Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p

Journal of Cell Biology

Volumn 133, Issue 6, 1996, Pages 1141-1152

  Zabel, Ulrike ^a   Doye, Valérie ^b   Tekotte, Hildegard ^c   Wepf, Roger ^c   Grandi, Paola ^d   Hurt, Eduard C ^d  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b CNRS   (France)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^d UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEOPORIN;

AMINO ACID SEQUENCE; ARTICLE; CELL NUCLEUS MEMBRANE; CHANNEL GATING; INTRACELLULAR TRANSPORT; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN PROTEIN INTERACTION; SEQUENCE ANALYSIS; YEAST;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; FUNGAL PROTEINS; GENES, FUNGAL; GENES, LETHAL; GENETIC COMPLEMENTATION TEST; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTATION; NUCLEAR ENVELOPE; NUCLEAR PORE COMPLEX PROTEINS; NUCLEAR PROTEINS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; TEMPERATURE; YEASTS;

EID: 0029900425     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.133.6.1141     Document Type: Article

References (61)

1. Adam, E.J.H., and S.A. Adam. 1994. Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope. J. Cell Biol. 125:547-555.
2. Bogerd, A.M., J.A. Hoffmann, D.C. Amberg, G.R. Fink, and L.I. Davis. 1994. nup1 mutants exhibit pleiotropic defects in nuclear pore complex function. J. Cell Biol. 127:319-332.
3. Burke, B., and L. Gerace. 1986. A cell free system to study reassembly of the nuclear envelope at the end of mitosis. Cell. 44:639-652.
4. Buss, F., and M. Stewart. 1995. Macromolecular interactions in the nucleoporin p62 complex of rat nuclear pores: binding of nucleoporin p54 to the rod domain of p62. J. Cell Biol. 128:251-261.
5. Buss, F., H. Kent, M. Stewart, S.M. Bailer, and J.A. Hanover. 1994. Role of different domains in the self-association of rat nucleoporin p62. J. Cell Sci. 107:631-638.
6. Dabauvalle, M.-C., and U. Scheer. 1991. Assembly of nuclear pore complexes in Xenopus egg extract. Biol. Cell. (Ivry Sur Seine). 72:25-29.
7. Dabauvalle, M.-C., K. Loos, and U. Scheer. 1990. Identification of a soluble precursor complex essential for nuclear pore assembly. Chromosoma (Berl). 100:56-66.
8. Doye, V., and E.C. Hurt. 1995. Genetic approaches to nuclear pore structure and function. Trends Genet. 11:193-199.
9. Doye, V., R. Wepf, and E.C. Hurt. 1994. A novel nuclear pore protein Nup133p with distinct roles in poly (A)' RNA transport and nuclear pore distribution. EMBO (Eur. Mol. Biol. Organ.) J. 13:6062-6075.
10. Elledge, S.J., and R.W. Davis. 1988. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene (Amst.). 70:303-312.
11. Enenkel, C., G. Blobel, and M. Rexach. 1995. Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes. J. Biol. Chem. 270:16499-16502.
12. Fabre, E., W.C. Boelens, C. Wimmer, I.W. Mattaj, and E.C. Hurt. 1994. Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif. Cell. 78:275-289.
13. Finlay, D.R., and D.J. Forbes. 1990. Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored. Cell. 60:17-29.
14. Finlay, D.R., E. Meier, P. Bradley, J. Horecka, and D.J. Forbes. 1991. A complex of nuclear pore proteins required for pore function. J. Cell Biol. 114:169-183.
15. Gorsch, L.C., T.C. Dockendorff, and C.N. Cole. 1995. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J. Cell Biol. 129:939-955.
16. Grandi, P., V. Doye, and E.C. Hurt. 1993. Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO (Eur. Mol. Biol. Organ.) J. 12:3061-3071.
17. Grandi, P., S. Emig, C. Weise, F. Hucho, T. Pohl, and E.C. Hurt. 1995a. A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. J. Cell Biol. 130:1263-1273.
