Gonadotrophin-releasing hormone-I and-II stimulate steroidogenesis in prepubertal murine Leydig cells in vitro

Asian Journal of Andrology

Volumn 10, Issue 6, 2008, Pages 929-936

  Lin, Yung Ming ^a   Liu, Ming Yie ^a   Poon, Song Ling ^a   Leu, Sew Fen ^b   Huang, Bu Miin ^a  

^a NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

^b HUNGKUANG UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; GONADORELIN 1 AGONIST; GONADORELIN 2 AGONIST; GONADORELIN AGONIST; STEROID 17ALPHA MONOOXYGENASE; STEROIDOGENIC ACUTE REGULATORY PROTEIN; TESTOSTERONE; UNCLASSIFIED DRUG; CHOLESTEROL MONOOXYGENASE (SIDE CHAIN CLEAVING); GONADORELIN; PHOSPHOPROTEIN; RNA; STEROID;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG MECHANISM; ENZYME ACTIVATION; HORMONAL REGULATION; HORMONE ACTION; HORMONE SYNTHESIS; LEYDIG CELL; MOUSE; NONHUMAN; PREPUBERTY; PROTEIN EXPRESSION; REGULATORY MECHANISM; STEROIDOGENESIS; ANIMAL; BIOSYNTHESIS; C57BL MOUSE; CELL CULTURE; CELL SEPARATION; DOSE RESPONSE; DRUG EFFECT; DRUG POTENTIATION; GENETICS; ISOLATION AND PURIFICATION; MALE; METABOLISM; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEXUAL MATURATION; WESTERN BLOTTING;

3-HYDROXYSTEROID DEHYDROGENASES; ANIMALS; BLOTTING, WESTERN; CELL SEPARATION; CELLS, CULTURED; CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME; DOSE-RESPONSE RELATIONSHIP, DRUG; GONADOTROPIN-RELEASING HORMONE; LEYDIG CELLS; MALE; MICE; MICE, INBRED C57BL; PHOSPHOPROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; SEXUAL MATURATION; STEROIDS; TESTOSTERONE;

EID: 59449089120     PISSN: 1008682X     EISSN: 17457262     Source Type: Journal    
DOI: 10.1111/j.1745-7262.2008.00434.x     Document Type: Article

References (31)

