Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores

Molecular Cell

Volumn 11, Issue 4, 2003, Pages 853-864

  Harel, Amnon ^a   Orjalo, Arturo V ^a   Vincent, Thomas ^a,c   Lachish Zalait, Aurelie ^b   Vasu, Sanjay ^a,d   Shah, Sundeep ^a,e   Zimmerman, Ella ^b   Elbaum, Michael ^b   Forbes, Douglass J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^c ISIS PHARMACEUTICALS   (United States)

^d Quorex Pharmaceuticals   (United States)

^e Aurora Biosciences Corporation   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN; GLYCOPROTEIN 210; INTERGRAL MEMBRANE PROTEIN POM121; NUCLEOPORIN; PROTEIN; PROTEIN ANTIBODY; PROTEIN NUP107 160; PROTEIN NUP133; PROTEIN NUP85; PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

ARTICLE; CELL NUCLEUS; DNA REPLICATION; NONHUMAN; NUCLEAR PORE; PROTEIN ANALYSIS; PROTEIN DEFECT; SCANNING ELECTRON MICROSCOPY; VERTEBRATE;

VERTEBRATA;

EID: 0037547118     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(03)00116-3     Document Type: Article

References (66)

1. Aitchison J.D., Blobel G., Rout M.P. Nup120p. A yeast nucleoporin required for nuclear pore distribution and mRNA transport J. Cell Biol. 131:1995;1659-1675.
2. Allen T.D., Rutherford S.Z., Bennion G.R., Wiese C., Riepert S., Kiseleva E., Goldberg M.W. Three-dimensional surface structure analysis of the nucleus. Berrios M. Methods in Cell Biology, Vol. 53. 1998;Academic Press, San Diego. 125-138.pp.
3. Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J. Gapped BLAST and PSI-BLAST. a new generation of protein database search programs Nucleic Acids Res. 25:1997;3389-3402.
4. Bastos R., Lin A., Enarson M., Burke B. Targeting and function in mRNA export of nuclear pore complex protein Nup153. J. Cell Biol. 134:1996;1141-1156.
5. Belgareh N., Rabut G., Bai S.W., van Overbeek M., Beaudouin J., Daigle N., Zatsepina O.V., Pasteau F., Labas V., Fromont-Racine M.et al. An evolutionarily conserved nuclear pore subcomplex, which redistributes in part to kinetochores in mammalian cells. J. Cell Biol. 152:2001;1147-1160.
6. Blow J.J., Laskey R.A. Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs. Cell. 47:1986;577-587.
7. Bodoor K., Shaikh S., Salina D., Raharjo W.H., Bastos R., Lohka M., Burke B. Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis. J. Cell Sci. 112:1999;2253-2264.
8. Boehmer T., Enninga J., Dales S., Blobel G., Zhong H. Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex. Proc. Natl. Acad. Sci. USA. 100:2003;981-985.
9. Conti E., Izaurralde E. Nucleocytoplasmic transport enters the atomic age. Curr. Opin. Cell Biol. 13:2001;310-319.
10. Cronshaw J.M., Krutchinsky A.N., Zhang W., Chait B.T., Matunis M.J. Proteomic analysis of the mammalian nuclear pore complex. J. Cell Biol. 158:2002;915-927.
11. Cullen B.R. Journey to the center of the cell. Cell. 105:2001;697-700.
12. Damelin M., Silver P.A. Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex. Mol. Cell. 5:2000;133-140.
13. Dimaano C., Bal J.R., Prunuske A.J., Ullman K.S. RNA association defines a functionally conserved domain in the nuclear protein Nup153. J. Biol. Chem. 276:2001;45349-45357.
14. Doye V., Wepf R., Hurt E.C. A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution. EMBO J. 13:1994;6062-6075.
15. Drummond S.P., Wilson K.L. Interference with the cytoplasmic tail of gp210 disrupts "close apposition" of nuclear membranes and blocks nuclear pore dilation. J. Cell Biol. 158:2002;53-62.
16. Drummond S., Ferrigno P., Lyon C., Murphy J., Goldberg M., Allen T., Smythe C., Hutchison C.J. Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types. J. Cell Biol. 144:1999;225-240.
17. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:2001;494-498.
18. Feldherr C.M., Akin D., Cohen R.J. Regulation of functional nuclear pore size in fibroblasts. J. Cell Sci. 114:2001;4621-4627.
