Comparison of enzyme immunoassay and quantitative real time PCR as evidence of Shiga toxin producing Escherichia coli (STEC) in ground meat;Vergleich von Enzym Immunoassay und Quantitativer Real Time PCR zum Nachweis von Shigatoxin Produzierenden Escherichia Coli (STEC) in Hackfleisch

Journal fur Verbraucherschutz und Lebensmittelsicherheit

Volumn 3, Issue 4, 2008, Pages 385-395

  Jahn, S ^a   Weber, H ^b   Beutin, L ^a,c  

^a FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

^b BEUTH UNIVERSITY OF APPLIED SCIENCES   (Germany)

^c Nationales Referenzlabor Escherichia Coli   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 58149398436     PISSN: 16615751     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00003-008-0377-x     Document Type: Article

References (34)

1. Bellin, T., Pulz, M., Matussek, A., Hempen, H. G. und Gunzer, F. (2001) Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes. J Clin Microbiol 39:370-374.
2. Bettelheim, K. A. und Beutin, L. (2003) Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC). J Appl Microbiol 95:205-217.
3. Beutin, L. (2006) Emerging enterohaemorrhagic Escherichia coli, causes and effects of the rise of a human pathogen. J Vet Med Series B - Infect Dis Vet Publ Health 53:299-305.
4. Beutin, L., Kruger, U., Krause, G., Miko, A., Martin, A. und Strauch, E. (2008) Evaluation of major types of Shiga toxin 2e producing Escherichia coli present in food, pigs and in the environment as potential pathogens for humans. Appl Environ Microbiol. 74:4806-4816.
5. Beutin, L., Steinruck, H., Krause, G., Steege, K., Haby, S., Hultsch, G. und Appel, B. (2007a) Comparative evaluation of the Ridascreen((R)) Verotoxin enzyme immunoassay for detection of Shiga-toxin producing strains of Escherichia coli (STEC) from food and other sources. J Appl Microbiol 102:630-639.
6. Beutin, L., Miko, A., Krause, G., Pries, K., Haby, S., Steege, K. und Albrecht, N. (2007b) Identification of human pathogenic strains of Shiga toxin-producing Escherichia coli from food by combination of serotyping and molecular typing of Shiga toxin genes. Appl Environ Microbiol 73:4769-75.
7. Bülte, M. (2003) Mikrobiologie der Lebensmittel - Fleisch und Fleischerzeugnisse. Hamburg: Behr's Verlag GmbH & Co.
8. Bülte, M. (2004) Occurrence of enterohaemorrhagic E. coli (EHEC) in domestic animals. Dtsch Tierarzt. Wochenschr 111:314-317.
9. Busch, U., Huber, I., Messelhäuser, U., Hörmansdorf er, S. und Sing, A. (2007) Nachweis Shigatoxin-bildender/Enterohämorrhagischer Escherichia coli (STEC/EHEC) mittels Real-Time PCR. J Verbr Lebensm 2:144-148.
10. Friedrich, A. W., Bielaszewska, M., Zhang, W. L., Pulz, M., Kuczius, T., Ammon, A. und Karch, H. (2002) Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. J Infect Dis 185:74-84.
11. Hartung, M. (2007) Ergebnisse der Zoonoseerhebung 2005 bei Lebensmitteln. Fleischwirtschaft 2:98-106.
12. Jahn, S. (2007) Vergleich von Enzym-Immunoassay und quantitativer Real-Time PCR zum Nachweis von Shigatoxin produzierenden Escherichia coli (STEC) in Lebensmitteln tierischer Herkunft. Diplomarbeit, Technische Fachhochschule Berlin, pp. 129.
13. Karch, H., Tarr, P. I. und Bielaszewska, M. (2005) Enterohaemorrhagic Escherichia coli in human medicine. Int J Med Microbiol 295:405-418.
14. Levine, M. M. (1987) Escherichia coli that cause diarrhea - Enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J Infect Dis 155:377-389.
15. Muniesa, M., Serra-Moreno, R. und Jofre, J. (2004) Free Shiga toxin bacteriophages isolated from sewage showed diversity although the stx genes appeared conserved. Environ Microbiol 6:716-725.
16. Paton, J. C und Patón, A. W. (2003) Methods for detection of STEC in humans. An overview. Methods Mol. Med 73:9-26.
