Purification of essentially RNA free plasmid DNA using a modified Escherichia coli host strain expressing ribonuclease A

Journal of Biotechnology

Volumn 85, Issue 3, 2001, Pages 297-304

  Cooke, G D ^a   Cranenburgh, R M ^b,c   Hanak, J A J ^b   Dunnill, P ^a   Thatcher, D R ^b   Ward, J M ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b KEELE UNIVERSITY   (United Kingdom)

^c UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Chromosomal integration; Gene therapy; Periplasm; RNase

Indexed keywords

DNA; ENZYME KINETICS; ESCHERICHIA COLI; PURIFICATION; RNA;

GENE THERAPY;

GENETIC ENGINEERING;

PLASMID DNA; RIBONUCLEASE A; RNA;

ARTICLE; ENZYME ACTIVITY; ENZYME RELEASE; ENZYME STABILITY; ESCHERICHIA COLI; GENE THERAPY; HYDROLYSIS; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; BIOTECHNOLOGY; CATTLE; CELL DIVISION; DNA, BACTERIAL; DNA, RECOMBINANT; DRUG CONTAMINATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE THERAPY; PLASMIDS; RIBONUCLEASE, PANCREATIC; RNA, BACTERIAL;

ANIMALIA; BOVINAE; ESCHERICHIA COLI;

EID: 0035936936     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(00)00378-3     Document Type: Article

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