PCR-mediated repeated chromosome splitting in Saccharomyces cerevisiae

BioTechniques

Volumn 38, Issue 6, 2005, Pages 909-914

  Sugiyama, Minetaka ^b   Ikushima, Shigehito ^b   Nakazawa, Toshimasa ^b   Kaneko, Yoshinobu ^b   Harashima, Satoshi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGY; YEAST;

CHROMOSOME ENGINEERING; MITOTIC GROWTH;

GENES;

ARTICLE; CELL GROWTH; CELL TRANSFORMATION; CHROMOSOME 1; CHROMOSOME 15; CHROMOSOME SEGREGATION; HAPLOIDY; MITOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; CHROMOSOME; GENETICS; GENOME; METHODOLOGY; PLASMID; PULSED FIELD GEL ELECTROPHORESIS; SOUTHERN BLOTTING;

SACCHAROMYCES CEREVISIAE;

BLOTTING, SOUTHERN; CHROMOSOMES, FUNGAL; ELECTROPHORESIS, GEL, PULSED-FIELD; GENOME, FUNGAL; PLASMIDS; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

EID: 27744449273     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05386RR01     Document Type: Article

References (23)

1. Kolisnychenko, V., G. Plunkett III, C.D. Herring, T. Fehér, J. Pósfai, F.R. Blattner, and G. Pósfai. 2002. Engineering a reduced Escherichia coli genome. Genome Res. 12:640-647.
2. Yu, B.J., B.H. Sung, M.D. Koob, C.H. Lee, J.H. Lee, W.S. Lee, M.S. Kim, and S.C. Kim. 2002. Minimization of the Escherichia coli genome using a Tn5-targeted Cre/loxP excision system. Nat. Biotechnol. 20:1018-1023.
3. Delneri, D., I. Colson, S. Grammenoudi, I.N. Roberts, E.J. Louis, and S.G. Oliver. 2003. Engineering evolution to study speciation in yeasts. Nature 422:68-72.
4. Yang, J.W., C. Pendon, J. Yang, N. Haywood, A. Chand, and W.R. Brown. 2000. Human mini-chromosomes with minimal centromeres. Hum. Mol. Genet. 9:1891-1902.
5. Westers, H., R. Dorenbos, J.M. van Dijl, J. Kabel, T. Flanagan, K.M. Devine, F. Jude, S.J. Seror, et al. 2003. Genome engineering reveals large dispensable regions in Bacillus subtilis. Mol. Biol. Evol. 20:2076-2090.
6. Widianto, D., Y. Mukai, K.-H. Kim, S. Harashima, and Y. Oshima. 1996. One-step splitting of a chromosome in haploid cells of Saccharomyces cerevisiae and its effect on the cell proliferation. J. Ferment. Bioeng. 82:199-204.
7. Widianto, D., E. Yamamoto, M. Sugiyama, Y. Mukai, Y. Kaneko, Y. Oshima, M. Nishizawa, and S. Harashima. 2003. Creating a Saccharomyces cerevisiae haploid strain having 21 chromosomes. J. Biosci. Bioeng. 95:89-94.
8. Vollrath, D., R.W. Davis, C. Connelly, and P. Hieter. 1988. Physical mapping of large DNA by chromosome fragmentation. Proc. Natl. Acad. Sci. USA 85:6027-6031.
9. Widianto, D., E. Yamamoto, Y. Mukai, Y. Oshima, and S. Harashima. 1997. A method for fusing chromosomes in Saccharomyces cerevisiae. J. Ferment. Bioeng. 83:125-131.
10. Mizukami, A., E. Nagamori, Y. Takakura, S. Matsunaga, Y. Kaneko, S. Kajiyama, S. Harashima, A. Kobayashi, et al. 2003. Transformation of yeast using calcium alginate microbeads with surface-immobilized chromosomal DNA. BioTechniques 35:738-740.
11. Winston, F., C. Dollard, and S.L. Ricupero-Hovasse. 1995. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11:53-55.
12. Kitada, K., E. Yamaguchi, and M. Arisawa. 1995. Cloning of the Candida glabrata TRP 1 and HIS3 genes, and construction of their disruptant strains by sequential integrative transformation. Gene 165:203-206.
13. Güldener, U., S. Heck, T. Fiedler, J. Beinhauer, and J.H. Hegemann. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res. 24:2519-2524.
14. Gietz, R.D. and R.H. Schiestl. 1995. Transforming yeast with DNA. Methods Mol. Cell. Biol. 5:255-269.
15. Sheenan, C. and A.S. Weiss. 1990. Yeast artificial chromosomes: rapid extraction for high resolution analysis. Nucleic Acids Res. 18:2193
16. Szostak, J.W. and E.H. Blackburn. 1982. Cloning yeast telomeres on linear plasmid vectors. Cell 29:245-255.
17. Murray, A.W., T.B. Claus, and J.W. Szostak. 1988. Characterization of two telomeric DNA processing reactions in Saccharomyces cerevisiae. Mol. Cell. Biol. 8:4642-4650.
18. Wyrick, J.J., J.G. Aparicio, T. Chen, J.D. Barnett, E.G. Jennings, R.A. Young, S.P. Bell, and O.M. Aparicio. 2001. Genome-wide distribution of ORC and MCM proteins in S. cerevisiae: high-resolution mapping of replication origins. Science 294:2357-2360.
19. Ho, S.N., H.D. Hunt, R.M. Horton, J.K. Pullen, and L.R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59.
20. Dillon, P.J. and C.A. Rosen. 1990. A rapid method for the construction of synthetic genes using the polymerase chain reaction. BioTechniques 9:298-299.
21. Hieter, P., C. Mann, M. Snyder, and R.W. Davis. 1985. Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss. Cell 40:381-392.
22. Surosky, R.T., C.S. Newlon, and B.-K. Tye. 1986. The mitotic stability of deletion derivatives of chromosome III in yeast. Proc. Natl. Acad. Sci. USA 83:414-418.
23. Murray, A.W., N.P. Schultes, and J.W. Szostak. 1986. Chromosome length controls mitotic chromosome segregation in yeast. Cell 45:529-536.