Pathways of adipose tissue androgen metabolism in women: Depot differences and modulation by adipogenesis

American Journal of Physiology - Endocrinology and Metabolism

Volumn 296, Issue 2, 2009, Pages

  Blouin, Karine ^a   Nadeau, Mélanie ^a   Mailloux, Jacques ^a   Daris, Marleen ^a   Lebel, Stephane ^a   Luu The, Van ^a   Tchernof, André ^a  

^a UNIVERSITÉ LAVAL   (Canada)

Author keywords

Adipocyte differentiation; Aldo keto reductases; Estrogen; Omental visceral fat; Short chain dehydrogenases

Indexed keywords

3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; ALDO KETO REDUCTASE 1C2; ALDO KETO REDUCTASE 1C3; ANDROGEN RECEPTOR; ANDROSTANOLONE; AROMATASE; DEXAMETHASONE; ESTROGEN RECEPTOR; GLUCOCORTICOID RECEPTOR; MESSENGER RNA; OXIDOREDUCTASE; RETINOL DEHYDROGENASE; RNA; STERYL SULFATASE; UNCLASSIFIED DRUG; 15 HYDROXYPROSTAGLANDIN DEHYDROGENASE; 17 HYDROXYSTEROID DEHYDROGENASE II, HUMAN; 17-HYDROXYSTEROID DEHYDROGENASE II, HUMAN; 3 (OR 17) BETA HYDROXYSTEROID DEHYDROGENASE; 3 (OR 17)-BETA-HYDROXYSTEROID DEHYDROGENASE; AKR1C2 PROTEIN, HUMAN; AKR1C3 PROTEIN, HUMAN; ANDROGEN; HYDROXYSTEROID DEHYDROGENASE;

ABDOMINAL SUBCUTANEOUS FAT; ADIPOGENESIS; ADIPOSE TISSUE; ANDROGEN METABOLISM; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; FEMALE; HORMONAL REGULATION; HUMAN; HUMAN CELL; HUMAN TISSUE; MOLECULAR INTERACTION; PRIORITY JOURNAL; PROADIPOCYTE; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; ABDOMINAL FAT; ADULT; BIOLOGICAL MODEL; BODY FAT DISTRIBUTION; COMPARATIVE STUDY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MIDDLE AGED; OMENTUM; PHYSIOLOGY; SUBCUTANEOUS FAT;

17-HYDROXYSTEROID DEHYDROGENASES; 3-HYDROXYSTEROID DEHYDROGENASES; ABDOMINAL FAT; ADIPOGENESIS; ADIPOSE TISSUE; ADULT; ANDROGENS; BODY FAT DISTRIBUTION; CELLS, CULTURED; FEMALE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; HYDROXYPROSTAGLANDIN DEHYDROGENASES; HYDROXYSTEROID DEHYDROGENASES; METABOLIC NETWORKS AND PATHWAYS; MIDDLE AGED; MODELS, BIOLOGICAL; OMENTUM; SUBCUTANEOUS FAT;

EID: 60849102451     PISSN: 01931849     EISSN: 15221555     Source Type: Journal    
DOI: 10.1152/ajpendo.00039.2008     Document Type: Article

References (44)

