Efficient expression and purification of human interferon alpha2b in the methylotrophic yeast, Pichia pastoris

Protein Expression and Purification

Volumn 54, Issue 2, 2007, Pages 220-226

  Shi, Linmei ^a,c   Wang, Dongming ^a,c   Chan, Weiguang ^b,c   Cheng, Luzi ^b,c  

^a Lishui Vocational and Technical College   (China)

^b ZHEJIANG UNIVERSITY OF TECHNOLOGY   (China)

^c WOMEN S HOSPITAL OF NANJING MEDICAL UNIVERSITY   (China)

Author keywords

hIFN 2b; Pichia pastoris; Protein purification; Secretion expression

Indexed keywords

ALPHA2B INTERFERON;

AMINO ACID SEQUENCE; ARTICLE; BIOASSAY; BIOSYNTHESIS; DRUG EFFECT; ENCEPHALOMYOCARDITIS VIRUS; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; PICHIA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; BIOLOGICAL ASSAY; ENCEPHALOMYOCARDITIS VIRUS; INTERFERON ALFA-2B; MASS SPECTROMETRY; PICHIA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

PICHIA PASTORIS;

EID: 34249087703     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.03.005     Document Type: Article

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