GFP-expressing locus ceruleus neurons from Prp57 transgenic mice exhibit CO2/H+ responses in primary cell culture

Journal of Applied Physiology

Volumn 105, Issue 4, 2008, Pages 1301-1311

  Johnson, Shereé M ^a,c   Haxhiu, Musa A ^b   Richerson, George B ^c  

^a HOWARD UNIVERSITY COLLEGE OF MEDICINE   (United States)

^b CASE WESTERN RESERVE UNIVERSITY   (United States)

^c YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Carbon dioxide; Central respiratory chemoreceptors; CO2 H+ chemosensitivity; pH response; Primary cell culture

Indexed keywords

4 AMINOBUTYRIC ACID RECEPTOR BLOCKING AGENT; CARBON DIOXIDE; GLUTAMATE RECEPTOR ANTAGONIST; GREEN FLUORESCENT PROTEIN; PROTON; TYROSINE 3 MONOOXYGENASE; CATECHOLAMINE;

ACID BASE BALANCE; ACIDOSIS; ALKALOSIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CHEMOSENSITIVITY; CONTROLLED STUDY; HYPERCAPNIA; IMMUNOFLUORESCENCE; LOCUS CERULEUS; MEMBRANE POTENTIAL; MOUSE; NERVE CELL; NONHUMAN; PATCH CLAMP; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRANSGENIC ANIMAL; ACTION POTENTIAL; ANIMAL; CHEMORECEPTOR; CYTOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; METABOLISM; TIME; TRANSGENIC MOUSE;

ACTION POTENTIALS; ANIMALS; CARBON DIOXIDE; CATECHOLAMINES; CELLS, CULTURED; CHEMORECEPTORS; FLUORESCENT ANTIBODY TECHNIQUE; GREEN FLUORESCENT PROTEINS; HYDROGEN-ION CONCENTRATION; LOCUS COERULEUS; MICE; MICE, TRANSGENIC; NEURONS; PATCH-CLAMP TECHNIQUES; TIME FACTORS; TYROSINE 3-MONOOXYGENASE;

EID: 55449110422     PISSN: 87507587     EISSN: 15221601     Source Type: Journal    
DOI: 10.1152/japplphysiol.90414.2008     Document Type: Article

References (75)

