Expression and characterization of antimicrobial peptide CecropinAD in the methylotrophic yeast Pichia pastoris

Process Biochemistry

Volumn 44, Issue 1, 2009, Pages 11-16

  Jin, Feng liang ^a   Xu, Xiao xia ^a   Yu, Xiao qiang ^b   Ren, Shun xiang ^a  

^a SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

^b UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

Author keywords

Anti microbial activity; Expression; Hybrid peptide; Pichia pastoris; Purification; Recursive PCR

Indexed keywords

BACTERIA; BACTERIOLOGY; CELL CULTURE; CELL MEMBRANES; COSMIC RAYS; CYTOLOGY; DICHROISM; DIFFUSERS (OPTICAL); HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHANOL; OPTICAL PROPERTIES; PURIFICATION; RECURSIVE FUNCTIONS; YEAST;

ANTI-MICROBIAL ACTIVITY; EXPRESSION; HYBRID PEPTIDE; PICHIA PASTORIS; RECURSIVE PCR;

AMINES;

BACTERIA (MICROORGANISMS); FUNGI; NEGIBACTERIA; PICHIA PASTORIS; POSIBACTERIA;

EID: 58049217417     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2008.08.012     Document Type: Article

References (38)

1. Boman H.G. Peptide antibiotics and their role in innate immunity. Ann Rev Immunol 13 (1995) 61-92
2. Brogden K.A., Ackermann M., McCray Jr. P.B., and Tack B.F. Antimicrobial peptides in animals and their role in host defences. Int J Antimicrob Agents 22 (2003) 465-478
3. Hancock R.E.W., and Chapple D.S. Peptide antibiotics. Antimicrob Agents Chemother 43 6 (1999) 1317-1323
4. Saito A., Ueda K., Imamura M., Atsumi S., Tabunoki H., Miura N., et al. Purification and cDNA cloning of a cecropin from the longicorn beetle, Acalolepta luxuriosa. Comp Biochem Physiol Part B 142 (2005) 317-323
5. Ji D., and Kim Y. An entomopathogenic bacterium, Xenorhabdus nematophila, inhibits the expression of an antibacterial peptide, cecropin, of the beet armyworm, Spodoptera exigua. J Insect Physiol 50 (2004) 489-496
6. Liang Y., Wang J.-X., Zhao X.-f., Du X.-J., and Xue J.-F. Molecular cloning and characterization of cecropin from the housefly (Musca domestica), and its expression in Escherichia coli. Dev Comp Immunol 30 (2006) 249-257
7. Morishima I., Suginaka S., Ueno T., and Hirano H. Isolation and structure of cecropins, inducible antibacterial peptides from silkworm Bomxy mori. Comp Biochem Physiol 959 (1990) 551-554
8. Kylsten P., Samakovlis C., and Hultmark D. The cecropin locus in Drosophila: a compact gene cluster involved in the response to infection. EMBO J 9 (1990) 217-224
9. Yoe S.M., Kang C.S., Han S.S., and Bang I.S. Characterization and cDNA cloning of hinnavin II, a cecropin family antibacterial peptide from the cabbage butterfly, Artogeia rapae. Comp Biochem Physiol Part B 144 (2006) 199-205
10. Xu X., Jin F., Yu X., Ji S., Wang J., Cheng H., et al. Expression and purification of a recombinant antibacterial peptide, cecropin, from Escherichia coli. Protein Expr Purif 53 (2007) 293-301
11. Choi C.S., Lee I.H., Kim E., Kim S.I., and Kim H.R. Antibacterial properties and partial cDNA sequences of cecropin-like antibacterial peptides from the common cutworm, Spodoptera litura. Comp Biochem Physiol 125 (2000) 287-297
12. Pillai A., Ueno S., Zhang H., Lee J.M., and Kato Y. Cecropin P1 and novel nematode cecropins: a bacteria-inducible antimicrobial peptide family in the nematode Ascaris suum. Biochem J 390 (2005) 207-214
13. Jin F.L., Xu X.X., Zhang W.Q., and Gu D.X. Expression and characterization of a housefly cecropin gene in the methylotrophic yeast, Pichia pastoris. Protein Expr Purif 49 (2006) 39-46
14. Wang L., Li Z., Du C., Chen W., and Pang Y. Characterization and expression of a cecropin-like gene from Helicoverpa armigera. Comp Biochem Physiol Part B 148 (2007) 417-425
15. Winder D., Günzburg W.H., Erfle V., and Salmons B. Expression of antimicrobial peptides has an antitumour effect in human cells. Biochem Biophys Res Commun 242 (1998) 608-612
16. Fink J., Merrifield R.B., Boman A., and Boman H.G. The chemical synthesis of cecropin D and analog with enhanced antibacterial activity. J Biol Chem 264 (1989) 6260-6267
17. Lee D.G., Kim P.I., Park Y., Woo E.-R., Choi J.S., Choi C.-H., et al. Design of novel peptide analogs with potent fungicidal activity, based on PMAP-23 antimicrobial peptide isolated from porcine myeloid. Biochem Biophys Res Commun 29 (2002) 231-238
