Structural evidence for common ancestry of the nuclear pore complex and vesicle coats

Science

Volumn 322, Issue 5906, 2008, Pages 1369-1373

  Brohawn, Stephen G ^a   Leksa, Nina C ^a   Spear, Eric D ^a   Rajashankar, Kanagalaghatta R ^b   Schwartz, Thomas U ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEOPORIN; NUCLEOPORIN NUP84; NUCLEOPORIN NUP85; UNCLASSIFIED DRUG;

ANCESTRY; BIOCHEMISTRY; COEVOLUTION; CYTOPLASM; GENETIC ANALYSIS; PROTEIN;

ARTICLE; COMPLEX FORMATION; CRYSTAL STRUCTURE; MACROMOLECULE; MEMBRANE TRANSPORT; MEMBRANE VESICLE; NUCLEAR PORE COMPLEX; NUCLEOCYTOPLASMIC TRANSPORT; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; BINDING SITES; COATED VESICLES; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; EVOLUTION, MOLECULAR; MEMBRANE PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NUCLEAR PORE; NUCLEAR PORE COMPLEX PROTEINS; NUCLEAR PROTEINS; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; SACCHAROMYCES CEREVISIAE PROTEINS; VESICULAR TRANSPORT PROTEINS;

EID: 57149118232     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1165886     Document Type: Article

References (41)

1. E. J. Tran, S. R. Wente, Cell 125, 1041 (2006).
2. K. Weis, Cell 112, 441 (2003).
3. M. A. D'Angelo, M. W. Hetzer, Trends Cell Biol. 18, 456 (2008).
4. M. Beck, V. Lucic, F. Forster, W. Baumeister, O. Medalia, Nature 449, 611 (2007).
5. S. P. Drummond, S. A. Rutherford, H. S. Sanderson, T. D. Allen, Can. J. Physiol. Pharmacol. 84, 423 (2006).
6. D. Stoffler et al., J. Mol. Biol. 328, 119 (2003).
7. I. C. Berke, T. Boehmer, G. Blobel, T. U. Schwartz, J. Cell Biol. 167, 591 (2004).
8. T. Boehmer, S. Jeudy, I. C. Berke, T. U. Schwartz, Mol. Cell 30, 721 (2008).
9. K. C. Hsia, P. Stavropoulos, G. Blobel, A. Hoelz, Cell 131, 1313 (2007).
10. S. Jeudy, T. U. Schwartz, J. Biol. Chem. 282, 34904 (2007).
11. N. Pante, M. Kann, Mol. Biol. Cell 13, 425 (2002).
12. T. U. Schwartz, Curr. Opin. Struct. Biol. 15, 221 (2005).
13. G. Rabut, V. Doye, J. Ellenberg, Nat. Cell Biol. 6, 1114 (2004).
14. M. Lutzmann, R. Kunze, A. Buerer, U. Aebi, E. Hurt, EMBO J. 21, 387 (2002).
15. E. Fabre, E. Hurt, Annu. Rev. Genet. 31, 277 (1997).
16. V. Galy, I. W. Mattaj, P. Askjaer, Mol. Biol. Cell 14, 5104 (2003).
17. A. Harel et al., Mol. Cell 11, 853 (2003).
18. C. P. Lusk, T. Makhnevych, M. Marelli, J. D. Aitchison, R. W. Wozniak, J. Cell Biol. 159, 267 (2002).
19. M. Marelli, J. D. Aitchison, R. W. Wozniak, J. Cell Biol. 143, 1813 (1998).
20. U. Zabel et al., J. Cell Biol. 133, 1141 (1996).
21. D. Devos et al., Proc. Natl. Acad. Sci. U.S.A. 103, 2172 (2006).
22. D. Devos et al., PLoS Biol. 2, e380 (2004).
23. F. Alber et al., Nature 450, 695 (2007).
24. I. Chaudhuri, J. Soding, A. N. Lupas, Proteins 71, 795 (2008).
25. J. Garcia De La Torre, M. L. Huertas, B. Carrasco, Biophys. J. 78, 719 (2000).
26. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
27. V. Doye, R. Wepf, E. C. Hurt, EMBO J. 13, 6062 (1994).
28. S. Siniossoglou et al., Cell 84, 265 (1996).
29. G. Drin et al., Nat. Struct. Mol. Biol. 14, 138 (2007).
30. P. Grandi, N. Schlaich, H. Tekotte, E. C. Hurt, EMBO J. 14, 76 (1995).
31. S. Fath, J. D. Mancias, X. Bi, J. Goldberg, Cell 129, 1325 (2007).
32. R. M. Bennett-Lovsey, A. D. Herbert, M. J. Sternberg, L. A. Kelley, Proteins 70, 611 (2008).
33. N. Schrader et al., Mol. Cell 29, 46 (2008).
34. E. Conti, C. W. Muller, M. Stewart, Curr. Opin. Struct. Biol. 16, 237 (2006).
35. A. Fotin et al., Nature 432, 573 (2004).
36. D. J. Owen, B. M. Collins, P. R. Evans, Annu. Rev. Cell Dev. Biol. 20, 153 (2004).
37. S. M. Stagg et al., Cell 134, 474 (2008).
38. Y. Cheng, W. Boll, T. Kirchhausen, S. C. Harrison, T. Walz, J. Mol. Biol. 365, 892 (2007).
39. J. E. Hinshaw, R. A. Milligan, J. Struct. Biol. 141, 259 (2003).
40. B. Dujon, Trends Genet. 22, 375 (2006).
41. We thank staff at beamlines 24-ID-C/-E at Argonne National Laboratory and X6A at National Synchrotron Light Source for excellent assistance with data collection, R. Sauer and T. Baker for critically reading the manuscript, G. Wink for contributions, members of the Schwartz laboratory for discussions, and the Biophysical Instrumentation Facility for the Study of Complex Macromolecular Systems (NSF-0070319 and NIH GM68762) for providing instrumentation. Supported by NIH grant GM77537 (T.U.S.), a Pew Scholar Award (T.U.S.), a Koch Fellowship Award (S.G.B.), and a Vertex Scholarship (S.G.B.). Coordinates and structure factors of the Nup85•Seh1 crystal structure have been deposited in the Protein Data Bank (PDB) with accession code 3EWE.