1. Aghajanian GK, Cedarbaum JM, Wang RY. Evidence for norepinephrine-mediated collateral inhibition of locus coeruleus neurons. Brain Res 136: 570-577, 1977.
2. Arata A, Onimaru H, Homma I. The adrenergic modulation of firings of respiratory rhythm-generating neurons in medulla-spinal cord preparation from newborn rat. Exp Brain Res 119: 399-408, 1998.
3. Aston-Jones G, Foote SL, Segal M. Impulse conduction properties of noradrenergic locus coeruleus axons projecting to monkey cerebrocortex. Neuroscience 15: 765-777, 1985.
4. Ballantyne D, Scheid P. Central chemosensitivity of respiration: a brief overview. Respir Physiol 129: 5-12, 2001.
5. Ballantyne D, Scheid P. Central respiratory chemosensitivity: cellular and network mechanisms. Adv Exp Med Biol 17-26, 2001.
6. Bernard DG, Li A, Nattie EE. Evidence for central chemoreception in the midline raphe. J Appl Physiol 80: 108-115, 1996.
7. 2 drive to breathing. Pflügers Arch 455: 1119 -1128, 2008.
8. Bou-Flores C, Lajard AM, Monteau R, De ME, Seif I, Lanoir J, Hilaire G. Abnormal phrenic motoneuron activity and morphology in neonatal monoamine oxidase A-deficient transgenic mice: possible role of a serotonin excess. J Neurosci 20: 4646-4656, 2000.
9. Coates EL, Li A, Nattie EE. Widespread sites of brain stem ventilatory chemoreceptors. J Appl Physiol 75: 5-14, 1993.
10. 2. J Physiol 441: 433-451, 1991.
11. 2 chemoreception and respiratory control. Am J Physiol Lung Cell Mol Physiol 283: L665-L670, 2002.
12. Dean JB, Bayliss PA, Eriksson LI, Lawing WL, Millhorn DE. Depolarization and stimulation of neurons in nucleus tractus solitarii by carbon dioxide does not require chemical synaptic input. Neuroscience 36: 207-216, 1990.
13. Dean JB, Mulkey DK, Garcia AJ, III, Putnam RW, Henderson RA, III. Neuronal sensitivity to hyperoxia, hypercapnia, and inert gases at hyperbaric pressures. J Appl Physiol 95: 883-909, 2003.
14. Dean JB, Mulkey DK, Henderson RA III, Potter SJ, Putnam RW. Hyperoxia, reactive oxygen species, and hyperventilation: oxygen sensitivity of brain stem neurons. J Appl Physiol 96: 784-791, 2004.
15. Dillon GH, Waldrop TG. In vitro responses of caudal hypothalamic neurons to hypoxia and hypercapnia. Neuroscience 51: 941-950, 1992.
16. Dillon GH, Waldrop TG. Responses of feline caudal hypothalamic cardiorespiratory neurons to hypoxia and hypercapnia. Exp Brain Res 96: 260-272, 1993.
17. Dobbins EG, Feldman JL. Brainstem network controlling descending drive to phrenic motoneurons in rat. J Comp Neurol 347: 64-68, 1994.
18. Elam M, Yao T, Thoren P, Svensson TH. Hypercapnia and hypoxia: chemoreceptor-mediated control of locus coeruleus neurons and splanchnic, sympathetic nerves. Brain Res 222: 373-381, 1981.
19. Ennis M, ston-Jones G. Evidence for self- and neighbor-mediated postactivation inhibition of locus coeruleus neurons. Brain Res 374: 299-305, 1986.
20. 2 chemosensory excitability in the retrotrapezoid nucleus of rodents. Adv Exp Med Biol 605: 317-321, 2008.
21. Feldman JL, Mitchell GS, Nattie EE. Breathing: rhythmicity, plasticity, chemosensitivity. Annu Rev Neurosci 26: 239-266, 2003.
22. Filosa JA, Dean JB, Putnam RW. Role of intracellular and extracellular pH in the chemosensitive response of rat locus coeruleus neurones. J Physiol 541: 493-509, 2002.
23. 2+ channels. Am J Physiol Cell Physiol 284: C145-C155, 2003.
24. Fitzgerald SC, Willis MA, Yu C, Rigatto H. In search of the central respiratory neurons: I. Dissociated cell cultures of respiratory areas from the upper medulla. J Neurosci Res 33: 579-589, 1992.
25. +-sensitivity and pattern of discharge of neurons in the chemosensitive areas of the ventral medulla oblongata of rats in vitro. Pflügers Arch 388: 53-61, 1980.
