Critical conformational changes in the Arp2/3 complex are induced by nucleotide and nucleation promoting factor

Molecular Cell

Volumn 16, Issue 2, 2004, Pages 269-279

  Goley, Erin D ^a   Rodenbusch, Stacia E ^a   Martin, Adam C ^a   Welch, Matthew D ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; ACTIN BINDING PROTEIN; ACTIN RELATED PROTEIN 2; ACTIN RELATED PROTEIN 3; NUCLEATION PROMOTING FACTOR; NUCLEOTIDE; PROTEIN; UNCLASSIFIED DRUG; WISKOTT ALDRICH SYNDROME PROTEIN;

ARTICLE; BINDING AFFINITY; CONFORMATIONAL TRANSITION; PROTEIN BINDING;

ACTIN-RELATED PROTEIN 2; ACTIN-RELATED PROTEIN 3; ACTINS; CYTOSKELETAL PROTEINS; FLUORESCENCE RESONANCE ENERGY TRANSFER; LISTERIA; NUCLEOTIDES; PROTEIN CONFORMATION; PROTEINS; WISKOTT-ALDRICH SYNDROME PROTEIN;

VESPIDAE;

EID: 6344258806     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcel.2004.09.018     Document Type: Article

References (41)

1. Belmont L.D., Patterson G.M.L., Drubin D.G. New actin mutants allow further characterization of the nucleotide binding cleft and drug binding sites. J. Cell Sci. 112:1999;1325-1336
2. Beltzner C.C., Pollard T.D. Identification of functionally important residues of Arp2/3 complex by analysis of homology models from diverse species. J. Mol. Biol. 336:2004;551-565
3. Blanchoin L., Amann K.J., Higgs H.N., Marchand J.B., Kaiser D.A., Pollard T.D. Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins. Nature. 404:2000;1007-1011
4. Blanchoin L., Pollard T.D., Hitchcock-DeGregori S.E. Inhibition of the Arp2/3 complex-nucleated actin polymerization and branch formation by tropomyosin. Curr. Biol. 11:2001;1300-1304
5. Cooper J.A., Walker S.B., Pollard T.D. Pyrene actin. documentation of the validity of a sensitive assay for actin polymerization J. Muscle Res. Cell Motil. 4:1983;253-262
6. Cory G.O., Cramer R., Blanchoin L., Ridley A.J. Phosphorylation of the WASP-VCA domain increases its affinity for the Arp2/3 complex and enhances actin polymerization by WASP. Mol. Cell. 11:2003;1229-1239
7. Dayel M.J., Mullins R.D. Activation of Arp2/3 complex. addition of the first subunit of the new filament by a WASP protein triggers rapid ATP hydrolysis on Arp2 PLoS Biol. 2:2004;E91
8. Dayel M.J., Holleran E.A., Mullins R.D. Arp2/3 complex requires hydrolyzable ATP for nucleation of new actin filaments. Proc. Natl. Acad. Sci. USA. 98:2001;14871-14876
9. DesMarais V., Ichetovkin I., Condeelis J., Hitchcock-DeGregori S.E. Spatial regulation of actin dynamics. a tropomyosin-free, actin-rich compartment at the leading edge J. Cell Sci. 115:2002;4649-4660
10. Gournier H., Goley E.D., Niederstrasser H., Trinh T., Welch M.D. Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity. Mol. Cell. 8:2001;1041-1052
11. Griffin B.A., Adams S.R., Tsien R.Y. Specific covalent labeling of recombinant protein molecules inside live cells. Science. 281:1998;269-272
12. Higgs H.N., Blanchoin L., Pollard T.D. Influence of the C terminus of Wiskott-Aldrich syndrome protein (WASp) and the Arp2/3 complex on actin polymerization. Biochemistry. 38:1999;15212-15222
13. Ichetovkin I., Grant W., Condeelis J. Cofilin produces newly polymerized actin filaments that are preferred for dendritic nucleation by the Arp2/3 complex. Curr. Biol. 12:2002;79-84
14. Kabsch W., Mannherz H.G., Suck D., Pai E.F., Holmes K.C. Atomic structure of the actin:DNase I complex. Nature. 347:1990;37-44
15. Kelleher J.F., Atkinson S.J., Pollard T.D. Sequences, structural models, and cellular localization of the actin-related proteins Arp2 and Arp3 from Acanthamoeba. J. Cell Biol. 131:1995;385-397
16. Kouyama T., Mihashi K. Fluorimetry study of N-(1-pyrenyl)iodoacetamide- labelled F-actin. local structural change of actin protomer both on polymerization and on binding of heavy meromyosin Eur. J. Biochem. 114:1981;33-38
17. Le Clainche C., Didry D., Carlier M.F., Pantaloni D. Activation of Arp2/3 complex by Wiskott-Aldrich Syndrome protein is linked to enhanced binding of ATP to Arp2. J. Biol. Chem. 276:2001;46689-46692
