The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of pre-mRNA splicing in yeast

Cell

Volumn 86, Issue 4, 1996, Pages 679-689

  O'Keefe, Raymond T ^a   Norman, Christine ^a   Newman, Andrew J ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CONTROLLED STUDY; EXON; FUNGAL GENETICS; GENE DELETION; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; RNA ANALYSIS; RNA SPLICING; YEAST;

EID: 0030598839     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(00)80140-3     Document Type: Article

References (46)

1. Boeke, J.D., LaCroute, F., and Fink, G.R. (1984). A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet. 197, 345-346.
2. Cech, T.R. (1986). The generality of self-splicing RNA: relationship to nuclear mRNA splicing. Cell 44, 207-210.
3. Cortes, J.J., Sontheimer, E.J., Seiwert, S.D., and Steitz, J.A. (1993). Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5′ splice sites in vivo. EMBO J. 12, 5181-5189.
4. Fabrizio, P., McPheeters, D.S., and Abelson, J. (1989). In vitro assembly of yeast U6 snRNP: a functional assay. Genes Dev. 3, 2137-2150.
5. Frank, D.N., Roiha, H., and Guthrie, C. (1994). Architecture of the U5 small nuclear RNA. Mol. Cell. Biol. 14, 2180-2190.
6. Heus, H.A., and Pardi, A. (1991). Structural features that give rise to the unusual stability of RNA hairpins containing GNRA loops. Science 253, 191-194.
7. Jacquier, A., and Jacquesson-Breuleux, N. (1991). Splice site selection and role of the lariat in a group II intron. J. Mol. Biol. 219, 415-428.
8. Jacquier, A., and Michel, F. (1987). Multiple exon-binding sites in class II self-splicing introns. Cell 50, 17-29.
9. Jarmolowski, A., and Mattaj, I.W. (1993). The determinants for Sm protein binding to Xenopus U1 and U5 snRNAs are complex and non-identical. EMBO J. 12, 223-232.
10. Jones, M.H., and Guthrie, C. (1990). Unexpected flexibility in an evolutionary conserved protein-RNA interaction: genetic analysis of the Sm binding site. EMBO J. 9, 2555-2561.
11. Kandels-Lewis, S., and Séraphin, B. (1993). Role of U6 snRNA in 5′ splice site selection. Science 262, 2035-2039.
12. Konforti, B.B., Koziolkiewicz, M.J., and Konarska, M.M. (1993). Disruption of base pairing between the 5′ splice site and the 5′ end of U1 snRNA is required for spliceosome assembly. Cell 75, 863-873.
13. Lamm, G.M., Blencowe, B.J., Sproat, B.S., Iribarren, A.M., Ryder, U., and Lamond, A.I. (1991). Antisense probes containing 2-amino-adenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts. Nucleic Acids Res. 19, 3193-3198.
14. Lesser, C.A., and Guthrie, C. (1993). Mutations in U6 snRNA that alter splice site specificity: implications for the active site. Science 262, 1982-1988.
15. Madhani, H.D., and Guthrie, C. (1992). A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome. Cell 71, 803-817.
16. Madhani, H.D., and Guthrie, C. (1994). Dynamic RNA-RNA interactions in the spliceosome. Annu. Rev. Genet. 28, 1-26.
17. McPheeters, D.S., Fabrizio, P., and Abelson, J. (1989). In vitro reconstitution of functional yeast U2 snRNPs. Genes Dev. 3, 2124-2136.
18. Michel, F., and Ferat, J.-L. (1995). Structure and activities of group II introns. Annu. Rev. Biochem. 64, 435-461.
19. Moore, M.J., Query, C.C., and Sharp, P.A. (1993). Splicing of precursors to mRNA by the spliceosome. In The RNA World, R.F. Gesteland, and J.F. Atkins, eds. (Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press), pp. 303-357.
20. Newman, A., and Norman, C. (1991). Mutations in yeast U5 snRNA alter the specificity of 5′ splice-site cleavage. Cell 65, 115-123.
21. Newman, A.J., and Norman, C. (1992). U5 snRNA interacts with exon sequences at 5′ and 3′ splice sites. Cell 68, 743-754.
22. Newman, A.J., Lin, R.-J., Cheng, S.-C., and Abelson, J. (1985). Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitro. Cell 42, 335-344.