19. Finlay D.R., Meier E., Bradley P., Horecka J., Forbes D.J. A complex of nuclear pore proteins required for pore function. J. Cell Biol. 114:1991;169-183.
20. Fontoura B.M., Blobel G., Matunis M.J. A conserved biogenesis pathway for nucleoporins. proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96 J. Cell Biol. 144:1999;1097-1112.
21. Forbes D.J., Kirschner M.W., Newport J.W. Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs. Cell. 34:1983;13-23.
22. Goldberg M.W., Wiese C., Allen T.D., Wilson K.L. Dimples, pores, star-rings, and thin rings on growing nuclear envelopes. evidence for structural intermediates in nuclear pore complex assembly J. Cell Sci. 110:1997;409-420.
23. Goldstein A.L., Snay C.A., Heath C.V., Cole C.N. Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p. Mol. Biol. Cell. 7:1996;917-934.
24. Gorlich D., Kutay U. Transport between the cell nucleus and the cytoplasm. Annu. Rev. Cell Dev. Biol. 15:1999;607-660.
25. Grandi P., Dang T., Pane N., Shevchenko A., Mann M., Forbes D., Hurt E. Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly. Mol. Biol. Cell. 8:1997;2017-2038.
26. Greber U.F., Senior A., Gerace L. A major glycoprotein of the nuclear pore complex is a membrane-spanning polypeptide with a large lumenal domain and a small cytoplasmic tail. EMBO J. 9:1990;1495-1502.
27. Griffis E.R., Altan N., Lippincott-Schwartz J., Powers M.A. Nup98 is a mobile nucleoporin with transcription-dependent dynamics. Mol. Biol. Cell. 13:2002;1282-1297.
28. Hallberg E., Wozniak R.W., Blobel G. An integral membrane protein of the pore membrane domain of the nuclear envelope contains a nucleoporin-like region. J. Cell Biol. 122:1993;513-521.
29. Hetzer M., Meyer H.H., Walther T.C., Bilbao-Cortes D., Warren G., Mattaj I.W. Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly. Nat. Cell Biol. 3:2001;1086-1091.
30. Hinshaw J.E., Carragher B.O., Milligan R.A. Architecture and design of the nuclear pore complex. Cell. 69:1992;1133-1141.
31. Kalab P., Weis K., Heald R. Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts. Science. 295:2002;2452-2456.
32. Li O., Heath C.V., Amberg D.C., Dockendorff T.C., Copeland C.S., Snyder M., Cole C.N. Mutation or deletion of the S. cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A+) RNA and constitutive clustering of nuclear pore complexes. Mol. Biol. Cell. 6:1995;401-417.
33. Lohka M.J., Masui Y. Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components. Science. 220:1983;719-721.
34. Lutzmann M., Kunze R., Buerer A., Aebi U., Hurt E. Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins. EMBO J. 21:2002;387-397.
35. Lyman S.K., Gerace L. Nuclear pore complexes. dynamics in unexpected places J. Cell Biol. 154:2001;17-20.
36. Macara I.G. Why FRET about Ran? Dev. Cell. 2:2002;379-380.
37. Macaulay C., Forbes D.J. Assembly of the nuclear pore. biochemically distinct steps revealed with NEM, GTPγS, and BAPTA J. Cell Biol. 132:1996;5-20.
38. Marelli M., Lusk C.P., Chan H., Aitchison J.D., Wozniak R.W. A link between the synthesis of nucleoporins and the biogenesis of the nuclear envelope. J. Cell Biol. 153:2001;709-724.
39. Matsuoka Y., Takagi M., Ban T., Miyazaki M., Yamamoto T., Kondo Y., Yoneda Y. Identification and characterization of nuclear pore subcomplexes in mitotic extract of human somatic cells. Biochem. Biophys. Res. Commun. 254:1999;417-423.
40. Miller B.R., Forbes D.J. Purification of the vertebrate nuclear pore by biochemical criteria. Traffic. 1:2000;941-951.
41. Newport J. Nuclear reconstitution in vitro. stages of assembly around protein-free DNA Cell. 48:1987;205-217.
42. Powers M.A., Macaulay C., Masiarz F.R., Forbes D.J. Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97 (Nup98), import but are defective in nuclear growth and replication. J. Cell Biol. 128:1995;721-736.