18. Le Clainche C., Pantaloni D., Carlier M.F. ATP hydrolysis on actin-related protein 2/3 complex causes debranching of dendritic actin arrays. Proc. Natl. Acad. Sci. USA. 100:2003;6337-6342
19. Machesky L.M., Mullins R.D., Higgs H.N., Kaiser D.A., Blanchoin L., May R.C., Hall M.E., Pollard T.D. Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex. Proc. Natl. Acad. Sci. USA. 96:1999;3739-3744
20. Marchand J.B., Kaiser D.A., Pollard T.D., Higgs H.N. Interaction of WASP/Scar proteins with actin and vertebrate Arp2/3 complex. Nat. Cell Biol. 3:2001;76-82
21. Mullins R.D., Heuser J.A., Pollard T.D. The interaction of Arp2/3 complex with actin. nucleation, high affinity pointed end capping, and formation of branching networks of actin filaments Proc. Natl. Acad. Sci. USA. 95:1998;6181-6186
22. Panchal S.C., Kaiser D.A., Torres E., Pollard T.D., Rosen M.K. A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex. Nat. Struct. Biol. 10:2003;591-598
23. Pollard T.D., Beltzner C.C. Structure and function of the Arp2/3 complex. Curr. Opin. Struct. Biol. 12:2002;768-774
24. Pollard T.D., Borisy G.G. Cellular motility driven by assembly and disassembly of actin filaments. Cell. 112:2003;453-465
25. Rice S., Lin A.W., Safer D., Hart C.L., Naber N., Carragher B.O., Cain S.M., Pechatnikova E., Wilson-Kubalek E.M., Whittaker M., et al. A structural change in the kinesin motor protein that drives motility. Nature. 402:1999;778-784
26. Robinson R.C., Turbedsky K., Kaiser D.A., Marchand J.B., Higgs H.N., Choe S., Pollard T.D. Crystal structure of Arp2/3 complex. Science. 294:2001;1679-1684
27. Shih W.M., Gryczynski Z., Lakowicz J.R., Spudich J.A. A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin. Cell. 102:2000;683-694
28. Spudich J.A., Watt S. The regulation of rabbit skeletal muscle contraction. biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin J. Biol. Chem. 246:1971;4866-4871
29. Theriot J.A., Rosenblatt J., Portnoy D.A., Goldschmidt C.P., Mitchison T.J. Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts. Cell. 76:1994;505-517
30. Tsien R.Y. The Green Fluorescent Protein. Annu. Rev. Biochem. 67:1998;509-544
31. Uruno T., Liu J., Zhang P., Fan Y., Egile C., Li R., Mueller S.C., Zhan X. Activation of Arp2/3 complex-mediated actin polymerization by cortactin. Nat. Cell Biol. 3:2001;259-266
32. Volkmann N., Amann K.J., Stoilova-McPhie S., Egile C., Winter D.C., Hazelwood L., Heuser J.E., Li R., Pollard T.D., Hanein D. Structure of Arp2/3 complex in its activated state and in actin filament branch junctions. Science. 293:2001;2456-2459
33. Weaver A.M., Karginov A.V., Kinley A.W., Weed S.A., Li Y., Parsons J.T., Cooper J.A. Cortactin promotes and stabilizes Arp2/3-induced actin filament network formation. Curr. Biol. 11:2001;370-374
34. Weaver A.M., Heuser J.E., Karginov A.V., Lee W.L., Parsons J.T., Cooper J.A. Interaction of cortactin and N-WASp with Arp2/3 complex. Curr. Biol. 12:2002;1270-1278
35. Weed S.A., Karginov A.V., Schafer D.A., Weaver A.M., Kinley A.W., Cooper J.A., Parsons J.T. Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex. J. Cell Biol. 151:2000;29-40
36. Welch M.D., Mullins R.D. Cellular control of actin nucleation. Annu. Rev. Cell Dev. Biol. 18:2002;247-288
37. Welch M.D., Iwamatsu A., Mitchison T.J. Actin polymerization is induced by the Arp2/3 protein complex at the surface of Listeria monocytogenes. Nature. 385:1997;265-269
38. Wertman K.F., Drubin D.G., Botstein D. Systematic mutational analysis of the yeast ACT1 gene. Genetics. 132:1992;337-350
39. Yarar D., To W., Abo A., Welch M.D. The Wiskott-Aldrich Syndrome protein directs actin-based motility by stimulating actin nucleation with the Arp2/3 complex. Curr. Biol. 9:1999;555-558
40. Yarar D., D'Alessio J.A., Jeng R.L., Welch M.D. Motility determinants in WASP family proteins. Mol. Biol. Cell. 13:2002;4045-4059
41. Zalevsky J., Lempert L., Kranitz H., Mullins R.D. Different WASP family proteins stimulate different Arp2/3 complex-dependent actin-nucleating activities. Curr. Biol. 11:2001;1903-1913