18. Grandi, P., N. Schlaich, H. Tekotte, and E.C. Hurt. 1995b. Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p. Nup49p and a novel protein Nup57p. EMBO (Eur. Mol. Biol. Organ.) J. 14:76-87.
19. Greber, U.F., A. Senior, and L. Gerace. 1990. A major glycoprotein of the nuclear pore complex is a membrane-spanning polypeptide with a large luminal domain and a small cytoplasmic tail. EMBO (Eur. Mol. Biol. Organ.) J. 9:1495-1502.
20. Görlich, D., and E. Hartmann. 1995. A Ran-binding motif in nuclear pore proteins. Trends Cell Biol. 5:192-193.
21. Görlich, D., S. Prehn, R.A. Laskey, and E. Hartmann. 1994. Isolation of a protein that is essential for the first step of nuclear protein import. Cell. 79:767-778.
22. Grote, M., U. Kubitscheck, R. Reichelt, and R. Peters. 1995. Mapping of nucleoporins to the center of the nuclear pore complex by post-embedding immunogold electron microscopy. J Cell Sci. 108:2963-2972.
23. Hallberg, E., R.W. Wozniak, and G. Blobel. 1993. An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region. J. Cell Biol. 122:513-521.
24. Hurt, E.C. 1990. Targeting of a cytosolic protein to the nuclear periphery. J. Cell Biol. 111:2829-2837.
25. Izaurralde, E., J. Lewis, C. McGuigan, M. Jankowska, E. Darzynkiewicz, and I.W. Mattaj. 1994. A nuclear cap binding protein complex involved in pre-mRNA splicing. Cell. 78:657-668.
26. Kita, K., S. Omata, and T. Horigome. 1993. Purification and characterization of a nuclear pore glycoprotein complex containing p62. J. Biochem. (Tokyo). 113:377-382.
27. + RNA and constitutive clustering of nuclear pore complexes. Mol. Biol. Cell. 6:401-417.
28. Macaulay, C., E. Meier, and D.J. Forbes. 1995. Differential mitotic phosphorylation of proteins of the nuclear pore complex. J. Biol. Chem. 270:254-262.
29. Melchior, F., B. Paschal, J. Evans, and L. Gerace. 1993. Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J. Cell Biol. 123:1649-1659.
30. Miller, M.W., and J.A. Hanover. 1994. Functional nuclear pores reconstituted with β1-4 galactose-modified O-linked N-acetylglucosamine glycoproteins. J. Biol. Chem. 269:9289-9297.
31. Moore, M.S., and G. Blobel. 1993. The GTP-binding protein Ran/TC4 is required lor protein import into the nucleus. Nature (Lond.). 365:661-663.
32. Moore, M.S., and G. Blobel. 1994. Purification of a Ran-interacting protein that is required for protein import into the nucleus. Proc. Natl. Acad. Sci. USA. 91:10212-10216.
33. Moroianu, J., G. Blobel, and A. Radu. 1995. Previously identified protein of uncertain function is karyopherin α and together with karyopherin β docks import substrate at nuclear pore complexes. Proc. Natl. Acad. Sci. USA. 92:2008-2011.
34. Mutvei, A., S. Dihlmann, W. Herth, and E.G. Hurt. 1992. NSP1 depletion in yeast affects nuclear pore formation and nuclear accumulation. Eur. J. Cell Biol. 59:280-295.
35. Nehrbass, U., H. Kern, A. Mutvei, H. Horstmann, B. Marshallsay, and E.C. Hurt. 1990. NSP1: a yeast nuclear envelope protein localized al the nuclear pores exerts its essential function by its carboxy-terminal domain. Cell. 61:979-989.
36. Nehrbass, U., E. Fabre, S. Dihlmann, W. Herth, and E.C. Hurt. 1993. Analysis of nucleo-cytoplasmic transport in a thermosensitive mutant of the nuclear pore protein NSP1. Eur. J Cell Biol. 62:1-12.
37. Newport, J. 1987. Nuclear reconstitution in vitro: stages of assembly around protein-free DNA. Cell. 48:205-217.
38. Panté, N., and U. Aebi. 1994. Towards understanding the three-dimensional structure of the nuclear pore complex at the molecular level. Curr. Opin. Struct. Biol. 4:187-196.
