Fractionation and characterization of chitosan by analytical SEC and 1H NMR after semi-preparative SEC

Carbohydrate Polymers

Volumn 75, Issue 4, 2009, Pages 636-645

  Nguyen, Sophie ^a,b   Hisiger, Steve ^a   Jolicoeur, Mario ^a   Winnik, Françoise M ^b   Buschmann, Michael D ^a  

^a ÉCOLE POLYTECHNIQUE DE MONTRÉAL   (Canada)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Chitosan; Degree of deacetylation; Fractionation; Molecular weight; SEC

Indexed keywords

ACETYLATION; AMINES; CHITIN; CHITOSAN; HYDROGELS; INORGANIC ACIDS; LIGHT SCATTERING; MOLECULAR WEIGHT; MULTILAYERS; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; WEIGHING;

COMPOSITIONAL HETEROGENEITIES; DEGREE OF DEACETYLATION; LASER LIGHT SCATTERINGS; LOW MOLECULAR WEIGHT CHITOSANS; MONODISPERSE; NITROUS ACIDS; PREPARATION METHODS; SEC;

BIOLOGICAL MEMBRANES;

EID: 57849167417     PISSN: 01448617     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.carbpol.2008.09.002     Document Type: Article

References (48)

1. Anthonsen M.W., Varum K.M., Hermansson A.M., Smidsrod O., and Brant D.A. Aggregates in acidic solutions of chitosans detected by static laser light scattering. Carbohydrate Polymer 25 (1994) 13-23
2. Bahrke S., Einarsson J.M., Gislason J., Haebel S., Letzel M.C., Peter-Katalinić J., et al. Sequence analysis of chitooligosaccharides by matrix-assisted laser desorption ionization postsource decay mass spectrometry. Biomacromolecules 3 (2002) 696-704
3. Barth H.G. A Practical Approach to Steric Exclusion Chromatography of Water-Soluble Polymers. Journal of Chromatographic Science 18 (1980) 409-429
4. Beri R.G., Walker J., Reese E.T., and Rollings J.E. Characterization of chitosans via coupled size-exclusion chromatography and multiple-angle laser light-scattering technique. Carbohydrate Research 238 (1993) 11-26
5. Berth G., and Dautzenberg H. The degree of acetylation of chitosans and its effect on the chain conformation in aqueous solution. Carbohydrate Polymer 47 (2002) 39-51
6. Berth G., Dautzenberg H., and Peter M.G. Physico-chemical characterization of chitosans varying in degree of acetylation. Carbohydrate Polymer 36 (1998) 205-216
7. Borchard G. Chitosans for gene delivery. Advanced Drug Delivery Reviews 52 (2001) 145-150
8. Chance R.R., Baniukiewicz S.P., Mintz D., and Ver Strate G. Characterization of low-molecular-weight polymers: Failure of universal calibration in size exclusion chromatography. International Journal of Polymer Analysis and Characterization 1 (1995) 3-34
9. Chatelet C., Damour O., and Domard A. Influence of the degree of acetylation on some biological properties of chitosan films. Biomaterials 22 (2001) 261-268
10. Chevrier A., Hoemann C.D., Sun J., and Buschmann M.D. Chitosan-glycerol phosphate/blood implants increase cell recruitment, transient vascularisation and subchondral bone remodeling in drilled cartilage defects. Osteoarthritis and Cartilage 15 (2007) 316-327
11. Christensen, B. E., 2007, Mixing polydisperse samples, personal communication.
12. Denuzière A., Yagoubi N., Baillet A., and Ferrier D. An improved statistical parameter allowing elaborate comparison of polymer molecular weight distribution by gel filtration chromatography: Application to chitosan. S.T.P. Pharma Sci. 5 (1995) 481-485
13. Di Martino A., Sittinger M., and Risbud M.V. Chitosan: A versatile biopolymer for orthopaedic tissue-engineering. Biomaterials 26 (2005) 5983-5990
14. Fee M., Errington N., Jumel K., Illum L., Smith A., and Harding S.E. Correlation of SEC/MALLS with ultracentrifuge and viscometric data for chitosans. European Biophysics Journal 32 (2003) 457-464
15. Hoemann C.D., Hurtig M., Rossomacha E., Sun J., Chevrier A., Shive M.S., et al. Chitosan-glycerol phosphate/blood implants improve hyaline cartilage repair in ovine microfracture defects. Journal of Bone and Joint Surgery 87 (2005) 2671-2686
16. Hoemann C.D., Sun J., Légaré A., McKee M.D., and Buschmann M.D. Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle. Osteoarthritis and Cartilage 13 (2005) 318-329
17. Hoemann C.D., Sun J., McKee M.D., Chevrier A., Rossomacha E., Rivard G.-E., et al. Chitosan-glycerol phosphate/blood implants elicit hyaline cartilage repair integrated with porous subchondral bone in microdrilled rabbit defects. Osteoarthritis and Cartilage 15 (2007) 78-89
18. Itakura M., Sato K., Lusenkova M.A., Matsuyama S., Shimada K., Saito T., et al. Molecular weight dependency of refractive index increment of polystyrene determined by uniform oligomers. Journal of Applied Polymer Science 94 (2004) 1101-1106
19. Khor E. Chitin: A biomaterial in waiting. Current Opinion in Solid State and Materials Science 6 (2002) 313-317
20. Knaul J.Z., Kasaai M.R., Bui V.T., and Creber K.A.M. Characterization of deacetylated chitosan and chitosan molecular weight review. Canadian Journal of Chemistry 76 (1998) 1699-1706
21. Koide S.S. Chitin-chitosan: Properties, benefits and risks. Nutrition Research 18 (1998) 1091-1101
