Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins

Nature

Volumn 404, Issue 6781, 2000, Pages 1007-1011

  Blancholn, Laurent ^a   Amann, Kurt J ^a   Higgs, Henry N ^a   Marchand, Jean Baptiste ^a   Kaiser, Donald A ^a   Pollard, Thomas D ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; PROFILIN;

ARTICLE; CELL GROWTH; CELL MOTILITY; DENDRITE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN POLYMERIZATION;

ACANTHAMOEBA; ACTIN DEPOLYMERIZING FACTORS; ACTIN-RELATED PROTEIN 2; ACTIN-RELATED PROTEIN 3; ACTINS; ANIMALS; CATTLE; CONTRACTILE PROTEINS; CYTOSKELETAL PROTEINS; DESTRIN; HUMANS; MICROFILAMENT PROTEINS; MUSCLE, SKELETAL; NUCLEOTIDES; PROFILINS; PROTEINS; RABBITS; RECOMBINANT PROTEINS; WISKOTT-ALDRICH SYNDROME PROTEIN; WISKOTT-ALDRICH SYNDROME PROTEIN FAMILY;

EID: 0034720293     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35010008     Document Type: Article

References (28)

1. Wang, Y. Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling. J. Cell Biol. 101, 597-602 (1985).
2. Theriot, J. A. & Mitchison, T. J. Actin microfilament dynamics in locomoting cells. Nature 352, 126-131 (1991).
3. Svitkina, T. M., Verkhovsky, A. B., McQuade, K. M. & Borisy, G. G. Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation. J Cell Biol. 139, 397-415 (1997).
4. Mullins, R. D., Heuser, J. A. & Pollard, T. D. The interaction of Arp2/3 complex with actin: nucleation, high-affinity pointed end capping, and formation of branching networks of filaments. Proc. Natl Acad. Sci. USA 95, 6181-6186 (1998).
5. Pollard, T. D. in Guidebook to the Cytoskeletal and Motor Proteins 2nd edn (eds Kreis, T. & Vale, R.) 3-11 (1999).
6. Higgs, H. N. & Pollard, T. D. Regulation of actin polymerization by Arp2/3 complex and WASP/Scar proteins. J. Biol. Chem. 274, 32531-32534 (1999).
7. Loisel, T. P., Boujemaa, R., Pantaloni, D. & Carlier, M. F. Reconstitution of actin-based motility of Listeria and Shigella using pure proteins. Nature 401, 613-616 (1999).
8. Machesky, L. M. et al. Scar, A WASP-related protein, activates nucleation of actin filaments by the Arp2/3 complex. Proc. Natl Acad. Sci. USA 96, 3739-3744 (1999).
9. Higgs, H. N., Blanchoin, L. & Pollard, T. D. Influence of the Wiskott-Aldrich syndrome protein (WASP) C terminus and Arp2/3 complex on actin polymerization. Biochemistry 38, 15212-15222 (1999).
10. Svitkina, T. M. & Borisy, G. G. Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia. J. Cell Biol. 145, 1009-1026 (1999).
11. Pollard, T. D. & Cooper, J. A. Quantitative analysis of the effect of Acanthamoeba profilin on actin filament nucleation and elongation. Biochemistry 23, 6631-6641 (1984).
12. Schafer, D. A., Jennings, P. B. & Cooper, J. A. Dynamics of capping protein and actin assembly in vitro: uncapping barbed ends by polyphosphoinositides. J. Cell Biol. 135, 169-179 (1996).
13. Cooper, J. A. & Pollard, T. D. Effects of capping protein on the kinetics of actin polymerization. Biochemistry 24, 793-799 (1985).
14. Caldwell, J. E., Heiss, S. G., Mermall, V. & Cooper, J. A. Effects of CapZ, an actin capping protein of muscle, on the polymerization of actin. Biochemistry 28, 8506-8514 (1989).
15. Xu, J., Casella, J. F. & Pollard, T. D. Effect of capping protein, CapZ, on the length of actin filaments and mechanical properties of actin filament networks. Cell. Motil. Cytoskeleton 42, 73-81 (1999).
16. Dancker, P. & Hess, L. Phalloidin reduces the release of inorganic phosphate during actin polymerization. Biochim. Biophys. Acta 1035, 197-200 (1990).
17. Combeau, C. & Carlier, M. F. Characterization of the aluminum and beryllium fluoride species bound to F-actin and microtubules at the site of the g-phosphate of the nucleotide. J. Biol. Chem. 264, 19017-19021 (1989).
18. Mogilner, A. & Oster, G. Cell motility driven by actin polymerization. Biophys. J. 71, 303-345 (1996).
19. Blanchoin, L. & Pollard, T. D. Mechanism of interaction of Acanthamoeba actophorin (ADF/cofilin) with actin filaments. J. Biol. Chem. 274, 15538-15546 (1999).
20. Maciver, S. K., Zot, H. G. & Pollard, T. D. Characterization of actin filament severing by actophorin from Acanthamoeba castellanii. J. Cell Biol. 115, 1611-1620 (1991).
21. Carlier, M. F. et al. Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: implication in actin-based motility. J. Cell Boil. 136, 1307-1322 (1997).
22. Spudich, J. A. & Watt, S. The regulation of rabbit skeletal muscle contraction. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proleolytic fragments of myosin. J. Biol. Chem. 246, 4866-4871 (1971).
23. Pollard, T. D. Polymerization of ADP-actin. J. Cell Biol. 99, 769-777 (1984).
24. Machesky, L. M., Atkinson, S. J., Ampe, C., Vandekerckhove, J. & Pollard, T. D. Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin agarose. J. Cell Biol. 127, 107-115 (1994).
25. Cooper, J. A., Blum, J. D. & Pollard, T. D. Acanthamoeba castellanii capping protein: properties, mechanism of action, immunologic cross-reactivity, and localization. J. Cell Biol. 99, 217-225 (1984).
26. Kaiser, D. A., Goldschmidt-Clermont, P. J., Levine, B. A. & Pollard, T. D. Characterization of renatured profilin purified by urea elution from poly-L-proline agarose columns. Cell Motil. 14, 251-262 (1989).
27. Almo, S. C., Pollard, T. D., Way, M. & Lattman, E. E. Purification, characterization and crystallization of Acanthamoeba profilin expressed in Eschericia coli. J. Mol. Biol. 236, 950-952 (1994).
28. Kouyama, T. & Mihashi, K. Fluorimetry study of N-(1-pyrenyl) iodoacetamide-labelled F-actin. Local structural change of actin protomer both on polymerization and on binding of heavy meromyosin. Eur. J. Biochem. 114, 33-38 (1981).