Use of a highly specific monoclonal antibody against the central variable amino acid sequence of mammalian gonadotropin releasing hormone to evaluate GnRH-I tissue distribution compared with GnRH-I binding sites in adult male rats

American Journal of Reproductive Immunology

Volumn 49, Issue 4, 2003, Pages 239-248

  Khan, Mohammad A H ^a   Ferro, Valerie A ^a   Stimson, William H ^a  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

Author keywords

GnRH I tissue distribution; Immunohistochemistry; Monoclonal antibodies; Variable and core region sequences

Indexed keywords

AMINO ACID; AVIDIN; BIOTIN; BOVINE SERUM ALBUMIN; FORMALDEHYDE; GONADORELIN; GONADORELIN I; HORSERADISH PEROXIDASE; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 1D71C10; MONOCLONAL ANTIBODY 4F22B6; MONOCLONAL ANTIBODY 7B101D10; NEUROPEPTIDE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ANTIBODY SPECIFICITY; ARTICLE; BINDING AFFINITY; BINDING SITE; BIOTINYLATION; CEREBELLUM; COMPARATIVE STUDY; CONTROLLED STUDY; DENDRITIC CELL; ENDOCARDIUM; ENDOCRINE FUNCTION; EPICARDIUM; FOREBRAIN; HEART MUSCLE; HORMONE BINDING; HORMONE DETERMINATION; HYPOPHYSIS; HYPOTHALAMUS; IMMUNOHISTOCHEMISTRY; KUPFFER CELL; LEYDIG CELL; LIVER CELL; MACROPHAGE; MALE; NERVE CELL; NEUTROPHIL; NONHUMAN; OLFACTORY NERVE; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; RAT; RAT STRAIN; SERTOLI CELL; SPERMATOZOON; SPLEEN LYMPHOCYTE; STAINING; T LYMPHOCYTE; TISSUE DISTRIBUTION; TISSUE FIXATION;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BINDING SITES; ENZYME-LINKED IMMUNOSORBENT ASSAY; GONADOTROPIN-RELEASING HORMONE; IMMUNOHISTOCHEMISTRY; MALE; PROTEIN ISOFORMS; RATS; RECEPTORS, LHRH;

EID: 0037951449     PISSN: 87558920     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0897.2003.01202.x     Document Type: Article

References (43)

