PCR-mediated one-step deletion of targeted chromosomal regions in haploid Saccharomyces cerevisiae

Applied Microbiology and Biotechnology

Volumn 80, Issue 3, 2008, Pages 545-553

  Sugiyama, Minetaka ^a   Nakazawa, Toshimasa ^a   Murakami, Kiriko ^c   Sumiya, Takahiro ^b   Nakamura, Atsushi ^b   Kaneko, Yoshinobu ^a   Nishizawa, Masafumi ^c   Harashima, Satoshi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

^c KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMOSOMES; COSMIC RAYS; INTEGRAL EQUATIONS; STRAIN;

CHROMOSOMAL DELETIONS; CHROMOSOMAL REGIONS; GENE DELETION; GROWTH DEFECTS; HIGH-EFFICIENCY; NORMAL GROWTH; PHENOTYPIC ANALYSES; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; YEAST GROWTH;

GENES;

CHROMOSOME; GENE EXPRESSION; GENETIC ENGINEERING; GENOME; GROWTH RESPONSE; POLYMERASE CHAIN REACTION; YEAST;

ARTICLE; CHROMOSOME 13; CHROMOSOME 15; CHROMOSOME DELETION; FUNGUS GROWTH; GENE; GROWTH DISORDER; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

CHROMOSOME DELETION; CHROMOSOMES, FUNGAL; HAPLOIDY; OPEN READING FRAMES; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 50249114055     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1609-9     Document Type: Article

References (26)

1. Amberg DC, Burke DJ, Strathern JN (2005) Methods in yeast genetics. Cold Harbor Spring Laboratory, Cold Spring Harbor, New York
2. CB Brachmann A Davies GJ Cost E Caputo J Li P Hieter JD Boeke 1998 Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications Yeast 14 115 132
3. KA Datsenko BL Wanner 2000 One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products Proc Natl Acad Sci USA 97 6640 6645
4. N Dean 1995 Yeast glycosylation mutants are sensitive to aminoglycosides Proc Natl Acad Sci USA 92 1287 1291
5. G Giaever AM Chu L Ni C Connelly L Riles S Veronneau S Dow A Lucau-Danila K Anderson B Andre AP Arkin A Astromoff ME Bakkoury R Bangham R Benito S Brachat S Campanaro M Curtiss K Davis A Deutschbauer KD Entian P Flaherty F Foury DJ Garfinkel M Gerstein D Gotte U Guldener JH Hegemann S Hempel Z Herman DF Jaramillo DE Kelly SL Kelly P Kotter D LaBonte DC Lamb N Lan H Liang H Liao L Liu C Luo M Lussier R Mao P Menard SL Ooi JL Revuelta CJ Roberts M Rose P Ross-Macdonald B Scherens G Schimmack B Shafer DD Shoemaker S Sookhai-Mahadeo RK Storms JN Strathern G Valle M Voet G Volckaert CY Wang TR Ward J Wilhelmy EA Winzeler Y Yang G Yen E Youngman K Yu H Bussey JD Boeke M Snyder P Philippsen RW Davis M Johnston 2002 Functional profiling of the Saccharomyces cerevisiae genome Nature 418 387 391
6. RD Gietz RH Schiestl 1995 Transforming yeast with DNA Methods Mol Cell Biol 5 255 269
7. Y Giga-Hama H Tohda K Takegawa H Kumagai 2007 Schizosaccharomyces pombe minimum genome factory Biotechnol Appl Biochem 46 147 155
8. M Gong YS Rong 2003 Targeting multi-cellular organisms Curr Opin Genet Dev 13 215 220
9. U Gueldener S Heck T Fiedler J Beinhauer JH Hegemann 1996 A new efficient gene disruption cassete for repeated use in budding yeast Nucleic Acids Res 24 2519 2524
10. Y Hirano K Sugimoto 2007 Cdc13 telomere capping decreases mec1 association but does not affect tel1 association with DNA ends Mol Biol Cell 18 2026 2036
11. K Hirashima T Iwaki K Takegawa Y Giga-Hama H Tohda 2006 A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast Nucleic Acids Res 34 e11
12. H Kawasaki K Ouchi 1994 A DNA construct useful for specific chromosome loss in Saccharomyces cerevisiae J Ferment Bioeng 77 125 130
13. V Kolisnychenko G Plunkett III CD Herring T Feher J Posfai FR Blattner G Posfai 2002 Engineering a reduced Escherichia coli genome Genome Res 12 640 647
14. A Kumar PM Harrison KH Cheung N Lan N Echols P Bertone P Miller MB Gerstein M Snyder 2002 An integrated approach for finding overlooked genes in yeast Nat Biotechnol 20 58 63
15. K Murakami E Tao Y Ito M Sugiyama Y Kaneko S Harashima T Sumiya A Nakamura M Nishizawa 2006 Large scale deletions in the Saccharomyces cerevisiae genome create strains with altered regulation of carbon metabolism Appl Microbiol Biotechnol 75 589 597
16. AW Murray TB Claus JW Szostak 1988 Characterization of two telomeric DNA processing reactions in Saccharomyces cerevisiae Mol Cell Biol 8 4642 4650
17. E Pennock K Buckley V Lundblad 2001 Cdc13 delivers separate complexes to the telomere for end protection and replication Cell 104 387 396
18. S Silberstein G Schlenstedt PA Silver R Gilmore 1998 A role for the DnaJ homologue Scj1p in protein folding in the yeast endoplasmic reticulum J Cell Biol 143 921 933
19. H Su X Wang A Bradley 2000 Nested chromosomal deletions induced with retroviral vectors in mice Nature 24 92 95
20. M Sugiyama S Ikushima T Nakazawa Y Kaneko S Harashima 2005 PCR-mediated repeated chromosome splitting in Saccharomyces cerevisiae BioTechniques 38 909 914
21. V Taneja S Paul K Ganesan 2004 Directional ligation of long-flanking homology regions to selection cassettes for efficient targeted gene-disruption in Candida albicans FEMS Yeast Research 4 841 847
22. LR Vega MK Mateyak VA Zakian 2003 Getting to the end: telomerase access in yeast and humans Nat Rev Mol Cell Biol 4 948 959
23. A Wach A Brachat R Pohlmann P Philippsen 1994 New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae Yeast 10 1793 1808
24. F Winston C Dollard S Ricupero-Hovasse 1995 Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C Yeast 11 53 55
25. K Yamagishi M Sugiyama Y Kaneko S Harashima 2008 Conditional chromosome splitting in Saccharomyces cerevisiae using the homing endonuclease PI-SceI Appl Microbiol Biotechnol 79 699 706
26. BJ Yu BH Sung MD Koob CH Lee JH Lee WS Lee MS Kim SC Kim 2002 Minimization of the Escherichia coli genemo using a Tn5-targeted Cre/loxP excision system Nat Biotechnol 20 1018 1023