Recovery of choline oxidase activity by in vitro recombination of individual segments

Applied Microbiology and Biotechnology

Volumn 81, Issue 2, 2008, Pages 275-282

  Heinze, Birgit ^a   Hoven, Nina ^b   O'Connell, Timothy ^b   Maurer, Karl Heinz ^b   Bartsch, Sebastian ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b HENKEL KGAA   (Germany)

Author keywords

Chimeragenesis; Choline oxidase; In vitro recombination

Indexed keywords

ENZYMES; ESCHERICHIA COLI; GENE ENCODING;

ARTHROBACTER GLOBIFORMIS; ARTHROBACTER PASCENS; CHIMERAGENESIS; CHIMERIC ENZYMES; CHOLINE OXIDASE; CHOLINE OXIDASES; ENCODING GENES; IN VITRO; IN VITRO RECOMBINATION; SEQUENCE ALIGNMENTS; SUBSTRATE SPECIFICITIES; SYNTHETIC GENES;

ENZYME ACTIVITY;

2 METHYLAMINOETHANOL; ALCOHOL DERIVATIVE; CHIMERIC PROTEIN; CHOLINE OXIDASE; DEANOL; ENZYME VARIANT; RECOMBINANT ENZYME;

BACTERIUM; ENZYME ACTIVITY; MUTATION; RECOMBINATION;

AMINO ACID SUBSTITUTION; ARTHROBACTER; ARTHROBACTER GLOBIFORMIS; ARTHROBACTER PASCENS; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; GENE EXPRESSION; GENE MUTATION; HYDROGEN BOND; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; SEQUENCE ALIGNMENT; THERMOSTABILITY;

ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTHROBACTER; CLONING, MOLECULAR; DEANOL; ENZYME STABILITY; ESCHERICHIA COLI; ETHANOLAMINES; HEXANOLS; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE; RECOMBINATION, GENETIC; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; TEMPERATURE;

ARTHROBACTER GLOBIFORMIS; ARTHROBACTER PASCENS; ESCHERICHIA COLI;

EID: 55649109629     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1605-0     Document Type: Article

References (30)

