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The shrinking of the PEO chain was further proved by using a more hydrophobic spreading solvent (e.g., hexane). The isotherm (Figure S1 c in the Supporting Information) was slightly removed to smaller molecular area compared with using chloroform spreading solvent. This indicates that the PEO chain was shrunk to some extent. The AFM images of the film deposited using hexane solvent do not show much difference, only the aggregate formed at relatively low surface pressure compared with using cyclohexane solvent. This further confirmed the critical role of the hydrophobic segments in the formation of toroid structures.
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