Gene expression profiling of individual hypothalamic nuclei from single animals using laser capture microdissection and microarrays

Journal of Neuroscience Methods

Volumn 177, Issue 1, 2009, Pages 87-93

  Paulsen, Sarah Juel ^a,b   Larsen, Leif Kongskov ^a   Jelsing, Jacob ^a   Janßen, Uwe ^c   Gerstmayer, Bernhard ^c   Vrang, Niels ^a  

^a Rheoscience A/S   (Denmark)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^c MILTENYI BIOTEC GMBH   (Germany)

Author keywords

Food deprivation; Hypothalamic arcuate nucleus; Laser capture microdissection; Microarray analysis

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; NEUROPEPTIDE Y; PROOPIOMELANOCORTIN;

ANIMAL MODEL; ANIMAL TISSUE; ARCUATE NUCLEUS; ARTICLE; CONTROLLED STUDY; DIET; DIET RESTRICTION; DNA FINGERPRINTING; DNA MICROARRAY; GENE EXPRESSION; HYPOTHALAMUS; IN SITU HYBRIDIZATION; LASER CAPTURE MICRODISSECTION; MALE; MOUSE STRAIN; NONHUMAN; NUCLEOTIDE SEQUENCE; OBESE RAT; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SENSITIVITY ANALYSIS;

ANALYSIS OF VARIANCE; ANIMALS; ARCUATE NUCLEUS; DIETARY FATS; FASTING; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; MALE; MICROARRAY ANALYSIS; MICRODISSECTION; NEUROPEPTIDE Y; OBESITY; PRO-OPIOMELANOCORTIN; RATS; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

EID: 58049220192     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2008.09.024     Document Type: Article

References (42)

