Green tea polyphenol epigallocatechin-3-gallate suppresses collagen production and proliferation in keloid fibroblasts via inhibition of the STAT3-signaling pathway

Journal of Investigative Dermatology

Volumn 128, Issue 10, 2008, Pages 2429-2441

  Park, Gyuman ^a   Yoon, Byung Sun ^b   Moon, Jai Hee ^b   Kim, Bona ^b   Jun, Eun Kyoung ^b   Oh, Sejong ^c   Kim, Hyunggee ^b   Song, Hea Joon ^a   Noh, Joo Young ^d   Oh, ChilHwan ^a,b   You, Seungkwon ^b  

^a Korea University College of Medicine   (South Korea)

^b Korea University   (South Korea)

^c Chonnam National University   (South Korea)

^d Gachon University College of Medicine   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

1,4 DIAMINO 1,4 BIS(2 AMINOPHENYLTHIO) 2,3 DICYANOBUTADIENE; 2 MORPHOLINO 8 PHENYLCHROMONE; COLLAGEN; DOXORUBICIN; EPIGALLOCATECHIN GALLATE; GREEN TEA EXTRACT; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE INHIBITOR; N BENZYL 2 CYANO 3 (3,4 DIHYDROXYPHENYL)ACRYLAMIDE; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHATIDYLINOSITOL 3 KINASE INHIBITOR; POLYPHENOL DERIVATIVE; SMAD2 PROTEIN; SMAD3 PROTEIN; STAT3 PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL CYCLE ARREST; CELL MIGRATION; CELL PROLIFERATION; CONTROLLED STUDY; DOSE RESPONSE; DRUG MECHANISM; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; FIBROBLAST; HUMAN; HUMAN CELL; IC 50; KELOID; MOUSE; NONHUMAN; PRIORITY JOURNAL; WOUND HEALING;

ANIMALS; CATECHIN; CELL CYCLE; CELL MOVEMENT; CELL PROLIFERATION; CELLS, CULTURED; COLLAGEN; FIBROBLASTS; FLAVONOIDS; HUMANS; KELOID; MICE; MICE, NUDE; MITOGEN-ACTIVATED PROTEIN KINASE KINASES; PHENOLS; PHOSPHATIDYLINOSITOL 3-KINASES; PROTEIN KINASE INHIBITORS; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; STAT3 TRANSCRIPTION FACTOR; TEA; TRITERPENES; WOUND HEALING;

EID: 51749085805     PISSN: 0022202X     EISSN: 15231747     Source Type: Journal    
DOI: 10.1038/jid.2008.103     Document Type: Article

References (52)

