Synergistic toxicity of ethanol and MDMA towards primary cultured rat hepatocytes

Toxicology

Volumn 254, Issue 1-2, 2008, Pages 42-50

  Pontes, Helena ^a   Sousa, Carla ^a   Silva, Renata ^a   Fernandes, Eduarda ^a   Carmo, Helena ^a   Remião, Fernando ^a   Carvalho, Félix ^a   Bastos, Maria Lourdes ^a  

^a Campus Universitário de Santiago   (Portugal)

Author keywords

3,4 Methylenedioxymethamphetamine; Ethanol; Hepatotoxicity; Hyperthermia; Oxidative stress; Primary cultured rat hepatocytes

Indexed keywords

3,4 METHYLENEDIOXYMETHAMPHETAMINE; ACRIDINE ORANGE; ADENOSINE TRIPHOSPHATE; ALCOHOL; APOPTOSIS INDUCING FACTOR; CASPASE 3; CYTOCHROME C; ETHIDIUM BROMIDE; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE; GLUTATHIONE DISULFIDE; GLUTATHIONE TRANSFERASE; LACTATE DEHYDROGENASE; PROTEIN BAX; REACTIVE NITROGEN SPECIES; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CELL DEATH; CELL ENERGY; CONTROLLED STUDY; DRUG ALCOHOL INTERACTION; ENZYME ACTIVATION; ENZYME ACTIVITY; HIGH TEMPERATURE; LIVER CELL; LIVER MITOCHONDRION; LIVER NECROSIS; LIVER TOXICITY; MALE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN SECRETION; PROTEIN TRANSPORT; RAT; WESTERN BLOTTING;

ADENOSINE TRIPHOSPHATE; ANIMALS; CELL SURVIVAL; CELLS, CULTURED; DRUG SYNERGISM; ETHANOL; HEPATOCYTES; HOT TEMPERATURE; MALE; N-METHYL-3,4-METHYLENEDIOXYAMPHETAMINE; OXIDATIVE STRESS; RATS; RATS, WISTAR;

RATTUS;

EID: 56049126380     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tox.2008.09.009     Document Type: Article

References (72)