1. Alexander M.J. Colocalization of neurotensin messenger ribonucleic acid (mRNA) and progesterone receptor mRNA in rat arcuate neurons under estrogen-stimulated conditions. Endocrinology 140 (1999) 4995-5003
2. Appay V., Bosio A., Lokan S., Wiencek Y., Biervert C., Kusters D., et al. Sensitive gene expression profiling of human T cell subsets reveals parallel post-thymic differentiation for CD4+ and CD8+ lineages. J Immunol 179 (2007) 7406-7414
3. Baskin D.G., Breininger J.F., and Schwartz M.W. SOCS-3 expression in leptin-sensitive neurons of the hypothalamus of fed and fasted rats. Regul Pept 92 (2000) 9-15
4. Brady L.S., Smith M.A., Gold P.W., and Herkenham M. Altered expression of hypothalamic neuropeptide mRNAs in food-restricted and food-deprived rats. Neuroendocrinology 52 (1990) 441-447
5. Cheng X., Broberger C., Tong Y., Yongtao X., Ju G., Zhang X., et al. Regulation of expression of Neuropeptide Y Y1 and Y2 receptors in the arcuate nucleus of fasted rats. Brain Res 792 (1998) 89-96
6. Dunn-Meynell A.A., Routh V.H., Kang L., Gaspers L., and Levin B.E. Glucokinase is the likely mediator of glucosensing in both glucose-excited and glucose-inhibited central neurons. Diabetes 51 (2002) 2056-2065
7. Garcia-Segura L.M., Rodriguez J.R., and Torres-Aleman I. Localization of the insulin-like growth factor I receptor in the cerebellum and hypothalamus of adult rats: an electron microscopic study. J Neurocytol 26 (1997) 479-490
8. Guan X.M., Yu H., Palyha O.C., McKee K.K., Feighner S.D., Sirinathsinghji D.J., et al. Distribution of mRNA encoding the growth hormone secretagogue receptor in brain and peripheral tissues. Brain Res Mol Brain Res 48 (1997) 23-29
9. Gundersen H.J., and Jensen E.B. The efficiency of systematic sampling in stereology and its prediction. J Microsc 147 (1987) 229-263
10. Herve C., and Fellmann D. Changes in rat melanin-concentrating hormone and dynorphin messenger ribonucleic acids induced by food deprivation. Neuropeptides 31 (1997) 237-242
11. Jureus A., Cunningham M.J., Li D., Johnson L.L., Krasnow S.M., Teklemichael D.N., et al. Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse. Endocrinology 142 (2001) 5140-5144
12. Jureus A., Cunningham M.J., McClain M.E., Clifton D.K., and Steiner R.A. Galanin-like peptide (GALP) is a target for regulation by leptin in the hypothalamus of the rat. Endocrinology 141 (2000) 2703-2706
13. Kim E.K., Miller I., Landree L.E., Borisy-Rudin F.F., Brown P., Tihan T., et al. Expression of FAS within hypothalamic neurons: a model for decreased food intake after C75 treatment. Am J Physiol Endocrinol Metab 283 (2002) E867-E879
14. Kim E.M., Welch C.C., Grace M.K., Billington C.J., and Levine A.S. Chronic food restriction and acute food deprivation decrease mRNA levels of opioid peptides in arcuate nucleus. Am J Physiol 270 (1996) R1019-R1024
15. Knudsen S. Guide to analysis of DNA microarray data. 2nd ed. (2004), John Wiley & Sons, New York
16. Kobayashi Y., Tsuchiya K., Yamanome T., Schioth H.B., Kawauchi H., and Takahashi A. Food deprivation increases the expression of melanocortin-4 receptor in the liver of barfin flounder, Verasper moseri. Gen Comp Endocrinol (2007)
17. Korner J., Savontaus E., Chua Jr. S.C., Leibel R.L., and Wardlaw S.L. Leptin regulation of Agrp and Npy mRNA in the rat hypothalamus. J Neuroendocrinol 13 (2001) 959-966
18. Kristensen P., Judge M.E., Thim L., Ribel U., Christjansen K.N., Wulff B.S., et al. Hypothalamic CART is a new anorectic peptide regulated by leptin. Nature 393 (1998) 72-76
19. Kuo W.P., Liu F., Trimarchi J., Punzo C., Lombardi M., Sarang J., et al. A sequence-oriented comparison of gene expression measurements across different hybridization-based technologies. Nat Biotechnol 24 (2006) 832-840
20. Li J.Y., Kuick R., Thompson R.C., Misek D.E., Lai Y.M., Liu Y.Q., et al. Arcuate nucleus transcriptome profiling identifies ankyrin repeat and suppressor of cytokine signalling box-containing protein 4 as a gene regulated by fasting in central nervous system feeding circuits. J Neuroendocrinol 17 (2005) 394-404
21. Li J.Y., Lescure P.A., Misek D.E., Lai Y.M., Chai B.X., Kuick R., et al. Food deprivation-induced expression of minoxidil sulfotransferase in the hypothalamus uncovered by microarray analysis. J Biol Chem 277 (2002) 9069-9076