1. Cheng CK, Leung PC. Molecular biology of gonadotropinsreleasing hormone (GnRH)-I, GnRH-II and their receptors in humans. Endocr Rev 2005; 26: 283-306.
2. Pierantoni R, Cobellis G, Meccariello R, Fasano S. Evolutionary aspects of cellular communication in the vertebrate hypothalamo-hypophysio-gonadal axis. Int Rev Cytol 2002; 218: 69-41.
3. Bahk JY, Hyun JS, Chung SH, Lee H, Kim MO, Lee BH, et al. Stage specific identification of the expression of GnRH mRNA and localization of the GnRH receptor in mature rat and adult human testis. J Urol 1995; 154: 1958-61.
4. Paull WK, Turkelson CM, Thomas CR, Arimura A. Immunohistochemical demonstration of a testicular substance related to luteinizing hormone-releasing hormone. Science 1981; 213: 1263-4.
5. Ramakrishnappa N, Rajamahendran R, Lin YM, Leung PC. GnRH in non-hypothalamic reproductive tissues. Anim Reprod Sci 2005; 88: 95-113.
6. Bourne GA, Regiani S, Payne AH, Marshall JC. Testicular GnRH receptors: characterization and localization on interstitial tissue. J Clin Endocr Metab 1980; 51: 407-9.
7. Clayton RN, Katikineni M, Chan V, Dufau ML, Catt KJ. Direct inhibition of testicular function by gonadotropin-releasing hormone: Mediation by specific gonadotropin-releasing hormone receptors in interstitial cell. P Natl Acad Sci USA 1980; 77: 4459-63.
8. Khan MA, Ferro VA, Stimson WH. Use of a highly specific monoclonal antibody against the central variable amino acid sequence of mammalian gonadotropin releasing hormone to evaluate GnRH-I tissue distribution compared with GnRH-I binding sites in adult male rats. Am J Reprod Immunol 2003; 49: 239-48.
9. Molcho J, Zakut H, Naor Z. Stimulation of prostaglandin E and testosterone production in rat interstitial cells by a gonadotropin-releasing hormone agonist. Endocrinology 1984; 114: 2382-7.
10. Browning JY, D'Agata R, Steinberger A, Grotjan Jr. HE, Steinberger E. Biphasic effect of gonadotropin-releasing hormone and its agonist analog (HOE766) on in vitro testosterone production by purified rat Leydig cells. Endocrinology 1983; 113: 985-91.
11. Kerr JB, Sharpe RW. Effects and interactions of LH and LHRH agonists on testicular morphology and function in hypophy-sectomized rats. J Reprod Fertil 1986; 76: 175-92.
12. Habert R. Effect of decapitation and chronic in-vivo treatment with a gonadotrophin-releasing hormone agonist on testicular steroidogenesis in the rat fetus. J Endocrinol 1992; 133: 245-51.
13. Gobbetti A, Zerani M. Mammalian GnRH involvement in prostaglandin F2 alpha and sex steroid hormones testicular release in two amphibian species: The anuran water frog, Rana esculenta, and the urodele crested newt, Triturus carnifex. Gen Comp Endocrinol 1992; 87: 240-8.
14. Wang NG, Sundaram K, Pavlou S, Rivier J, Vale W, Bardin CW. Mice are insensitive to the antitesticular effects of luteinizing hormone-releasing hormone agonists. Endocrinology 1983; 112: 331-5.
15. Huang BM, Stocco DM, Norman RL. The cellular mechanisms of corticotropin-releasing hormone (CRH)-stimulated steroidogenesis in mouse Leydig cells are similar to those for LH. J Androl 1997; 18: 528-34.
16. Poon SL, Leu SF, Hsu HK, Liu MY, Huang BM. Regulatory mechanism of Toona sinensis on mouse Leydig cell steroidogenesis. Life Sci 2005; 76: 1473-87.
17. Lowry OH, Rosenborough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol regent. J Biol Chem 1951; 193: 265-75.
18. Chen YJ, Huang YL, Huang BM. Cordyceps sinensis mycelium activates PKA and PKC signal pathways to stimulate steroidogenesis in MA-10 mouse Leydig tumor cells. Int J Biochem Cell Biol 2005; 37: 214-23.
19. Stocco DM, Clark BJ. Regulation of the acute production of steroids in steroidogenic cells. Endocr Rev 1996; 17: 221-44.
20. Guo IC, Wu LS, Lin JH, Chung BC. Differential inhibition of progesterone synthesis in bovine luteal cells by estrogens and androgens. Life Sci 2001; 68: 1851-65.
21. Ge RS, Dong Q, Sottas CM, Chen H, Zirkin BR, Hardy MP. Gene expression in rat Leydig cells during development from the progenitor to adult stage: A cluster analysis. Biol Reprod 2005; 72: 1405-15.
22. Chen L, Xin ZC, Li X, Tian L, Yuan YM, Liu G, et al. Cox7a2 mediates steroidogenesis in TM3 mouse Leydig cells. Asian J Androl 2006; 8: 589-94.
23. Sultana T, Svechnikov KV, Gustafsson K, Wahlgren A, Tham E, Weber G, et al. Molecular identity, expression and functional analysis of interleukin-1alpha and its isoforms in rat testis. Asian J Androl 2004; 6: 149-53.
24. Saez JM. Leydig cells: Endocrine, paracrine, and autocrine regulation. Endocr Rev 1994; 15: 574-626.
25. Payne AH, Hales DB. Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones. Endocr Rev 2004; 25: 947-70.
26. Payne AH, Youngblood GL. Regulation of expression of steroidogenic enzymes in Leydig cells. Biol Reprod 1995; 52: 217-25.
27. Reddy PR. Hormonal contraception for human males: Prospects. Asian J Androl 2000; 2: 46-50.
28. Calkins JH, Sigel MM, Nankin HR, Lin T. Interleukin-1 inhibits Leydig cell steroidogenesis in primary culture. Endocrinology 1988; 123: 1605-10.
29. Lin T, Haskell J, Vinson N, Terracio L. Direct stimulatory effects of insulin-like growth factor-I on Leydig cell steroidogenesis in primary culture. Biochem. Bioph Res Co 1986; 137: 950-6.
30. Lin T, Blaisdell J, Haskell JF. Transforming growth factor-beta inhibits Leydig cell steroidogenesis in primary culture. Biochem. Bioph Res Co 1987; 146: 387-94.
31. Lin T, Calkins JK, Morris PL, Vale W, Bardin CW. Regulation of Leydig cell function in primary culture by inhibin and activin. Endocrinology 1989; 125: 2134-40.