Solubility and proteolysis of the Zb-MalE and Zb-MalE31 proteins during overproduction in Escherichia coli

Biotechnology and Bioengineering

Volumn 90, Issue 2, 2005, Pages 239-247

  Sandén, Anna Maria ^a   Boström, Maria ^a   Markland, Katrin ^a   Larsson, Gen ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

Escherichia coli; Fed batch; Feed rate; Inclusion bodies; Proteolysis; Solubility; Zb MalE; Zb MalE31

Indexed keywords

AMINO ACIDS; ESCHERICHIA COLI; MALTOSE; MOLECULAR STRUCTURE; POLYPEPTIDES;

FEED RATE; ISOFORMS; POLYPEPTIDE CONTROLS; PROTEOLYSIS;

PROTEINS;

AMINO ACID; BACTERIAL PROTEIN; HYBRID PROTEIN; MALE PROTEIN; MALE31 PROTEIN; MALTOSE BINDING PROTEIN; POLYPEPTIDE; UNCLASSIFIED DRUG; ZB MALE PROTEIN; ZB MALE31 PROTEIN;

ARTICLE; BACTERIUM CULTURE; CELL INCLUSION; ESCHERICHIA COLI; FED BATCH REACTOR; FEED RATE; HYPOTHESIS; NONHUMAN; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SOLUBILITY; TRANSCRIPTION REGULATION;

BLOTTING, WESTERN; CARRIER PROTEINS; CULTURE MEDIA; ESCHERICHIA COLI; HYDROLYSIS; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT PROTEINS; SOLUBILITY;

EID: 17744363459     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20433     Document Type: Article

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