Caenorhabditis elegans Nucleoporins Nup93 and Nup205 Determine the Limit of Nuclear Pore Complex Size Exclusion in Vivo

Molecular Biology of the Cell

Volumn 14, Issue 12, 2003, Pages 5104-5115

  Galy, Vincent ^a   Mattaj, Iain W ^a   Askjaer, Peter ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

HELMINTH PROTEIN; NUCLEAR PROTEIN; NUCLEOPORIN; NUCLEOPORIN 205; NUCLEOPORIN 93; RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CAENORHABDITIS ELEGANS; CELL NUCLEUS MEMBRANE; CELL VIABILITY; CHROMATIN CONDENSATION; CONTROLLED STUDY; EMBRYO; EMBRYO DEVELOPMENT; FEMALE; IN VIVO STUDY; MACROMOLECULE; NONHUMAN; NUCLEAR PORE COMPLEX; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN FUNCTION; SEQUENCE HOMOLOGY; VERTEBRATE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CAENORHABDITIS ELEGANS; FLUORESCENT ANTIBODY TECHNIQUE; NUCLEAR ENVELOPE; NUCLEAR PORE; NUCLEAR PORE COMPLEX PROTEINS; RNA, SMALL INTERFERING; XENOPUS PROTEINS;

ANIMALIA; CAENORHABDITIS; CAENORHABDITIS ELEGANS; NEMATODA; VERMES; VERTEBRATA;

EID: 0344875469     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E03-04-0237     Document Type: Article

References (51)

