His22 of TLXI plays a critical role in the inhibition of glycoside hydrolase family 11 xylanases

Journal of Enzyme Inhibition and Medicinal Chemistry

Volumn 24, Issue 1, 2009, Pages 38-46

  Rombouts, Sigrid ^a   Fierens, Ellen ^b   Vandermarliere, Elien ^c   Voet, Arnout ^b   Gebruers, Kurt ^b   Beaugrand, Johnny ^b   Courtin, Christophe M ^b   Delcour, Jan A ^b   de Maeyer, Marc ^b   Rabijns, Anja ^c   Van Campenhout, Steven ^a   Volckaert, Guido ^a  

^a LABORATORY OF GENE TECHNOLOGY   (Belgium)

^b UNIVERSITY OF LEUVEN   (Belgium)

^c DEPARTMENT OF PHARMACEUTICAL AND PHARMACOLOGICAL SCIENCES   (Belgium)

Author keywords

Pichia pastoris; Protein protein interaction; Site directed mutagenesis; Thaumatin like protein; Xylanase inhibitor

Indexed keywords

GLYCOSIDASE; HISTIDINE; MUTANT PROTEIN; PROTEIN TLXI; THAUMATIN; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; ENZYME ANALYSIS; ENZYME INHIBITION; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS;

CLONING, MOLECULAR; ENDO-1,4-BETA XYLANASES; GLYCOSIDE HYDROLASES; HISTIDINE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PLANT PROTEINS; PROTEIN BINDING; TRITICUM;

PICHIA PASTORIS; TRITICUM AESTIVUM;

EID: 58949083824     PISSN: 14756366     EISSN: 14756374     Source Type: Journal    
DOI: 10.1080/14756360701841913     Document Type: Article

References (31)

