Semi-automated fluorogenic PCR assays (TaqMan) for rapid detection of Escherichia coli O157:H7 and other Shiga toxigenic E. coli

Molecular and Cellular Probes

Volumn 13, Issue 4, 1999, Pages 291-302

  Sharma, V K ^a   Dean Nystrom, E A ^a   Casey, T A ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

EHEC; Food safety; Haemolytic uremic syndrome; Haemorrhagic colitis; Intimin; STEC

Indexed keywords

SHIGA TOXIN;

ARTICLE; AUTOMATION; BACTERIAL GENE; BACTERIAL VIRULENCE; COLONY FORMING UNIT; ESCHERICHIA COLI; FECES; FLUORESCENCE ANALYSIS; GENE AMPLIFICATION; MEAT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0032777918     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.1999.0251     Document Type: Article

References (49)

1. Karmali M. A., Petric M., Lim C., Fleming P. C., Arbus G. S., Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. Journal of Infectious Diseases. 151:1985;775-781.
2. Riley L. W., Remis R. S., Helgerson S. D. Hemorrhagic colitis associated with a rare Escherichia coli serotype. New England Journal of Medicine. 308:1983;681-685.
3. Karmali M. A. Infection by verocytotoxin-producing Escherichia coli. Clinical Microbiology Reviews. 2:1989;15-38.
4. Centers for Disease Control. Update: multistate outbreak of Escherichia coli O157:H7 infections from hamburgers in western United States, 1992-1993. Morbidity and Mortality Weekly Report. 42:1993;258-263.
5. Swinbanks D. Outbreak of EHEC infection in Japan renews concerns. Nature. 382:1996;290.
6. Bradbury J. Report on Scottish EHEC outbreak released. Lancet. 349:1997;1073.
7. Konowalchuk J., Spiers J. L., Starvic S. Vero response to a cytotoxin of Escherichia coli. Infection and Immunity. 18:1977;775-779.
8. Strockbine N. A., Marques L. R. M., Newland J. W., Smith H. W., Holmes R. K., O'Brien A. D. Two toxin-converting phages from Escherichia coli O157:H7 strain 933 encode antigenically distinct toxins with similar biologic activities. Infection and Immunity. 53:1986;135-140.
9. O'Brien A. D., Tesh V. L., Donohue-Rolfe A. Shiga toxin: biochemistry, genetics, mode of action, and role in pathogenesis. Current Topics in Microbiology and Immunology. 180:1992;65-94.
10. Donnenberg M. S., Tzipori S., McKee M. L., O'Brien A. D., Alroy J., Kaper J. B. The role of eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model. Journal of Clinical Investigation. 92:1993;1418-1424.
11. Tzipori S., Gunzer F., Donnenberg M. S. The role of eaeA gene in diarrhea and neurological complications in a gnotobiotic piglet model of enterohemorrhagic Escherichia coli infection. Infection and Immunity. 63:1995;3621-3627.
12. Obrig T. G. Pathogenesis of Shiga toxin (Verotoxin)-induced endothelial cell injury. In Hemolytic Uremic Syndrome and Thrmobocytpenic Purpura . (Kaplan, B. S., Trompeter, R. S., Moake, J. L.). 1992;405-19. New York: Marcel Dekker, Inc.
13. Moon H. W., Whipp S. C., Argenzio R. A., Levine M. M., Giannella R. A. Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infection and Immunity. 41:1983;1340-1351.
14. Wieler L. H., Schwanitz A., Vieler E. Virulence properties of Shiga toxin-producing Escherichia coli (STEC) strains of serogroup O118, a major group of STEC pathogens in calves. Journal of Clinical Microbiology. 36:1998;1604-1607.
15. Dean-Nystrom E. A., Bosworth B. T., Moon H. W., O'Brien A. D. Escherichia coli O157:H7 requires intimin for enteropathogenicity in calves. Infection and Immunity. 66:1998;4560-4563.
16. Hancock D. D., Besser T. E., Kinsel M. K., Tarr P. I., Rice D. H., Paros M. G. The prevalence of Escherichia coli O157:H7 in dairy and beef cattle in Washington State. Epidemiology and Infection. 113:1994;199-207.
17. Smith H. R., Scotland S. M. Isolation and identification methods for Escherichia coli O157:H7 and other Vero cytotoxin producing strains. Journal of Clinical Pathology. 46:1993;10-17.
18. Acheson D. W. K., DeBreucker S., Donohue-Rolfe A., Kozak K., Yi A., Keusch G. T. Development of a clinically useful diagnostic enzyme immunoassays for enterohemorrhagic Escherichia coli infection. In Recent Advances in Verocytotoxin Producing Escherichia coli Infections. 1994;109-12. Amsterdam, The Netherlands: Elsevier Science B. V.
19. Gannon V. P. J., Teerling C., Masri S., Gyles C. L. Molecular cloning and nucleotide sequence of another variant of the Escherichia coli Shiga-like toxin family. Journal of General Microbiology. 136:1990;1125-1135.
20. Ito H., Nishibuchi M., Takeda Y. Analysis of the antigenic differences between Verotoxin 2 (VT2) and VT2 (VT2vh) of Verotoxin-producing Escherichia coli by site directed mutagenesis. FEMS Microbiology Letters. 79:1991;27-30.
21. March S. B., Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. Journal of Clinical Microbiology. 23:1986;869-872.
22. Ojeda A., Prado V., Martinez J. Sorbitol-negative phenotype among enterohemorrhagic Escherichia coli strains of different serotypes and from different sources. Journal of Clinical Microbiology. 33:1995;2199-2201.
23. Sowers E. G., Wells J. G., Strockbine N. A. Evaluation of commercial latex reagents for identification of O157 and H7 antigens of Escherichia coli. Journal of Clinical Microbiology. 34:1996;1286-1289.
