DNA vaccines for aquacultured fish. Rev. Sci. Technol. 24 (2005) 201-213
15. Lorenzo G., Estepa A., and Coll J.M. Fast neutralization/immunoperoxidase assay for viral haemorrhagic septicemia with anti-nucleoprotein monoclonal antibody. J. Virol. Methods 58 (1996) 1-6
16. Ma H., Shied K.J., Chen G., Qiao X.T., and Chuang M.Y. Application of real time polymerase chain reaction (RT-PCR). J. Am. Sci. 2 (2006) 1-5
17. Novina C.D., Murray M.F., Dykxhoorn D.M., Beresford P.J., Riess J., Lee S.K., Collman R.G., Lieberman J., Shankar P., and Sharp P.A. siRNA-directed inhibition of HIV-1 infection. Nat. Med. 8 (2002) 681-686
18. Purcell M.K., Hart S.A., Kurath G., and Winton J.R. Strand-specific, real-time RT-PCR assays for quantification of genomic and positive-sense RNAs of the fish rhabdovirus, infectious hematopoietic necrosis virus. J. Virol. Methods 132 (2006) 18-24
19. Rocha A., Ruiz S., and Coll J.M. Improvement of transfection efficiency of epithelioma papulosum cyprini carp cells by modification of their cell cycle and use of an optimal promoter. Mar. Biotechnol. 6 (2004) 401-410
20. Sanz F., and Coll J.M. Detection of viral haemorrhagic septicemia virus by direct immunoperoxidase with selected anti-nucleoprotein monoclonal antibody. Bull. Eur. Assoc. Fish Pathol. 12 (1992) 116-119
21. Sanz F.A., and Coll J.M. Detection of hemorrhagic septicemia virus of salmonid fishes by use of an enzyme-linked immunosorbent assay containing high sodium chloride concentration and two noncompetitive monoclonal antibodies against early viral nucleoproteins. Am. J. Vet. Res. 53 (1992) 897-903
22. Schyth B.D., Lorenzen N., and Pedersen F.S. Antiviral activity of small interfering RNAs: specificity testing using heterologous virus reveals interferon-related effects overlooked by conventional mismatch controls. Virology 349 (2006) 134-141
23. Schyth B.D., Lorenzen N., and Pedersen F.S. A high throughput in vivo model for testing delivery and antiviral effects of siRNAs in vertebrates. Mol. Ther. 15 (2007) 1366-1372
24. Wagner R.R. The Rhabdoviruses (1987), Plenum Press, NY 543
25. Xia X.G., Zhou H., Ding H., Affar E.B., Shi Y., and Xu Z. An enhanced U6 promoter for synthesis of short hairpin RNA. Nucleic Acids Res. 31 (2003) e100
26. Zenke K., and Kim K.H. Novel fugu U6 promoter driven shRNA expression vector for efficient vector based RNAi in fish cell lines. Biochem. Biophys. Res. Commun. 371 (2008) 480-483