The splicing factor Prp17 interacts with the U2, U5 and U6 snRNPs and associates with the spliceosome pre- and post-catalysis

Biochemical Journal

Volumn 416, Issue 3, 2008, Pages 365-374

  Sapra, Aparna K ^a   Khandelia, Piyush ^a   Vijayraghavan, Usha ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

pre mRNA splicing; Prp17; Saccharomyces cerevisiae; Second step splicing; Spliceosome

Indexed keywords

PRE-MRNA SPLICING; PRP17; SACCHAROMYCES CEREVISIAE; SECOND-STEP SPLICING; SPLICEOSOME;

CATALYSIS;

ASSOCIATION REACTIONS;

ACTIN; FUNGAL PROTEIN; HELICASE; MESSENGER RNA PRECURSOR; PROTEIN PRP16; PROTEIN PRP17; PROTEIN PRP19; PROTEIN SNU114; PROTEIN U2; PROTEIN U5; PROTEIN U6; SMALL NUCLEAR RIBONUCLEOPROTEIN; UNCLASSIFIED DRUG; CDC40 PROTEIN, S CEREVISIAE; CELL CYCLE PROTEIN; DNA BINDING PROTEIN; PRP8 PROTEIN, S CEREVISIAE; RNA BINDING PROTEIN; RNA PRECURSOR; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; IMMUNOPRECIPITATION; INTRON; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN INTERACTION; PROTEIN RNA BINDING; RNA SPLICING; SACCHAROMYCES CEREVISIAE; SPLICEOSOME; TEMPERATURE; GENETICS; METABOLISM;

SACCHAROMYCES CEREVISIAE;

CATALYSIS; CELL CYCLE PROTEINS; DNA-BINDING PROTEINS; RIBONUCLEOPROTEIN, U2 SMALL NUCLEAR; RIBONUCLEOPROTEIN, U4-U6 SMALL NUCLEAR; RIBONUCLEOPROTEIN, U5 SMALL NUCLEAR; RNA PRECURSORS; RNA SPLICING; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SPLICEOSOMES;

EID: 58949099708     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20081195     Document Type: Article

References (46)

