Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC)

Journal of Applied Microbiology

Volumn 95, Issue 2, 2003, Pages 205-217

  Bettelheim, K A ^a   Beutin, L ^b  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b ROBERT KOCH INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DIAGNOSIS; DISEASE CONTROL; PATIENT TREATMENT;

'CURRENT; CLINICAL SAMPLES; ENVIRONMENTAL SAMPLE; FALSE NEGATIVES; FALSE POSITIVE; FOOD SAMPLES; MOLECULAR-BIOLOGICAL TECHNIQUES; SEROTYPES; SHIGA TOXIN-PRODUCING ESCHERICHIA COLI; TEST SYSTEMS;

ESCHERICHIA COLI;

BACTERIAL DNA; SHIGA TOXIN; VEROTOXIN;

IDENTIFICATION METHOD; MICROBIOLOGY; PATHOGEN; TOXIN;

BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CLINICAL LABORATORY; DNA HYBRIDIZATION; DNA SEQUENCE; ENTEROBACTER INFECTION; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMAN; IMMUNOBLOTTING; LATEX AGGLUTINATION TEST; MOLECULAR BIOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; REVIEW; SEROLOGY; TISSUE CULTURE; VERO CELL;

BACTERIA (MICROORGANISMS); ENTEROBACTER; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0042661253     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2003.02031.x     Document Type: Review

References (103)

1. Arthur, T.M., Barkocy-Gallagher, G.A., Rivera-Betancourt, M. and Koohmaraie, M. (2002) Prevalence and characterization of non-0157 Shiga toxin-producing Escherichia coli on carcasses in commercial beef cattle processing plants. Applied and Environmental Microbiology 68, 4847-4852.
2. Bastian, S.N., Carle, I. and Grimont, F. (1998) Comparison of 14 PCR systems for the detection and subtyping of stx genes in Shiga-toxin-producing Escherichia coli. Research in Microbiology 149, 457-472.
3. Bélanger, S.D., Boissinot, M., Ménard, C., Picard, F.J. and Bergeron, M.G. (2002) Rapid detection of Shiga toxin-producing bacteria in feces by multiplex PCR with molecular beacons on the smart cycler. Journal of Clinical Microbiology 40, 1436-1440.
4. Bellin, T., Pulz, M., Matussek, A., Hempen, H.G. and Gunzer, F. (2001) Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes. Journal of Clinical Microbiology 39, 370-374.
5. Belnap, W.D. and O'Donnell, J.J. (1955) Epidemic gastroenteritis due to Escherichia coli O-111. Journal of Pediatrics 47, 178-193.
6. Bettelheim, K.A. (1995) Identification of enterohaemorrhagic Escherichia coli by means of their production of enterohaemolysin. Journal of Applied Bacteriology 79, 178-180.
7. Bettelheim, K.A. (1997) Biochemical studies on enterohaemorrhagic Escherichia coli (EHEC). In Coliforms and E. coli Problem or Solution ed. Kay, D. and Fricker, C. pp. 243-248. Cambridge, UK: The Royal Society of Chemistry Information Services.
8. Bettelheim, K.A. (1998a) Studies of Escherichia coli cultured on Rainbow™ agar O157 with particular reference to enterohaemorrhagic Escherichia coli (EHEC). Microbiology and Immunology 42, 265-269.
9. Bettelheim, K.A. (1998b) Reliability CHROMagar® for the detection of enterohaemorrhagic Escherichia coli (EHEC) O157 but not EHEC belonging to other serogroups. Journal of Applied Microbiology 85, 425-428.
10. Bettelheim, K.A. (2000) Role of non-O157 VTEC. Journal of Applied Microbiology 88(Symposium Suppl.), 38S-50S.
11. Bettelheim, K.A. (2001) Development of a rapid method for the detection of verocytotoxin-producing Escherichia coli (VTEC). Letters in Applied Microbiology 33, 31-35.
12. Bettelheim, K.A., Maskill, W.J. and Pearce, J. (1983) Comparison of the standard tube and microagglutination techniques for determining Brucella antibodies. Journal of Hygiene Cambridge 90, 33-39.