1. Anderson LA, McTernan PG, Barnett AH, Kumar S. The effects of androgens and estrogens on preadipocyte proliferation in human adipose tissue: influence of gender and site. J Clin Endocrinol Metab 86: 5045-5051, 2001. (Pubitemid 33020562)
2. Bélanger C, Hould FS, Lebel S, Biron S, Brochu G, Tchernof A. Omental and subcutaneous adipose tissue steroid levels in obese men. Steroids 71: 674-682, 2006. (Pubitemid 43994015)
3. Bélanger C, Luu-The V, Dupont P, Tchernof A. Adipose tissue intracrinology: potential importance of local androgen/estrogen metabolism in the regulation of adiposity. Horm Metab Res 34: 737-745, 2002. (Pubitemid 36398717)
4. Blanchette S, Blouin K, Richard C, Dupont P, Luu-The V, Tchernof A. Expression and activity of 20alpha-hydroxysteroid dehydrogenase (AKR1C1) in abdominal subcutaneous and omental adipose tissue in women. J Clin Endocrinol Metab 90: 264-270, 2005. (Pubitemid 40116667)
5. Blouin K, Blanchette S, Richard C, Dupont P, Luu-The V, Tchernof A. Expression and activity of steroid aldoketoreductases 1C in omental adipose tissue are positive correlates of adiposity in women. Am J Physiol Endocrinol Metab 288: E398-E404, 2005. (Pubitemid 40110649)
6. Blouin K, Richard C, Bélanger C, Dupont P, Daris M, Laberge P, Luu-The V, Tchernof A. Local androgen inactivation in abdominal visceral adipose tissue. J Clin Endocrinol Metab 88: 5944-5950, 2003. (Pubitemid 38033076)
7. Blouin K, Richard C, Brochu G, Hould FS, Lebel S, Marceau S, Biron S, Luu-The V, Tchernof A. Androgen inactivation and steroid-converting enzyme expression in abdominal adipose tissue in men. J Endocrinol 191: 637-649, 2006. (Pubitemid 46210289)
8. Bujalska IJ, Kumar S, Stewart PM. Does central obesity reflect "Cushing's disease of the omentum"? Lancet 349: 1210-1213, 1997.
9. Bujalska IJ, Walker EA, Hewison M, Stewart PM. A switch in dehydrogenase to reductase activity of 11 beta-hydroxysteroid dehydrogenase type 1 upon differentiation of human omental adipose stromal cells. J Clin Endocrinol Metab 87: 1205-1210, 2002. (Pubitemid 36121089)
10. Corbould AM, Bawden MJ, Lavranos TC, Rodgers RJ, Judd SJ. The effect of obesity on the ratio of type 3 17beta-hydroxysteroid dehydrogenase mRNA to cytochrome P450 aromatase mRNA in subcutaneous abdominal and intra-abdominal adipose tissue of women. Int J Obes Relat Metab Disord 26: 165-175, 2002.
11. Corbould AM, Judd SJ, Rodgers RJ. Expression of types 1, 2, and 3 17 beta-hydroxysteroid dehydrogenase in subcutaneous abdominal and intra-abdominal adipose tissue of women. J Clin Endocrinol Metab 83: 187-194, 1998.
12. Crandall DL, Busler DE, Novak TJ, Weber RV, Kral JG. Identification of estrogen receptor beta RNA in human breast and abdominal subcutaneous adipose tissue. Biochem Biophys Res Commun 248: 523-526, 1998.
13. Dieudonné MN, Leneveu MC, Giudicelli Y, Pecquery R. Evidence for functional estrogen receptors alpha and beta in human adipose cells: regional specificities and regulation by estrogens. Am J Physiol Cell Physiol 286: C655-C661, 2004. (Pubitemid 38292672)
14. Dieudonné MN, Pecquery R, Boumediene A, Leneveu MC, Giudicelli Y. Androgen receptors in human preadipocytes and adipocytes: regional specificities and regulation by sex steroids. Am J Physiol Cell Physiol 274: C1645-C1652, 1998. (Pubitemid 28304202)