1. Matsuo H, Baba Y, Nair RMG, Arimura A, Schally AV: Structure of porcine LH and FSH releasing hormone. I. The proposed amino acids sequenced. Biochem Biophys Res Commun 1971; 43:1334-1339.
2. Azad N, Agrawal L, Emanuele MA, Kelley MR, Mohagheghpour N, Lawrence AM, Emanuele NV: Neuroimmunoendocrinology. Am J Reprod Immunol 1991; 26:160-172.
3. Varma S, Sabharwal P, Malarkey WB: Human splenocytes secrete LHRH, which inhibit lymphocytes proliferation. Prog Neuro Endocrine Immunol 1992; 5:187-191.
4. Seeburg PH, Mason AJ, Stewart JA, Nikolics K: The mammalian GnRH gene and its pivotal role in reproduction. Recent Prog Horm Res 1987; 43:69-98.
5. Kakar SS, Jennes L: Expression of gonadotropin releasing hormone and gonadotropin releasing hormone receptor mRNAs in various non-reproductive tissues. Cancer Lett 1995; 98:57-62.
6. Leung PCK, Steel GL: Intracellular signaling in the gonads. Endocrinol Rev 1992; 13:467-498.
7. Urbanski HF: Monoclonal antibodies to luteinizing hormone-releasing hormone: Production, characterization, and immunocytochemical application. Biol Reprod 1991; 44:681-686.
8. Chen HF, Jeung EB, Stephenson M, Leung PC: Human peripheral blood mononuclear cells express gonadotropin releasing hormone (GnRH), GnRH receptor and interleukin-2 receptor gamma-chain messenger ribonucleic acids that are regulated by GnRH in vitro. J Clin Endocrinol Metab 1999; 84:743-750.
9. Clayton RN, Harwood JP, Catt KJ: Gonadotropin releasing hormone analogue binds to luteal cells and inhibit progesterone production. Nature 1989; 282:90-92.
10. Flanagan CA, Becker II, Davidson JS, Wakefield IK, Zhou W, Sealfon SC, Miller RP: Glutamate 301 of the mouse gonadotropin releasing hormone receptor confers specificity for arginine 8 of mammalian gonadotropin releasing hormone. J Biol Chem 1994; 269:22636-22641.
11. Fan NC, Jeung E, Peng C, Olofsson JI, Krisinger J, Leung PCK: The human gonadotropin releasing hormone (GnRH) receptor gene, cloning, genomic organisation and chromosomal assignment. Mol Cell Endocrinol 1994; 103:R1-R6.
12. Hsueh AJW, Jones PBC: Extrapituitary action of gonadotropin releasing hormone. Endocrine Rev 1981; 2:437-461.
13. Kakar SS, Grantham K, Musgrove LC, Devor D, Sellers JC, Neill JD: Rat gonadotropin releasing hormone (GnRH) receptor: Tissue expression and hormonal regulation of its mRNA. Mol Cell Endocrinol 1994; 101:151-157.
14. Imai A, Ohno T, Lida K, Fuseya T, Furui T, Tamaya T: Gonadotropin releasing hormone receptor in gynecologic tumors. Frequent expression in adenocarcinoma histologic types. Cancer 1994; 74:2555-2561.
15. Botte MC, Chamagne AM, Carre MC, Counis R, Kottler ML: Fetal expression of GnRH and GnRH receptor genes in rat testes and ovary. J Endocrinol 1998; 159:179-189.
16. Lee C-YG, Ho J, Chow S-N, Yasojima K, Schwab C, McGeer PL: Immunoidentification of gonadotropin releasing hormone receptor in human sperm, pituitary and cancer cells. Am J Reprod Immunol 2000; 44:170-177.
17. Nett TM, Akber AM, Niswender GD: Serum levels of luteinising hormone and gonadotropin releasing hormone in cycling, castrated and anoestrous ewes. Endocrinol 1974; 94:713-718.
18. White RB, Eisen JA, Kasten TL, Fernald RD: Second gene for gonadotropin releasing hormone in humans. Proc Natl Acad Sci U S A 1998; 95:305-309.
19. Yu WH, Kananth S, Walczewska A, Sower SA, McCann SM: A hypothalamic follicle-stimulating hormone-releasing decapeptide in the rat. Proc Natl Acad Sci U S A 1997; 94:9499-9503.
20. Yahalom D, Chen A, Ben-Aroya N, Rahimipour S, Kaganovsky E, Okon E, Fridkin M, Koch Y: The gonadotropin releasing hormone family of neuropeptides in the brain of human, bovine and rat: Identification of a third isoform. FEBS Lett 1999; 463:289-294.
21. White SA, Kasten TL, Bond CT, Adelman JP, Fernald RD: Three gonadotropin hormone genes in one organism suggest novel rules for an ancient peptide. Proc Natl Acad Sci U S A 1995; 92:8363-8367.