1. Alvaro A.R., Martins J., Costa A.C., Fernandes E., Carvalho F., Ambrósio A.F., and Cavadas C. Neuropeptide Y protects retinal neural cells against cell death induced by ecstasy. Neuroscience 152 (2008) 97-105
2. Anderson M.E. Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol. 113 (1985) 548-555
3. Barrett S.P., Darredeau C., and Pihl R.O. Patterns of simultaneous polysubstance use in drug using university students. Hum. Psychopharmacol. 21 (2006) 255-263
4. Beitia G., Cobreros A., Sainz L., and Cenarruzabeitia E. 3,4-Methylenedioxymethamphetamine (ecstasy)-induced hepatotoxicity: effect on cytosolic calcium signals in isolated hepatocytes. Liver 19 (1999) 234-241
5. Bergermeyer H.U., and Bernt E. UV-assay for lactate dehydrogenase with pyruvate and NADH. In: Bergermeyer H.U. (Ed). Methods of Enzymatic Analysis (1974), Verlag Chemie GmbH, Weinheim
6. Bindoli A., Rigobello M.P., and Deeble D.J. Biochemical and toxicological properties of the oxidation products of catecholamines. Free Radic. Biol. Med. 13 (1992) 391-405
7. Boujrad H., Gubkina O., Robert N., Krantic S., and Susin S.A. AIF-mediated programmed necrosis: a highly regulated way to die. Cell Cycle 6 (2007) 2612-2619
8. Boyd C.J., McCabe S.E., and d'Arcy H. Ecstasy use among college undergraduates: gender, race and sexual identity. J. Subst. Abuse Treat. 24 (2003) 209-215
9. Breen C., Degenhardt L., Kinner S., Bruno R., Jenkinson R., Matthews A., and Newman J. Alcohol use and risk taking among regular ecstasy users. Subst. Use Misuse 41 (2006) 1095-1109
10. Capela J.P., Macedo C., Branco P.S., Ferreira L.M., Lobo A.M., Fernandes E., Remião F., Bastos M.L., Dirnagl U., Meisel A., and Carvalho F. Neurotoxicity mechanisms of thioether ecstasy metabolites. Neuroscience 146 (2007) 1743-1757
11. Carmo H., Brulport M., Hermes M., Oesch F., de Boer D., Remião F., Carvalho F., Schön M.R., Krebsfaenger N., Doehmer J., Bastos M.L., and Hengstler J.G. CYP2D6 increases toxicity of the designer drug 4-methylthioamphetamine (4-MTA). Toxicology 229 (2007) 236-244
12. Carvalho M., Carvalho F., and Bastos M.L. Is hyperthermia the triggering factor for hepatotoxicity induced by 3,4-methylenedioxymethamphetamine (ecstasy)? An in vitro study using freshly isolated mouse hepatocytes. Arch. Toxicol. 74 (2001) 789-793
13. Carvalho M., Milhazes N., Remião F., Borges F., Fernandes E., Amado F., Monks T.J., Carvalho F., and Bastos M.L. Hepatotoxicity of 3,4-methylenedioxyamphetamine and alpha-methyldopamine in isolated rat hepatocytes: formation of glutathione conjugates. Arch. Toxicol. 78 (2004) 16-24
14. Carvalho M., Remião F., Milhazes N., Borges F., Fernandes E., Carvalho F., and Bastos M.L. The toxicity of N-methyl-alpha-methyldopamine to freshly isolated rat hepatocytes is prevented by ascorbic acid and N-acetylcysteine. Toxicology 200 (2004) 193-203
15. Castilla R., González R., Fouad D., Fraga E., and Muntane J. Dual effect of ethanol on cell death in primary culture of human and rat hepatocytes. Alcohol Alcohol. 39 (2004) 290-296
16. Colado M.I., O'Shea E., Granados R., Murray T.K., and Green A.R. In vivo evidence for free radical involvement in the degeneration of rat brain 5-HT following administration of MDMA ('ecstasy') and p-chloroamphetamine but not the degeneration following fenfluramine. Br. J. Pharmacol. 121 (1997) 889-900
17. Colell A., García-Ruiz C., Miranda M., Ardite E., Marí M., Morales A., Corrales F., Kaplowitz N., and Fernández-Checa J.C. Selective glutathione depletion of mitochondria by ethanol sensitizes hepatocytes to tumor necrosis factor. Gastroenterology 115 (1998) 1541-1551