18. Andreu D., Ubach J., Boman A., Wåhlin B., Wade D., Merrifield R.B., et al. Shortened cecropin A-melittin hybrids. Significant size reduction retains potent antibiotic activity. FEBS Lett 296 (1992) 190-194
19. Giacometti A., Cirioni O., Kamysz W., D'Amato G., Silvestri C., Prete M.S.D., et al. In vitro activity and killing effect of the synthetic hybrid cecropin A-melittin peptide CA(1-7)M(2-9)NH2 on methicillin-resistant nosocomial isolates of Staphylococcus aureus and interactions with clinically used antibiotics. Diagn Microb Infect Dis 49 (2004) 197-200
20. Lee D.G., Park Y., Kim P.I., Jeong H.G., Woo E.-R., and Hahm K.-S. Influence on the plasma membrane of Candida albicans by HP (2-9)-magainin 2 (1-12) hybrid peptide. Biochem Biophys Res Commun 297 (2002) 885-889
21. Park Y., Lee D.G., Jang S.H., Woo E.-R., Jeong H.G., Choi C.-H., et al. A Leu-Lys-rich antimicrobial peptide: activity and mechanism. Biochim Biophys Acta (BBA): Proteins Proteomics 1645 (2000) 172-182
22. Shin S.Y., Yang S.T., Park E.J., Eom S.H., Song W.K., Kim Y., et al. Salt resistance and synergistic effect with vancomycin of [alpha]-helical antimicrobial peptide P18. Biochem Biophys Res Commun 290 (2002) 558-562
23. Oh H.-S., Kim S., Cho H., and Lee K.-H. Development of novel lipid-peptide hybrid compounds with antibacterial activity from natural cationic antibacterial peptides. Bioorg Med Chem Lett 14 (2004) 1109-1113
24. Giacometti A., Cirioni O., Kamysz W., D'Amato G., Silvestri C., Prete M.S.D., et al. Comparative activities of cecropin A, melittin, and cecropin A-melittin peptide CA(1-7)M(2-9)NH2 against multidrug-resistant nosocomial isolates of Acinetobacter baumannii. Peptides 24 (2003) 1315-1318
25. Kwak H.B., Lee S.W., Lee D.G., Hahm K.S., Kim K.K., Kim H.H., et al. A hybrid peptide derived from cecropin-A and magainin-2 inhibits osteoclast differentiation. Life Sci 73 (2003) 993-1005
26. Shin S.Y., Kang J.H., Jang S.Y., Kim Y., Kim K.L., and Hahm K.-S. Effects of the hinge region of cecropin A(1-8)-magainin 2(1-12), a synthetic antimicrobial peptide, on liposomes, bacterial and tumor cells. Biochim Biophys Acta-Biomembr 1463 (2000) 209-218
27. Jin F.L., Xu X.X., Wang L.X., Zhang W.Q., and Gu D.X. Expression of recombinant hybrid peptide cecropinA(1-8)-magainin2(1-12) in Pichia pastoris: purification and characterization. Protein Expr Purif 50 (2006) 147-156
28. Cereghino J.L., and Cregg J.M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24 (2000) 45-66
29. Sreekrishna K., Brankamp R.G., Kropp K.E., Blankenship D.T., Tsay J.T., Smith P.L., et al. Strategies for optimal synthesis and secretion of heterologous protein in the methylotrophic yeast Pichia pastoris. Gene 190 (1997) 55-62
30. Hultmark D., Engstrom A., and Bennich H. Insect immunity: isolation and structure of cecropin and four minor antibacterial components from cecropia pupa. Eur J Biochem 127 (1982) 207-217
31. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., et al. Protocols in molecular biology (3rd ed.). Trends Cell Biol 6 (1996) 366-367
32. Lehrer R.I., Rosenman M., Harwig S.S.L., Jackson R., and Eisenhauer P. Ultrasensitive assays for endogenous antimicrobial polypeptides. J Immunol Methods 137 (1990) 167-173
33. Bulet P., Stöcklin R., and Menin L. Anti-microbial peptides: from invertebrates to vertebrates. Immunol Rev 198 (2004) 169-184
34. Jang W.S., Kim H.K., Lee K.Y., Kim S.A., Han Y.S., and Lee I.H. Antifungal activity of synthetic peptide derived from halocidin, antimicrobial peptide from the tunicate, Halocynthia aurantium. FEBS Lett 580 (2006) 1490-1496
35. Sung W.S., Lee J., and Lee D.G. Fungicidal effect and the mode of action of piscidin 2 derived from hybrid striped bass. Biochem Biophys Res Commun 371 (2008) 551-555
36. Epand R.F., Mowery B.P., Lee S.E., Stahl S.S., Lehrer R.I., Gellman S.H., et al. Dual mechanism of bacterial lethality for a cationic sequence-random copolymer that mimics host-defense antimicrobial peptides. J Mol Biol 379 (2008) 38-50
37. Shlyapnikov Y.M., Andreev Y.A., Kozlov S.A., Vassilevski A.A., and Grishin E.V. Bacterial production of latarcin 2a, a potent antimicrobial peptide from spider venom. Protein Expr Purif 60 (2008) 89-95
38. Hoskin D.W., and Ramamoorthy A. Studies on anticancer activities of antimicrobial peptides. Biochim Biophys Acta 1778 (2008) 357-375