DNA-binding properties of the BetI repressor protein of Escherichia coli: The inducer choline stimulates BetI-DNA complex formation

Journal of Bacteriology

Volumn 178, Issue 6, 1996, Pages 1663-1670

  Røkenes, Torunn P ^a   Lamark, Trond ^a   Strøm, Arne R ^a,b  

^a UNIVERSITY OF TROMSØ   (Norway)

^b NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

BETAINE; CHOLINE; DEOXYRIBONUCLEASE; DNA FRAGMENT; REPRESSOR PROTEIN;

ARTICLE; BINDING SITE; DNA BINDING; DNA FOOTPRINTING; DNA PROTEIN COMPLEX; ESCHERICHIA COLI; NONHUMAN; OPERON; OSMOREGULATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA INTERACTION; PROTEIN SYNTHESIS;

EID: 0029868462     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.6.1663-1670.1996     Document Type: Article

References (43)

1. Andresen, P. A., I. Kaasen, O. B. Styrvold, G. Boulnois, and A. R. Strøm. 1988. Molecular cloning, physical mapping and expression of the bet genes governing the osmoregulatory choline-glycine betaine pathway of Escherichia coli. J. Gen. Microbiol. 134:1737-1746.
2. Ansari, A. Z., J. E. Bradner, and T. V. O'Halloran. 1995. DNA-bend modulation in a repressor-to-activator switching mechanism. Nature (London) 374:371-375.
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1991. Current protocols in molecular biology. Wiley, New York.
4. Bartowsky, E., and S. Normark. 1993 Interactions of wild-type and mutant AmpR of Citrobacter freundii with target DNA. Mol. Microbiol. 10:555-565.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
6. Cren, M., A. Kondorosi, and E. Kondorosi. 1995. NoIR controls expression of the Rhizobium meliloti nodulation genes involved in the core Nod factor synthesis. Mol. Microbiol. 15:733-747.
7. Doty, S. L., M. Chang, and E. W. Nester. 1993. The chromosomal virulence gene, chvE, of Agrobacterium tumefaciens is regulated by a LysR family member J Bacteriol. 175:7880-7886.
8. Falkenberg, P., and A. R. Strøm. 1990. Purification and characterization of osmoregulatory betaine aldehyde dehydrogenase of Escherichia coli. Biochim. Biophys. Acta 1034:253-259.
9. Fried, M., and D. M. Crothers. 1981. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res. 9:6505-6525.
10. Frorath, B., C. C. Abney, H. Berthold, M. Scanarini, and W. Northemann. 1992. Production of recombinant rat interleukin-6 in Escherichia coli using a novel highly efficient expression vector pGEX-3T BioTechniques 12:558-563.
11. Guan, K. L., and J. E. Dixon, 1991. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione-S-transferase. Anal. Biochem. 192:262-267.
12. Hakes, D. J., and J. E. Dixon. 1992. New vectors for high level expression of recombinant proteins in bacteria. Anal. Biochem. 202:293-298.
13. Higgins, C. F., J. Carney, D. A. Stirling, L. Sutherland, and I. R. Booth. 1987. Osmotic regulation of gene expression: ionic strength as an intracellular signal? Trends Biochem. Sci 12:339-344.
14. Hillen, W., and C. Berens. 1994. Mechanisms underlying expression of Tn10 encoded tetracycline resistance. Annu. Rev. Microbiol 48:345-349.
15. Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28.
16. Jin, S., and A. L. Sonenshein. 1994. Transcriptional regulation of Bacillus subtilis citrate synthase genes. J. Bacteriol. 176:4680-4690
17. Kullik, I., M. B. Toledano, L. A. Tartaglia, and G. Storz. 1995. Mutational analysis of the redox-sensitive transcriptional regulator OxyR: regions important for oxidation and transcriptional activation. J. Bacteriol. 177:1275-1284.
18. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
19. Lamark, T., I. Kaasen, M. W. Eshoo, P. Falkenberg, J. McDougall, and A. R. Strøm. 1991. DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli. Mol. Microbiol. 5:1049-1064.