22. Köping-Höggård M., Mel'nikova Y.S., Varum K.M., Lindman B., and Artursson P. Relationship between the physical shape and the efficiency of oligomeric chitosan as a gene delivery system in vitro and in vivo. The Journal of Gene Medicine 5 (2003) 130-141
23. Köping-Höggård M., Tubulekas I., Guan H., Edwards K., Nilsson M., Varum K.M., et al. Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo. Gene Therapy 8 (2001) 1108-1121
24. Köping-Höggård M., Varum K.M., Issa M., Danielsen S., Christensen B.E., Stokke B.T., et al. Improved chitosan-mediated gene delivery based on easily dissociated chitosan polyplexes of highly defined chitosan oligomers. Gene Therapy 11 (2004) 1441-1452
25. Kubota N., Tatsumoto N., Sano T., and Toya K. A simple preparation of half N-acetylated chitosan highly soluble in water and aqueous organic solvents. Carbohydrate Research 324 (2000) 268-274
26. Kujawa P., Moraille P., Sanchez J., Badia A., and Winnik F.M. Effect of molecular weight on the exponential growth and morphology of hyaluronan/chitosan multilayers: A surface plasmon resonance spectroscopy and atomic force microscopy investigation. Journal of the American Chemical Society 127 (2005) 9224-9234
27. Kumar M.N.V.R., Muzzarelli R.A.A., Muzzarelli C., Sashiwa H., and Domb A.J. Chitosan chemistry and pharmaceutical perspectives. Chemical Reviews 104 (2004) 6017-6084
28. Lamarque G., Lucas J.-M., Viton C., and Domard A. Physicochemical behavior of homogeneous series of acetylated chitosans in aqueous solution: Role of various structural parameters. Biomacromolecules 6 (2005) 131-142
29. Lavertu M., Méthot S., Tran-Khanh N., and Buschmann M.D. High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation. Biomaterials 27 (2006) 4815-4824
30. 1H NMR method for the determination of the degree of deacetylation of chitosan. Journal of Pharmaceutical and Biomedical Analysis 32 (2003) 1149-1158
31. Li H., Du Y., and Xu Y. Adsorption and complexation of chitosan wet-end additives in papermaking systems. Journal of Applied Polymer Science 91 (2004) 2642-2648
32. Liu H., Du Y., Wang X., Hu Y., and Kennedy J.F. Interaction between chitosan and alkyl β-d-glucopyranoside and its effect on their antimicrobial activity. Carbohydrate Polymer 56 (2004) 243-250
33. Liu W.G., and Yao K.D. Chitosan and its derivatives - A promising non-viral vector for gene transfection. Journal of Controlled Release 83 (2002) 1-11
34. Mao H.-Q., Roy K., Vu L.T.-L., Janes K.A., Lin K.Y., Wang Y., et al. Chitosan-DNA nanoparticles as gene carriers: Synthesis, characterization and transfection efficiency. Journal of Controlled Release 70 (2001) 399-421
35. Muzzarelli R.A.A., Xia W., Tomasetti M., and Ilari P. Depolymerization of chitosan and substituted chitosans with the aid of a wheat germ lipase preparation. Enzyme and Microbial Technology 17 (1995) 541-545
36. Nguyen S., Winnik F.M., and Buschmann M.D. Improved reproducibility in the determination of the molecular weight of chitosan by analytical size exclusion chromatography. Carbohydrate Polymers 75 (2009) 528-533
37. Ottoy M.H., Varum K.M., Christensen B.E., Anthonsen M.W., and Smidsrod O. Preparative and analytical size-exclusion chromatography of chitosans. Carbohydrate Polymer 31 (1996) 253-261
38. Ottoy M.H., Varum K.M., and Smidsrod O. Compositional heterogeneity of heterogeneously deacetylated chitosans. Carbohydrate Polymer 29 (1996) 17-24
39. Philippova O.E., Volkov E.V., Sitnikova N.L., and Khokhlov A.R. Two types of hydrophobic aggregates in aqueous solutions of chitosan and its hydrophobic derivative. Biomacromolecules 2 (2001) 483-490
40. Podešva J., Procházka O., and Medin A. Studies on agaroses: 1. Specific refractive index increments in dimethyl sulfoxide and in water at various wavelengths and temperatures. Polymer 36 (1995) 4967-4970
41. Schatz C., Viton C., Delair T., Pichot C., and Domard A. Typical physicochemical behaviors of chitosan in aqueous solution. Biomacromolecules 4 (2003) 641-648
42. Sorlier P., Denuzière A., Viton C., and Domard A. Relation between the degree of acetylation and the electrostatic properties of chitin and chitosan. Biomacromolecules 2 (2001) 765-772
43. Tommeraas K., Köping-Höggård M., Varum K.M., Christensen B.E., Artursson P., and Smidsrod O. Preparation and characterisation of chitosans with oligosaccharide branches. Carbohydrate Research 337 (2002) 2455-2462
44. Tommeraas K., Varum K.M., Christensen B.E., and Smidsrod O. Preparation and characterization of oligosaccharides produced by nitrous acid depolymerisation of chitosans. Carbohydrate Research 333 (2001) 137-144
45. Tosoh Bioscience. Size Exclusion Chromatography, TSKgel PW-type: PW and PWXL.***
46. Tsaih M.L., and Chen R.H. Molecular weight determination of 83% degree of deacetylation chitosan with non-gaussian and wide range distribution by high-performance size exclusion chromatography and capillary viscometry. Journal of Applied Polymer Science 71 (1999) 1905-1913
47. Varum K.M., Ottøy M.H., and Smidsrod O. Acid hydrolysis of chitosans. Carbohydrate Polymers 46 (2001) 89-98
48. Wyatt P.J. Light scattering and the absolute characterization of macromolecules. Analytica Chimica Acta 272 (1993) 1-40