18. Cunningham C.C., Coleman W.B., and Spach P.I. The effects of chronic ethanol consumption on hepatic mitochondrial energy metabolism. Alcohol Alcohol. 25 (1990) 127-136
19. Das S.K., and Vasudevan D.M. Alcohol-induced oxidative stress. Life Sci. 81 (2007) 177-187
20. EMCDDA, 2005. Annual report 2005: The state of the drugs problem in Europe.
21. Fadeel B., Ottosson A., and Pervaiz S. Big wheel keeps on turning: apoptosome regulation and its role in chemoresistance. Cell Death Differ. 15 (2008) 443-452
22. Garcia-Repetto R., Moreno E., Soriano T., Jurado C., Gimenez M.P., and Menendez M. Tissue concentrations of MDMA and its metabolite MDA in three fatal cases of overdose. Forensic Sci. Int. 135 (2003) 110-114
23. Gebhardt R., Hengstler J.G., Müller D., Glöckner R., Buenning P., Laube B., Schmelzer E., Ullrich M., Utesch D., Hewitt N., Ringel M., Hilz B.R., Bader A., Langsch A., Koose T., Burger H.J., Maas J., and Oesch F. New hepatocyte in vitro systems for drug metabolism: metabolic capacity and recommendations for application in basic research and drug development, standard operation procedures. Drug Metab. Rev. 35 (2003) 145-213
24. Gemma S., Vichi S., and Testai E. Individual susceptibility and alcohol effects: biochemical and genetic aspects. Ann. Ist. Super. Sanita 42 (2006) 8-16
25. Gordon C.J. Thermophysiological responses to hyperthermic drugs: extrapolating from rodent to human. Prog. Brain Res. 162 (2007) 63-79
26. Gouzoulis-Mayfrank E., and Daumann J. The confounding problem of polydrug use in recreational ecstasy/MDMA users: a brief overview. J. Psychopharmacol. 20 (2006) 188-193
27. Green A.R., Mechan A.O., Elliott J.M., O'Shea E., and Colado M.I. The pharmacology and clinical pharmacology of 3,4-methylenedioxymethamphetamine (MDMA, "ecstasy"). Pharmacol. Rev. 55 (2003) 508-563
28. Green A.R., O'shea E., and Colado M.I. A review of the mechanisms involved in the acute MDMA (ecstasy)-induced hyperthermic response. Eur. J. Pharmacol. 500 (2004) 3-13
29. Gyamfi M.A., and Wan Y.J. The effect of ethanol, ethanol metabolizing enzyme inhibitors, and Vitamin E on regulating glutathione, glutathione S-transferase, and S-adenosylmethionine in mouse primary hepatocyte. Hepatol. Res. 35 (2006) 53-61
30. Habig W.H., Pabst M.J., and Jakoby W.B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249 (1974) 7130-7139
31. Helzberg J.H., Brown M.S., Smith D.J., Gore J.C., and Gordon E.R. Metabolic state of the rat liver with ethanol: comparison of in vivo 31 phosphorus nuclear magnetic resonance spectroscopy with freeze clamp assessment. Hepatology 7 (1987) 83-88
32. Hernández-López C., Farré M., Roset P.N., Menoyo E., Pizerro N., Ortuño J., Torrens M., Cami J., and de la Torre R. 3,4-Methylenedioxymethamphetamine (ecstasy) and alcohol interactions in humans: psychomotor performance, subjective effects, and pharmacokinetics. J. Pharmacol. Exp. Ther. 300 (2002) 236-244
33. Hewitt N.J., Lechón M.J., Houston J.B., Hallifax D., Brown H.S., Maurel P., Kenna J.G., Gustavsson L., Lohmann C., Skonberg C., Guillouzo A., Tuschl G., Li A.P., LeCluyse E., Groothuis G.M., and Hengstler J.G. Primary hepatocytes: current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies. Drug Metab. Rev. 39 (2007) 159-234
34. Hiramatsu M., Kumagai Y., Unger S.E., and Cho A.K. Metabolism of methylenedioxymethamphetamine: formation of dihydroxymethamphetamine and a quinone identified as its glutathione adduct. J. Pharmacol. Exp. Ther. 254 (1990) 521-527