13. Bettelheim, K.A., Gracey, M. and Wadström, T. (1985) The use of the coagglutination test to determine whether Australian and New Zealand isolates of Escherichia coli produce the heat-labile enterotoxin. Zentralblatt für Bakteriologie Mikrobiologie und Hygiene, I. Abteilung Originale A 260, 293-296.
14. Bettelheim, K.A., Whipp, M., Djordjevic, S.P. and Ramachandran, V. (2002) First isolation outside Europe of sorbitol-fermenting verocytotoxigenic Escherichia coli (VTEC) belonging to O group O157. Journal of Medical Microbiology 51, 713-714.
15. Beutin, L., Montenegro, M.A., Ørskov, I., Ørskov, F., Prada, J., Zimmermann, S. and Stephan, R. (1989) Close association of verocytotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli. Journal of Clinical Microbiology 27, 2559-2564.
16. Beutin, L., Geier, D., Steinrück, H., Zimmermann, S. and Scheutz, F. (1993) Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. Journal of Clinical Microbiology 31, 2483-2488.
17. Beutin, L., Zimmermann, S. and Gleier, K. (1996a) Pseudomonas aeruginosa can cause false-positive identification of verotoxin (Shiga-like toxin) production by a commercial enzyme immun assay system for the detection of Shiga-like toxins (SLTs). Infection 24, 267-268.
18. Beutin, L., Zimmermann, S. and Gleier, K. (1996b) Rapid detection and isolation of Shiga-like toxin (verocytotoxin)-producing Escherichia coli by direct testing of individual colonies from washed sheep blood agar plates in the VTEC-RPLA assay. Journal of Clinical Microbiology 34, 2812-2814.
19. Beutin, L., Geier, D., Zimmermann, S., Aleksic, S., Gillespie, H.A. and Whittam, T.S. (1997a) Epidemiological relatedness and clonal types of natural populations of Escherichia coli strains producing Shiga toxins in separate populations of cattle and sheep. Applied and Environmental Microbiology 63, 2175-2180.
20. Beutin, L., Horbach, I., Zimmermann, S. and Gleier, K. (1997b) Vergleich verschiedener diagnostischer Methoden zum Nachweis von Verotoxin (Shiga-toxin) bildenden Escherichia coli Stämmen (VTEC) aus klinischen Stuhlproben. Journal of Laboratory Medicine 21, 537-546.
21. Beutin, L., Gleier, K. and Zimmermann, S. (1998) Comparative evaluation of different diagnostic methods for detection of Shiga-toxin producing strains of Escherichia coli (STEC) in human clinical stool specimens. In: Verocytotoxigenic E. coli in Europe. Concerted Action CT98-3935. 1. Methods for Verocytotoxigenic E. coli ed. Duffy, G., Garvey, P., Coia, J., Wasteson, Y. and McDowell, D.A. pp. 15-24. Dublin, Ireland: Teagasc, The National Food Centre. http://www.research.teagasc.ie/vteceurope/publications.html.
22. Beutin, L., Gleier, K. and Zimmermann, S. (1999) The new VT2 variant, VT2d-Ount, is not detected by the VTEC-RPLA assay. Notiziario dell'Istituto Superiore di Sanità IVC News 12, (Suppl. 1), 3.
23. Beutin, L., Kaulfuss, S., Cheasty, T., Brandenburg, B.B., Zimmermann, S., Gleier, K., Willshaw, G.A. and Smith, H.R. (2002a) Characteristics and association with disease of two major subclones of Shiga toxin (verocytotoxin)-producing strains of Escherichia coli (STEC) O157 that are present among isolates from patients in Germany. Diagnostic Microbiology and Infectious Disease 44, 337-346.
24. Beutin, L., Zimmermann, S. and Gleier, K. (2002b) Evaluation of the VTEC-screen 'Seiken' test for detection of different types of Shiga toxin (verotoxin)-producing Escherichia coli (STEC) in human stool samples. Diagnostic Microbiology and Infectious Disease 42, 1-8.
25. Bitzan, M. and Karch, H. (2003) Serological methods for the detection of STEC infections. In E. coli Shiga Toxin Methods and Protocols ed. Philpott, D. and Ebel, F. pp. 27-43. NJ, USA: Humana Press.
26. Blanco, M., Blanco, J.E., Blanco, J., Mora, A., Prado, C., Alonso, M.P., Mourino, M., Madrid, C., et al. (1997) Distribution and characterization of faecal verotoxin-producing Escherichia coli (VTEC) isolated from healthy cattle. Veterinary Microbiology 54, 309-319.
