Dihydroorotase from the hyperthermophile Aquifiex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis

Biochemistry

Volumn 48, Issue 4, 2009, Pages 766-778

  Zhang, Pengfei ^a   Martin, Philip D ^a   Purcarea, Cristina ^a   Vaishnav, Asmita ^a   Brunzelle, Joseph S ^c   Fernando, Roshini ^d   Guy Evans, Hedeel I ^d   Evans, David R ^a   Edwards, Brian F P ^a,b  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b INSTITUTE OF BIOLOGY   (Romania)

^c NORTHWESTERN UNIVERSITY   (United States)

^d EASTERN MICHIGAN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITES; AQUIFEX AEOLICUS; ASPARTATE; CARBAMOYL ASPARTATE; CARBAMOYL PHOSPHATES; CYTOSOL; DIHYDROOROTASE; DODECAMER; HEXAMER; HYPERTHERMOPHILE; ISOSTERIC; MULTIENZYME COMPLEXES; MULTIFUNCTIONAL COMPLEXES; NONCOVALENT; ONE POTS; PEPTIDE BONDS; POLYPEPTIDE CHAINS; PYRIMIDINE BIOSYNTHESIS; REACTION CHAMBERS; SYNTHETASE; THREE-DIMENSIONAL STRUCTURES; ZINC ATOMS;

AMINES; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; BIOSYNTHESIS; CATALYSTS; COMPUTER AIDED DESIGN; ENZYMES; ESCHERICHIA COLI; MAMMALS; THREE DIMENSIONAL; ZINC;

CRYSTAL STRUCTURE;

ASPARTATE CARBAMOYLTRANSFERASE; ASPARTIC ACID; CARBAMOYL PHOSPHATE; CARBAMOYL PHOSPHATE SYNTHASE; CITRIC ACID; DIHYDROOROTASE; GLYCINE; POLYPEPTIDE; PYRIMIDINE; ZINC; 4,5 DIHYDROOROTIC ACID; 4,5-DIHYDROOROTIC ACID; BACTERIAL PROTEIN; DRUG DERIVATIVE; MULTIENZYME COMPLEX; OROTIC ACID; PYRIMIDINE DERIVATIVE;

AQUIFEX AEOLICUS; ARTICLE; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; PYRIMIDINE SYNTHESIS; REACTOR; STEREOSPECIFICITY; STOICHIOMETRY; ALLOSTERISM; BACTERIUM; BINDING SITE; BIOSYNTHESIS; CHEMISTRY; COMPARATIVE STUDY; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; STATIC ELECTRICITY; THERMODYNAMICS; X RAY CRYSTALLOGRAPHY;

AQUIFEX AEOLICUS; ESCHERICHIA COLI; MAMMALIA; PROKARYOTA;

ALLOSTERIC REGULATION; ASPARTATE CARBAMOYLTRANSFERASE; BACTERIA; BACTERIAL PROTEINS; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; DIHYDROOROTASE; MULTIENZYME COMPLEXES; OROTIC ACID; PROTEIN STRUCTURE, TERTIARY; PYRIMIDINES; STATIC ELECTRICITY; THERMODYNAMICS;

EID: 60749123877     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi801831r     Document Type: Article

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