Correlation of SEC/MALLS with ultracentrifuge and viscometric data for chitosans

European Biophysics Journal

Volumn 32, Issue 5, 2003, Pages 457-464

  Fee, Monica ^a   Errington, Neil ^a   Jumel, Kornelia ^a   Illum, Lisbeth ^b   Smith, Alan ^b   Harding, Stephen E ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b Drug Delivery and Clinical Research Centre Ltd   (United Kingdom)

Author keywords

Chitosan; Conformation; Molecular weight; SEC MALLS; Sedimentation equilibrium

Indexed keywords

CHITOSAN;

ACETYLATION; CONFERENCE PAPER; CORRELATION ANALYSIS; GEL PERMEATION CHROMATOGRAPHY; LASER; LIGHT SCATTERING; MOLECULAR WEIGHT; SEDIMENTATION; ULTRACENTRIFUGATION; VISCOSITY;

CENTRIFUGATION, DENSITY GRADIENT; CHITIN; CHITOSAN; CHROMATOGRAPHY, GEL; LASERS; MOLECULAR WEIGHT; NEPHELOMETRY AND TURBIDIMETRY; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; STATISTICS; ULTRACENTRIFUGATION; VISCOSITY;

EID: 0042860018     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00249-003-0317-8     Document Type: Conference Paper

References (46)