15. Dieudonné MN, Sammari A, Dos SE, Leneveu MC, Giudicelli Y, Pecquery R. Sex steroids and leptin regulate 11beta-hydroxysteroid dehydrogenase I and P450 aromatase expressions in human preadipocytes: sex specificities. J Steroid Biochem Mol Biol 99: 189-196, 2006. (Pubitemid 43786264)
16. Dufort I, Labrie F, Luu-The V. Human types 1 and 3 3 alpha-hydroxysteroid dehydrogenases: differential lability and tissue distribution. J Clin Endocrinol Metab 86: 841-846, 2001. (Pubitemid 32208285)
17. 16a.Gupta V, Bhasin S, Guo W, Singh R, Miki R, Chauhan P, Choong K, Tchkonia T, Lebrasseur NK, Flanagan JN, Hamilton JA, Viereck JC, Narula NS, Kirkland JL, Jasuja R. Effects of dihydrotestosterone on differentiation and proliferation of human mesenchymal stem cells and preadipocytes. Mol Cell Endocrinol. In press.
18. Joyner J, Hutley L, Cameron D. Intrinsic regional differences in androgen receptors and dihydrotestosterone metabolism in human preadipocytes. Horm Metab Res 34: 223-228, 2002. (Pubitemid 34666583)
19. Joyner JM, Hutley LJ, Cameron DP. Estrogen receptors in human preadipocytes. Endocrine 15: 225-230, 2001.
20. Katz JR, Mohamed-Ali V, Wood PJ, Yudkin JS, Coppack SW. An in vivo study of the cortisol-cortisone shuttle in subcutaneous abdominal adipose tissue. Clin Endocrinol (Oxf) 50: 63-68, 1999. (Pubitemid 29079369)
21. Kershaw EE, Flier JS. Adipose tissue as an endocrine organ. J Clin Endocrinol Metab 89: 2548-2556, 2004.
22. Labrie F. Intracrinology. Mol Cell Endocrinol 78: 113-118, 1991.
23. Labrie F, Bélanger A, Cusan L, Candas B. Physiological changes in dehydroepiandrosterone are not reflected by serum levels of active androgens and estrogens but of their metabolites: intracrinology. J Clin Endocrinol Metab 82: 2403-2409, 1997. (Pubitemid 27337847)
24. Luu-The V, Paquet N, Calvo E, Cumps J. Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction. Biotechniques 38: 287-293, 2005. (Pubitemid 40216386)
25. Masuzaki H, Paterson J, Shinyama H, Morton NM, Mullins JJ, Seckl JR, Flier JS. A transgenic model of visceral obesity and the metabolic syndrome. Science 294: 2166-2170, 2001. (Pubitemid 33150673)
26. McTernan PG, Anderson LA, Anwar AJ, Eggo MC, Crocker J, Barnett AH, Stewart PM, Kumar S. Glucocorticoid regulation of p450 aromatase activity in human adipose tissue: gender and site differences. J Clin Endocrinol Metab 87: 1327-1336, 2002. (Pubitemid 36121105)
27. Palin SL, McTernan PG, Anderson LA, Sturdee DW, Barnett AH, Kumar S. 17Beta-estradiol and anti-estrogen ICI:compound 182,780 regulate expression of lipoprotein lipase and hormone-sensitive lipase in isolated subcutaneous abdominal adipocytes. Metabolism 52: 383-388, 2003. (Pubitemid 36775326)
28. Pedersen SB, Bruun JM, Hube F, Kristensen K, Hauner H, Richelsen B. Demonstration of estrogen receptor subtypes alpha and beta in human adipose tissue: influences of adipose cell differentiation and fat depot localization. Mol Cell Endocrinol 182: 27-37, 2001. (Pubitemid 32743439)
29. Pedersen SB, Fuglsig S, Sjogren P, Richelsen B. Identification of steroid receptors in human adipose tissue. Eur J Clin Invest 26: 1051-1056, 1996. (Pubitemid 27023520)
30. Quinkler M, Sinha B, Tomlinson JW, Bujalska IJ, Stewart PM, Arlt W. Androgen generation in adipose tissue in women with simple obesity - a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5. J Endocrinol 183: 331-342, 2004. (Pubitemid 39562714)
