Conditional chromosome splitting in Saccharomyces cerevisiae using the homing endonuclease PI-SceI

Applied Microbiology and Biotechnology

Volumn 79, Issue 4, 2008, Pages 699-706

  Yamagishi, Kazuo ^a   Sugiyama, Minetaka ^a   Kaneko, Yoshinobu ^a   Harashima, Satoshi ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

Conditional splitting; Genome reconstruction; PCS technology; PI SceI; Single gene chromosome; Yeast

Indexed keywords

CELL CULTURE; ENZYME KINETICS; GENE ENCODING; GENE EXPRESSION; YEAST;

CONDITIONAL SPLITTING; GENOME RECONSTRUCTION; PCS TECHNOLOGY; PI-SCEI; SINGLE-GENE CHROMOSOMES;

CHROMOSOMES;

ENDONUCLEASE; ENDONUCLEASE PI SCEI;

BIOTECHNOLOGY; CHROMOSOME; GENE; GENOME; YEAST;

ARTICLE; BACTERIAL GENOME; BIOTECHNOLOGY; CHROMOSOME LOSS; CHROMOSOME REPLICATION; CHROMOSOME SPLITTING; CHROMOSOME VARIANT; CHROMOSOMES AND CHROMOSOMAL PHENOMENA; DOWNSTREAM PROCESSING; GENE; GENE EXPRESSION; GENE INDUCTION; GENE LOCUS; GENE SEQUENCE; GENOME; GLC7 GENE; HAPLOIDY; MINICHROMOSOME; MOLECULAR CLONING; MOLECULAR RECOGNITION; NONHUMAN; POLYMERASE CHAIN REACTION; PROMOTER REGION; SACCHAROMYCES CEREVISIAE;

BASE PAIRING; BASE SEQUENCE; CHROMOSOMES, FUNGAL; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA PRIMERS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; REPRESSOR PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TELOMERE; TRANSFORMATION, GENETIC; UBIQUITIN-PROTEIN LIGASE COMPLEXES;

SACCHAROMYCES CEREVISIAE;

EID: 44649136709     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1465-7     Document Type: Article

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