1. Brow, D. A. (2002) Allosteric cascade of spliceosome activation. Annu. Rev. Genet. 36, 333-360
2. Jurica, M. S. and Moore, M. J. (2003) Pre-mRNA splicing: awash in a sea of proteins. Mol. Cell 12, 5-14
3. Schwer, B. and Guthrie, C. (1991) PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome. Nature 349, 494-499
4. Schwer, B. and Guthrie, C. (1992) A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis. EMBO J. 11, 5033-5039
5. Vijayraghavan, U., Company, M. and Abelson, J. (1989) Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev. 3, 1206-1216
6. Vijayraghavan, U. and Abelson, J. (1990) PRP18, a protein required for the second reaction in pre-mRNA splicing. Mol. Cell. Biol. 10, 324-332
7. Frank, D., Patterson, B. and Guthrie, C. (1992) Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing. Mol. Cell. Biol. 12, 5197-5205
8. Umen, J. G. and Guthrie, C. (1995) A novel role for a U5 snRNP protein in 3′ splice-site selection. Genes Dev. 9, 855-868
9. Schwer, B. and Gross, C. H. (1998) Prp22, a DExH box helicase, plays two distinct roles in yeast pre-mRNA splicing. EMBO J. 17, 2086-2094
10. Lossky, M., Anderson, G. J., Jackson, S. P. and Beggs, J. D. (1987) Identification of a yeast snRNP protein and detection of snRNP-snRNP interactions. Cell 51, 1019-1026
11. Whittaker, E., Lossky, M. and Beggs, J. D. (1990) Affinity purification of spliceosomes reveals that the precursor RNA processing protein PRP8, a protein in the U5 small nuclear ribonucleoprotein particle, is a component of yeast spliceosomes. Proc. Natl. Acad. Sci. U.S.A. 87, 2216-2219
12. Ansari, A. and Schwer, B. (1995) SLU7 and a novel activity, SSF1, act during the PRP16dependent step of yeast pre-mRNA splicing. EMBO J. 14, 4001-4009
13. Jones, M. H., Frank, D. N. and Guthrie, C. (1995) Characterization and functional ordering of Slu7p and Prp17p during the second step of pre-mRNA splicing in yeast. Proc. Natl. Acad. Sci. U.S.A. 92, 9687-9691
14. Umen, J. G. and Guthrie, C. (1995) Prp16p, Slu7p, and Prp8p interact with the 3′ splice-site in two distinct stages during the second catalytic step of pre-mRNA splicing. RNA 1, 584-597
15. McPheeters, D. S. and Muhlenkamp, P. (2000) Spatial organization of protein-RNA interactions in the branch site-3′ splice-site region during pre-mRNA splicing in yeast. Mol. Cell. Biol. 23, 4174-4186
16. O'Keefe, R. T., Norman, C. and Newman, A. J. (1996) The invariant U5 snRNA loop I sequence is dispensable for the first catalytic step of pre-mRNA splicing in yeast. Cell 86, 679-689
17. Stevens, S. W. (2000) Analysis of low-abundance ribonucleoprotein particles from yeast by affinity chromatography and mass spectrometry microsequencing. Methods Enzymol. 318, 385-398
18. Newman, A. J., Lin, R. J., Cheng, S. C. and Abelson, J. (1985) Molecular consequences of specific intron mutations in yeast mRNA splicing in vivo and in vitro. Cell 68, 743-754
19. Vijayraghavan, U., Parker, R., Tamm, J., Iimura, Y., Rossi, J., Abelson, J. and Guthrie, C. (1986) Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome. EMBO J. 5, 1683-1695
20. Tsai, W. Y., Chow, Y. T., Chen, H. R., Huang, K. T., Hong, R. I., Jan, S. P., Kuo, N. Y., Tsao, T. Y., Chen, C. H. and Cheng, S. C. (1999) Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing. J. Biol. Chem. 274, 9455-9462
21. Cheng, S. C. and Abelson, J. (1987) Spliceosome assembly in yeast. Genes Dev. 1, 1014-1027
22. Tarn, W. Y., Lee, K. R. and Cheng, S. C. (1993) Yeast precursor mRNA processing protein PRP19 associates with the spliceosome concomitant with or just after dissociation of U4 small nuclear RNA. Proc. Natl. Acad. Sci. U.S.A. 90, 10821-10825
23. McPheeters, D. S., Fabrizio, P. and Abelson, J. (1989) In vitro reconstitution of functional yeast U2 snRNPs. Genes Dev. 3, 2124-2136
24. Fabrizio, P., McPheeters, D. S. and Abelson, J. (1989) In vitro assembly of yeast U6 snRNP, a functional assay. Genes Dev. 3, 2137-2150
25. Horowitz, D. S. and Abelson, J. (1993) A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae. Mol. Cell. Biol. 13, 2959-2970
