Knockdown of endogenous SKIP gene enhanced insulin-induced glycogen synthesis signaling in differentiating C2C12 myoblasts

BMB Reports

Volumn 42, Issue 2, 2009, Pages 119-124

  Xiong, Qi ^a   Deng, Chang Yan ^a   Chai, Jin ^a   Jiang, Si Wen ^a   Xiong, Yuan Zhu ^a   Li, Feng E ^a   Zheng, Rong ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Akt; Glycogen synthase; GSK3 ; RNAi; SKIP

Indexed keywords

GLYCOGEN; GLYCOGEN SYNTHASE KINASE 3; GLYCOGEN SYNTHASE KINASE 3 BETA; INSULIN; PHOSPHATASE; PPS PROTEIN, MOUSE; PROTEIN KINASE B;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL DIFFERENTIATION; CELL LINE; CYTOLOGY; GENE SILENCING; GENETICS; METABOLISM; MOUSE; MYOBLAST; PHYSIOLOGY; RNA INTERFERENCE; SIGNAL TRANSDUCTION;

ANIMALS; CELL DIFFERENTIATION; CELL LINE; GENE KNOCKDOWN TECHNIQUES; GLYCOGEN; GLYCOGEN SYNTHASE KINASE 3; INSULIN; MICE; MYOBLASTS; PHOSPHORIC MONOESTER HYDROLASES; PROTO-ONCOGENE PROTEINS C-AKT; RNA INTERFERENCE; SIGNAL TRANSDUCTION;

EID: 63049093727     PISSN: 19766696     EISSN: 1976670X     Source Type: Journal    
DOI: 10.5483/BMBRep.2009.42.2.119     Document Type: Article

References (25)