27. Boerlin, P., McEwen, S.A., Boerlin-Petzold, F., Wilson, J.B., Johnson, R.P and Gyles, C.L. (1999) Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans. Journal of Clinical Microbiology 37, 497-503.
28. Bonardi, S., Foni, E. and Maggi, E. (2000) Comparison of Vero cell assay, polymerase chain reaction and an enzyme immunoassay for identification of verocytotoxin-producing Escherichia coli O157:H7. New Microbiology 23, 47-53.
29. Bouvet, J., Bavai, C., Rossel, R., Le Roux, A., Montet, M.P., Ray-Gueniot, S., Mazuy, C., Attache, V., et al. (2002a) Effects of cutting process on pork meat contamination by verotoxin-producing Escherichia coli (VTEC) and E. coli O157:H7. International Journal of Food Microbiology 77, 91-97.
30. Bouvet, J., Montet, M.P., Rossel, R., Le Roux, A., Bavai, C., Ray-Gueniot, S., Mazuy, C., Attache, V., et al. (2002b) Effects of slaughter processes on pig carcass contamination by verotoxin-producing Escherichia coli and E. coli O157:H7. International Journal of Food Microbiology 77, 99-108.
31. Brett, K.N., Ramachandran, V., Hornitzky, M.A., Bettelheim, K.A., Walker, M.J. and Djordjevic, S.P. (2003) stx1c Is the most common Shiga toxin 1 subtype among Shiga toxin-producing Escherichia coli isolates from sheep but not among isolates from cattle. Journal of Clinical Microbiology 41, 926-936.
32. Calderwood, S., Acheson, D.W.K., Keusch, G.T., Barrett, T.J., Griffin, P.M., Strockbine, N.A., Swaminathan, B., Kaper, J.B., et al. (1996) Proposed new nomenclature for SLT (VT) family. ASM News 62, 118-119.
33. Cermelli, C., Fabio, G., Casolari, C. and Quaglio, P. (2002) Evaluation of a VERO cell toxicity test to detect EHEC infection. Microbiologica 25, 235-238.
34. Chart, H., Willshaw, G.A. and Cheasty, T. (2001) Evaluation of a reversed passive latex agglutination test for the detection of verocytotoxin (VT) expressed by strains of VT-producing Escherichia coli. Letters in Applied Microbiology 32, 370-374.
35. Conradi, H. (1903) Über lösliche durch durch aseptische Autolyse, erhaltene Giftstoffe von Ruhr- und Typhus Bazillen. Deutsche Medizinische Wochenschrift 29, 26-28.
36. Djordjevic, S.P., Hornitzky, M.A., Bailey, G., Gill, P., Vanselow, B., Walker, K. and Bettelheim, K.A. (2001) Virulence properties and serotypes of Shiga toxin-producing Escherichia coli from healthy Australian slaughter-age sheep. Journal of Clinical Microbiology 39, 2017-2021.
37. Donnenberg, M.S. and Whittam, T.S. (2001) Pathogenesis and evolution of virulence in enteropathogenic and enterohemorrhagic Escherichia coli. Journal of Clinical Investigation 107, 539-548.
38. Fagan, P.K., Hornitzky, M.A., Bettelheim, K.A. and Djordjevic, S.P. (1999) Detection of Shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorhagic Escherichia coli (EHEC) Hemolysin EHEC (hlyA) genes in animal feces by multiplex PCR. Applied and Environmental Microbiology 65, 868-872.
39. Feldsine, P.T., Kerr, D.E., Leung, S.C., Lienau, A.H., Moser, R.F. and Mui, L.A. (2002a) Visual immunoprecipitate assay eight hour method for detection of enterohemorrhagic Escherichia coli O157:H7 in raw and cooked beef (modification of AOAC official method 996.09): collaborative study. Journal of AOAC International 85, 1029-1036.
40. Feldsine, P.T., Kerr, D.E., Leung, S.C., Lienau, A.H., Miller, S.M. and Mui, L.A. (2002b) Assurance (R) enzyme immunoassay eight hour method for detection of enterohemorrhagic Escherichia coli O157:H7 in raw and cooked beef (modification of AOAC official method 996.10): collaborative study. Journal of AOAC International 85, 1037-1044.