1. Antonny, B., and Schekman, R. (2001). ER export: public transportation by the COPII coach. Curr. Opin. Cell Biol. 13, 438-443.
2. Askjaer, P., Galy, V., Hannak, E., and Mattaj, I.W. (2002). Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos. Mol. Biol. Cell 13, 4355-4370.
3. Bagley, S., Goldberg, M.W., Cronshaw, J.M., Rutherford, S., and Allen, T.D. (2000). The nuclear pore complex. J. Cell Sci. 113, 3885-3886.
4. Belgareh, N., et al. (2001). An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells. J. Cell Biol. 154, 1147-1160.
5. Boehmer, T., Enninga, J., Dales, S., Blobel, G., and Zhong, H. (2003). Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex. Proc. Natl. Acad. Sci. USA 100, 981-985.
6. Cohen, M., Tzur, Y.B., Neufeld, E., Feinstein, N., Delannoy, M.R., Wilson, K.L., and Gruenbaum, Y. (2002). Transmission electron microscope studies of the nuclear envelope in Caenorhabditis elegans embryos. J. Struct. Biol. 140, 232-240.
7. Cohen, M., Feinstein, N., Wilson, K.L., and Gruenbaum, Y. (2003). Nuclear pore protein gp210 is essential for viability in HeLa cells and C. elegans. Mol. Biol. Cell 14, 4230-4237.
8. Colaiacovo, M.P., Stanfield, G.M., Reddy, K.C., Reinke, V., Kim, S.K., and Villeneuve, A.M. (2002). A targeted RNAi screen for genes involved in chromosome morphogenesis and nuclear organization in the Caenorhabditis elegans germline. Genetics 162, 113-128.
9. Conti, E., and Izaurralde, E. (2001). Nucleocytoplasmic transport enters the atomic age. Curr. Opin. Cell Biol. 13, 310-319.
10. Cronshaw, J.M., Krutchinsky, A.N., Zhang, W., Chait, B.T., and Matunis, M.J. (2002). Proteomic analysis of the mammalian nuclear pore complex. J. Cell Biol. 158, 915-927.
11. Davis, L.I., and Blobel, G. (1987). Nuclear pore complex contains a family of glycoproteins that includes p 62, glycosylation through a previously unidentified cellular pathway. Proc. Natl. Acad. Sci. USA 84, 7552-7556.
12. Doye, V., and Hurt, E. (1997). From nucleoporins to nuclear pore complexes. Curr. Opin. Cell Biol. 9, 401-411.
13. Fabre, E., and Hurt, E. (1997). Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu. Rev. Genet. 31, 277-313.
14. Feldherr, C.M., and Akin, D. (1990). The permeability of the nuclear envelope in dividing and nondividing cell cultures. J. Cell Biol. 111, 1-8.
15. Feuerbach, F., Galy, V., Trelles-Sticken, E., Fromont-Racine, M., Jacquier, A., Gilson, E., Olivo-Marin, J.C., Scherthan, H., and Nehrbass, U. (2002). Nuclear architecture and spatial positioning help establish transcriptional states of telomeres in yeast. Nat. Cell Biol. 4, 214-221.
16. Fontoura, B.M., Blobel, G., and Matunis, M.J. (1999). A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96. J. Cell Biol. 144, 1097-1112.
17. Fraser, A.G., Kamath, R.S., Zipperlen, P., Martinez-Campos, M., Sohrmann, M., and Ahringer, J. (2000). Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature 408, 325-330.
18. Gomez-Ospina, N., Morgan, G., Giddings, T.H., Jr., Kosova, B., Hurt, E., and Winey, M. (2000). Yeast nuclear pore complex assembly defects determined by nuclear envelope reconstruction. J. Struct. Biol. 132, 1-5.
19. Gönczy, P., et al. (2000). Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III. Nature 408, 331-336.
20. Görlich, D., and Kutay, U. (1999). Transport between the cell nucleus and the cytoplasm. Annu. Rev. Cell Dev. Biol. 15, 607-660.
21. Grandi, P., Dang, T., Pane, N., Shevchenko, A., Mann, M., Forbes, D., and Hurt, E. (1997). Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly. Mol. Biol. Cell 8, 2017-2038.
22. Harel, A., Orjalo, A.V., Vincent, T., Lachish-Zalait, A., Vasu, S., Shah, S., Zimmerman, E., Elbaum, M., and Forbes, D.J. (2003). Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores. Mol. Cell 11, 853-864.
23. Jessberger, R. (2002). The many functions of SMC proteins in chromosome dynamics. Nat. Rev. Mol. Cell. Biol. 3, 767-778.
24. Kamath, R.S., et al. (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 421, 231-237.
25. Kerscher, O., Hieter, P., Winey, M., and Basrai, M.A. (2001). Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation. Genetics 157, 1543-1553.
26. Kosova, B., Pante, N., Rollenhagen, C., and Hurt, E. (1999). Nup192p is a conserved nucleoporin with a preferential location at the inner site of the nuclear membrane. J. Biol. Chem. 274, 22646-22651.