1. Anthonsen MW, Vårum KM, Smidsrød O (1993) Solution properties of chitosans: conformation and chain stiffness of chitosans with different degrees of N-acetylation. Carbohydr Polym 22:193-201
2. Anthonsen MW, Vårum KM, Hermansson AM, Smidsrød O, Brant DA (1994) Aggregates in acidic solutions of chitosans detected by static laser light scattering. Carbohydr Polym 25:13-23
3. Artursson P, Lindmark T, Davis SS, Illum L (1994) Effect of chitosan on the permeability of monolayers of intestinal epithelial cells (caco-2). Pharm Res 11:1358-1361
4. Beri RG, Walker J, Reese ET, Rollings JE (1993) Characterization of chitosans via coupled size-exclusion chromatography and multiple-angle laser light-scattering technique. Carbohydr Res 238:11-26
5. Berth G, Dautzenberg H (2002) The degree of acetylation of chitosans and its effect on the chain conformation in aqueous solution. Carbohydr Polym 47:39-51
6. Berth G, Dautzenberg H, Peter MG (1998) Physico-chemical characterization of chitosans varying in degree of acetylation. Carbohydr Polym 36:205-216
7. Clewlow AC, Errington N, Rowe AJ (1997) Analysis of data captured by an on-line image capture system from an analytical ultracentrifuge using Schlieren optics. Eur J Biophys 25:311-317
8. Cölfen H, Harding SE (1997) MSTARA and MSTARI: interactive PC algorithms for simple, model independent evaluation of sedimentation equilibrium data. Eur J Biophys 25:333-346
9. Cölfen H, Berth G, Dautzenberg H (2001) Hydrodynamic studies on chitosans in aqueous solution. Carbohydr Polym 45:373-383
10. Davis SS (2001) Nasal vaccines. Adv Drug Delivery Rev 51:21-42
11. Deacon MP, Davis SS, White RJ, Nordman H, Carlstedt I, Errington N, Rowe AJ, Harding SE (1999) Are chitosan-mucin interactions specific to different regions of the stomach? Velocity ultracentrifugation offers a clue. Carbohydr Polym 38:235-238
12. Errington N, Harding SE, Vårum KM, Illum L (1993) Hydrodynamic characterisation of chitosans varying in degree of acetylation. Int J Biol Macromol 15:113-117
13. Fujita H (1962) Mathematical theory of sedimentation analysis. Academic Press, New York, p 297
14. Harding SE (1992) Sedimentation analysis of polysaccharides. In: Harding SE, Rowe AJ, Horton JC (eds) Analytical ultracentrifugation in biochemistry and polymer science. Royal Society of Chemistry, Cambridge, UK
15. Harding SE (1995a) On the hydrodynamic analysis of macromolecular conformation. Biophys Chem 55:69-93
16. Harding SE (1995b) Some recent developments in the size and shape analysis of industrial polysaccharides in solution using sedimentation analysis in the analytical ultracentrifuge. Carbohydr Polym 28:227-237
17. Harding SE (1997) The intrinsic viscosity of biological macromolecules. Progress in measurement, interpretation and application to structure in dilute solution. Prog Biophys Mol Biol 68:207-262
18. Harding SE, Davis SS, Deacon MP, Fiebrig I (1999) Biopolymer mucoadhesives. Biotechnol Genet Eng Rev 16:41-85
19. Horton JC, Harding SE, Mitchell JR (1991) Gel permeation chromatography-multi angle laser light scattering characterisation of the molecular mass distribution of "Pro-nova" sodium alginate. Biochem Soc Trans 19:510-511
20. Illum L (1998) Chitosan and its use as a pharmaceutical excipient. Pharm Res 15:1326-1331
21. Illum L, Davis SS (2001) Nasal vaccination: a non-invasive vaccine delivery method that holds great promise for the future. Adv Drug Delivery Rev 51:1-3
22. Illum L, Farraj NF, Davis SS (1994) Chitosans as a novel nasal delivery system for peptide drugs. Pharm Res 11:1186-1189
23. Illum L, Jabbal-Gill I, Hinchcliffe M, Fisher AN, Davis SS (2001) Chitosan as a novel nasal delivery system for vaccines. Adv Drug Delivery Rev 51:81-96
24. Jumel K, Fiebrig I, Harding SE (1996) Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography/multi angle laser light scattering. Int J Biol Macromol 18:133-139
25. Jumel K, Fogg FJJ, Hutton DA, Pearson JP, Allen A, Harding SE (1997) A polydisperse linear random coil model for the quaternary structure of pig colonic mucin. Eur Biophys J 25:477-480
26. Kotze AF, de Leeuw BJ, Luessen HL, de Boer AG, Verhoef JC, Junginger HE (1997) Chitosans for enhanced delivery of therapeutic peptides across intestinal epithelia: in vitro evaluation in Caco-2 cell monolayers. Int J Pharm 159:243-253
27. Kratky D, Leopold A, Stabinger H (1973) The determination of the partial specific volume of proteins by the mechanical oscillator technique. Methods Enzymol 27D:98-110
28. Natsume H, Iwata S, Ohtake K, Miyamoto M, Yamaguchi M, Hosoya K, Kobayashi D, Sugibayashi K, Morimoto Y (1999) Screening of cationic compounds as an absorption enhancer for nasal drug delivery. Int J Pharm 185:1-12
29. Ottoy MH, Virum KM, Smidsrod O (1996) Compositional heterogeneity of heterogeneously deacetylated chitosans. Carbohydr Polym 29:17-24
30. Pavlov GM, Rowe AJ, Harding SE (1997) Conformation zoning of large molecules using the analytical ultracentrifuge. Trends Anal Chem 16:401-405
31. Pavlov GM, Harding SE, Rowe AJ (1999) Normalized scaling relations as a natural classification of linear macromolecules according to size. Prog Colloid Polym Sci 113:76-80
32. Philo JS (2000) A method for directly fitting the time derivative of sedimentation velocity data and an alternative algorithm for calculating sedimentation coefficient distribution functions. Anal Biochem 279:151-163
33. Rollings JE (1991) Use of online laser light-scattering photometry coupled to chromatographic separations for determination of biopolymer molecular-mass, branching, size and shape distributions. Biochem Soc Trans 19:493-494
34. Rollings JE (1992) Use of on-line laser light scattering coupled to chromatographic separations for the determination of molecular weight, branching, size and shape distributions of polysaccharides. In: Harding SE, Sattelle DB, Bloomfield VA (eds) Laser light scattering in biochemistry. Royal Society of Chemistry, Cambridge, UK
35. Schachman HK (1959) Ultracentrifugation in biochemistry. Academic Press, New York
36. Schipper NGM, Vårum KM, Artursson P (1996) Chitosans as absorption enhancers for poorly absorbable drugs. 1. Influence of molecular weight and degree of acetylation on drug transport across human intestinal epithelium (Caco-2) cells. Pharm Res 13:1686-1692
37. Skaugrud Ø, Hagen A, Borgersen B, Dornish M (1999) Biomedical and pharmaceutical applications of alginate and chitosan. Biotechnol Genet Eng Rev 16:23-40
38. Smidsrød O, Andresen L (1979) Biopolymerkjemi. Tapir, Trondheim, Norway
39. Tanford C (1955) Intrinsic viscosity and kinematic viscosity. J Phys Chem 59:798-799
40. Tengamnuay P, Sahamethapat A, Sailasuta A, Mitra AK (2000) Chitosans as nasal absorption enhancers of peptides: comparison between free amine chitosans and soluble salts. Int J Pharm 197:53-67
41. Tombs MP, Harding SE (1998) An introduction to polysaccharide biotechnology. Taylor and Francis, London, p 15
42. Van Holde KE (1998) Principles of physical biochemistry. Prentice-Hall, Englewood Cliffs, NJ, USA
43. Vårum KM, Anthonsen MW, Grasdalen H, Smidsrød O (1991) High-field NMR spectroscopy of partially N-deacetylated chitins (chitosans). 1. Determination of the degree of N-acetylation and the distribution of N-acetyl groups in partially N-deacetylated chitins. Carbohydr Res 211:17-23
44. Wang W, Bo S, Li S, Qin W (1991) Determination of the Mark-Houwink equation for chitosan with different degrees of deacetylation. Int J Biol Macromol 13:281-285
45. Wyatt PJ (1992) Combined differential light scattering with various liquid chromatography separation techniques. In: Harding SE, Sattelle DB, Bloomfield VA (eds) Laser light scattering in biochemistry. Royal Society of Chemistry, Cambridge, UK, pp 35-58
46. Wyatt PJ (1993) Light scattering and the absolute characterization of macromolecules. Anal Chim Acta 272:1-40