41. Feng, P., Lampel, K.A., Karch, H. and Whittam, T.S. (1998) Genotypic and phenotypic changes in the emergence of Escherichia coli O157:H7. Journal of Infectious Diseases 177, 1750-1753.
42. Frias, C., Majo, M., Margall, N., Llobet, T., Mirelis, B. and Peats, G. (1996) Evaluation of an enzyme immunoassay for verotoxin detection in Escherichia coli. Microbiologica 12, 395-404.
43. Friedrich, A.W., Bielaszewska, M. and Karch, H. (2002a) Diagnosis of Shiga toxin-producing Escherichia coli infections. Journal of Laboratory Medicine 26, 183-190.
44. Friedrich, A.W., Bielaszewska, M., Zhang, W.L., Pulz, M., Kuczius, T., Ammon, A. and Karch, H. (2002b) Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. Journal of Infectious Diseases 185, 74-84.
45. Gallien, P., Klie, H., Perlberg, K.W. and Protz, D. (1996) Utilization of nylon membranes for the specified isolation and characterization of verotoxin-producing Escherichia coli by using DNA-probes. Berliner und Münchener Tierärztliche Wochenschrift 109, 431-433.
46. Gallien, V.P., Richter, H., Klie, H., Timm, M., Karch, H., Lehman, S., Perlberg, K.-W., Teufel, P., et al. (1998) Detection of Shigatoxin producing Escherichia coli (STEC) in foods and characterization of isolates. Bundesgesundheitsblatt 41 (Sonderheft Oktober), 26-30.
47. Ge, Bi., Zhao, S.H., Hall, R. and Meng, J.H. (2002) A PCR-ELISA for detecting Shiga toxin-producing Escherichia coli. Microbes and Infection 4, 285-290.
48. Geue, L., Segura-Alvarez, M., Conraths, F.J., Kuczius, T., Bockemühl, J., Karch, H. and Gallien, P. (2002) A long-term study on the prevalence of Shiga toxin-producing Escherichia coli (STEC) on four German cattle farms. Epidemiology and Infection 129, 173-185.
49. Gioffré, A., Meichtri, L., Miliwebsky, E., Baschkier, A., Chillemi, G., Romano, M.I., Estani, S.S., Cataldi, A., et al. (2002) Detection of Shiga toxin-producing Escherichia coli by PCR in cattle in Argentina. Evaluation of two procedures. Veterinary Microbiology 87, 301-313.
50. Goldwater, P.N. and Bettelheim, K.A. (1996) An outbreak of hemolytic uremic syndrome due to Escherichia coli O157:H7: or was it? Emerging Infectious Diseases 2, 153-154.
51. Hiramatsu, R., Matsumoto, M., Miwa, Y., Suzuki, Y., Saito, M. and Miyazaki, Y. (2002) Characterization of Shiga toxin-producing Escherichia coli O26 strains and establishment of selective isolation media for these strains. Journal of Clinical Microbiology 40, 922-925.
52. Hornitzky, M.A., Bettelheim, K.A. and Djordjevic, S.P. (2001) The detection of Shiga toxin-producing Escherichia coli in diagnostic bovine faecal samples using vancomycin-cefixime-cefsulodin blood agar and PCR. FEMS Microbiology Letters 198, 17-22.
53. Jackson, M.P., Newland, J.W., Holmes, R.K. and O'Brien, A.D. (1987) Nucleotide sequence analysis of the structural genes for Shiga-like toxin I encoded by bacteriophage 933J from Escherichia coli. Microbial Pathogenesis 2, 147-153.
54. Jiang, X., Wilton, N., Jing, E., Zhong, W., Warren, D., Rose, L., Nycum, L., Jordan, D., et al. (2001) University outbreak of calicivirus infection mistakenly attributed to Shiga toxin-producing Escherichia coli O157:H7 - Virginia, 2000 (Reprinted from MMWR, (2001) Vol. 50, pp. 489-491). Journal of the American Medical Association 286, 162.
55. Karch, H. and Bielaszewska, M. (2001) Sorbitol-fermenting Shiga toxin-producing Escherichia coli O157:H-strains: epidemiology, phenotypic and molecular characteristics, and microbiological diagnosis. Journal of Clinical Microbiology 39, 2043-2049.