17. Perelle, S., Dilasser, F., Grout, J. und Fach, P. (2004) Detection by 5′nuclease PCR of Shiga-toxin producing Escherichia coli O26, O55, O91, O103, O111, O113, O145 and O157:H7, associated with the world's most frequent clinical cases. Mol Cell Probes 18:185-192.
18. Persson, S., Olsen, K. E., Ethelberg, S. und Scheutz, F. (2007) Subtyping method for Escherichia coli Shiga toxin (verocytotoxin) 2 variants and correlations to clinical manifestations. J Clin Microbiol 45:2020-2024.
19. Philips, F. S., Schwartz, H. S. und Sternberg, S. S. (1960) Pharmacology of mitomycin C I. Toxicity and pathologic effects. Cancer Res 20:1354-1361.
20. Pozzi, W., Weber, H. und Beutin, L. (1996) Überleben und Nachweis von enterohämorrhagischen Escherichia coli in streichfähiger Rohwurst. Fleischwirtschaft 76:1300-1311.
21. Pulz, M., Matussek, A., Monazahian, M., Tittel, A., Nikolic, E., Hartmann, M., Beilin, T., Buer, J. und Gunzer, F. (2003) Comparison of a Shiga toxin enzyme-linked immunosorbent assay and two types of PCR for detection of Shiga toxin-producing Escherichia coli in human stool specimens. J Clin Microbiol 41:4671-4675.
22. Rangel, J. M., Sparling, P. H., Crowe, C., Griffin, P. M. und Swerdlow, D. L. (2005) Epidemiology of Escherichia coli O157 : H7 outbreaks, United States, 1982-2002. Emerg Infec Dis 11:603-609.
23. Reischl, U., Youssef, M. T., Kilwinski, J., Lehn, N., Zhang, W. L., Karch, H. und Strockbine, N. A. (2002) Real-time fluorescence PCR assays for detection and characterization of Shiga toxin, intimin, and enterohemolysin genes from Shiga toxin-producing Escherichia coll. J Clin Microbiol 402555-2565.
24. Robert Koch Institut (2006) Ausgewählte Zoonosen im Jahr 2005: Durch Lebensmittel übertragbare bakterielle gastrointestinale Infektionen. Epidemiol Bull 41:351-356.
25. Robert Koch Institut (2007) Infektionsepidemiologisches Jahrbuch meldepflichtiger Krankheiten für 2006. Berlin.
26. Robert Koch Institut (2008) Erkrankungen durch Enterohämorrhagische Escherichia coli (EHEC). Epidemiol Bull 2:11-21.
27. Rossi, C., Poli, P., Candi, A. und Buschini, A. (1997) Modulation of mitomycin C mutagenicity on Saccharomyces cerevisiae by glutathione, cytochrome P-450, and mitochondria interactions. Mutat Res 390:113-120.
28. Scheutz, F. und Strockbine, N. A. (2005) Genus I. Escherichia. In: Garrity, G. M., Brenner, D. J., Krieg, N. R. und Staley, J. T. (eds.) Bergey's Manual of Systematic Bacteriology. Springer Verlag, Heidelberg, New York, pp. 607-624.
29. Schild, T. A. (1996) Einführung in die Real-Time TaqMan PCR Technologie. Weiterstadt: Applied Biosystems GmbH.
30. Sekse, C., Solberg, A., Petersen, A., Rudi, K. und Wasteson, Y. (2005) Detection and quantification of Shiga toxin-encoding genes in sheep faeces by real-time PCR. Mol Cell Probes 19:363-370.
31. Sharma, V. K., Dean-Nystrom, E. A. und Casey, T. A. (1999) Semiautomated fluorogenic PCR assays (TaqMan) for rapid detection of Escherichia coli 0157:H7 and other Shiga toxigenic E. coli. Mol Cell Probes 13:291-302.
32. Tuttle, J., Gomez, T., Doyle, M. P., Wells, J. G., Zhao, T., Tauxe, R. V. und Griffin, P. M. (1999) Lessons from a large outbreak of Escherichia coli 0157:H7 infections: insights into the infectious dose and method of widespread contamination of hamburger patties. Epidemiol Infect 122:185-192.
33. Vimont, A., Vernozy-Rozand, C., Montet, M. P., Lazizzera, C., Bavai, C. und Delignette-Muller, M. L. (2006) Modeling and predicting the simultaneous growth of Escherichia coli O157:H7 and ground beef background microflora for various enrichment protocols. Appl Environ Microbiol 72:261-268.
34. White, D. G., Zhao, S., Sudler, R., Ayers. S., Friedman, S., Chen, S., McDermott, P. F., McDermott, S., Wagner, D. D. und Meng, J. (2001) The isolation of antibiotic-resistant Salmonella from retail ground meats. N Engl J Med 345:1147-1154.