26. Gray PA, Janczewski WA, Mellen N, McCrimmon DR, Feldman JL. Normal breathing requires preBotzinger complex neurokinin-1 receptor-expressing neurons. Nat Neurosci 4: 927-930, 2001.
27. Guyenet PG, Stornetta RL, Bayliss DA, Mulkey DK. Retrotrapezoid nucleus: a litmus test for the identification of cental chemoreceptors. Exp Physiol 90: 247-253, 2005.
28. Hartzler LK, Dean JB, Putnam RW. The chemosensitive response of neurons from the locus coeruleus (LC) to hypercapnic acidosis with clamped intracellular pH. Adv Exp Med Biol 605: 333-337, 2008.
29. 2-induced c-fos expression in the CNS catecholaminergic neurons. Respir Physiol 105: 35-45, 1996.
30. Hewitt A, Barrie R, Graham M, Bogus K, Leiter JC, Erlichman JS. Ventilatory effects of gap junction blockade in the RTN in awake rats. Am J Physiol Regul Integr Comp Physiol 287: R1407-R1418, 2004.
31. Hilaire G, Duron B. Maturation of the mammalian respiratory system. Physiol Rev 79: 325-360, 1999.
32. Hobson JA, McCarley R, Pivik RT, Freedman R. Selective firing by cat pontine brain stem neurons in desynchronized sleep. J Neurophysiol 37: 497-511, 1974.
33. + responses in primary cell culture. FASEB J 21: lb571, 2007.
34. Johnson SM, Trouth CO, Smith JC. Chemosensitivity of respiratory pacemaker neurons in the pre-Botzinger complex in vitro. Neuroscience 24: 875, 1998.
35. Li A, Nattie E. Catecholamine neurones in rats modulate sleep, breathing, central chemoreception and breathing variability. J Physiol 570: 385-396, 2006.
36. Miles R. Does low pH stimulate central chemoreceptors located near the ventral medullary surface? Brain Res 271: 349-353, 1983.
37. Mitchell RA, Loeschcke HH, Massion WH, Severinghaus JW. Respiratory responses mediated through superficial chemosensitive areas on the medulla. J Appl Physiol 18: 523-533, 1963.
38. +-sensitive neurons in rat brain stem slices. J Appl Physiol 95: 910-921, 2003.
39. + exchange and increases excitability of solitary complex neurons from rat brain slices. Am J Physiol Cell Physiol 286: C940-C951, 2004.
40. Mulkey DK, Henderson RA III, Ritucci NA, Putnam RW, Dean JB. Chemical oxidants acidify solitary complex (SC) neurons in the rat. Undersea Hyperb Med 31: 107-111, 2004.
41. Nattie EE, Li A. Central chemoreception in the region of the ventral respiratory group in the rat. J Appl Physiol 81: 1987-1995, 1996.
42. Nichols NL, Hartzler LK, Conrad SC, Dean JB, Putnam RW. Intrinsic chemosensitivity of individual nucleus tractus solitarius (NTS) and locus coeruleus (LC) neurons from neonatal rats. Adv Exp Med Biol 605: 348-352, 2008.
43. Onimaru H, Arata A, Homma I. Neuronal mechanisms of respiratory rhythm generation: an approach using in vitro preparation. Jpn J Physiol 47: 385-403, 1997.
44. Oyamada Y, Andrzejewski M, Muckenhoff K, Scheid P, Ballantyne D. Locus coeruleus neurones in vitro: pH-sensitive oscillations of membrane potential in an electrically coupled network. Respir Physiol 118: 131-147, 1999.
45. Oyamada Y, Ballantyne D, Muckenhoff K, Scheid P. Respiration-modulated membrane potential and chemosensitivity of locus coeruleus neurones in the in vitro brainstem-spinal cord of the neonatal rat. J Physiol 513: 381-398, 1998.
46. Pineda J, Aghajanian GK. Carbon dioxide regulates the tonic activity of locus coeruleus neurons by modulating a proton- and polyamine-sensitive inward rectifier potassium current. Neuroscience 77: 723-743, 1997.
47. 2 chemosensitivity. Respir Physiol Neurobiol 149: 165-179, 2005.
48. 2 and acid signaling in chemosensitive neurons. Am J Physiol Cell Physiol 287: C1493-C1526, 2004.
49. 2 of neurons in the rostral ventral medulla in vitro. J Neurophysiol 73: 933-944, 1995.
50. Richerson GB, Wang W, Hodges MR, Dohle CI, ez-Sampedro A. Homing in on the specific phenotype(s) of central respiratory chemoreceptors. Exp Physiol 90: 259-266, 2005.