56. Karch, H., Meyer, T., Russmann, H. and Heesemann, J. (1992) Frequent loss of Shiga-like toxin genes in clinical isolates of Escherichia coli upon subcultivation. Infection & Immunity 60, 3464-3467.
57. Karmali, M.A. (1989) Infection by verocytotoxin-producing Escherichia coli. Clinical Microbiological Reviews 2, 15-38.
58. Karmali, M.A., Steel, B.T., Petric, M. and Lim, C. (1983) Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools. Lancet 1, 619-620.
59. Karmali, M.A., Petric, M. and Bielaszewska, M. (1999) Evaluation of a microplate latex agglutination method (verotox-F-assay) for detecting and characterizing verotoxins (Shiga toxins) in Escherichia coli. Journal of Clinical Microbiology 37, 396-399.
60. Kehl, K.S., Havens, P., Behnke, C.E. and Acheson, D.W. (1997) Evaluation of the premier EHEC assay for detection of Shiga toxin-producing Escherichia coli. Journal of Clinical Microbiology 35, 2051-2054.
61. Khan, A., Yamasaki, S., Sato, T., Ramamurthy, T., Pal, A., Datta, S., Chowdhury, N.R., Das, S.C., et al. (2002) Prevalence and genetic profiling of virulence determinants of non-O157 Shiga toxin-producing Escherichia coli isolated from cattle, beef, and humans, Calcutta, India. Emerging Infectious Diseases 8, 54-62.
62. Kirchgatterer, A., Weber, T., Hinterreiter, M., Knoflach, P. and Allerberger, F. (2002) Haemorrhagic colitis due to Escherichia coli O103:H2 associated with infliximab therapy in a patient with rheumatoid arthritis. Rheumatology 41, 355-356.
63. Klein, E.J., Stapp, J.R., Clausen, C.R., Boster, D.R., Wells, J.G., Qin, X., Swerdlow, D.L. and Tarr, P.I. (2002) Shiga toxin-producing Escherichia coli in children with diarrhea: a prospective point-of-care study. Journal of Pediatrics 141, 172-177.
64. Koch, C., Hertwig, S., Lurz, R., Appel, B. and Beutin, L. (2001) Isolation of a lysogenic bacteriophage carrying the stx(1(OX3)) gene, which is closely associated with Shiga toxin-producing Escherichia coli strains from sheep and humans. Journal of Clinical Microbiology 39, 3992-3998.
65. Konowalchuk, J., Speirs, J.L. and Stavric, S. (1977) Vero response to a cytotoxin of Escherichia coli. Infection and Immunity 18, 775-779.
66. Kudva, I.T., Hatfield, P.G. and Hovde, C.J. (1997) Characterization of Escherichia coli O157:H7 and other Shiga toxin-producing E. coli serotypes isolated from sheep. Journal of Clinical Microbiology 35, 892-899.
67. Kulkarni, H., Goldwater, P.N., Martin, A. and Bettelheim, K.A. (2002) Escherichia coli 'O' group serological responses and clinical correlations in epidemic HUS patients. Comparative Immunology Microbiology and Infectious Diseases 2, 249-268.
68. Lehmacher, A., Meier, H., Aleksic, S. and Bockemühl, J. (1998) Detection of hemolysin variants of Shiga toxin-producing Escherichia coli by PCR and culture on vancomycin-cefixime-cefsulodin blood agar. Applied and Environmental Microbiology 64, 2449-2453.
69. Ludwig, K., Sarkim, V., Bitzan, M., Karmali, M.A., Bobrowski, C., Ruder, H, Laufs, R., Sobottka, I., et al. (2002) Shiga toxin-producing Escherichia coli infection and antibodies against Stx2 and Stx1 in household contacts of children with enteropathic hemolytic-uremic syndrome. Journal of Clinical Microbiology 40, 1773-1782.
70. McIngvale, S.C., Elhanafi, D. and Drake, M.A. (2002) Optimization of reverse transcriptase PCR to detect viable Shiga-toxin-producing Escherichia coli. Applied and Environmental Microbiology 68, 799-806.