43. Powers M.A., Forbes D.J., Dahlberg J.E., Lund E. The vertebrate GLFG nucleoporin, Nup98, is an essential component of multiple RNA export pathways. J. Cell Biol. 136:1997;241-250.
44. Reichelt R., Holzenburg A., Buhle E.L. Jr., Jarnik M., Engel A., Aebi U. Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components. J. Cell Biol. 110:1990;883-894.
45. Ribbeck K., Gorlich D. The permeability barrier of nuclear pore complexes appears to operate via hydrophobic exclusion. EMBO J. 21:2002;2664-2671.
46. Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T. The yeast nuclear pore complex. Composition, architecture, and transport mechanism. J. Cell Biol. 148:2000;635-652.
47. Ryan K.J., Wente S.R. Isolation and characterization of new Saccharomyces cerevisiae mutants perturbed in nuclear pore complex assembly. BMC Genet. 3:2002;17.
48. Sazer S., Dasso M. The ran decathlon. multiple roles of Ran J. Cell Sci. 113:2000;1111-1118.
49. Schwoebel E.D., Ho T.H., Moore M.S. The mechanism of inhibition of Ran-dependent nuclear transport by cellular ATP depletion. J. Cell Biol. 157:2002;963-974.
50. Shah S., Tugendreich S., Forbes D. Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr. J. Cell Biol. 141:1998;31-49.
51. Shamsher M.K., Ploski J., Radu A. Karyopherin beta 2B participates in mRNA export from the nucleus. Proc. Natl. Acad. Sci. USA. 99:2002;14195-14199.
52. Shaywitz D.A., Orci L., Ravazzola M., Swaroop A., Kaiser C.A. Human SEC13Rp functions in yeast and is located on transport vesicles budding from the endoplasmic reticulum. J. Cell Biol. 128:1995;769-777.
53. Siebrasse J.P., Peters R. Rapid translocation of NTF2 through the nuclear pore of isolated nuclei and nuclear envelopes. EMBO Rep. 3:2002;887-892.
54. Siniossoglou S., Wimmer C., Rieger M., Doye V., Tekotte H., Weise C., Emig S., Segref A., Hurt E.C. A novel complex of nucleoporins, which includes sec13p and a sec13p homologue, is essential for normal nuclear pores. Cell. 84:1996;265-275.
55. Siniossoglou S., Lutzmann M., Santos-Rosa H., Leonard K., Mueller S., Aebi U., Hurt E. Structure and assembly of the Nup84p complex. J. Cell Biol. 149:2000;41-54.
56. Ullman K.S., Forbes D.J. RNA polymerase III transcription in synthetic nuclei assembled in vitro from defined DNA templates. Mol. Cell. Biol. 15:1995;4873-4883.
57. Ullman K., Shah S., Powers M., Forbes D. The nucleoporin Nup153 plays a critical role in multiple types of nuclear export. Mol. Biol. Cell. 10:1999;649-664.
58. Vasu S., Forbes D.J. Nuclear pores and nuclear assembly. Curr. Opin. Cell Biol. 13:2001;363-375.
59. Vasu S., Shah S., Orjalo A., Park M., Fischer W.H., Forbes D.J. Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export. J. Cell Biol. 155:2001;339-354.
60. Vigers G.P., Lohka M.J. A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs. J. Cell Biol. 112:1991;545-556.
61. Walther T.C., Fornerod M., Pickersgill H., Goldberg M., Allen T.D., Mattaj I.W. The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins. EMBO J. 20:2001;5703-5714.
62. Walther T.C., Pickersgill H.S., Cordes V.C., Goldberg M.W., Allen T.D., Mattaj I.W., Fornerod M. The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import. J. Cell Biol. 158:2002;63-77.
63. Weis K. Nucleocytoplasmic transport. cargo trafficking across the border Curr. Opin. Cell Biol. 14:2002;328-335.
64. Wiese C., Goldberg M.W., Allen T.D., Wilson K.L. Nuclear envelope assembly in Xenopus extracts visualized by scanning EM reveals a transport-dependent 'envelope smoothing' event. J. Cell Sci. 110:1997;1489-1502.
65. Yang Q., Rout M.P., Akey C.W. Three-dimensional architecture of the isolated yeast nuclear pore complex. functional and evolutionary implications Mol. Cell. 1:1998;223-234.
66. Zhang C., Clarke P.R. Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts. Science. 288:2000;1429-1432.