Improving the yield of soluble 6xHis-tagged interferon-α via the addition of repressor of the araBAD promoter system in Escherichia coli

Biotechnology Letters

Volumn 30, Issue 9, 2008, Pages 1577-1582

  Jung, Kyung Hwan ^a   Park, Young Seo ^b   Yeon, Ji Hyeon ^a   Kim, Sung Hun ^a   Yoo, Sun Kyun ^c   Jang, Byeong Churl ^d  

^a Chungju National University   (South Korea)

^b Gachon University College of Medicine   (South Korea)

^c Joongbu University   (South Korea)

^d Keimyung University   (South Korea)

Author keywords

D glucopyranoside; araBAD promoter; D fucose; Escherichia coli; Interferon

Indexed keywords

CHELATION; COLLOIDS; ESCHERICHIA COLI; GELATION; GELS; GLYCOPROTEINS; NICKEL; NICKEL ALLOYS; PURIFICATION;

AGAROSE GELS; ARABAD PROMOTER; D-FUCOSE; DRY CELL WEIGHT; GLUCOPYRANOSIDE; INCLUSION BODIES;

ANTIBIOTICS;

ALPHA INTERFERON; GLYCEROL; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; OLIGOPEPTIDE; RECOMBINANT PROTEIN; REPRESSOR PROTEIN;

AGAR GEL ELECTROPHORESIS; ARTICLE; CELL INCLUSION; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; OPERON; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; SOLUBILITY; WESTERN BLOTTING;

BLOTTING, WESTERN; ELECTROPHORESIS, AGAR GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GLYCEROL; HISTIDINE; HUMANS; INCLUSION BODIES; INTERFERON-ALPHA; OLIGOPEPTIDES; OPERON; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; REPRESSOR PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 49749137510     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9741-2     Document Type: Article

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