23. Newman, A.J., Teigelkamp, S., and Beggs, J.D. (1995). snRNA interactions at 5′ and 3′ splice sites monitored by photoactivated cross-linking in yeast spliceosomes. RNA 1, 968-980.
24. Patterson, B., and Guthrie, C. (1987). An essential yeast snRNA with a U5-like domain is required for splicing in vivo. Cell 49, 613-624.
25. Query, C.C., Moore, M.J., and Sharp, P.A. (1994). Branch nucleophile selection in pre-mRNA splicing: evidence for the bulged duplex model. Genes Dev. 8, 587-597.
26. Reyes, J.L., Kois, P., Konforti, B.B., and Konarska, M.M. (1996). The canonical GU dinucleotide at the 5′ splice site is recognized by p220 of the U5 snRNP within the spliceosome. RNA 2, 213-225.
27. Rymond, B.C., and Rosbash, M. (1992). Yeast pre-mRNA splicing. In The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, 2, J.R. Broach, J.R. Pringle, and E.W. Jones, eds. (Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press), pp. 143-192.
28. Sawa, H., and Abelson, J. (1992). Evidence for a base-pairing interaction between U6 small nuclear RNA and the 5′ splice site during the splicing reaction in yeast. Proc. Natl. Acad. Sci. USA 89, 11269-11273.
29. Sawa, H., and Shimura, Y. (1992). Association of U6 snRNA with the 5′ splice site region in the spliceosome. Genes Dev. 6, 244-254.
30. Ségault, V., Will, C.L., Sproat, B.S., and Lührmann, R. (1995). In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing: Sm proteins are functionally interchangeable and are essential for the formation of functional U2 and U5 snRNPs. EMBO J. 14, 4010-4021.
31. Séraphin, B., and Rosbash, M. (1990). Exon mutations uncouple 5′ splice site selection from U1 snRNA pairing. Cell 63, 619-629.
32. Séraphin, B., Kretzner, L., and Rosbash, M. (1988). A U1 snRNA-pre-mRNA base pairing interaction is required early in spliceosome assembly but does not uniquely define the 5′ splice site. EMBO J. 7, 2533-2538.
33. Séraphin, B., Abovich, N., and Rosbash, M. (1991). Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly. Nucleic Acids Res. 19, 3857-3860.
34. Sharp, P.A. (1991). Five easy pieces. Science 254, 663.
35. Sikorski, R.S., and Hieter, P. (1989). A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27.
36. Siliciano, P.G., and Guthrie, C. (1988). 5′ splice site selection in yeast: genetic alterations in base-pairing with U1 reveal additional requirements. Genes Dev. 2, 1258-1267.
37. Sontheimer, E.J., and Steitz, J.A. (1993). The U5 and U6 small nuclear RNAs as the active site components of the spliceosome. Science 262, 1989-1996.
38. Steitz, T.A., and Steitz, J.A. (1993). A general two-metal-ion mechanism for catalytic RNA. Proc. Natl. Acad. Sci. USA 90, 6498-6502.
39. Teigelkamp, S., Newman, A.J., and Beggs, J.D. (1995). Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggests a role in stabilization of exon alignment by U5 snRNA. EMBO J. 14, 2602-2612.
40. Umen, J.G., and Guthrie, C. (1995). The second catalytic step of pre-mRNA splicing. RNA 1, 869-885.
41. Varani, G., Cheong, C., and Tinoco, I. (1991). Structure of an unusually stable RNA hairpin. Biochemistry 30, 3280-3289.
42. Weiner, A.M. (1993). mRNA splicing and autocatalytic introns: distant cousins or the products of chemical determinism? Cell 72, 161-164.
43. Winkelmann, G., Bach, M., and Lührmann, R. (1989). Evidence from complementation assays in vitro that U5 snRNP is required for both steps of mRNA splicing. EMBO J. 8, 3105-3112.
44. Wise, J. (1993). Guides to the heart of the spliceosome. Science 262, 1978-1979.
45. Wyatt, J.R., Sontheimer, E.J., and Steitz, J.A. (1992). Site-specific cross-linking of mammalian U5 snRNP to the 5′ splice site before the first step of pre-mRNA splicing. Genes Dev. 6, 2542-2553.
46. Zhuang, Y., and Weiner, A.M. (1986). A compensatory base change in U1 snRNA supresses a 5′ splice site mutation. Cell 46, 827-835.