Secretion of human interferon alpha 2b by Streptomyces lividans

Applied Microbiology and Biotechnology

Volumn 58, Issue 2, 2002, Pages 189-194

  Pimienta, E ^a   Fando, R ^b   Sanchez J ^c   Vallin, C ^a  

^a Centro de Quimica Farmaceutica   (Cuba)

^b NATL CENTER FOR SCIENTIFIC RESEARCH   (Cuba)

^c CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

[No Author keywords available]

Indexed keywords

RECOMBINANT ALPHA2B INTERFERON; SIGNAL PEPTIDE;

MICROBIOLOGY;

ARTICLE; BACTERIUM CULTURE; CELL LYSATE; CONTROLLED STUDY; CULTURE MEDIUM; CYTOKINE RELEASE; MYCELIUM; NONHUMAN; PROTEIN DEGRADATION; PROTEIN PROCESSING; RECOMBINANT PLASMID; STREPTOMYCES LIVIDANS;

CULTURE MEDIA; CULTURE MEDIA, CONDITIONED; HUMANS; INTERFERON ALFA-2B; LIPASE; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; STREPTOMYCES;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); STREPTOMYCES; STREPTOMYCES EXFOLIATUS; STREPTOMYCES LIVIDANS; UNCULTURED ACTINOMYCETE;

EID: 0036158956     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-001-0873-8     Document Type: Article

References (29)

1. Determinación de la actividad de interferon
2. Regulation of extracellular protease production in Streptomyces clavuligerus
3. The mRNA for the 23S rRNA methylase encoded by the ermE gene of Saccharopolyspora erythraea is translated in the absence of a conventional ribosome-binding site
4. Isolation and characterization of two genes encoding proteases associated with the mycelium of Streptomyces lividans 66
5. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding
6. Secretion of heterologous proteins in Streptomyces lividans
7. Genetic instability and DNA amplification in Streptomyces lividans 66
8. Soluble forms of the human T cell receptor CD4 are efficiently expressed by S. lividans
9. Production and secretion of proteins of Streptomycetes
10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
11. Evaluation of a novel subtilisin inhibitor gene and mutant derivatives for the expression and secretion of mouse tumour necrosis factor alpha by S. lividans
12. Accumulation of bldA-specific tRNA is temporally regulated in Streptomyces coelicolor A3(2)
13. Use of the pre-pro part of Staphylococcus hyicus lipase as a carrier for secretion of Escherichia coli outer membrane protein A (OmpA) prevents proteolytic degradation of OmpA by cell-associated protease(s) in two different Gram-positive bacteria
14. Protein secretion in Streptomycetes
15. Synthesis in E. coli of a polypeptide with human leukocyte interferon activity
16. Cloning, characterization, and expression in Streptomyces lividans 66 of an extracellular lipase-encoding gene from Streptomyces sp. M11
17. Tratamientos analíticos de muestras de alfa h-rec. para su cuantificación por ELISA
18. Cloning and expression in biologically active form of the gene for human interferon α2 Streptomyces lividans
19. Elimination of an HuIFNα readthrough species, produced in Escherichia coli, by replacing its natural translational stop signal
20. Promoter constructions for efficient secretion expression in Streptomyces lividans
21. bldA-dependent expression of the Streptomyces exfoliatus M11 lipase gene (lipA) is mediated by the product of a contiguous gene, lipR, encoding a putative transcriptional activator
22. New multifunctional Escherichia coli-Streptomyces shuttle vectors allow blue-white screening on XGal plates
23. Codon usage in the G+C-rich Streptomyces genome
24. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors