Mapping the dynamic organization of the nuclear pore complex inside single living cells

Nature Cell Biology

Volumn 6, Issue 11, 2004, Pages 1114-1121

  Rabut, Gwénaël ^a   Doye, Valérie ^b   Ellenberg, Jan ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^b INSTITUT CURIE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

POLYPEPTIDE; PROTEIN SUBUNIT;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL STRUCTURE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DISSOCIATION; GENE MAPPING; NONHUMAN; NUCLEAR PORE COMPLEX; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION; RAT; REGULATORY MECHANISM;

CELLS, CULTURED; KINETICS; NUCLEAR PORE; PLASMIDS; PROTEOME;

ANIMALIA; VERTEBRATA;

EID: 7944236264     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/ncb1184     Document Type: Article

References (31)

1. Hartwell, L. H., Hopfield, J. J., Leibler, S. & Murray, A. W. From molecular to modular cell biology. Nature 402, C47-C52 (1999).
2. Cronshaw, J. M., Krutchinsky, A. N., Zhang, W., Chait, B. T. & Matunis, M. J. Proteomic analysis of the mammalian nuclear pore complex. J. Cell Biol. 158, 915-927 (2002).
3. Suntharalingam, M. & Wente, S. R. Peering through the pore: nuclear pore complex structure, assembly, and function. Dev. Cell 4, 775-789 (2003).
4. Daigle, N. et al. Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells. J. Cell Biol. 154, 71-84 (2001).
5. Enninga, J., Levay, A. & Fontoura, B. M. Sec13 shuttles between the nucleus and the cytoplasm and stably interacts with Nup96 at the nuclear pore complex. Mol. Cell. Biol. 23, 7271-7284 (2003).
6. Belgareh, N. et al. An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells. J. Cell Biol. 154, 1147-1160 (2001).
7. Nakielny, S., Shaikh, S., Burke, B. & Dreyfuss, G. Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain. EMBO J. 18, 1982-1995 (1999).
8. Griffis, E. R., Altan, N., Lippincott-Schwartz, J. & Powers, M. A. Nup98 is a mobile nucleoporin with transcription-dependent dynamics. Mol. Biol. Cell 13, 1282-1297 (2002).
9. Zolotukhin, A. S. & Felber, B. K. Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev. J. Virol. 73, 120-127 (1999).
10. Dilworth, D. J. et al. Nup2p dynamically associates with the distal regions of the yeast nuclear pore complex. J. Cell Biol. 153, 1465-1478 (2001).
11. Lindsay, M. E., Plafker, K., Smith, A. E., Clurman, B. E. & Macara, I. G. Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import. Cell 110, 349-360 (2002).
12. Denning, D. et al. The nucleoporin Nup60p functions as a Gsp1p-GTP-sensitive tether for Nup2p at the nuclear pore complex. J. Cell Biol. 154, 937-950 (2001).
13. Guan, T. et al. Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein export. Mol. Cell. Biol. 20, 5619-5630 (2000).
14. Bodoor, K. et al. Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis. J. Cell Sci. 112, 2253-2264 (1999).
15. Dundr, M., McNally, J. G., Cohen, J. & Misteli, T. Quantitation of GFP-fusion proteins in single living cells. J. Struct. Biol. 140, 92-99 (2002).
16. Dundr, M. et al. A kinetic framework for a mammalian RNA polymerase in vivo. Science 298, 1623-1626 (2002).
17. Loiodice, I. et al. The entire nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis. Mol. Biol. Cell 15, 3333-3344 (2004).
18. Harel, A. et al. Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores. Mol. Cell 11, 853-864 (2003).
19. Walther, T. C. et al. The conserved Nup107-160 complex is critical for nuclear pore complex assembly. Cell 113, 195-206 (2003).
20. Walther, T. C. et al. The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import, J. Cell Biol. 158, 63-77 (2002).
21. Cronshaw, J. M. & Matunis, M. J. The nuclear pore complex protein ALADIN is mislocalized in triple A syndrome. Proc. Natl Acad. Sci. USA 100, 5823-5827 (2003).
22. Drummond, S. P. & Wilson, K. L. Interference with the cytoplasmic tail of gp210 disrupts "close apposition" of nuclear membranes and blocks nuclear pore dilation. J. Cell Biol. 158, 53-62 (2002).
23. Olsson, M., Scheele, S. & Ekblom, P. Limited expression of nuclear pore membrane glycoprotein 210 in cell lines and tissues suggests cell-type specific nuclear pores in metazoans. Exp. Cell Res. 292, 359-370 (2004).
24. Galy, V., Mattaj, I. W. & Askjaer, P. Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo. Mol. Biol. Cell 14, 5104-5115 (2003).
25. Griffis, E. R., Craige, B., Dimaano, C., Ullman, K. S. & Powers, M. A. Distinct functional domains within nucleoporins Nup153 and Nup98 mediate transcription-dependent mobility. Mol. Biol. Cell 15, 1991-2002 (2004).
26. Macara, I. G. Transport into and out of the nucleus. Microbiol. Mol. Biol. Rev. 65, 570-594 (2001).
27. Smith, A. E., Slepchenko, B. M., Schaff, J. C., Loew, L. M. & Macara, I. G. Systems analysis of Ran transport. Science 295, 488-491 (2002).
28. Ribbeck, K. & Gorlich, D. Kinetic analysis of translocation through nuclear pore complexes. EMBO J. 20, 1320-1330 (2001).
29. Carrero, G., McDonald, D., Crawford, E., de Vries, G. & Hendzel, M. J. Using FRAP and mathematical modeling to determine the in vivo kinetics of nuclear proteins. Methods 29, 14-28 (2003).
30. Janin, J. in Protein-Protein Recognition (ed. Kleanthous, C.) 1-32 (Oxford University Press, New York, 2000).
31. Rabut, G. & Ellenberg, J. J. Microsc. Automatic real-time 3D cell tracking by fluorescence microscopy. 216, 131-137 (2004).