1. Ahn HY, Hadizadeh KR, Seul C, Yun YP, Vetter H, Sachinidis A (1999) Epigallocathechin-3 gallate selectively inhibits the PDGF-BB-induced intracellular signaling transduction pathway in vascular smooth muscle cells and inhibits transformation of sis-transfected NIH 3T3 fibroblasts and human glioblastoma cells (A172). Mol Biol Cell 10:1093-104
2. Al-Attar A, Mess S, Thomassen JM, Kauffman CL, Davison SP (2006) Keloid pathogenesis and treatment. Plast Reconstr Surg 117:286-300
3. Blaskovich MA, Sun J, Cantor A, Turkson J, Jove R, Sebti SM (2003) Discovery of JSI-124 (cucurbitacin I), a selective Janus kinase/signal transducer and activator of transcription 3 signaling pathway inhibitor with potent antitumor activity against human and murine cancer cells in mice. Cancer Res 63:1270-9
4. Blazer S, Khankin E, Segev Y, Ofir R, Yalon-Hacohen M, Kra-Oz Z et al. (2002) High glucose-induced replicative senescence: point of no return and effect of telomerase. Biochem Biophys Res Commun 296:93-101
5. Chen C, Shen G, Hebbar V, Hu R, Owuor ED, Kong AN (2003) Epigallocatechin-3-gallate-induced stress signals in HT-29 human colon adenocarcinoma cells. Carcinogenesis 24:1369-78
6. Chen SJ, Yuan W, Mori Y, Levenson A, Trojanowska M, Varga J (1999) Stimulation of type I collagen transcription in human skin fibroblasts by TGF-beta: involvement of Smad 3. J Invest Dermatol 112:49-57
7. Chen W, Fu XB, Ge SL, Sun XQ, Zhou G, Zhao ZL et al. (2004) Development of gene microarray in screening differently expressed genes in keloid and normal-control skin. Chin Med J (England) 117:877-81
8. Chiu AE, Chan JL, Kern DG, Kohler S, Rehmus WE, Kimball AB (2005) Double-blinded, placebo-controlled trial of green tea extracts in the clinical and histologic appearance of photoaging skin. Dermatol Surg 31:855-60, discussion 860
9. Darnell JE Jr (1997) STATs and gene regulation. Science 277:1630-5
10. DiPaola RS (2002) To arrest or not to G(2)-M cell-cycle arrest: commentary re: AK Tyagi et al., Silibinin strongly synergizes human prostate carcinoma DU145 cells to doxorubicin-induced growth inhibition, G(2)-M arrest, and apoptosis. Clin Cancer Res, 8: 3512-3519, 2002. Clin Cancer Res 8:3311-4
11. Dreosti IE, Wargovich MJ, Yang CS (1997) Inhibition of carcinogenesis by tea: the evidence from experimental studies. Crit Rev Food Sci Nutr 37:761-70
12. Ehrlich HP, Desmouliere A, Diegelmann RF, Cohen IK, Compton CC, Garner WL et al. (1994) Morphological and immunochemical differences between keloid and hypertrophic scar. Am J Pathol 145:105-13
13. Fujiwara M, Muragaki Y, Ooshima A (2005) Upregulation of transforming growth factor-beta1 and vascular endothelial growth factor in cultured keloid fibroblasts: relevance to angiogenic activity. Arch Dermatol Res 297:161-9
14. Gupta S, Hussain T, Mukhtar H (2003) Molecular pathway for (-)-epigallocatechin-3-gallate-induced cell cycle arrest and apoptosis of human prostate carcinoma cells. Arch Biochem Biophys 410:177-85
15. Hu J, Verkman AS (2006) Increased migration and metastatic potential of tumor cells expressing aquaporin water channels. FASEB J 20:1892-4
16. Hung CF, Huang TF, Chiang HS, Wu WB (2005) (-)-Epigallocatechin-3- gallate, a polyphenolic compound from green tea, inhibits fibroblast adhesion and migration through multiple mechanisms. J Cell Biochem 96:183-97
17. Isbrucker RA, Edwards JA, Wolz E, Davidovich A, Bausch J (2006) Safety studies on epigallocatechin gallate (EGCG) preparations. Part 2: dermal, acute and short-term toxicity studies. Food Chem Toxicol 44:636-50
18. Kada T, Kaneko K, Matsuzaki S, Matsuzaki T, Hara Y (1985) Detection and chemical identification of natural bio-antimutagens. A case of the green tea factor. Mutat Res 150:127-32
19. Kelly AP (2004) Medical and surgical therapies for keloids. Dermatol Ther 17:212-8
20. Khoo YT, Ong CT, Mukhopadhyay A, Han HC, Do DV, Lim IJ et al. (2006) Upregulation of secretory connective tissue growth factor (CTGF) in keratinocyte-fibroblast coculture contributes to keloid pathogenesis. J Cell Physiol 208:336-43
21. Kubota Y, Umegaki K, Tanaka N, Mizuno H, Nakamura K, Kunitomo M et al. (2002) Safety of dietary supplements: chronotropic and inotropic effects on isolated rat atria. Biol Pharm Bull 25:197-200
22. Kumar N, Shibata D, Helm J, Coppola D, Malafa M (2007) Green tea polyphenols in the prevention of colon cancer. Front Biosci 12:2309-15
23. Kuo YR, Wu WS, Jeng SF, Huang HC, Yang KD, Sacks JM et al. (2005) Activation of ERK and p38 kinase mediated keloid fibroblast apoptosis after flashlamp pulsed-dye laser treatment. Lasers Surg Med 36:31-7
24. Lim CP, Phan TT, Lim IJ, Cao X (2006) Stat3 contributes to keloid pathogenesis via promoting collagen production, cell proliferation and migration. Oncogene 25:5416-25
25. Lim IJ, Phan TT, Tan EK, Nguyen TT, Tran E, Longaker MT et al. (2003) Synchronous activation of ERK and phosphatidylinositol 3-kinase pathways is required for collagen and extracellular matrix production in keloids. J Biol Chem 278:40851-8
