Detection and location of the enzymes of de novo pyrimidine biosynthesis in mammalian spermatozoa

Reproduction

Volumn 123, Issue 6, 2002, Pages 757-768

  Carrey, E A ^a   Dietz, C ^a   Glubb, D M ^a   Loffler M ^a   Lucocq, J M ^a   Watson, P F ^a  

^a GUY S HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTATE CARBAMOYLTRANSFERASE; CARBAMOYL PHOSPHATE SYNTHASE (GLUTAMINE HYDROLYSING); DIHYDROOROTASE; DIHYDROOROTATE DEHYDROGENASE; MULTIENZYME COMPLEX; POLYPEPTIDE; PYRIMIDINE; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG; URIDINE PHOSPHATE; URIDINE PHOSPHATE SYNTHASE;

ANIMAL CELL; ARTICLE; CATTLE; CELL NUCLEUS; CELL STRAIN BHK; CONTROLLED STUDY; CYTOPLASM; CYTOSKELETON; DROSOPHILA; ENZYME ASSAY; ENZYME LOCALIZATION; HUMAN; HUMAN CELL; HYPOTHESIS; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE MICROSCOPY; MALE; MAMMAL CELL; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; PYRIMIDINE SYNTHESIS; SPERMATOZOON; WESTERN BLOTTING;

ANIMALIA; BOS TAURUS; CRICETINAE; MAMMALIA;

EID: 0036072433     PISSN: 14701626     EISSN: None     Source Type: Journal    
DOI: 10.1530/rep.0.1230757     Document Type: Article

References (43)

1. Adenovirus preterminal protein binds to the CAD enzyme at active sites of viral DNA replication on the nuclear matrix
2. Oocyte activation: Lessons from human infertility
3. Mapping of catalytic domains and phosphorylation sites in the multifunctional pyrimidine-biosynthetic protein CAD European
4. Intracellular location of the multi-domain protein CAD in mammalian cells
5. The cellular location of dihydroorotate dehydrogenase: Relation to de novo biosynthesis of pyrimidines
6. Channelling of urea cycle intermediates in situ in permeabilized hepatocytes
7. Purification from hamster cells of the multifunctional protein that initiates de novo synthesis of pyrimidine nucleotides
8. Analysis of the phenotypes exhibited by r-I mutants of D. melanogaster
9. Sperm structure and function
10. Mitogen-and stress-activated protein kinase-1 (MSK-1) a novel two-kinase domain enzyme that is directly activated by MAPK and SAPK2/p38 and which may mediate the activation of CREB EMBO
11. Immunocytochemical detection of mitochondrial dihydroorotate dehydrogenase in human spermatozoa
12. Activation of development in mammals: Is there a role for sperm cytoplasmic factor?
13. Isolation and characterization of the orotidine 5′-monophosphate decarboxylase domain of the multifunctional protein uridine 5′-monophosphate synthase
14. Cellular and molecular biology of capacitation and acrosome reaction in spermatozoa
15. Cloning and expression of the mammalian multifunctional protein CAD in Escherichia coli
16. Human uridine monophosphate synthase: Baculovirus expression, immuno-affinity column purification and characterisation of the acetylated amino terminus
17. Immunoaffinity purification of antibodies
18. Capacitation mechanisms, and the role of capacitation as seen in eutherian mammals
19. A reciprocal allosteric mechanism for efficient transfer of labile intermediates between active sites in CAD, the mammalian pyrimidine-biosynthetic multienzyme polypeptide
20. 2+ release in sea urchin egg homogenates
21. Stable mutants of mammalian cells that overproduce the first three enzymes of pyrimidine nucleotide biosynthesis
22. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
23. Catalytic enzyme histochemistry and biochemical analysis of dihydroorotate dehydrogenase/ oxidase and succinate dehydrogenase in mammalian tissues, cells and mitochondria
24. Particulate markers for immunoelectron microscopy
25. Quantitation of gold labelling and antigens in immunolabelled ultrathin sections
26. A specific nutritional requirement for pyrimidines in rudimentary mutants of D. melanogaster
27. Copurification of vimentin, energy metabolism enzymes, and a MER5 homolog with nucleoside diphosphate kinase. Identification of tissue-specific interactions
28. 2+ oscillations after injection into mouse egg
29. Cytoskeletal association of the A and B nucleoside diphosphate kinases of interphasic but not mitotic human carcinoma cell lines: Specific nuclear localisation of the B subunit
30. Expression of de novo pyrimidine biosynthesis genes during spermatogenesis in D. melanogaster
31. Requirements for the mitochondrial import and localisation of dihydroorotate dehydrogenase
32. Sperm motility and interactions among seminal uridine, xanthine, urate and ATPase in fertile and infertile men
33. Uridine phosphorylase association with vimentin. Intracellular distribution and localisation
34. The centrosome and its mode of inheritance: The reduction of the centrosome during gametogenesis and its restoration during fertilisation
35. Comparative modelling of mammalian aspartate transcarbamylase Proteins: Structure
36. Regulation of the mammalian carbamoyl-phosphate synthetase II by effectors and phosphorylation
37. Molecular cloning and nucleotide sequence for the complete coding region of human UMP synthase
38. A cytosolic sperm factor stimulates repetitive calcium increases and mimics fertilisation in hamster eggs
39. Targeting of a germ cell-specific type 1 hexokinase lacking a porin-binding domain to the mitochondria as well as to the head and fibrous sheath of murine spermatozoa
40. Functionalised relationships between capacitation-dependent cell signaling and compartmentalised metabolic pathways in murine spermatozoa
41. The urea cycle: A two-compartment system Essays in Biochemistry
42. Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa
43. Expression of the dihydroorotate dehydrogenase gene, dhod, during spermatogenesis in D. melanogaster