27. Lenart, P., Rabut, G., Daigle, N., Hand, A.R., Terasaki, M., and Ellenberg, J. (2003). Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes. J. Cell Biol. 160, 1055-1068.
28. Lin, F., Blake, D.L., Callebaut, I., Skerjanc, I.S., Holmer, L., McBurney, M.W., Paulin-Levasseur, M., and Worman, H.J. (2000). MAN1, an inner nuclear membrane protein that shares the LEM domain with lamina-associated polypeptide 2 and emerin. J. Biol. Chem. 275, 4840-4847.
29. Loewinger, L., and McKeon, F. (1988). Mutations in the nuclear lamin proteins resulting in their aberrant assembly in the cytoplasm. EMBO J. 7, 2301-2309.
30. Maeda, I., Kohara, Y., Yamamoto, M., and Sugimoto, A. (2001). Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Curr. Biol. 11, 171-176.
31. Maduro, M., and Pilgrim, D. (1995). Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system. Genetics 141, 977-988.
32. Mattaj, I.W., and Englmeier, L. (1998). Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem. 67, 265-306.
33. Miller, D.M., Desai, N.S., Hardin, D.C., Piston, D.W., Patterson, G.H., Fleenor, J., Xu, S., and Fire, A. (1999). Two-color GFP expression system for C. elegans. Biotechniques 26, 914-921.
34. Miller, B.R., Powers, M., Park, M., Fischer, W., and Forbes, D.J. (2000). Identification of a new vertebrate nucleoporin, Nup188, with the use of a novel organelle trap assay. Mol. Biol. Cell 11, 3381-3396.
35. Nehrbass, U., Rout, M.P., Maguire, S., Blobel, G., and Wozniak, R.W. (1996). The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex. J. Cell Biol. 133, 1153-1162.
36. Piano, F., Schetter, A.J., Morton, D.G., Gunsalus, K.C., Reinke, V., Kim, S.K., and Kemphues, K.J. (2002). Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans. Curr. Biol. 12, 1959-1964.
37. Praitis, V., Casey, E., Collar, D., and Austin, J. (2001). Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157, 1217-1226.
38. Reboul, J., et al. (2001). Open-reading-frame sequence tags (OSTs) support the existence of at least 17, 300 genes in C. elegans. Nat. Genet. 27, 332-336.
39. Rout, M.P., Aitchison, J.D., Suprapto, A., Hjertaas, K., Zhao, Y., and Chait, B.T. (2000). The yeast nuclear pore complex: composition, architecture, and transport mechanism. J. Cell Biol. 148, 635-651.
40. Schmiesing, J.A., Gregson, H.C., Zhou, S., and Yokomori, K. (2000). A human condensin complex containing hCAP-C-hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation. Mol. Cell. Biol. 20, 6996-7006.
41. Shulga, N., Mosammaparast, N., Wozniak, R., and Goldfarb, D.S. (2000). Yeast nucleoporins involved in passive nuclear envelope permeability. J. Cell Biol. 149, 1027-1038.
42. Siniossoglou, S., Lutzmann, M., Santos-Rosa, H., Leonard, K., Mueller, S., Aebi, U., and Hurt, E. (2000). Structure and assembly of the Nup84p complex. J. Cell Biol. 149, 41-54.
43. Siniossoglou, S., Wimmer, C., Rieger, M., Doye, V., Tekotte, H., Weise, C., Emig, S., Segref, A., and Hurt, E.C. (1996). A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell 84, 265-275.
44. Stoffler, D., Fahrenkrog, B., and Aebi, U. (1999). The nuclear pore complex: from molecular architecture to functional dynamics. Curr. Opin. Cell Biol. 11, 391-401.
45. Strome, S., Powers, J., Dunn, M., Reese, K., Malone, C.J., White, J., Seydoux, G., and Saxton, W. (2001). Spindle dynamics and the role of gamma-tubulin in early Caenorhabditis elegans embryos. Mol. Biol. Cell 12, 1751-1764.
46. Timmons, L., and Fire, A. (1998). Specific interference by ingested dsRNA. Nature 395, 854.
47. Vasu, S.K., and Forbes, D.J. (2001). Nuclear pores and nuclear assembly. Curr. Opin. Cell Biol. 13, 363-375.
48. Walther, T.C., Fornerod, M., Pickersgill, H., Goldberg, M., Allen, T.D., and Mattaj, I.W. (2001). The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins. EMBO J. 20, 5703-5714.
49. Walther, T.C., Pickersgill, H.S., Cordes, V.C., Goldberg, M.W., Allen, T.D., Mattaj, I.W., and Fornerod, M. (2002). The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import. J. Cell Biol. 158, 63-77.
50. Walther, T.C., et al. (2003). The conserved Nup107-160 complex is critical for nuclear pore complex assembly. Cell 113, 195-206.
51. Zipperlen, P., Fraser, A.G., Kamath, R.S., Martinez-Campos, M., and Ahringer, J. (2001). Roles for 147 embryonic lethal genes on C. elegans chromosome I identified by RNA interference and video microscopy. EMBO J. 20, 3984-3992.