20. Lamark, T., T. P. Røkenes, J. McDougall, and A. R. Strøm. 1996. The complex bet promoters of Escherichia coli: regulation by oxygen (ArcA), choline (BetI), and osmotic stress. J Bacteriol 178:1655-1662
21. Lamblin, A.-F. J., and J. A. Fuchs. 1994. Functional analysis of the Escherichia coli K-12 cyn operon transcriptional regulation. J. Bacteriol. 176: 6613-6622.
22. Landfald, B., and A. R. Strøm. 1986. Choline-glycine betaine pathway confers a high level of osmotic tolerance in Escherichia coli. J. Bacteriol. 165: 849-855
23. Larsen, P. I., L. K. Sydnes, B. Landfald, and A. R. Strøm. 1987. Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose Arch Microbiol. 147:1-7.
24. Lewin, B. 1990. Genes, 4th ed. Cell Press, Cambridge, Mass.
25. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning a laboratory manual Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
26. Marines, F., and D. W. R. White. 1994 The NocR repressor-activator protein regulates expression of the nocB and nocR genes of Agrobacterium tumefaciens. Mol. Gen. Genet. 244:367-373
27. Maxam, A. M., and W. Gilbert. 1980. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 65:499-560.
28. Miller, J. H. 1972 Experiments in molecular genetics Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
29. Morissey, J. H. 1981. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal. Biochem. 117: 307-310.
30. Neidle, E. L., C. Hartnett, and L. N. Ornston. 1989. Characterization of Acinetobacter calcoaceticus catM, a repressor gene homologous in sequence to transcriptional activator genes J. Bacteriol. 171:5410-5421.
31. O'Halloran, T., and C. Walsh. 1987. Metalloregulatory DNA-binding protein encoded by the merR gene: isolation and characterization. Science 235:211-214.
32. O'Halloran, T. V., B. Frantz, M. K. Shin, D. M. Ralston, and J. G. Wright. 1989. The MerR heavy metal receptor mediates positive activation in a topologically novel transcription complex. Cell 56:119-129.
33. Parsek, M. R., M. Kivisaar, and A. M. Chakrabarty. 1995. Differential DNA bending introduced by the Pseudomonas putida LysR-type regulator, CatR, at the plasmid-borne pheBA and chromosomal catBC promoters. Mol. Microbiol. 15:819-828.
34. R promoter by salt concentration. Biochem. 24:4721-4726.
35. Schell, M. A. 1993. Molecular biology of the LysR family of transcriptional regulators. Annu. Rev. Microbiol. 47:597-626.
36. Schmitz, A., and D. J. Galas. 1979. The interaction of RNA polymerase and lac repressor with the lac control region. Nucleic Acids Res. 6:111-137.
37. Schwacha, A., and R. A. Bender. 1993. The product of the Klebsiella aerogenes nac (nitrogen assimilation control) gene is sufficient for activation of the hut operons and repression of the gdh operon. J. Bacteriol. 175:2116-2124.
38. Studier, F. W., and B. A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol. Biol. 189:113-130.
39. Tabor, S., and C. C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes Proc. Natl. Acad. Sci. USA 82:1074-1078.
40. Ueguchi, C., and T. Mizuno. 1993. The Escherichia coli nucleoid protein H-NS functions directly as a transcriptional repressor. EMBO J. 12:1039-1046.
41. Wang, L., J. D. Helmann, and S. C. Winans. 1992. The A. tumefaciens transcriptional activator OccR causes a bend at a target promoter, which is partially relaxed by a plant tumor metabolite. Cell 69:659-667.
42. Wek, R. C., and G. W. Hatfield. 1986. Nucleotide sequence and in vivo expression of the ilvY and ilvC genes in Escherichia coli K12. Transcription from divergent overlapping promoters. J. Biol. Chem 261:2441-2450
43. Wilson, R. L., and G. V. Stauffner. 1994. DNA sequence and characterization of GcvA, a LysR family regulatory protein for the Escherichia coli glycine cleavage enzyme system. J. Bacteriol. 176:2862-2868.