Extraction of RNA from cheese without prior separation of microbial cells

Applied and Environmental Microbiology

Volumn 74, Issue 18, 2008, Pages 5724-5730

  Monnet, Christophe ^a,c   Ulvé, Vincent ^b   Sarthou, Anne Sophie ^a   Irlinger, Françoise ^a  

^a Paris Saclay University   (France)

^b UMR1253   (France)

^c AGROPARISTECH   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CHEESES; EXTRACTION; POLYMERASE CHAIN REACTION; RNA; TRANSCRIPTION;

CELLULAR PROCESS; GENE EXPRESSION STUDIES; INCUBATION PERIODS; LACTOCOCCUS LACTIS; REPRODUCIBILITIES; REVERSE TRANSCRIPTION PCR; RNA CONCENTRATION; TRANSCRIPT ANALYSIS;

NUCLEIC ACIDS;

GENOMIC DNA; MESSENGER RNA; RNA 16S; RNA 23S; BACTERIAL RNA;

BACTERIUM; GENE EXPRESSION; MICROBIAL COMMUNITY; MILK; OSMOSIS; POLYMERASE CHAIN REACTION; RNA; SEPARATION;

ACIDITY; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL STRAIN; CELL SEPARATION; CHEESE; GENE EXPRESSION; HEAT; INCUBATION TEMPERATURE; INCUBATION TIME; LACTOCOCCUS LACTIS; NONHUMAN; OSMOTIC STRESS; QUANTITATIVE GENETICS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA EXTRACTION; ANALYSIS; FOOD CONTROL; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY;

LACTOCOCCUS LACTIS;

ANALYTIC SAMPLE PREPARATION METHODS; CHEESE; FOOD MICROBIOLOGY; LACTOCOCCUS LACTIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL;

EID: 52649167944     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00716-08     Document Type: Article

References (24)

1. Baruzzi, F., A. Matarante, L. Caputo, and M. Morea. 2005. Development of a culture-independent polymerase chain reaction-based assay for the detection of lactobacilli in stretched cheese. J. Rapid Methods Automat. Microbiol. 13:177-192.
2. Bonaïti, C., S. Parayre, and F. Irlinger. 2006. Novel extraction strategy of ribosomal RNA and genomic DNA from cheese for PCR-based investigations. Int. J. Food Microbiol. 107:171-179.
3. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
4. Coppola, S., G. Blaiotta, D. Ercolini, and G. Moschetti. 2001. Molecular evaluation of microbial diversity occurring in different types of Mozzarella cheese. J. Appl. Microbiol. 90:414-420.
5. Fernandez, M., B. del Rio, D. M. Linares, M. C. Martin, and M. A. Alvarez. 2006. Real-time polymerase chain reaction for quantitative detection of histamine-producing bacteria: use in cheese production. J. Dairy Sci. 89:3763-3769.
6. Fukushima, H., K. Katsube, Y. Hata, R. Kishi, and S. Fujiwara. 2007. Rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time PCR. Appl. Environ. Microbiol. 73:92-100.
7. Hannon, J. A., S. M. Deutsch, M. N. Madec, J. Y. Gassi, M. P. Chapot-Chartier, and S. Lortal. 2006. Lysis of starters in UF cheeses: behaviour of mesophilic lactococci and thermophilic lactobacilli. Int. Dairy J. 16:324-334.
8. Hein, I., A. Lehner, P. Rieck, K. Klein, E. Brandl, and M. Wagner. 2001. Comparison of different approaches to quantify Staphylococcus aureus cells by real-time quantitative PCR and application of this technique for examination of cheese. Appl. Environ. Microbiol. 67:3122-3126.
9. Henri-Dubernet, S., N. Desmasures, and M. Guéguen. 2004. Culture-dependent and culture-independent methods for molecular analysis of the diversity of lactobacilli in "Camembert de Normandie" cheese. Lait 84:179-189.
10. McKillip, J. L., L. A. Jaykus, and M. A. Drake. 2000. A comparison of methods for the detection of Escherichia coli O157:H7 from artificially-contaminated dairy products using PCR. J. Appl. Microbiol. 89:49-55.
11. Monnet, C., K. Correia, A.-S. Sarthou, and F. Irlinger. 2006. Quantitative detection of Corynebacterium casei in cheese by real-time PCR. Appl. Environ. Microbiol. 72:6972-6979.
12. Ogier, J.-C., V. Lafarge, V. Girard, A. Rault, V. Maladen, A. Gruss, J.-Y. Leveau, and A. Delacroix-Buchet. 2004. Molecular fingerprinting of dairy microbial ecosystems by use of temporal temperature and denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 70:5628-5643.
13. Ogier, J.-C., O. Son, A. Gruss, P. Tailliez, and A. Delacroix-Buchet. 2002. Identification of the bacterial microflora in dairy products by temporal temperature gradient gel electrophoresis. Appl. Environ. Microbiol. 68:3691-3701.
14. Pfaffl, M. W. 2004. Quantification strategies in real-time PCR, p. 87-112. In S. A. Bustin (ed.), A-Z of quantitative PCR. International University Line, La Jolla, CA.
15. Rademaker, J. L. W., J. D. Hoolwerf, A. A. Wagendorp, and M. C. te Giffel. 2006. Assessment of microbial population dynamics during yoghurt and hard cheese fermentation and ripening by DNA population fingerprinting. Int. Dairy J. 16:457-466.
16. Randazzo, C. L., S. Torriani, A. D. L. Akkermans, W. M. de Vos, and E. E. Vaughan. 2002. Diversity, dynamics, and activity of bacterial communities during production of an artisanal Sicilian cheese as evaluated by 16S rRNA analysis. Appl. Environ. Microbiol. 68:1882-1892.
17. Rantsiou, K., G. Comi, and L. Cocolin. 2004. The rpoB gene as a target for PCR-DGGE analysis to follow lactic acid bacterial population dynamics during food fermentations. Food Microbiol. 21:481-487.
18. Rudi, K., K. Naterstad, S. M. Dromtorp, and H. Holo. 2005. Detection of viable and dead Listeria monocytogenes on gouda-like cheeses by real-time PCR. Lett. Appl. Microbiol. 40:301-306.
19. Sperandio, B., P. Polard, D. S. Ehrlich, P. Renault, and E. Guedon. 2005. Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403. J. Bacteriol. 187:3762-3778.
20. Stevens, K. A., and L. A. Jaykus. 2004. Direct detection of bacterial pathogens in representative dairy products using a combined bacterial concentration-PCR approach. J. Appl. Microbiol. 97:1115-1122.
21. Terzaghi, B. E., and W. E. Sandine. 1975. Improved medium for lactic streptococci and their bacteriophages. Appl. Microbiol. 29:807-813.
22. Ulvé, V. M., C. Monnet, F. Valence, J. Fauquant, H. Falentin, and S. Lortal. RNA extraction from cheese for analysis of in situ gene expression of Lactococcus lactis. J. Appl. Microbiol., in press.
23. Wouters, J. A., H. Frenkiel, W. M. de Vos, O. P. Kuipers, and T. Abee. 2001. Cold shock proteins of Lactococcus lactis MG1363 are involved in cryoprotection and in the production of cold-induced proteins. Appl. Environ. Microbiol. 67:5171-5178.
24. Xie, Y., L.-S. Chou, A. Cutler, and B. Weimer. 2004. DNA macroarray profiling of Lactococcus lactis subsp. lactis IL1403 gene expression during environmental stresses. Appl. Environ. Microbiol. 70:6738-6747.