Fast neutralization/immunoperoxidase assay for viral haemorrhagic septicaemia with anti-nucleoprotein monoclonal antibody

Journal of Virological Methods

Volumn 58, Issue 1-2, 1996, Pages 1-6

  Lorenzo, G ^a   Estepa, A ^a   Coll, J M ^a  

^a Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria   (Spain)

Author keywords

Enzyme immunohistochemical procedure; Trout antibodies; Viral haemorrhagic septicaemia virus

Indexed keywords

MONOCLONAL ANTIBODY; VIRUS NUCLEOPROTEIN;

ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOPEROXIDASE STAINING; NONHUMAN; PRIORITY JOURNAL; TROUT; VIRUS DETECTION; VIRUS NEUTRALIZATION;

BARBUS BARBUS; CYPRINUS CARPIO; SALMONIDAE; VIRAL HEMORRHAGIC SEPTICEMIA VIRUS;

EID: 0029894714     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-0934(95)01972-3     Document Type: Article

References (30)

1. Basurco, B. and Coll, J.M. (1989) Spanish isolates and reference strains of viral haemorrhagic septicaemia virus shown similar protein size patterns. Bull. Eur. Assoc. Fish Pathol. 9, 92-95.
2. Basurco, B. and Coll, J.M. (1992) In vitro and in vivo immunization with the first viral haemorrhagic septicaemia viruses isolated in Spain compared to international reference serotypes. Res. Vet. Sci. 53, 93-97.
3. Bernard, J., Bremont, M. and Winton, J. (1991) Sequence homologies between the N genes of the 07.71 and Makah isolates of viral, haemorrhagic septicaemia virus. Proceedings of the II International Symposium on Viruses of Lower Vertebrates. Oregon State University, pp. 109-116.
4. Bernard, J., Bremont, M. and Winton, J. (1992) Nucleocapsid gene sequence of a North American isolate of viral haemorrhagic septicaemia virus, a fish rhabdovirus. J. Gen. Virol. 73, 1011-1014.
5. Bernard, J. and DeKinkelin, P. (1985) Effect of UV irradiation of Viral Haemorrhagic Septicemia Virus on virus-specific intracellular synthesis. Ann. Inst. Pasteur/Virology 136, 213-222.
6. Bernard, J., Lecocq-Xhonneux, F., Rossius, M., Thiry, M.E. and De Kinkelin, P. (1990) Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagic septicaemia virus. J. Gen. Virol. 71, 1669-1674.
7. Coll, J.M. (1989) Injection of physiological saline facilitates recovery of ascitic fluids for antibody production. J. Immunol. Methods 104, 219-222.
8. Coll, J.M. (1995a) The glycoprotein of rhabdoviruses. Arch. Virol. 140, 827-851.
9. Coll, J.M. and Dominguez, J. (1994) Applications of monoclonal antibodies in Aquaculture. Biotechnol. Adv. 13, 45-73.
10. DeKinkelin, P. (1972) Le virus D'Egtved. II. Purification. Ann. Rech. Vét. 3, 199-208.
11. DeKinkelin, P. (1988) Vaccination against viral haemorrhagic septicaemia. In: A.E. Ellis (Ed.). Fish Vaccination, Academic Press, New York, pp. 172-192.
12. Deuter, A. and Enzmann, P.J. (1986) Comparative biochemical and serological studies on two fish-pathogenic rhabdoviruses (VHS-V and SVC-V). J. Vet. Med. 33, 36-46.
13. Enzmann, H. and Konrad, M. (1993) Longevity of antibodies in brown trout and rainbow trout following experimental infection with VHS virus. Bull. Eur. Assoc. Fish Pathol. 13, 193-194.
14. Estepa, A. and Coll, J.M. (1995) Pepscan mapping and fusion-related properties of the major phosphatidylserine binding domain of the glycoprotein of VHSV. a salmonid rhabdovirus. Virology (in press).