1. RCJ Bateman JA Evans 1995 Using the glucose oxidase/peroxidase system in enzyme kinetics J Chem Edu 72 A240
2. PL Bergquist RA Reeves MD Gibbs 2005 Degenerate oligonucleotide gene shuffling (DOGS) and random drift mutagenesis (RNDM): Two complementary techniques for enzyme evolution Biomol Eng 22 63 72
3. P Deshnium DA Los H Hayashi L Mustardy N Murata 1995 Transformation of synechococcus with a gene for choline oxidase enhances tolerance to saltstress Plant Mol Biol 29 897 907
4. PJ Dillon CA Rosen 1990 A rapid method for the construction of synthetic genes using the polymerase chain reaction BioTechniques 9 298 300
5. F Fan M Ghanem G Gadda 2004 Cloning, sequence analysis, and purification of choline oxidase from Arthrobacter globiformis: a bacterial enzyme involved in osmotic stress tolerance Arch Biochem Biophys 421 149 158
6. M Ghanem G Gadda 2005 On the catalytic role of the conserved active site residue His466 of choline oxidase Biochemistry 44 893 904
7. MD Gibbs KM Nevalainen PL Bergquist 2001 Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling Gene 271 13 20
8. DV Goeddel DG Kleid F Bolivar HL Heyneker DG Yansura R Crea T Hirose A Kraszewski K Itakura AD Riggs 1979 Expression in Escherichia coli of chemically synthesized genes for human insulin Proc Natl Acad Sci USA 76 106 110
9. HL Heyneker J Shine HM Goodman HW Boyer J Rosenberg RE Dickerson SA Narang S-Y Lin AD Riggs 1976 Synthetic lac operator DNA is functional in vivo Nature 263 748 752
10. JV Hoang G Gadda 2007 Trapping choline oxidase in a nonfunctional conformation by freezing at low pH Proteins: Struct, Funct, Bioinf 66 611 620
11. S Ikuta S Imamura H Misaki Y Horiuti 1977 Purification and characterization of choline oxidase from Arthrobacter globiformis J Biochem 82 1741 1749
12. K Itakura T Hirose R Crea AD Riggs HL Heyneker F Bolivar HW Boyer 1977 Expression in E. coli of a chemically synthesized gene for the hormone somatostatin Science 198 1056 1063
13. Keller WA, Datla RSS, Dong J-Z, Georges F, Hussain AAK, Selvaraj G (2004) Method and compositions for modifying levels of secondary metabolic compounds in plant. United States Patent 7279619
14. E Krieger T Darden SB Nabuurs A Finkelstein G Vriend 2004 Making optimal use of empirical energy functions: Force-field parameterization in crystal space Proteins Struct Funct Genet 57 678 683
15. Lountos GT (2005) Structural and mechanistic insights from high resolution crystal structures of the toluene-4-monooxygenase catalytic effector protein, NAD(P)H oxidase and choline oxidase. Georgia State University, Atlanta
16. W Mandecki TJ Bolling 1988 FokI method of gene synthesis Gene 68 101 107
17. SD McNeil ML Nuccio AD Hanson 1999 Betaines and related osmoprotectants. Targets for metabolic engineering of stress resistance Plant Physiol 120 945 949
18. Michel B, Baharoglu Z, Lestini R (2007) Genetics of recombination in the model bacterium Escherichia coli. In: Aguilera A, Rothstein R (eds) Molecular genetics of recombination. vol. 17. Springer, Berlin
19. KL Morley RJ Kazlauskas 2005 Improving enzyme properties: when are closer mutations better? Trends Biotechnol 23 231 237
20. M Ohta-Fukuyama Y Miyake S Emi T Yamano 1980 Identification and properties of the prosthetic group of choline oxidase from Alcaligenes sp. J Biochem 88 197 203
21. KA Pikal-Cleland N Rodríguez-Hornedo GL Amidon JF Carpenter 2000 Protein denaturation during freezing and thawing in phosphate buffer systems: Monomeric and tetrameric β-galactosidase Arch Biochem Biophys 384 398 406
22. O Quaye GT Lountos F Fan AM Orville G Gadda 2008 Role of Glu312 in binding and positioning of the substrate for the hydride transfer reaction in choline oxidase Biochem 47 243 256
23. T Rand T Halkier OC Hansen 2003 Structural characterization and mapping of the covalently linked FAD cofactor in choline oxidase from Arthrobacter globiformis Biochemistry 42 7188 7194
24. KL Rozwadowski GG Khachatourians G Selvaraj 1991 Choline oxidase, a catabolic enzyme in Arthrobacter pascens, facilitates adaption to osmotic stress in Escherichia coli J Bacteriol 173 472 478
25. WPC Stemmer 1994 DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution Proc Natl Acad Sci USA 91 10747 10751
26. WPC Stemmer 1994 Rapid evolution of a protein in vitro by DNA shuffling Nature 370 389 391
27. WPC Stemmer A Crameri KD Ha TM Brennan HL Heyneker 1995 Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides Gene 164 49 53
28. R Velasco-García VJ Zaldívar-Machorro Carlos-Mújica- Jiménez L González-Segura RA Muňoz-Clares 2006 Disulfiram irreversibly aggregates betaine aldehyde dehydrogenase-a potential target for antimicrobial agents against Pseudomonas aeruginosa Biochem Biophys Res Commun 341 408 415
29. J Wang P Cieplak PA Kollman 2000 How well does a restrained electrostatic potential (RESP) model perform in calculating conformational energies of organic and biological molecules J Comput Chem 21 1049 1074
30. J Wang RM Wolf JW Caldwell PA Kollman DA Case 2005 Development and testing of a general amber force field J Comput Chem 26 114