31. Ramirez-Zacarias JL, Castro-Munozledo F, Kuri-Harcuch W. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97: 493-497, 1992.
32. Rodbell M. Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J Biol Chem 239: 375-380, 1964.
33. Singh R, Artaza JN, Taylor WE, Braga M, Yuan X, Gonzalez-Cadavid NF, Bhasin S. Testosterone inhibits adipogenic differentiation in 3T3-L1 cells: nuclear translocation of androgen receptor complex with beta-catenin and T-cell factor 4 may bypass canonical Wnt signaling to down-regulate adipogenic transcription factors. Endocrinology 147: 141-154, 2006. (Pubitemid 43004269)
34. Singh R, Artaza JN, Taylor WE, Gonzalez-Cadavid NF, Bhasin S. Androgens stimulate myogenic differentiation and inhibit adipogenesis in C3H 10T1/2 pluripotent cells through an androgen receptor-mediated pathway. Endocrinology 144: 5081-5088, 2003. (Pubitemid 37378423)
35. Sottile V, Seuwen K. A high-capacity screen for adipogenic differentiation. Anal Biochem 293: 124-128, 2001.
36. Steckelbroeck S, Jin Y, Gopishetty S, Oyesanmi B, Penning TM. Human cytosolic 3alpha-hydroxysteroid dehydrogenases of the aldo-keto reductase superfamily display significant 3beta-hydroxysteroid dehydrogenase activity: implications for steroid hormone metabolism and action. J Biol Chem 279: 10784-10795, 2004. (Pubitemid 38379543)
37. Tchkonia T, Giorgadze N, Pirtskhalava T, Tchoukalova Y, Karagiannides I, Forse RA, DePonte M, Stevenson M, Guo W, Han J, Waloga G, Lash TL, Jensen MD, Kirkland JL. Fat depot origin affects adipogenesis in primary cultured and cloned human preadipocytes. Am J Physiol Regul Integr Comp Physiol 282: R1286-R1296, 2002. (Pubitemid 34664512)
38. Tchkonia T, Lenburg M, Thomou T, Giorgadze N, Frampton G, Pirtskhalava T, Cartwright A, Cartwright M, Flanagan J, Karagiannides I, Gerry N, Forse RA, Tchoukalova Y, Jensen MD, Pothoulakis C, Kirkland JL. Identification of depot-specific human fat cell progenitors through distinct expression profiles and developmental gene patterns. Am J Physiol Endocrinol Metab 292: E298-E307, 2007. (Pubitemid 46052953)
39. Valle LD, Toffolo V, Nardi A, Fiore C, Bernante P, Di Liddo R, Parnigotto PP, Colombo L. Tissue-specific transcriptional initiation and activity of steroid sulfatase complementing dehydroepiandrosterone sulfate uptake and intracrine steroid activations in human adipose tissue. J Endocrinol 190: 129-139, 2006. (Pubitemid 44161068)
40. Van Schothorst EM, Franssen-van Hal N, Schaap MM, Pennings J, Hoebee B, Keijer J. Adipose gene expression patterns of weight gain suggest counteracting steroid hormone synthesis. Obes Res 13: 1031-1041, 2005.
41. Van Harmelen V, Skurk T, Hauner H. Primary culture and differentiation of human adipocyte precursor cells. Methods Mol Med 107: 125-135, 2005.
42. Wake DJ, Strand M, Rask E, Westerbacka J, Livingstone DE, Soderberg S, Andrew R, Yki-Jarvinen H, Olsson T, Walker BR. Intraadipose sex steroid metabolism and body fat distribution in idiopathic human obesity. Clin Endocrinol (Oxf) 66: 440-446, 2007. (Pubitemid 46239306)
43. Yang K, Khalil MW, Strutt BJ, Killinger DW. 11 beta-Hydroxysteroid dehydrogenase 1 activity and gene expression in human adipose stromal cells: effect on aromatase activity. J Steroid Biochem Mol Biol 60: 247-253, 1997. (Pubitemid 27229780)
44. Zhang Y, Dufort I, Rheault P, Luu-The V. Characterization of a human 20alpha-hydroxysteroid dehydrogenase. J Mol Endocrinol 25: 221-228, 2000.