1. Rosenvold, K., Petersen, J. S., Lwerke, H. N., Jensen, S. K., Therkildsen, M., Karlsson, A. H., Møller, H. S. and Andersen, H. J. (2001) Muscle glycogen stores and meat quality as affected by strategic finishing feeding of slaughter pigs. J. Anim. Sci. 79, 382-391
2. Czech, M. P. and Corvera, S. (1999) Signaling mechanisms that regulate glucose transport. J. Biol. Chem. 274, 1865-1868.
3. Toker, A. S. and Macnab, R. M. (1997) Signalling through the lipid products of phosphoinositide-3-OH kinase. Nature 387, 673-676.
4. Ijuin, T., Mochizuki, Y., Fukami, K., Funaki, M., Asano, T. and Takenawa, T. (2000) Identification and characterization of a novel inositol polyphosphate 5-phosphatase. J. Biol. Chem. 275, 10870-10875.
5. Ijuin, T. and Takenawa, T. (2003) SKIP negatively regulates insulin-induced GLUT4 translocation and membrane ruffle formation. Mol. Cell Biol. 23, 1209-1220.
6. Saltiel, A. R. (1996) Diverse signaling pathways in the cellular actions of insulin. Am. J. Physiol. 270, 375-385.
7. Stephens, L., Anderson, K., Stokoe, D., Erdjument-Bromage, H., Painter, G. F., Holmes, A. B., Gaffney, P. R., Reese, C. B., McCormick, F., Tempst, P., Coadwell, J. and Hawkins, P. T. (1998) Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B. Science 279, 710-714.
8. Manning, B. D. and Cantley, L. C. (2007) AKT/PKB signaling, navigating downstream. Cell 129, 1261-1274.
9. Kang, H.Y., Shim, D., Kang, S.S., Chang, S.I. and Kim, H.Y. (2006) Protein kinase B inhibits endostatin-induced apoptosis in HUVECs. J. Biochem. Mol. Biol. 39, 97-104.
10. McManus, E. J., Sakamoto, K., Armit, L. J., Ronaldson, L., Shpiro, N., Marquez, R. and Alessi, D. R. (2005) Role that phosphorylation of GSK3 plays in insulin and Wnt signalling defined by knockin analysis. EMBO. J. 24, 1571-1583.
11. Macaulay, K., Blair, A. S., Hajduch, E., Terashima, T., Baba, O., Sutherland, C. and Hundal, H. S. (2005) Constitutive activation of GSK3 down regulates glycogen synthase abundance and glycogen deposition in rat skeletal muscle cells. J. Biol. Chem. 280, 9509-9518.
12. Reynolds, A., Leake, D., Boese, Q., Scaringe, S., Marshall, W. S. and Khvorova, A. (2004) Rational siRNA design for RNA interference. Nat. Biotechnol. 22, 326-330.
13. Kim, J., Kim, H., Lee, Y., Yang, K., Byun, S. and Han, K. (2006) A simple and economical short-oligonucleotide-based approach to shRNA generation. J. Biochem. Mol. Biol. 39, 329-334.
14. Andjelković, M., Alessi, D. R., Meier, R., Fernandez, A., Lamb, N. J., Frech, M., Cron, P., Cohen, P., Lucocq, J. M. and Hemmings, B. A. (1997) Role of translocation in the activation and function of protein kinase B. J. Biol. Chem. 272, 31515-31524.
15. Ma, K., Cheung, S.M., Marshall, A.J. and Duronio, V. (2008) PI(3,4,5)P3 and PI(3,4)P2 levels correlate with PKB/akt phosphorylation at Thr308 and Ser473, respectively; PI(3,4) P2 levels determine PKB activity. Cell Signal. 20, 684-694.
16. Summers, S. A., Kao, A. W., Kohn, A. D., Backus, G. S., Roth, R. A., Pessin, J. E. and Birnbaum, M. J. (1999) The role of glycogen synthase kinase 3beta in insulin- stimulated glucose metabolism. J. Biol. Chem. 274, 17934- 17940.
17. Munro, S., Ceulemans, H., Bollen, M., Diplexcito, J. and Cohen, P.T. (2005) A novel glycogen-targeting subunit of protein phosphatase 1 that is regulated by insulin and shows differential tissue distribution in humans and rodents. FEBS. J. 272, 1478-1489.
18. Ijuin, T., Yu, Y.E., Mizutani, K., Pao, A., Tateya, S., Tamori, Y., Bradley, A. and Takenawa, T. (2008) Increased insulin action in SKIP heterozygous knockout mice. Mol. Cell Biol. 28, 5184-5195.
19. Fukui, K., Wada, T., Kagawa, S., Nagira, K., Ikubo, M., Ishihara, H., Kobayashi, M. and Sasaoka, T. (2005) Impact of the liver-specific expression of SHIP2 (SH2-containing inositol 5'-phosphatase 2) on insulin signaling and glucose metabolism in mice. Diabetes 54, 1958-1967.
20. Sasaoka, T., Hori, H., Wada, T., Ishiki, M., Haruta, T., Ishihara, H. and Kobayashi, M. (2001) SH2-containing inositol phosphatase 2 negatively regulates insulin-induced glycogen synthesis in L6 myotubes. Diabetologia 44, 1258-1267.
21. Wada, T., Sasaoka, T., Funaki, M., Hori, H., Murakami, S., Ishiki, M., Haruta, T., Asano, T., Ogawa, W., Ishihara, H. and Kobayashi, M. (2001) Overexpression of SH2- containing inositol phosphatase 2 results in negative regulation of insulin-induced metabolic actions in 3T3-L1 adipocytes via its 5'-phosphatase catalytic activity. Mol. Cell Biol. 21, 1633-1646.
22. Sleeman, M. W., Wortley, K. E., Lai, K. M., Gowen, L.C., Kintner, J., Kline, W. O., Garcia, K., Stitt, T. N., Yancopoulos, G. D., Wiegand, S. J. and Glass, D. J. (2005) Absence of the lipid phosphatase SHIP2 confers resistance to dietary obesity. Nat. Med. 11, 199-205.
23. Cantley, L.C. and Neel, B. G. (1999) New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway. Proc. Natl. Acad. Sci. U.S.A. 96, 4240-4245.
24. Tang, X., Powelka, A. M., Soriano, N. A., Czech, M. P. and Guilherme, A. (2005) PTEN but not SHIP2 suppresses insulin signaling through the phosphatidylinositol 3-kinase/Akt pathway in 3T3-L1 adipocytes. J. Biol. Chem. 280, 22523-22529.
25. Wijesekara, N., Konrad, D., Eweida, M., Jefferies, C., Liadis, N., Giacca, A., Crackower, M., Suzuki, A., Mak, T. W., Kahn, C. R., Klip, A. and Woo, M. (2005) Muscle-specific Pten deletion protects against insulin resistance and diabetes. Mol. Cell Biol. 25, 1135-1145.