39. Panté, N., R. Bastos, I. McMorrow, B. Burke, and U. Aebi. 1994. Interactions and three-dimensional localization of a group of nuclear pore complex proteins. J. Cell Biol. 126:603-617.
40. Paschal, B.M., and L. Gerace. 1995. Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J. Cell Biol. 129:925-937.
41. Pemberton, L.F., M.P. Rout, and G. Blobel. 1995. Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes. Proc. Natl. Acad. Sci. USA. 92:1187-1191.
42. Powers, M.A., and D.J. Forbes. 1994. Cytosolic factors in nuclear transport: what's importin? Cell. 79:931-934.
43. Powers, M.A., C. Macaulay, F.R. Masiarz, and D.J. Forbes. 1995. Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication. J. Cell Biol. 128:721-736.
44. Radu, A., M.S. Moore, and G. Blobel. 1995. The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex. Cell. 81:215-222.
45. Rout, M.P., and G. Blobel. 1993. Isolation of the yeast nuclear pore complex. J. Cell Biol. 123:771-783.
46. Rout, M.P., and S.R. Wente. 1994. Pore for thought: nuclear pore complex proteins. TIBS (Trends Biochem. Sci.). 4:357-363.
47. Sanger, F., S. Nicklen, and A.R. Coulson. 1977. DNA-sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:5466-5467.
48. Schlaich, N.L., and E.C. Hurt. 1995. Analysis of nucleocytoplasmic transport and nuclear envelope structure in yeast disrupted for the gene encoding the nuclear pore protein Nup1p. Eur. J. Cell Biol. 67:8-14.
49. + RNA in the cytoplasm. Proc. Natl. Acad. Sci. USA. 92:225-229.
50. Sikorski, R.S., and R. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomycex cerevisiae. Genetics. 122:19-27.
51. Simos, G., and E.C. Hurt. 1995. Nucleocytoplasmic transport: factors and mechanisms. FEBS Lett. 369:107-112.
52. Simos, G., H. Tekotte, H. Grosjean, A. Segref, K. Sharma, D. Tollervey, and E.C. Hurt. 1996. Nuclear pore proteins are involved in the biogenesis of functional tRNA. EMBO (Eur. Mol. Biol. Organ.) J. 15:2270-2284.
53. Siniossoglou, S., C. Wimmer, M. Rieger, V. Doye, H. Tekotte, C. Weise, S. Emig, A. Segref, and E.C. Hurt. 1996. A novel complex of nucleoporins which includes Sec13p and a Sec13p homologue is essential for normal nuclear pores. Cell. 84:265-275.
54. Sukegawa, J., and G. Blobel. 1993. A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm. Cell. 72:29-38.
55. Weis, K., I.W. Mattaj, and A.I. Lamond. 1995. Identification of hSRP1a as a functional receptor for nuclear localization sequences. Science (Wash. DC). 268:1049-1053.
56. Wente, S.R., and G. Blobel. 1993. A temperature-sensitive NUPI16 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic. J. Cell Biol. 123:275-284.
57. Wente, S.R., and G. Blobel. 1994. NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure. J. Cell Biol. 125:955-969.
58. Wilken, N., U. Kossner, J.-L. Senécal, U. Scheer, and M.-C. Dabauvalle. 1993. Nup180, a novel nuclear pore complex protein localizing to the cytoplasmic ring and associated fibrils. J. Cell Biol. 123:1345-1354.
59. Wimmer, C., V. Doye, P. Grandi, U. Nehrbass, and E. Hurt. 1992. A new subclass of nucleoporins that functionally interacts with nuclear pore protein NSP1. EMBO (Eur. Mol. Biol. Organ.) J. 11:5051-5061.
60. Wozniak, R.W., G. Blobel, and M.P. Rout. 1994. POM152 is an integral protein of the pore membrane domain of the yeast nuclear envelope. J. Cell Biol. 125:31-42.
61. Yokoyama, N., N. Hayashi, T. Seki, N. Panté, T. Ohba, K. Nishii, K. Kuma, T. Hayashida, T. Miyata, U. Aebi et al. 1995. A giant nucleopore protein that binds Ran/TC4. Nature (Lond.). 376:184-188.