26. Liu JF, Zhang YM, Yi CX, Sun JM, Li WW (2004) [The expression and interaction of cyclin D1 and p16 in fibroblasts of pathologic scars]. Zhonghua Zheng Xing Wai Ke Za Zhi 20:265-7
27. Lorenzini A, Tresini M, Mawal-Dewan M, Frisoni L, Zhang H, Allen RG et al. (2002) Role of the Raf/MEK/ERK and the PI3K/Akt(PKB) pathways in fibroblast senescence. Exp Gerontol 37:1149-56
28. Luo S, Benathan M, Raffoul W, Panizzon RG, Egloff DV (2001) Abnormal balance between proliferation and apoptotic cell death in fibroblasts derived from keloid lesions. Plast Reconstr Surg 107:87-96
29. Marneros AG, Krieg T (2004) Keloids - clinical diagnosis, pathogenesis, and treatment options. J Dtsch Dermatol Ges 2:905-13
30. Masuda M, Suzui M, Lim JT, Deguchi A, Soh JW, Weinstein IB (2002) Epigallocatechin-3-gallate decreases VEGF production in head and neck and breast carcinoma cells by inhibiting EGFR-related pathways of signal transduction. J Exp Ther Oncol 2:350-9
31. Mirza AM, Kohn AD, Roth RA, McMahon M (2000) Oncogenic transformation of cells by a conditionally active form of the protein kinase Akt/PKB. Cell Growth Differ 11:279-92
32. Mutalik S (2005) Treatment of keloids and hypertrophic scars. Indian J Dermatol Venereol Leprol 71:3-8
33. Nowak KC, McCormack M, Koch RJ (2000) The effect of superpulsed carbon dioxide laser energy on keloid and normal dermal fibroblast secretion of growth factors: a serum-free study. Plast Reconstr Surg 105:2039-48
34. Paul B, Hayes CS, Kim A, Athar M, Gilmour SK (2005) Elevated polyamines lead to selective induction of apoptosis and inhibition of tumorigenesis by (-)-epigallocatechin-3-gallate (EGCG) in ODC/Ras transgenic mice. Carcinogenesis 26:119-24
35. Phan TT, Lim IJ, Aalami O, Lorget F, Khoo A, Tan EK et al. (2005) Smad3 signalling plays an important role in keloid pathogenesis via epithelial - mesenchymal interactions. J Pathol 207:232-42
36. Phan TT, Lim IJ, Chan SY, Tan EK, Lee ST, Longaker MT (2004) Suppression of transforming growth factor beta/smad signaling in keloid-derived fibroblasts by quercetin: implications for the treatment of excessive scars. J Trauma 57:1032-7
37. Rekha A (2004) Keloids - a frustrating hurdle in wound healing. Int Wound J 1:145-8
38. Sah JF, Balasubramanian S, Eckert RL, Rorke EA (2004) Epigallocatechin-3-gallate inhibits epidermal growth factor receptor signaling pathway. Evidence for direct inhibition of ERK1/2 and AKT kinases. J Biol Chem 279:12755-62
39. Satish L, Babu M, Tran KT, Hebda PA, Wells A (2004) Keloid fibroblast responsiveness to epidermal growth factor and activation of downstream intracellular signaling pathways. Wound Repair Regen 12:183-92
40. Sato M (2006) Upregulation of the Wnt/beta-catenin pathway induced by transforming growth factor-beta in hypertrophic scars and keloids. Acta Derm Venereol 86:300-7
41. Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK et al. (2001) Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat Res 480-481:243-68
42. Tan EM, Qin H, Kennedy SH, Rouda S, Fox JW 4th, Moore JH Jr (1995) Platelet-derived growth factors-AA and -BB regulate collagen and collagenase gene expression differentially in human fibroblasts. Biochem J 310(Part 2):585-8
43. Ullmann U, Haller J, Decourt JP, Girault N, Girault J, Richard-Caudron AS et al. (2003) A single ascending dose study of epigallocatechin gallate in healthy volunteers. J Int Med Res 31:88-101
44. Wang YC, Bachrach U (2002) The specific anti-cancer activity of green tea (-)-epigallocatechin-3-gallate (EGCG). Amino Acids 22:131-43
45. Wang Z, Ge L, Wang M, Carr BI (2006) Phosphorylation regulates Myc expression via prolonged activation of the mitogen-activated protein kinase pathway. J Cell Physiol 208:133-40
46. Wu WS, Wang FS, Yang KD, Huang CC, Kuo YR (2006) Dexamethasone induction of keloid regression through effective suppression of VEGF expression and keloid fibroblast proliferation. J Invest Dermatol 126:1264-71
47. Wu Y, Zhang Q, Ann DK, Akhondzadeh A, Duong HS, Messadi DV et al. (2004) Increased vascular endothelial growth factor may account for elevated level of plasminogen activator inhibitor-1 via activating ERK1/2 in keloid fibroblasts. Am J Physiol Cell Physiol 286:C905-12
48. Xia W, Longaker MT, Yang GP (2006) P38 MAP kinase mediates transforming growth factor-beta2 transcription in human keloid fibroblasts. Am J Physiol Regul Integr Comp Physiol 290:R501-8
49. Yamamoto M, Tsukada S, Inoue M (1982) Possible age-associated change at cellular level in cultured fibroblasts derived from scar tissue. Chirurgia Plastica 7:51-8
50. You S, Moon JH, Kim TK, Kim SC, Kim JW, Yoon DH et al. (2004) Cellular characteristics of primary and immortal canine embryonic fibroblast cells. Exp Mol Med 36:325-35
51. Zhang Q, Wu Y, Chau CH, Ann DK, Bertolami CN, Le AD (2004) Crosstalk of hypoxia-mediated signaling pathways in upregulating plasminogen activator inhibitor-1 expression in keloid fibroblasts. J Cell Physiol 199:89-97
52. Zhu BH, Zhan WH, Li ZR, Wang Z, He YL, Peng JS et al. (2007) (-)-Epigallocatechin-3-gallate inhibits growth of gastric cancer by reducing VEGF production and angiogenesis. World J Gastroenterol 13:1162-9