15. Estepa, A., DeBlas, C., Ponz, F. and Coll, J.M. (1995) Detection of trout haemorrhagic septicaemia rhabdovirus by capture with monoclonal antibodies and amplification with PCR. Bull. Eur. Assoc. Fish Pathol. 15, 49-53.
16. Lorenzen, N., Olesen, N.J. and Jorgensen, P.E.V. (1988) Production and characterization of monoclonal antibodies to four Egtved virus structural proteins. Dis. Aquat. Org. 4, 35-42.
17. Lorenzen, N., Olesen, N.J. and Vestergaard-Jorgensen, P.E. (1990) Neutralization of Egtved virus pathogenicity to cell cultures and fish by monoclonal antibodies to the viral G protein. J. Gen. Virol. 71, 561-567.
18. Lorenzo, G.A., Estepa, A., Chylmonczyk, S. and Coll, J.M. (1995) Different peptides from hemorrhagic septicemia rhabdoviral protein stimulate leucocyte proliferation with individual fish variation. Virology 212 (in press).
19. McAllister, P.E. and Schill, W.B. (1986) Immunoblot assay: a rapid and sensitive method for identification of salmonid fish viruses. J. Wildl. Dis. 22, 468-474.
20. McAllister, P.E. and Wagner, R. (1975) Structural proteins of two salmonid rhabdoviruses. J. Virol. 15, 733-738.
21. Mourton, C., Bearzotti, M., Piechaczyk, M., Paolucci, F., Pau, B., Bastide, J.M. and Kinkelin, P.D. (1990) Antigen-capture ELISA for viral haemorrhagic septicaemia virus serotype I. J. Virol. Methods 29, 325-334.
22. Mourton, C., Romestand, B., DeKinkelin, P., Jeffroy, J., Le Gouvello, R. and Pau, B. (1992). Highly sensitive immunoassay for direct diagnosis of viral haemorrhagic septicaemia which uses antinucleocapsid monoclonal antibodies. J. Clin. Microbiol. 30, 2338-2345.
23. Olesen, N.J. Lorenzen, N. and Vestergaard-Jorgensen, P.E. (1991) Detection of rainbow trout antibody to Egtved virus by enzyme-linked immunosorbent assay (EEISA), immunofluorescence (IF), and plaque neutralization tests (50% PNT). Dis. Aquat. Org. 10, 31-38.
24. Olesen, N.J., Lorenzen, N. and Jorgensen, P.E.V. (1993) Serological differences among isolates of viral haemorrhagic septicaemia virus detected by neutralizing monoclonal and polyclonal antibodies. Dis. Aquat. Org. 16, 163-170.
25. Olesen, N.J. and Vestergard-Jorgensen, P.E. (1986) Detection of neutralizing antibody to Egtved virus in Rainbow trout (Salmo gairdneri) by plaque neutralization test with complement addition. J. Appl. Icthyol. 2, 33-41.
26. Sanz, F., Basurco, B., Babin, M., Dominguez, J. and Coll, J.M. (1993) Monoclonal antibodies against the structural proteins of viral haemorrhagic septicaemia virus isolates. J. Fish Dis. 16, 53-63.
27. Sanz, F. and Coll, J.M. (1992a) Techniques for the diagnosis of the viral diseases of salmonids. Dis. Aquat. Org. 13, 211-223.
28. Sanz, F.A. and Coll, J.M. (1992b) Detection of hemorrhagic virus of salmonid fishes by use of an enzyme-linked immunosorbent assay containing high sodium chloride concentration and two concompetitive monoclonal antibodies against early viral nucleoproteins. Am. J. Vet. Res. 53, 897-903.
29. Sanz, F. and Coll, J.M. (1992c) Detection of viral haemorrhagic septicemia virus by direct immunoperoxidase with selected anti-nucleoprotcin monoclonal antibody. Bull. Eur. Assoc. Fish Pathol. 12, 116-119.
30. Sanz, F. and Coll, J.M. (1992d) Neutralizing-enhancing monoclonal antibody recognizes the denatured glycoprotein of viral haemorrhagic septicaemia virus. Arch. Virol. 127, 223-232.