51. Richerson GB, Wang W, Tiwari J, Bradley SR. Chemosensitivity of serotonergic neurons in the rostral ventral medulla. Respir Physiol 129: 175-189, 2001.
52. 2 in the upper medulla: dose response and effects of mediators. Pediatr Pulmonol 30: 359-367, 2000.
53. Ritucci NA, Chambers-Kersh L, Dean JB, Putnam RW. Intracellular pH regulation in neurons from chemosensitive and nonchemosensitive areas of the medulla. Am J Physiol Regul Integr Comp Physiol 275: R1152-R1163, 1998.
54. Ritucci NA, Dean JB, Putnam RW. Somatic vs. dendritic responses to hypercapnia in chemosensitive locus coeruleus neurons from neonatal rats. Am J Physiol Cell Physiol 289: C1094-C1104, 2005.
55. Ritucci NA, Dean JB, Putnam RW. Intracellular pH response to hypercapnia in neurons from chemosensitive areas of the medulla. Am J Physiol Regul Integr Comp Physiol 273: R433-R441, 1997.
56. Ritucci NA, Erlichman JS, Leiter JC, Putnam RW. Response of membrane potential and intracellular pH to hypercapnia in neurons and astrocytes from rat retrotrapezoid nucleus. Am J Physiol Regul Integr Comp Physiol 289: R851-R861, 2005.
57. + ion concentration in small areas of the ventral medullary surface. Respir Physiol 10: 198-212, 1970.
58. 2-chemosensitive brainstem regions in rat. Respir Physiol Neurobiol 139: 1-20, 2003.
59. Solomon IC, Chon KH, Rodriguez MN. Blockade of brain stem gap junctions increases phrenic burst frequency and reduces phrenic burst synchronization in adult rat. J Neurophysiol 89: 135-149, 2003.
60. 2- chemoreception and respiratory control. Respir Physiol Neurobiol 131: 155-173, 2002.
61. + chemoreception in the cat pre-Botzinger complex in vivo. J Appl Physiol 88: 1996-2007, 2000.
62. 2-chemosensitive brainstem regions in rat. Respir Physiol 129: 101-121, 2001.
63. Stunden CE, Filosa JA, Garcia AJ, Dean JB, Putnam RW. Development of in vivo ventilatory and single chemosensitive neuron responses to hypercapnia in rats. Respir Physiol 127: 135-155, 2001.
64. 2 chemosensitivity versus wide sensing spectrum: a paradoxical problem and its solutions in cultured brainstem neurons. J Physiol 578: 831-841, 2007.
65. Thoby-Brisson M, Ramirez JM. Role of inspiratory pacemaker neurons in mediating the hypoxic response of the respiratory network in vitro. J Neurosci 20: 5858-5866, 2000.
66. van den Pol AN, Ghosh PK. Selective neuronal expression of green fluorescent protein with cytomegalovirus promoter reveals entire neuronal arbor in transgenic mice. J Neurosci 18: 10640-10651, 1998.
67. van den Pol AN, Ghosh PK, Liu RJ, Li Y, Aghajanian GK, Gao XB. Hypocretin (orexin) enhances neuron activity and cell synchrony in developing mouse GFP-expressing locus coeruleus. J Physiol 541: 169-185, 2002.
68. Viemari JC, Bevengut M, Burnet H, Coulon P, Pequignot JM, Tiveron MC, Hilaire G. Phox2a gene, A6 neurons, and noradrenaline are essential for development of normal respiratory rhythm in mice. J Neurosci 24: 928-937, 2004.
69. Wang F, Xiao C, Ye JH. Taurine activates excitatory non-synaptic glycine receptors on dopamine neurones in ventral tegmental area of young rats. J Physiol 565: 503-516, 2005.
70. 2. J Physiol 540: 951-970, 2002.
71. Wang W, Pizzonia JH, Richerson GB. Chemosensitivity of rat medullary raphe neurones in primary tissue culture. J Physiol 511: 433-450, 1998.
72. Wang W, Richerson GB. Development of chemosensitivity of rat medullary raphe neurons. Neuroscience 90: 1001-1011, 1999.
73. Wang W, Richerson GB. Chemosensitivity of non-respiratory rat CNS neurons in tissue culture. Brain Res 860: 119-129, 2000.
74. Wang W, Tiwari JK, Bradley SR, Zaykin RV, Richerson GB. Acidosis-stimulated neurons of the medullary raphe are serotonergic. J Neurophysiol 85: 2224-2235, 2001.
75. 2. Proc Natl Acad Sci USA 104: 10685-10690, 2007.