22. Urbanski HF, Richard BW, Russel DF, Kohama SG, Garyfallou VT, Densmore VS: Regional expression of mRNA encoding a second form of gonadotropin-releasing hormone in the Macaque brain. Endocrinol 1999; 140:1945-1948.
23. Inaba T, Shimada S, Gonda M, Mori J, Tokunaga T, Geshi M, Torris R: Enzyme immunoassay of gonadotropin releasing hormone in the canine hypothalamus and plasma using monoclonal antibodies. Br Vet J 1994; 150:85-92.
24. Ferro VA, Khan MAH, Latimer VS, Brown D, Urbanski HF, Stimson WH: Immunoneutralisation of GnRH-I, without cross-reactivity to GnRH-II, in the development of a highly specific antifertility vaccine for clinical and veterinary use. Am J Reprod Immunol 2001; 51:109-129.
25. Ferro VA, O'Grady JE, Notman J, Stimson WH: Immunological castration using a gonadotropin-releasing hormone analogue to PPD. Food Agric Immunol 1995; 7:259-272.
26. Rahman SR, Stimson WH: Characterization of monoclonal antibodies with specificity for the core oligosaccharide of Shigella lipopolysaccharide. Hybridoma 2001; 20:85-90.
27. Saavedra R, Joseph Braveo P, Charli JL, Herion P: Characterisation of high affinity monoclonal antibodies against the luteinising hormone releasing hormone. Hybridoma 1987; 6:663-672.
28. Li Q, Alston-Mills B, Ottinger MA: Avian LHRH during embryonic development: Measurement by competitive ELISA with a monoclonal antibody. General Comp Endocrinol 1991; 82:444-450.
29. Park MK, Wakabayashi K: Preparation of monoclonal antibody to common amino acid sequence of LHRH and its application. Endocrinol Japan 1986; 33:257-272.
30. Alston-Mills B, Li Q, Ottinger MA: Production of monoclonal antibodies against avian LHRH-I. In Vitro Cell Dev Biol 1989; 25:934-938.
31. Belda M, Covenas R, Narvaez JA, Aguirre JA, Tramu G: Distribution of luteinising hormone releasing hormone in the upper brain stem and diencephalon of the cat: An immunocytochemical study. Brain Res Bull 2000; 51:281-291.
32. Karuri AR, Ayres S, Kumar MSA: Regional distribution of gonadotropin-releasing hormone-like, β-endorphin-like, and methionine-enkephalin-like immunoreactivities in the central nervous system of the goat. Brain Res Bull 2000; 51:63-68.
33. Byrne B, Klahn S, Taylor PL, Eidne KA: Functional analysis of GnRH receptor ligand binding using biotinylated GnRH derivatives. Mol Cell Endocrinol 1998; 144:11-19.
34. Sealfon SC, Weinstein H, Millar RP: Molecular mechanisms of ligand interaction with the gonadotropin releasing hormone receptor. Endocr Rev 1997; 18:180-205.
35. Clayton RN, Katikineni M, Chan V, Dufau ML, Catt KJ: Direct inhibition of testicular function by gonadotropin releasing hormone: Mediation by specific gonadotrophin releasing hormone receptor in interstitial cells. Proc Natl Acad Sci U S A 1980; 77:4459-4463.
36. Jennes L, Eyigor O, Janovik JA, Conn PM: Brain gonadotropin releasing hormone receptors. Localization Regulation Recent Prog Horm Res 1997; 52:475-490.
37. Dellovade T, Schwanzel-Fukuda M, Gordan IJ, Pfaff D: Aspects of GnRH neurobiology conserved across vertebrate forms. General Comparative Endocrinol 1998; 112:276-282.
38. Millar R, Lowe S, Conklin D, Pawson A, Maudsley S, Troskie B, Ott T, Millar M, Lincoln G, Sellar R, Faurholm B, Scobie G, Keustner R, Terasawa E, Katz A. A novel mammalian receptor for the evolutionarily conserved type II GnRH. Cell biology. Proc Natl Acad Sci U S A 2001; 98:9636-9641.
39. Tortora GJ, Anagnostakos NP: The brain and cranial nerves. In Principles of Anatomy and Physiology, Dollinger EA, Ginsberg R, Forrell TR (eds). New York, Harper & Row Publisher, 1990, pp. 384-423.
40. Wirsing-Wiechmann CR: Function of gonadotropin releasing hormone in olfaction. Keio J Med 2001; 50:81-85.
41. Handelsman DJ, Swerdloff RS: Pharmacokinetics of gonadotropin-releasing hormone and its analogues. Endocr Rev 1986; 7:95-105.
42. Conn PM, Crowly WF: Gonadotropin-releasing hormone and its analogs. Annu Rev Med 1994; 45: 391-405.
43. Iwashita M, Evans M, Catt KJ: Characterisation of gonadotropin releasing hormone receptor site in term placenta and chorionic villi. J Clin Endocrinol Metab 1986; 62:127-133.