DNA-mediated charge transport requires conformational motion of the DNA bases: Elimination of charge transport in rigid glasses at 77 K

Journal of the American Chemical Society

Volumn 126, Issue 41, 2004, Pages 13234-13235

  O'Neill, Melanie A ^a   Barton, Jacqueline K ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DNA BASE; GLASS;

ANISOTROPY; ARTICLE; CONFORMATIONAL TRANSITION; DNA CONFORMATION; ION TRANSPORT; LOW TEMPERATURE; MOLECULAR DYNAMICS;

2-AMINOPURINE; COLD; DNA; FLUORESCENCE POLARIZATION; NUCLEIC ACID CONFORMATION; SPECTROMETRY, FLUORESCENCE;

EID: 6044228211     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0455897     Document Type: Article

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28. Oligonucleotides were prepared on an ABI synthesizer, HPLC purified, and analyzed by mass spectrometry. Duplexes (25 μM) were annealed in 100 mM NaCl pH 7, dried and resuspended in 10 M LiCl, pH 7.
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31. 2 yielding stable, uniform glasses.
32. The maximum anisotropy of r = 0.4 is observed only for collinear absorption and emission dipoles. At ambient temperatures, r ∼ 0 and r ∼ 0.2-0.3 for Ap free and in duplex DNA, respectively.
33. 10
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42. The yield of CT need not always decrease as the number of bridging bases increases (see ref 6b); we examined longer bridges to determine if certain bridges may inherently favor CT-active conformations.