26. Horowitz, D. S. and Abelson, J. (1993) Stages in the second reaction of pre-mRNA splicing, the final step is ATP independent. Genes Dev. 7, 320-329
27. Lindsey, L. A. and Garcia-Blanco, M. A. (1998) Functional conservation of the human homolog of the yeast pre-mRNA splicing factor Prp17p. J. Biol. Chem. 273, 32771-32775
28. Seshadri, V., Vaidya, V. C. and Vijayraghavan, U. (1996) Genetic studies of the PRP17 gene of Saccharomyces cerevisiae, a domain essential for function maps to a non-conserved region of the protein. Genetics 143, 45-55
29. Lindsey-Boltz, L. A., Chawla, G., Srinivasan, N., Vijayraghavan, U. and Garcia-Blanco, M. A. (2000) The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function. RNA 6, 1289-1305
30. Chawla, G., Sapra, A. K., Surana, U. and Vijayraghavan, U. (2003) Dependence of pre-mRNA introns on PRP17, a non-essential splicing factor, implications for efficient progression through cell cycle transitions. Nucleic Acids Res. 31, 2333-2343
31. Brys, A. and Schwer, B. (1996) Requirement for Slu7 in yeast pre-mRNA splicing is dictated by the distance between the branch point and the 3′ splice-site. RNA 2, 707-717
32. Ben-Yehuda, S., Dix, I., Russell, C. S., McGarvey, M., Beggs, J. D. and Kupiec, M. (2000) Genetic and physical interactions between factors involved in both cell-cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae. Genetics 156, 1503-1517
33. Chen, H. R., Jan, S. P., Tsao, T. Y., Sheu, Y. J., Banroques, J. and Cheng, S. C. (1998) Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p. Mol. Cell. Biol. 18, 2196-2204
34. Chen, C. H., Yu, W. C., Tsao, T. Y., Wang, L. Y., Chen, H. R., Lin, J. Y., Tsai, W. Y. and Cheng, S. C. (2002) Functional and physical interaction between components of the Prp19p-associated complex. Nucleic Acids Res. 30, 1029-1037
35. Chan, S. P., Kao, D. I., Tsai, W. Y. and Cheng, S. C. (2003) The Prp19p-associated complex in spliceosome activation. Science 302, 279-282
36. Sapra, A. K., Arava, Y., Khandelia, P. and Vijayraghavan, U. (2004) Genome-wide analysis of pre-mRNA splicing, intron features govern the requirement for the second-step factor, Prp17 in Saccharomyces cerevisiae and Schizosaccharomyces pombe. J. Biol. Chem. 279, 52437-52446
37. Bacikova, D. and Horowitz, D. S. (2002) Mutational analysis identifies two separable roles of the Saccharomyces cerevisiae splicing factor Prp18. RNA 8, 1280-1293
38. Gavin, A. C., Bosche, M., Krause, R., Grandi, P., Marzioch, M., Bauer, A., Schultz, J., Rick, J. M., Michon, A. M., Cruciat, C. M. et al. (2002) Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415, 141-147
39. Ohi, M. D., Link, A. J., Ren, L., Jennings, J. L., McDonald, W. H. and Gould, K. L. (2002) Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol. Cell. Biol. 22, 2011-2024
40. Dix, I., Russell, C., Ben-Yehuda, S., Kupiec, M. and Beggs, J. D. (1999) The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae. RNA 5, 360-368
41. Russell, C. S., Ben-Yehuda, S., Dix, I., Kupiec, M. and Beggs, J. D. (2000) Functional analyses of interacting factors involved in both pre-mRNA splicing and cell-cycle progression in Saccharomyces cerevisiae. RNA 6, 1565-1572
42. Xu, D., Field, D. J., Tang, S. J., Moris, A., Bobechko, B. P. and Friesen, J. D. (1998) Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing. Mol. Cell. Biol. 18, 2055-2066
43. Jurica, M. S., Licklider, L. J., Gygi, S. P., Grigorieff, N. and Moore, M. J. (2002) Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA 8, 426-439
44. Chung, S., McLean, M. R. and Rymond, B. C. (1999) Yeast ortholog of the Drosophila crooked neck protein promotes spliceosome assembly through stable U4/U6.U5 snRNP addition. RNA 5, 1042-1054
45. Makarov, E. M., Makarova, O. V., Urlaub, H., Gentzel, M., Will, C. L., Wilm, M. and Luhrmann, R. (2002) Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome. Science 298, 2205-2208
46. Makarova, O. V., Makarov, E. M., Urlaub, H., Will, C. L., Gentzel, M., Wilm, M. and Luhrmann, R. (2004) A subset of human 35S U5 proteins, including Prp19, function prior to catalytic step 1 of splicing. EMBO J. 23, 2381-2391