24. Samadpour M., Liston J., Ongerth J. E., Tarr P. I. Evaluation of DNA probes for detection of Shiga-like toxin producing Escherichia coli in foods and calf fecal samples. Applied and Environmental Microbiology. 56:1990;1212-1215.
25. Karch H., Meyer T. Single primer pair for amplifying segments of distinct Shiga-like toxin genes by polymerase chain reaction. Journal of Clinical Microbiology. 27:1989;2751-2757.
26. Witham P. K., Yamashiro C. T., Livak K. J., Batt C. A. A PCR-based assay for the detection of Escherichia coli Shiga-like toxin genes in ground beef. Applied and Environmental Microbiology. 62:1996;1347-1353.
27. Ramotar K., Waldhart B., Church D., Szumski R., Louie T. J. Direct detection of verotoxin-producing Escherichia coli in stool samples by PCR. Journal of Clinical Microbiology. 33:1995;519-524.
28. Gannon V. P. J., Rashed M., King R. K., Thomas E. J. G. Detection and characterization of the eae gene of Shiga-like toxin-producing Escherichia coli by using polymerase chain reaction. Journal of Clinical Microbiology. 31:1993;1268-1274.
29. Louie M., De Azavedo J., Clarke R. Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers. Epidemiology and Infection. 112:1994;449-461.
30. Matsuura M., Yamashiro C., Flood S., Paszko-Kolva C. Detection of Salmonella in food using a fluorogenic 5′ nuclease assay. American Environmental Laboratory. 1997;24-25.
31. Bassler H. A., Flood S. J. A., Livak K. J., Marmaro J., Knorr R., Batt C. A. Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes. Applied and Environmental Microbiology. 61:1995;3724-3728.
32. Marques L. R. M., Cryz J. S. M., O'Brien A. D. Escherichia coli strains isolated from pigs with edema disease produce a variant of Shiga-like toxin II. FEMS Microbiology Letters. 44:1987;33-38.
33. Doyle M. P., Schoeni J. L. Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry. Applied and Environmental Microbiology. 53:1987;2394-2396.
34. Fratamico P. M., Sackitey S. K., Weidmann M., Deng M. Y. Detection of Escherichia coli O157:H7 by multiplex PCR. Journal of Clinical Microbiology. 33:1995;2188-2191.
35. Calderwood S. B., Auclair F., Donohue-Rolfe A., Keusch G. T., Mekalanos J. J. Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli. Proceedings of National Academy of Sciences USA. 84:1987;4364-4368.
36. Jackson M. P., Neill R. J., O'Brien A. D., Holmes R. K., Newland J. W. Nucleotide sequence analysis and comparison of the structural genes for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages from Escherichia coli. FEMS Microbiology Letters. 44:1987;109-114.
37. Beebakhee G., Louie M., De Azavedo J., Brunton J. Cloning and nucleotide sequence of the eae gene homologue from enterohemorrhagic Escherichia coli serotype O157:H7. FEMS Microbiology Letters. 91:1992;63-68.
38. McGraw E. A., Li J., Selander R. K., Whittam T. S. Molecular evolution and mosaic structure of alpha, beta, and gamma intimins of pathogenic Escherichia coli. Molecular Biology and Evolution. 16:1999;12-22.
39. Oberst R. D., Hays M. P., Bohra L. K. PCR-based amplification and presumptive detection of Escherichia coli O157:H7 with an internal fluorogenic probe and the 5′ nuclease (TaqMan) assay. Applied and Environmental Microbiology. 64:1998;3389-3396.
40. Meng J., Zhao S., Doyle M. P., Mitchell S. E., Kresovich S. A multiplex PCR for identifying Shiga-like toxin-producing Escherichia coli O157:H7. Letters in Applied Microbiology. 24:1997;172-176.
41. Willshaw G. A., Thirlwell J., Jones A. P., Parry S., Salmon R. L., Hickey M. Vero cytotoxin-producing Escherichia coli O157:H7 in beef burgers linked to an outbreak of diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome in Britain. Letters in Applied Microbiology. 19:1994;304-307.
42. Read S. C., Clarke R. C., Martin A., De Grandis S. A., Hii J., McEwen S., Gyles C. L. Polymerase chain reaction for detection of verotoxigenic Escherichia coli isolated from animal and food sources. Molecular and Cellular Probes. 6:1992;153-161.
43. Cebula T. A., Payne W. L., Feng P. Simultaneous identification of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR. Journal of Clinical Microbiology. 33:1995;248-250.
44. 0157. Journal of Clinical Microbiology. 36:1998;598-602.
45. Gannon V. P. J., D'Souza S., Graham T., King R. K., Rahn K., Read S. Use of flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli. Journal of Clinical Microbiology. 35:1997;656-662.
46. Nataro J. P., Kaper J. B. Diarrheagenic Escherichia coli. Clinical Microbiology Reviews. 11:1998;142-201.
47. Bilge S. S., Vary J. C., Dowell S. F., Tarr P. I. Role of the Escherichia coli O157:H7 O side chain in adherence and analysis of an rfb locus. Infection and Immunity. 64:1996;4795-4801.
48. Chen S., Xu R., Yee A. An automated fluorogenic PCR method for detection of Shiga toxin-producing Escherichia coli in foods. Applied Environmental Microbiology. 64:1998;4210-4216.
49. Department of Agriculture, Food Safety and Inspection Service. Pathogen Reduction: Hazard Analysis and Critical Control Points (HACCP) Systems; Final Rule. Federal Register. 61:1996;38,805-55.