The Structure of a Mycobacterial Outer-Membrane Channel

Science

Volumn 303, Issue 5661, 2004, Pages 1189-1192

  Faller, Michael ^a   Niederweis, Michael ^b   Schulz, Georg E ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; BIOLOGICAL MEMBRANES; CONFORMATIONS; PHYSIOLOGICAL MODELS; PROTEINS; X RAY ANALYSIS;

CHANNEL PROTEINS;

BACTERIA;

ARGININE; BACTERIAL PROTEIN; MSPA PROTEIN, MYCOBACTERIUM SMEGMATIS; OUTER MEMBRANE PROTEIN; PORIN; PROTEIN MSPA; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

BACTERIUM;

AMINO ACID SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL OUTER MEMBRANE; BETA SHEET; CELL MEMBRANE PERMEABILITY; CHEMICAL STRUCTURE; CHEMISTRY; CRYSTALLIZATION; ELECTRIC CONDUCTIVITY; ESCHERICHIA COLI; GENETICS; HYDROGEN BOND; HYDROPHOBICITY; MEMBRANE CHANNEL; MEMBRANE PERMEABILITY; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MUTATION; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS; NONHUMAN; NUTRIENT; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DATA BANK; PROTEIN QUATERNARY STRUCTURE; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; X RAY ANALYSIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ARGININE; CELL MEMBRANE PERMEABILITY; CLONING, MOLECULAR; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ELECTRIC CONDUCTIVITY; ESCHERICHIA COLI; HYDROGEN BONDING; HYDROPHOBICITY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; MYCOBACTERIUM SMEGMATIS; PORINS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE; ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS;

EID: 1242352009     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1094114     Document Type: Article

References (34)

1. N. Rastogi, E. Legrand, C. Sola, Rev. Sci. Tech. 20, 21 (2001).
2. D. E. Minnikin, Res. Microbiol. 142, 423 (1991).
3. P. J. Brennan, H. Nikaido, Annu. Rev. Biochem. 64, 29 (1995).
4. C. E. Barry III et al., Prog. Lipid Res. 37, 143 (1998).
5. M. Daffé, P. Draper, Adv. Microb. Physiol. 39, 131 (1998).
6. P. A. Lambert, J. Appl. Microbiol. 92, 46S (2002).
7. M. Watanabe, Y. Aoyagi, M. Ridell, D. E. Minnikin, Microbiology 147, 1825 (2001).
8. B. A. Dmitriev, S. Ehlers, E. T. Rietschel, P. J. Brennan, Int. J. Med. Microbiol. 290, 251 (2000).
9. M. Jackson, D. C. Crick, P. J. Brennan, J. Biol. Chem. 275, 30092 (2000).
10. H. Nikaido, S. H. Kim, E. Y. Rosenberg, Mol. Microbiol. 8, 1025 (1993).
11. T. R. Paul, T. J. Beveridge, J. Bacteriol. 174, 6508 (1992).
12. J. Liu, E. Y. Rosenberg, H. Nikaido, Proc. Natl. Acad. Sci. U.S.A. 92, 11254 (1995).
13. C. Pitulle, M. Dorsch, J. Kazda, J. Wolters, E. Stackebrandt, Int. J. Syst. Bacteriol. 42, 337 (1992).
14. L. M. Fu, C. S. Fu-Liu, Tuberculosis 82, 85 (2002).
15. H. Engelhardt, C. Heinz, M. Niederweis, J. Biol. Chem. 277, 37567 (2002).
16. J. Trias, V. Jarlier, R. Benz, Science 258, 1479 (1992).
17. J. Trias, R. Benz, Mol. Microbiol. 14, 283 (1994).
18. M. Niederweis et al., Mol. Microbiol. 33, 933 (1999).
19. C. Stahl et al., Mol. Microbiol. 40, 451 (2001).
20. M. S. Weiss et al., FEBS Lett. 280, 379 (1990).
21. G. E. Schulz, Biochim. Biophys. Acta 1565, 308 (2002).
22. C. Heinz, S. Karosi, M. Niederweis, J. Chromatogr. B. Anal. Technol. Biomed Life Sci. 790, 337 (2003).
23. Experimental data are available as supporting material on Science Online.
24. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
25. 2, showing the dependence of the diameter on the shear number (21).
26. V. Koronakis, A. Scharff, E. Koronakis, B. Luisi, C. Hughes, Nature 405, 914 (2000).
27. L. Song et al., Science 274, 1859 (1996).
28. R. Benz, K. Bauer, Eur. J. Biochem. 176, 1 (1988).
29. J. E. W. Meyer, G. E. Schulz, Protein Sci. 6, 1084 (1997).
30. D. Bleed, C. Watt, C. Dye, "Global tuberculosis control" (WHO Report, Geneva, 2001).
31. B. Kartmann, S. Stenger, M. Niederweis, J. Bacteriol. 181, 6543, Corrigendum p. 7650 (1999).
32. T. Lichtinger et al., FEBS Lett. 454, 349 (1999).
33. E. Schiltz, A. Kreusch, U. Nestel, G. E. Schulz, Eur. J. Biochem. 199, 587 (1991).
34. The coordinates and structure factors are deposited in the Protein Data Bank under accession code 1UUN. We thank the teams of the European Molecular Biology Laboratory (EMBL)-outstation at Deutsches Elektronen-synchrotron (DESY) (Hamburg) and of the Swiss Light Source (SLS) (Villigen) for their help in data collection; and D. Frey, C. Heinz, P. Hülsmann, D. Kloer, and M. Ziegler for help at various stages of the analysis. Supported by the Deutsche Forschungsgemeinschaft under SFB-388 and Ni-412.