Methyl α-D-glucopyranoside enhances the enzymatic activity of recombinant β-galactosidase inclusion bodies in the araBAD promoter system of Escherichia coli

Journal of Industrial Microbiology and Biotechnology

Volumn 35, Issue 7, 2008, Pages 695-701

  Jung, Kyung Hwan ^a   Yeon, Ji Hyeon ^a   Moon, Sung Kwon ^a   Choi, Joon Ho ^b  

^a Chungju National University   (South Korea)

^b Korea Hemophilia Foundation Clinic   (South Korea)

Author keywords

Galactosidase inclusion body; araBAD promoter system; Escherichia coli; Methyl D glucopyranoside; Protein expression rate

Indexed keywords

BETA GALACTOSIDASE; ESCHERICHIA COLI PROTEIN; GLUCOPYRANOSIDE; GUANIDINE; METHYL ALPHA DEXTRO GLUCOPYRANOSIDE; PROTEIN ARABAD; RECOMBINANT ENZYME;

ARTICLE; BACTERIUM CULTURE; BIOREACTOR; CELL INCLUSION; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; MICROTITER PLATE ASSAY; NONHUMAN; PROMOTER REGION; PROTEIN AGGREGATION; SOLUBILIZATION; STRUCTURAL GENE; TRANSCRIPTION REGULATION;

BETA-GALACTOSIDASE; DOWN-REGULATION; ENZYME ACTIVATORS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; INCLUSION BODIES; METHYLGLUCOSIDES; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 46949107249     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-008-0329-6     Document Type: Article

References (27)

1. Barríos-Rivera SJ, Yang Y-T, Bennett GN, San K-Y (2000) Effect of glucose analog spplementation on metabolic flux distribution in ananerobic chemostat cultures of Escherichia coli. Metabolic Eng 2:149-154
2. Capelle MAH, Gurny R, Arvinte T (2007) High throughput screening of protein formulation stability: practical considerations. Eur J Pharm Biopharm 65:131-148
3. Carrió M, González-Montalbán N, Vera A, Villaverde A, Ventura S (2005) Amyloid-like properties of bacterial inclusion bodies. J Mol Biol 347:1025-1037
4. Chou C-H, Bennett GN, San K-Y (1994) Effect of modulated glucose uptake on high-level recombinant protein production in a dense Escherichia coli culture. Biotechnol Prog 10:644-647
5. de Groot NS, Ventura S (2006) Effect of temperature on protein quality in bacterial inclusion bodies. FEBS Lett 580:6471-6476
6. de Groot NS, Ventura S (2006) Protein activity in bacterial inclusion bodies correlates with predicted aggregation rates. J Biotechnol 125:110-113
7. Duetz WA, Rüedi L, Hermann R, O'Connor K, Büchs J, Witholt B (2000) Methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates. Appl Environ Micorbiol 66:2641-2646
8. Garcia-Fruitos E, Carrio MM, Aris A, Villaverde A (2005) Folding of a misfolding-prone β-galactosidase in absence of DnaK. Biotechnol Bioeng 90:869-875
9. García-Fruitós E, González-Montalbán N, Morell M, Vera A, Ferraz RM, Arís A, Ventura S, Villaverde A (2005) Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins. Microb Cell Fact 4:27
10. García-Fruitós E, Martínez-Alonso M, Gonzàlez-Montalbán N, Valli M, Mattanovich D, Villaverde A (2007) Divergent genetic control of protein solubility and conformational quality in Escherichia coli. J Mol Biol 374:195-205
11. González-Montalbán N, García-Fruitós E, Villaverde A (2007) Recombinant protein solubility-does more mean better? Nature Biotechnol 718-720
12. Jevsevar S, Gaberc-Porekar V, Fonda I, Podobnik B, Grdadolnik J, Menart V (2005) Production of nonclassical inclusion bodies from which correctly folded protein can be extracted. Biotechnol Prog 21:632-639
13. Jung K-H (2008) Enhanced enzyme activities of inclusion bodies of recombinant β-galactosidase via the addition of inducer analog after l-arabinose induction in the araBAD promoter system of Escherichia coli. J Microbiol Biotechnol (in press)
14. Lee Y-J, Jung K-H (2007) Modulation of the tendency towards inclusion body formation of recombinant protein by the addition of glucose in the araBAD promoter system of Escherichia coli. J Microbiol Biotechnol 17:1898-1903
15. Lichenstein HS, Hamilton EP, Lee N (1987) Repression and catabolite gene activation in the araBAD operon. J Bacteriol 169:811-822
16. Martínez-Alonso M, Vera A, Villaverde A (2007) Role of the chaperone DnaK in protein solubility and conformational quality in inclusion body-forming Escherichia coli cells. FEMS Microbiol Lett 273:187-195
17. Miller JM (1973) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, New York
18. Miller JM (2001) Time course assay of β-galactosidase. In: Hardin C, Edwards J, Riell A, Presutti D, Miller W, Robertson D (eds) Cloning, gene expression, and protein purification. Experimental procedures and process rationale. Oxford University Press, Oxford, pp 292-293
19. Schleif R (2000) Regulation of the l-arabinose operon of Escherichia coli. Trends Genet 16:559-565
20. Smith AM, Chan J, Oksenberg D, Urfer R, Wexler DS, Ow A, Gao L, McAlorum A, Huang SG (2004) A high-throughput turbidometric assay for screening inhibitors of protein disulfide isomerase activity. J Biomol Screen 9:614-620
21. Sørensen HP, Mortensen KK (2005) Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 115:113-128
22. Tokatlidis K, Dhurjati P, Millet J, Béguin P, Aubert JP (1991) High activity of inclusion bodies formed in Escherichia coli overproducing Clostridium thermocellum endoglucanase D. FEBS Lett 282:205-208
23. van den Berg B, Ellis RJ, Dobson CM (1999) Effects of macromolecular crowding on protein folding and aggregation. EMBO J 18:6927-6933
24. Ventura S, Villaverde A (2006) Protein quality in bacterial inclusion bodies. Trends Biotechnol 24:179-185
25. Vera A, González-Montalbán N, Arís A, Villaverde A (2007) The conformational quality of insoluble recombinant proteins is enhanced at low growth temperatures. Biotechnol Bioeng 96:1101-1106
26. Worrall DM, Goss NH (1989) The formation of biologically active β-galactosidase inclusion bodies in Escherichia coli. Aust J Biotechnol 3:28-32
27. Zhang X, Schleif R (1989) Catabolite gene activator protein mutations affecting activity of the araBAD promoter. J Bacteriol 180:195-200