35. Hopper J.W., Su Z., Looby A.R., Ryan E.T., Penetar D.M., Palmer C.M., and Lukas S.E. Incidence and patterns of polydrug use and craving for ecstasy in regular ecstasy users: An ecological momentary assessment study. Drug Alcohol Depend. 85 (2006) 221-235
36. Institute for Laboratory Animal Research. Guide for the Care and Use of Laboratory Animals (1996), National Academy Press, Washington, D.C.
37. Jang G.R., and Harris R. Drug interactions involving ethanol and alcoholic beverages. Expert Opin. Drug Metab. Toxicol. 3 (2007) 719-731
38. Jiménez A., Jordà E.G., Verdaguer E., Pubill D., Sureda F.X., Canudas A.M., Escubedo E., Camarasa J., Camins A., and Pallàs M. Neurotoxicity of amphetamine derivatives is mediated by caspase pathway activation in rat cerebellar granule cells. Toxicol. Appl. Pharmacol. 196 (2004) 223-234
39. Johnson E.A., Shvedova A.A., Kisin E., O'Callaghan J.P., Kommineni C., and Miller D.B. d-MDMA during vitamin E deficiency: effects on dopaminergic neurotoxicity and hepatotoxicity. Brain Res. 933 (2002) 150-163
40. Lauterburg B.H., Davies S., and Mitchell J.R. Ethanol suppresses hepatic glutathione synthesis in rats in vivo. J. Pharmacol. Exp. Ther. 230 (1984) 7-11
41. Leier I., Jedlitschky G., Buchholz U., Center M., Cole S.P., Deeley R.G., and Keppler D. ATP-dependent glutathione disulphide transport mediated by the MRP gene-encoded conjugate export pump. Biochem. J. 14 (1996) 433-437
42. Liechti M.E., Kunz I., and Kupferschmidt H. Acute medical problems due to Ecstasy use. Case-series of emergency department visits. Swiss Med. Wkly. 135 (2005) 652-657
43. Lora-Tamayo C., Tena T., Rodriguez A., Moreno D., Sancho J.R., Ensenat P., and Muela F. The designer drug situation in Ibiza. Forensic Sci. Int. 140 (2004) 195-206
44. Lorenzo H.K., and Susin S.A. Therapeutic potential of AIF-mediated caspase-independent programmed cell death. Drug Resist. Updat. 10 (2007) 235-255
45. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
46. Mannervik B., and Danielson U.H. Glutathione transferases-structure and catalytic activity. CRC Crit. Rev. Biochem. 23 (1988) 283-337
47. Milroy C.M., Clark J.C., and Forrest A.R. Pathology of deaths associated with "ecstasy" and "eve" misuse. J. Clin. Pathol. 49 (1996) 149-153
48. Monks T.J., Jones D.C., Bai F., and Lau S.S. The role of metabolism in 3,4-(+)-methylenedioxyamphetamine and 3,4-(+)-methylenedioxymethamphetamine (ecstasy) toxicity. Ther. Drug Monit. 26 (2004) 132-136
49. Monks T.J., and Lau S.S. Toxicology of quinone-thioethers. Crit. Rev. Toxicol. 22 (1992) 243-270
50. Montiel-Duarte C., Ansorena E., López-Zabalza M.J., Cenarruzabeitia E., and Iraburu M.J. Role of reactive oxygen species, glutathione and NF-kappaB in apoptosis induced by 3,4-methylenedioxymethamphetamine ("Ecstasy") on hepatic stellate cells. Biochem. Pharmacol. 67 (2004) 1025-1033
51. Montiel-Duarte C., Varela-Rey M., Osés-Prieto J.A., López-Zabalza M.J., Beitia G., Cenarruzabeitia E., and Iraburu M.J. 3,4-Methylenedioxymethamphetamine ("Ecstasy") induces apoptosis of cultured rat liver cells. Biochim. Biophys. Acta 1588 (2002) 26-32
52. Morimoto M., Hagbjörk A.L., Nanji A.A., Ingelman-Sundberg M., Lindros K.O., Fu P.C., Albano E., and French S.W. Role of cytochrome P4502E1 in alcoholic liver disease pathogenesis. Alcohol 10 (1993) 459-464
53. Oyefeso A., Schifano F., Ghodse H., Cobain K., Dryden R., and Corkery J. Fatal injuries while under the influence of psychoactive drugs: a cross-sectional exploratory study in England. BMC Public Health 6 (2006) 148