71. Mackenzie A.M., Lebel, P., Orrbine, E., Rowe, P.C., Hyde, L., Chan, F., Johnson, W. and McLaine, P.N. (1998) Sensitivities and specificities of premier E. coli O157 and premier EHEC enzyme immunoassays for diagnosis of infection with verotoxin (Shiga-like toxin)-producing Escherichia coli. The SYNSORB Pk Study investigators. Journal of Clinical Microbiology 36, 1608-1611.
72. March, S.B. and Ratnam, S. (1986) Sorbitol-MacConkey medium for detection of Escherichia coli O157.H7 associated with haemorrhagic colitis. Journal of Clinical Microbiology 23, 869-872.
73. Marshall, J.A., Yuen, L.K.W., Catton, M.G., Guneskere, I.C., Wright, P.J., Bettelheim, K.A., Griffith, J.M., Lightfoot, D., et al. (2001) Multiple outbreaks of Norwalk-like virus gastro-enteritis associated with a Mediterranean-style restaurant. Journal of Medical Microbiology 50, 143-151.
74. Melton-Celsa, A. and O'Brien, A. (2003) Plant and bacterial toxins as RNA N-glycosidases. In Bacterial Protein Toxins ed. Burns, D., Barbieri, J.T., Iglewski, B.H. and Rappuoli, R. pp. 245-255. Washington, DC: ASM Press.
75. Neisser, M. and Shiga, K. (1903) Ueber freie receptoren von typhus- und dysenteriebazillen und über das dysenterietoxin. Deutsche Medizinische Wochenschrift 29, 61-62.
76. Nishikawa, Y., Zhou, Z., Hase, A., Ogasawara, J., Cheasty, T. and Haruki, K. (2000) Relationship of genetic type of Shiga toxin to manifestation of bloody diarrhea due to enterohemorrhagic Escherichia coli serogroup O157 isolates in Osaka City, Japan. Journal of Clinical Microbiology 38, 2440-2442.
77. Osek, J. (2002) Rapid and specific identification of Shiga toxin-producing Escherichia coli in faeces by multiplex PCR. Letters in Applied Microbiology 34, 304-310.
78. Parreira, V.R. and Gyles, C.L. (2002) Shiga toxin genes in avian Escherichia coli. Veterinary Microbiology 87, 341-352.
79. Paton, J.C. and Paton, A.W. (1998) Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clinical Microbiological Reviews 11, 450-479.
80. Pièrard, D., Muyldermans, G., Moriau, L., Stevens, D. and Lauwers, S. (1998) Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolates. Journal of Clinical Microbiology 36, 3317-3322.
81. Pradel, N., Livrelli, V., De Champs, C., Palcoux, J.B., Reynaud, A., Scheutz, F., Sirot, J., Joly, B., et al. (2000) Prevalence and characterization of Shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one-year prospective study in France. Journal of Clinical Microbiology 38, 1023-1031.
82. Ramachandran, V., Hornitzky, M.A., Bettelheim, KA., Walker, M.J. and Djordjevic, S.P. (2001) The common ovine Shiga toxin 2-containing Escherichia coli serotypes and human isolates of the same serotypes possess a Stx2d toxin type. Journal of Clinical Microbiology 39, 1932-1937.
83. Reischl, U., Youssef, M.T., Kilwinski, J., Lehn, N., Zhang, W.L., Karch, H. and Strockbine, N.A. (2002) Real-time fluorescence PCR assays for detection and characterization of Shiga toxin, intimin, and enterohemolysin genes from Shiga toxin-producing Escherichia coli. Journal of Clinical Microbiology 40, 2555-2565.
84. Riley, L.W., Remis, R.S., Helgerson, S.D., McGee, H.B., Wells, G.J., Davis, B.R., Herbert, R.J., Olcott, E.S., et al. (1983) Hemorrhagic colitis associated with a rare Escherichia coli serotype. New England Journal of Medicine 308, 681-685.
85. Rüger, H.J., Karch, H., Timm, M., Richter, H., Musielski, H., Rüger, K., Pollex, G. and Mohr, J. (2001) Development and evaluation of a colony immunoblot assay for isolation of Shiga-toxin producing Escherichia coli. DGHM Congress, Aachen, 2001. International Journal of Medical Microbiology 291, PS-P8.
86. Scheutz, F., Beutin, L. and Smith, H. (2001a) Clinical detection of verocytotoxin-producing E. coli (VTEC). In Verocytotoxigenic E. coli ed. Duffer, G., Garvey, P. and McDowell, D. pp. 25-56. Trumbull, CT, USA: Food and Nutrition Press Inc.