1. Courtin, CM and Delcour, JA (1998) Physicochemical and bread-making properties of low molecular weight wheat derived arabinoxylans. J Agric Food Chem, 46, pp. 4066-4073.
2. Courtin, CM and Delcour, JA (2002) Arabinoxylans and endoxylanases in wheat flour bread-making. J Cereal Sci, 35, pp. 225-243.
3. Ingelbrecht, JA, Moers, K., Abecassis, J., Rouau, X. and Delcour, JA (2002) Influence of arabinoxylans and endoxylanases on pasta processing and quality. Production of high-quality pasta with increased levels of soluble fiber. Cereal Chem, 78, pp. 721-729.
4. Romanowska, I., Polak, J., Janowska, K. and Bielecki, S. (2003) The application of fungal endoxylanase in bread-making. Commun Agric Appl Biol Sci, 68, pp. 317-320.
5. Polizeli, ML, Rizzatti, AC, Monti, R., Terenzi, HF, Jorge, JA and Amorim, DS (2005) Xylanases from fungi: Properties and industrial applications. Appl Microbiol Biotechnol, 67, pp. 577-591.
6. Henrissat, B. (1991) A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J, 280, pp. 309-316.
7. Brito, N., Espino, JJ and Gonzalez, C. (2006) The endo-beta-1,4-xylanase Xyn11A is required for virulence in Botrytis cinerea. Mol Plant Microbe Interact, 19, pp. 25-32.
8. Simpson, DJ, Fincher, GB, Huang, AHC and Cameron-Mills, V. (2002) Structure and function of cereal and related higher plant (1 → 4)-β-xylan endohydrolases. J Cereal Sci, 37, pp. 111-127.
9. Debyser, W., Derdelinckx, G. and Delcour, JA (1997) Arabinoxylan solubilisation and inhibition of the barley malt xylanolytic system by wheat during brewing with wheat wholemeal adjunct: Evidence for a new class of enzyme inhibitors. J Am Soc Brew Chem, 55, pp. 153-156.
10. McLauchlan, WR, Garcia-Conesa, MT, Williamson, G., Roza, M., Ravestein, P. and Maat, J. (1999) A novel class of protein from wheat which inhibits xylanases. Biochem J, 338, pp. 441-446.
11. Fierens, E., Rombouts, S., Gebruers, K., Courtin, CM, Goesaert, H., Brijs, K., Beaugrand, J., Volckaert, G., Van Campenhout, S., Proost, P. and Delcour, JA (2007) TLXI, a novel type of xylanase inhibitor from wheat (Triticum aestivum) belonging to the thaumatin family. Biochem J, 403, pp. 583-591.
12. Sørensen, JF, Kragh, KM, Sibbesen, O., Delcour, J., Goesaert, H., Svensson, B., Tahir, TA, Brufau, J., Perez-Vendrell, AM, Bellincampi, D., D'Ovidio, R., Camardella, L., Giovane, A., Bonnin, E. and Juge, N. (2004) Potential role of glycosidase inhibitors in industrial biotechnological applications. Biochim Biophys Acta, 1696, pp. 275-287.
13. Henrissat, B. (1991) A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J, 280, pp. 309-316.
14. Sansen, S., De Ranter, CJ, Gebruers, K., Brijs, K., Courtin, CM, Delcour, JA and Rabijns, A. (2004) Structural basis for inhibition of Aspergillus niger xylanase by Triticum aestivum xylanase inhibitor-I. J Biol Chem, 279, pp. 36022-36028.
15. Fierens, K., Gils, A., Sansen, S., Brijs, K., Courtin, CM, Declerck, PJ, De Ranter, CJ, Gebruers, K., Rabijns, A., Robben, J., Campenhout, S., Volckaert, G. and Delcour, JA (2005) His374 of wheat endoxylanase inhibitor TAXI-I stabilizes complex formation with glycoside hydrolase family 11 endoxylanases. FEBS J, 272, pp. 5872-5882.
16. Payan, F., Leone, P., Porciero, S., Furniss, C., Tahir, T., Williamson, G., Durand, A., Manzanares, P., Gilbert, HJ, Juge, N. and Roussel, A. (2004) The dual nature of the wheat xylanase protein inhibitor XIP-I: Structural basis for the inhibition of family 10 and family 11 xylanases. J Biol Chem, 279, pp. 36029-36037.
17. Schimoler-O'Rourke, R., Richardson, M. and Selitrennikoff, CP (2001) Zeamatin inhibits trypsin and α-amylase activities. Appl Environ Microbiol, 67, pp. 2365-2366.
18. Smith, AM and Klugman, KP (1997) "Megaprimer" method of PCR-based mutagenesis: The concentration of megaprimer is a critical factor. Biotechniques, 22, pp. 438-442.
19. Shi, X., Karkut, T., Chamankhah, M., Alting-Mees, M., Hemmingsen, SM and Hegedus, D. (2003) Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris. Protein Expr Purif, 28, pp. 321-330.
20. Beaugrand, J., Gebruers, K., Ververken, C., Fierens, E., Croes, E., Goddeeris, B., Courtin, CM and Delcour, JA (2006) Antibodies against wheat xylanase inhibitors as tools for the selective identification of their homologues in other cereals. J Cereal Sci, 44, pp. 59-67.
21. Fierens, K., Geudens, N., Brijs, K., Courtin, CM, Gebruers, K., Robben, J., Van Campenhout, S., Volckaert, G. and Delcour, JA (2004) High-level expression, purification and characterization of recombinant wheat xylanase inhibitor TAXI-I secreted by the yeast Pichia pastoris. Protein Expr Purif, 37, pp. 39-46.
22. Ko, TP, Day, J., Greenwood, A. and Mcpherson, A. (1994) Structures of three crystal forms of the sweet protein thaumatin. Acta Crystallog D, 50, pp. 813-825.
23. McGuffin, LJ and Jones, DT (2003) Improvement of the GenTHREADER method for genomic fold recognition. Bioinformatics, 19, pp. 874-881.
24. Laskowski, RA, McArthur, MW, Moss, DS and Thornton, JM (1993) PROCHECK: A program to check the stereochemical quality of protein structures. J Appl Cryst, 26, pp. 283-291.
25. Bond, CS (2003) TopDraw: A sketchpad for protein structure topology cartoons. Bioinformatics, 19, pp. 311-312.
26. Gebruers, K. (2002) Endoxylanase inhibitors in wheat (Triticum aestivum L.): Isolation, characterisation and use for endoxylanase purification [dissertation].p. 178. Katholieke Universiteit Leuven, Leuven
27. Pallares, I., Bonet, R., Garcia-Castellanos, R., Ventura, S., Aviles, FX, Vendrell, J. and Gomis-Ruth, FX (2005) Structure of human carboxypeptidase A4 with its endogenous protein inhibitor, latexin. Proc Natl Acad Sci USA, 102, pp. 3978-3983.
28. Love, RA, Parge, HE, Yu, X., Hickey, MJ, Diehl, W., Gao, J., Wriggers, H., Ekker, A., Wang, L., Thomson, JA, Dragovich, PS and Fuhrman, SA (2003) Crystallographic identification of a noncompetitive inhibitor binding site on the hepatitis C virus NS5B RNA polymerase enzyme. J Virol, 77, pp. 7575-7581.
29. Muilu, J., Torronen, A., Perakyla, M. and Rouvinen, J. (1998) Functional conformational changes of endo-1,4-xylanase II from Trichoderma reesei: A molecular dynamics study. Proteins, 31, pp. 434-444.
30. Havukainen, R., Torronen, A., Laitinen, T. and Rouvinen, J. (1996) Covalent binding of three epoxyalkyl xylosides to the active site of endo-1,4-xylanase II from Trichoderma reesei. Biochemistry, 35, pp. 9617-9624.
31. Pettersen, EF, Goddard, TD, Huang, CC, Couch, GS, Greenblatt, DM, Meng, EC and Ferrin, TE (2004) UCSF Chimera - a visualization system for exploratory research and analysis. J Comput Chem, 25, pp. 1605-1612.