22. Lin S., Lin E.J., Boey D., Lee N.J., Slack K., During M.J., et al. Fasting inhibits the growth and reproductive axes via distinct Y2 and Y4 receptor-mediated pathways. Endocrinology 148 (2007) 2056-2065
23. Loftus T.M., Jaworsky D.E., Frehywot G.L., Townsend C.A., Ronnett G.V., Lane M.D., et al. Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors. Science 288 (2000) 2379-2381
24. Luque R.M., Park S., and Kineman R.D. Severity of the catabolic condition differentially modulates hypothalamic expression of growth hormone-releasing hormone in the fasted mouse: potential role of Neuropeptide Y and corticotropin-releasing hormone. Endocrinology 148 (2007) 300-309
25. Lynch R.M., Tompkins L.S., Brooks H.L., Dunn-Meynell A.A., and Levin B.E. Localization of glucokinase gene expression in the rat brain. Diabetes 49 (2000) 693-700
26. Mano-Otagiri A., Nemoto T., Sekino A., Yamauchi N., Shuto Y., Sugihara H., et al. Growth hormone-releasing hormone (GHRH) neurons in the arcuate nucleus (Arc) of the hypothalamus are decreased in transgenic rats whose expression of ghrelin receptor is attenuated: Evidence that ghrelin receptor is involved in the up-regulation of GHRH expression in the arc. Endocrinology 147 (2006) 4093-4103
27. Marks J.L., Porte Jr. D., Stahl W.L., and Baskin D.G. Localization of insulin receptor mRNA in rat brain by in situ hybridization. Endocrinology 127 (1990) 3234-3236
28. Mikkelsen J.D., and Larsen P.J. A high concentration of NPY (Y1)-receptor mRNA-expressing cells in the rat arcuate nucleus. Neurosci Lett 148 (1992) 195-198
29. Morley J.E., Hernandez E.N., and Flood J.F. Neuropeptide Y increases food intake in mice. Am J Physiol 253 (1987) R516-R522
30. Morton G.J., Cummings D.E., Baskin D.G., Barsh G.S., and Schwartz M.W. Central nervous system control of food intake and body weight. Nature 443 (2006) 289-295
31. Mountjoy K.G., Mortrud M.T., Low M.J., Simerly R.B., and Cone R.D. Localization of the melanocortin-4 receptor (MC4-R) in neuroendocrine and autonomic control circuits in the brain. Mol Endocrinol 8 (1994) 1298-1308
32. Oh I.S., Shimizu H., Satoh T., Okada S., Adachi S., Inoue K., et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature 443 (2006) 709-712
33. Olchovsky D., Song J., Gelato M.C., Sherwood J., Spatola E., Bruno J.F., et al. Pituitary and hypothalamic insulin-like growth factor-I (IGF-I) and IGF-I receptor expression in food-deprived rats. Mol Cell Endocrinol 93 (1993) 193-198
34. Paulsen S.J., Christensen M.T., Vrang N., and Larsen L.K. The putative neuropeptide TAFA5 is expressed in the hypothalamic paraventricular nucleus and is regulated by dehydration. Brain Res 1199 (2008) 1-9
35. Prima V., Tennant M., Gorbatyuk O.S., Muzyczka N., Scarpace P.J., and Zolotukhin S. Differential modulation of energy balance by leptin, ciliary neurotrophic factor, and leukemia inhibitory factor gene delivery: microarray deoxyribonucleic acid-chip analysis of gene expression. Endocrinology 145 (2004) 2035-2045
36. Swanson L.W. Brain maps: structure of the rat brain: a laboratory guide with printed and electronic templates for data, models, and schematics. 2nd rev. ed. (1998), Elsevier, New York
37. Takatsu Y., Matsumoto H., Ohtaki T., Kumano S., Kitada C., Onda H., et al. Distribution of galanin-like peptide in the rat brain. Endocrinology 142 (2001) 1626-1634
38. Tups A., Helwig M., Khorooshi R.M., Archer Z.A., Klingenspor M., and Mercer J.G. Circulating ghrelin levels and central ghrelin receptor expression are elevated in response to food deprivation in a seasonal mammal (Phodopus sungorus). J Neuroendocrinol 16 (2004) 922-928
39. Tups A., Helwig M., Stohr S., Barrett P., Mercer J.G., and Klingenspor M. Photoperiodic regulation of insulin receptor mRNA and intracellular insulin signaling in the arcuate nucleus of the Siberian hamster, Phodopus sungorus. Am J Physiol Regul Integr Comp Physiol 291 (2006) R643-R650
40. Vrang N. Anatomy of hypothalamic CART neurons. Peptides 27 (2006) 1970-1980
41. Woods S.C., Seeley R.J., Porte Jr. D., and Schwartz M.W. Signals that regulate food intake and energy homeostasis. Science 280 (1998) 1378-1383
42. Xiao X.Q., Grove K.L., Lau S.Y., McWeeney S., and Smith M.S. Deoxyribonucleic acid microarray analysis of gene expression pattern in the arcuate nucleus/ventromedial nucleus of hypothalamus during lactation. Endocrinology 146 (2005) 4391-4398