87. Scheutz, F., Beutin, L., Pièrard, D. and Smith, H. (2001b) Nomenclature of verocytotoxins. In Verocytotoxigenic E. coli ed. Duffy, G., Garvey, P. and McDowell, D. pp. 447-452. Trumbull, CT, USA: Food and Nutrition Press Inc.
88. Schmidt, H., Beutin, L. and Karch, H. (1995) Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL933. Infection and Immunity 63, 1055-1061.
89. Schmidt, H., Geitz, C., Tarr, P.I., Frosch, M. and Karch, H. (1999) Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonality. Journal of Infectious Diseases 179, 115-123.
90. Schmidt, H., Scheef, J., Morabito, S., Caprioli, A., Wider, L.H. and Karch, H. (2000) A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons. Applied & Environmental Microbiology 66, 1205-1208.
91. Sharma, V.K. (2002) Detection and quantitation of enterohemorrhagic Escherichia coli O157, O111, and O26 in beef and bovine feces by real-time polymerase chain reaction. Journal of Food Protection 65, 1371-1380.
92. Sidjabat-Tambunan, H., Bensink, J. and Bettelheim, K.A. (1998) Isolation of verocytotoxin-producing Escherichia coli from mutton carcases. Australian Veterinary Journal 76, 364-365.
93. Suthienkul, O., Brown, J.E., Seriwatana, J., Tienthongdee, S., Sastravaha, S. and Echeverria, P. (1990) Shiga-like-toxin producing Escherichia coli in retail meats and cattle in Thailand. Applied and Environmental Microbiology 56, 1135-1139.
94. Taneike, I., Zhang, H.M., Wakisaka-Saito, N. and Yamamoto, T. (2002) Enterohemolysin operon of Shiga toxin-producing Escherichia coli: a virulence function of inflammatory cytokine production from human monocytes. FEBS Letters 524, 219-224.
95. Timm, M., Klie, H., Richter, H. and Perlberg, K.W. (1996) A method for specific isolation of verotoxin-producing Escherichia coli-colonies. Berliner und Münchener Tierärztliche Wochenschrift 109, 270-272.
96. Timm, M., Klie, H., Richter, H., Gallien, V.P., Perlberg, K.-W., Lehman, S. and Protz, D. (1998) Verfahren zum qualitativen Nachweis von verotoxin-produzierenden Escherichia coli (VTEC) in Lebensmitteln und Fäces. Bundesgesundheitsblatt, Sonderdruck Oktober 41 (Sonderheft Oktober), 20-25.
97. Tomoyasu, T. (1998) Improvement of the immunomagnetic separation method selective for Escherichia coli O157 strains. Applied and Environmental Microbiology 64, 376-382.
98. Urdahl, A.M., Cudjoe, K., Wahl, E., Heir, E. and Wasteson, Y. (2002) Isolation of Shiga toxin-producing Escherichia coli O103 from sheep using automated immunomagnetic separation (AIMS) and AIMS-ELISA: sheep as the source of a clinical E. coli O103 case? Letters in Applied Microbiology, 35, 218-222.
99. Wade, W.G., Thom, B.T. and Evans, B.T. (1979) Cytotoxic enteropathogenic Escherichia coli. The Lancet ii, 1235-1236.
100. Werber, D., Fruth, A., Liesegang, A., Littmann, M., Buchholz, U., Prager, R., Karch, H., Breuer, T., et al. (2002) A multistate outbreak of Shiga toxin-producing Escherichia coli O26:H11 infections in Germany, detected by molecular subtyping surveillance. Journal of Infectious Diseases 186, 419-422.
101. Whittam, T.S., Wachsmuth, I.K. and Wilson, R.A. (1988) Genetic evidence of clonal descent of Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome. Journal of Infectious Diseases 157, 1124-1133.
102. Wilson, M.W. and Bettelheim, K.A. (1980) Cytotoxic Escherichia coli serotypes. The Lancet i, 201.
103. Ziebell, K.A., Read, S.C., Johnson, R.P. and Gyles, C.L. (2002) Evaluation of PCR and PCR-RFLP protocols for identifying Shiga toxins. Research in Microbiology 153, 289-300.