Large-scale plasmid DNA processing: Evidence that cell harvesting and storage methods affect yield of supercoiled plasmid DNA

Biotechnology and Applied Biochemistry

Volumn 51, Issue 1, 2008, Pages 43-51

  Kong, Simyee ^a   Rock, Cassandra F ^a,c   Booth, Andrew ^a,d   Willoughby, Nicholas ^a,e   O'Kennedy, Ronan D ^b   Relton, Julian ^b   Ward, John M ^a   Hoare, Mike ^a   Levy, M Susana ^a,f  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

^c Mergers and Acquisitions Group   (United Kingdom)

^d Stephenson Building   (United Kingdom)

^e HERIOT WATT UNIVERSITY   (United Kingdom)

^f Biogenesis Bago   (Argentina)

Author keywords

Cell lysis; Disc stack and solid bowl centrifuges; DNA contamination; Large scale processing; Low temperature storage; Supercoiled plasmid DNA (SC pDNA)

Indexed keywords

CENTRIFUGATION; CENTRIFUGES; CHEMICAL REACTORS; DATA STORAGE EQUIPMENT; DISCHARGE (FLUID MECHANICS); DNA; ESCHERICHIA COLI; GENES; NUCLEIC ACIDS; ORGANIC ACIDS; PILOT PLANTS; SEDIMENTATION; STAGES;

CELL LYSIS; DISC-STACK AND SOLID-BOWL CENTRIFUGES; DNA CONTAMINATION; LARGE-SCALE PROCESSING; LOW-TEMPERATURE STORAGE; SUPERCOILED PLASMID DNA (SC PDNA);

CYTOLOGY;

PLASMID DNA;

ARTICLE; BACTERIAL CELL; BIOREACTOR; CELL SELECTION; CELL STRESS; CELL SUSPENSION; CENTRIFUGATION; CYTOLYSIS; DNA DEGRADATION; DNA SUPERCOILING; ESCHERICHIA COLI; FREEZE THAWING; NONHUMAN; STORAGE;

CELL SEPARATION; CENTRIFUGATION; DNA DAMAGE; DNA, SUPERHELICAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FERMENTATION; FREEZING; MOLECULAR WEIGHT; PLASMIDS; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 51149095172     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20070174     Document Type: Article

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