54. Piner P., Sevgiler Y., and Uner N. In vivo effects of fenthion on oxidative processes by the modulation of glutathione metabolism in the brain of Oreochromis niloticus. Environ. Toxicol. 22 (2007) 605-612
55. Piquet M.A., Roulet M., Nogueira V., Filippi C., Sibille B., Hourmand-Ollivier I., Pilet M., Rouleau V., and Leverve X.M. Polyunsaturated fatty acid deficiency reverses effects of alcohol on mitochondrial energy metabolism. J. Hepatol. 41 (2004) 721-729
56. Pontes H., Duarte J.A., Guedes de Pinho P., Soares M.E., Fernandes E., Dinis-Oliveira R.J., Sousa C., Silva R., Carmo H., Casal S., Remião F., Carvalho F., and Bastos M.L. Chronic exposure to ethanol exacerbates MDMA-induced hyperthermia and exposes liver to severe MDMA-induced toxicity in CD1 mice. Toxicology 252 (2008) 64-71
57. Pontes H., Santos-Marques M.J., Fernandes E., Duarte J.A., Remião F., Carvalho F., and Bastos M.L. Effect of chronic ethanol exposure on the hepatotoxicity of ecstasy in mice: An ex vivo study. Toxicol. In Vitro 22 (2008) 910-920
58. Quinton M.S., and Yamamoto B.K. Causes and consequences of methamphetamine and MDMA toxicity. AAPS J. 8 (2006) E337-E347
59. SAMHSA, 2005. 2005 National Survey on Drug Use and Health: National Findings.
60. Sanjurjo E., Nogue S., Miro O., and Munne P. Analysis of patients attended in an emergency department due to ecstasy consumption. Med. Clin. (Barc.) 123 (2004) 90-92
61. Sastre J., Serviddio G., Pereda J., Minana J.B., Arduini A., Vendemiale G., Poli G., Pallardo F.V., and Vina J. Mitochondrial function in liver disease. Front. Biosci. 12 (2007) 1200-1209
62. Schifano F. A bitter pill. Overview of ecstasy (MDMA, MDA) related fatalities. Psychopharmacology (Berl.) 173 (2004) 242-248
63. Schifano F., Corkery J., Deluca P., Oyefeso A., and Ghodse A.H. Ecstasy (MDMA, MDA, MDEA, MBDB) consumption, seizures, related offences, prices, dosage levels and deaths in the UK (1994-2003). J. Psychopharmacol. 20 (2006) 456-463
64. Schifano F., Di Furia L., Forza G., Minicuci N., and Bricolo R. MDMA ('ecstasy') consumption in the context of polydrug abuse: a report on 150 patients. Drug Alcohol Depend. 52 (1998) 85-90
65. Schifano F., Oyefeso A., Corkery J., Cobain K., Jambert-Gray R., Martinotti G., and Ghodse A.H. Death rates from ecstasy (MDMA, MDA) and polydrug use in England and Wales 1996-2002. Hum. Psychopharmacol. 18 (2003) 519-524
66. Seelig G.F., Simondsen R.P., and Meister A. Reversible dissociation of gamma-glutamylcysteine synthetase into two subunits. J. Biol. Chem. 259 (1984) 9345-9347
67. Skulachev V.P. Bioenergetic aspects of apoptosis, necrosis and mitoptosis. Apoptosis 11 (2006) 473-485
68. Smart R.G., and Ogborne A.C. Drug use and drinking among students in 36 countries. Addict. Behav. 25 (2000) 455-460
69. Topp L., Hando J., Dillon P., Roche A., and Solowij N. Ecstasy use in Australia: patterns of use and associated harm. Drug Alcohol Depend. 55 (1999) 105-115
70. Tossmann P., Boldt S., and Tensil M.D. The use of drugs within the techno party scene in European metropolitan cities. Eur. Addict. Res. 7 (2001) 2-23
71. van Ommen B., Ploemen J.H., Bogaards J.J., Monks T.J., Gau S.S., and van Bladeren P.J. Irreversible inhibition of rat glutathione S-transferase 1-1 by quinones and their glutathione conjugates. Structure-activity relationship and mechanism. Biochem. J. 276 (1991) 661-666
72. Winstock A.R., Griffiths P., and Stewart D. Drugs and the dance music scene: a survey of current drug use patterns among a sample of dance music enthusiasts in the UK. Drug Alcohol Depend. 64 (2001) 9-17