Removal of contaminant nucleic acids by nitrocellulose filtration during pharmaceutical-grade plasmid DNA processing

Journal of Biotechnology

Volumn 76, Issue 2-3, 2000, Pages 197-205

  Levy, M S ^a   Collins, I J ^a   Tsai, J T ^a   Ayazi Shamlou, P ^a   Ward, J M ^a   Dunnill, P ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Chromosomal contamination; DNA vaccination; Gene therapy; Nitrocellulose; Plasmid purification

Indexed keywords

BIOREACTORS; CELL MEMBRANES; CHROMATOGRAPHIC ANALYSIS; FILTRATION; GENES; GENETIC ENGINEERING; IMPURITIES; POLYETHYLENE GLYCOLS; PURIFICATION; REMOVAL; SODIUM CHLORIDE; VACCINES;

ANION EXCHANGE CHROMATOGRAPHY; CHROMOSOMAL CONTAMINATION; DNA VACCINATION; DOWNSTREAM PROCESSES; GENE THERAPY; NITROCELLULOSE FILTRATION; PLASMID PURIFICATION;

DNA;

BACTERIAL DNA; DNA VACCINE; MACROGOL; NUCLEIC ACID; PLASMID DNA; PYROXYLIN; RNA; SODIUM CHLORIDE;

AGAR GEL ELECTROPHORESIS; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BIOREACTOR; CELL LYSATE; CHROMOSOME; CONTAMINATION; CONTROLLED STUDY; DNA DETERMINATION; DNA PURIFICATION; DNA SUPERCOILING; ESCHERICHIA COLI; FILTRATION; GENE THERAPY; IONIC STRENGTH; MEMBRANE; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; PURIFICATION; SOUTHERN BLOTTING;

BLOTTING, SOUTHERN; CHROMATOGRAPHY, ION EXCHANGE; CHROMOSOMES, BACTERIAL; COLLODION; DRUG INDUSTRY; ESCHERICHIA COLI; FILTRATION; MEMBRANES, ARTIFICIAL; NUCLEIC ACIDS; PLASMIDS; PRECIPITATION; RNA, BACTERIAL; SODIUM CHLORIDE;

EID: 0033990180     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00189-3     Document Type: Article

References (23)

1. Birnboim H.C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1979;1513-1523.
2. Blanco C., Ritzenthaler P., Mata-Gilsinger M. Nucleotide sequence of a regulatory region of the uidA gene in Escherichia coli K12. Mol. Gen. Genet. 199:1985;101-105.
3. Carlson A., Signs M., Lierman L., Boor R., Jim Jem K. Mechanical disruption of Escherichia coli for plasmid recovery. Biotechnol. Bioeng. 48:1995;303-315.
4. Cohen S.N., Miller C.A. Multiple molecular species of circular R-factor DNA isolated from E. coli. Nature. 224:1969;1273-1277.
5. Durland R.H., Eastman E.M. Manufacturing and quality control of plasmid-based gene expression systems. Adv. Drug Deliv. Rev. 30:1998;33-48.
6. Holmes D.S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114:1981;193-197.
7. Horn N.A., Meek J.A., Budahazi G., Marquet M. Cancer gene therapy using plasmid DNA: purification of DNA for human clinical trials. Hum. Gene Ther. 6:1995;565-573.
8. Lahijani R., Duhon M., Lusby E., Betita H., Marquet M. Quantitation of host cell DNA contaminate in pharmaceutical-grade plasmid DNA using competitive polymerase chain reaction and enzyme-linked immunosorbent assay. Hum. Gene Ther. 9:1998;1181-1186.
9. Levy M.S., Collins I.J., Yim S.S., Ward J.M., Titchener-Hooker N., Ayazi Shamlou P., Dunnill P. Effect of shear on plasmid DNA in solution. Bioprocess Eng. 20:1999;7-13.
10. Levy M.S., Ciccolini L.A.S., Yim S.S., Tsai J.T., Titchener-Hooker N., Ayazi Shamlou P., Dunnill P. The effects of material properties and fluid flow intensity on plasmid DNA recovery during cell lysis. Chem. Eng. Sci. 54:1999;3171-3178.
11. Leyva A.J.R., Kelly W.N. Measurement of DNA in cultured human cells. Anal. Biochem. 62:1974;173-179.
12. Liu Y., Bai J., Liang X., Lubman D.M. Use of a nitrocellulose film substrate in matrix-assisted laser desorption/ionization mass spectrometry for DNA mapping and screening. Anal. Chem. 67:1995;3482-3490.
13. Lui M., Tempst P., Erdjument-Bromage H. Methodological analysis of protein-nitrocellulose interactions to design a refined digestion protocol. Anal. Biochem. 241:1996;156-166.
14. Marquet M., Horn N.A., Meek J.A. Characterisation of plasmid DNA vectors for use in human gene therapy 2. BioPharm. 10:1997;40-45.
15. Middaugh C.R., Evans R.K., Montgomery D.L., Casimiro D.R. Analysis of plasmid DNA from a pharmaceutical perspective. J. Pharm. Sci. 87:1998;131-146.
16. Popovic D.A., Wintzerith W. Hydrophobic interaction of ribonucleic acids on a nitrocellulose column. J. Chromatogr. 268:1983;285-290.
17. Prazeres D., Schulep T., Cooney C. Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography. J. Chromatogr. 806:1998;31-45.
18. Reinikainen P., Korpela K., Nissinen V., Oikku J., Soderlund H., Markkanen P. Escherichia coli plasmid production in fermenter. Biotechnol. Bioeng. 33:1989;386-393.
19. Rush M.G., Warner R. Alkali denaturation of covalently closed circular duplex deoxyribonucleic acid. J. Biol. Chem. 245:1970;2704-2708.
20. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning - A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, New York.
21. Theodossiou I., Collins I.J., Ward J.M., Thomas O.R.T., Dunnill P. The processing of a plasmid-based gene from E. coli. Primary recovery by filtration. Bioprocess Eng. 16:1997;175-183.
22. Theodossiou I., Thomas O.R.T., Dunnill P. Methods of enhancing the recovery of plasmid genes from neutralised cell lysate. Bioprocess Eng. 20:1999;147-156.
23. Wohlhieter J.A., Falkow S., Citarella R.V. Purification of episomal DNA with cellulose nitrate membrane filters. Biochim. Biophys. Acta. 129:1966;475-481.