Expression, purification, and refolding of a novel immunotoxin containing humanized single-chain fragment variable antibody against CTLA4 and the N-terminal fragment of human perforin

Protein Expression and Purification

Volumn 48, Issue 2, 2006, Pages 307-313

  Wan, Lin ^a   Zeng, Lingyu ^a,b   Chen, Lihong ^a   Huang, Qiang ^a   Li, Shengfu ^a   Lu, Yanrong ^a   Li, Youping ^a   Cheng, Jingqiu ^a   Lu, Xiaofeng ^a  

^a SICHUAN UNIVERSITY   (China)

^b Affiliated Hospital   (China)

Author keywords

Cytotoxic T lymphocyte associated Antigen 4; Immunotoxin; In vitro refolding; Inclusion body; Single chain fragment of variable antibody

Indexed keywords

CYTOTOXIC T LYMPHOCYTE ANTIGEN 4; CYTOTOXIC T-LYMPHOCYTE ANTIGEN 4; DIFFERENTIATION ANTIGEN; HYBRID PROTEIN; IMMUNOTOXIN; LEUKOCYTE ANTIGEN; MEMBRANE PROTEIN; PEPTIDE FRAGMENT; PERFORIN; PORE FORMING CYTOTOXIC PROTEIN;

ARTICLE; BIOSYNTHESIS; CELL INCLUSION; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN FOLDING; PROTEIN RENATURATION;

ANTIGENS, CD; ANTIGENS, DIFFERENTIATION; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOTOXINS; INCLUSION BODIES; MEMBRANE GLYCOPROTEINS; PEPTIDE FRAGMENTS; PORE FORMING CYTOTOXIC PROTEINS; PROTEIN FOLDING; PROTEIN RENATURATION; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 33746218406     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.02.005     Document Type: Article

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