Catabolite gene activator protein mutations affecting activity of the araBAD promoter

Journal of Bacteriology

Volumn 180, Issue 2, 1998, Pages 195-200

  Zhang, Xin ^a,b   Schleif, Robert ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; DNA FRAGMENT; MUTANT PROTEIN; RNA POLYMERASE;

ARTICLE; ESCHERICHIA COLI; GENE ACTIVATION; GENE EXPRESSION REGULATION; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PROTEIN INTERACTION; TRANSCRIPTION REGULATION;

EID: 0031909984     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.2.195-200.1998     Document Type: Article

References (42)

1. Aiba, H., T. Nakamura, H. Mitani, and H. Mori. 1985. Mutations that alter the allosteric nature of cAMP receptor protein of Escherichia coli. EMBO J. 4:3329-3332.
2. Bell, A., K. Gaston, R. Williams, K. Chapman, A. Kolb, H. Bue, S. Minchin, J. Williams, and S. Busby. 1990. Mutations that alter the ability of the Escherichia coli cyclic AMP receptor protein to activate transcription. Nucleic Acids Res. 18:7243-7250.
3. Blatter, E. E., W. Ross, H. Tang, R. L. Gourse, and R. H. Ebright. 1994. Domain organization of RNA polymerase alpha subunit: C-terminal 85 amino acids constitute a domain capable of dimerization and DNA binding. Cell 78:889-896.
4. Bracco, L., D. Kotlarz, A. Kolb, S. Diekmann, and H. Buc. 1989. Synthetic curved DNA sequences can act as transcriptional activators in Escherichia coli. EMBO J. 8:4289-4296.
5. Busby, S., and R. Ebright. 1997. Transcription activation at class II CAPdependent promoters. Mol. Microbiol. 23:853-859.
6. Busby, S., D. West, M. Lawes, C. Webster, A. Ishihama, and A. Kolb. 1994. Transcription activation by the Escherichia coli cyclic AMP receptor protein. J. Mol. Biol. 241:341-352.
7. Bustos, S. A., and R. F. Schleif. 1993. Functional domains of the AraC protein. Proc. Natl. Acad. Sci. USA 90:5638-5642.
8. Carra, J. H., and R. F. Schleif. 1993. Variation of half-site organization and DNA looping by AraC protein. EMBO J. 12:35-14.
9. Dunn, T. M., S. Hahn, S. Ogden, and R. F. Schleif. 1984. An operator at -280 base pairs that is required for repression of araBAD operon promoter: addition of DNA helical turns between the operator and promoter cyclically hinders repression. Proc. Natl. Acad. Sci. USA 81:5017-5020.
10. Ebright, R. H. 1993. Transcription activation at class I CAP-dependent promoters. Mol. Microbiol. 8:797-802.
11. Egan, S. M., and R. F. Schleif. 1993. A regulatory cascade in the induction of rhaBAD. J. Mol. Biol. 234:87-98.
12. Eschenlauer, A. C., and W. S. Reznikoff. 1991. Escherichia coli catabolite gene activator protein mutants defective in positive control of lac operon transcription. J Bacteriol. 173:5024-5029.
13. Greenblatt, J., and R. Schleif. 1971. Arabinose C protein: regulation of the arabinose operon in vitro. Nat. New Biol. 233:166-170.
14. Hahn, S., T. Dunn, and R. Schleif. 1984. Upstream repression and CRP stimulation of the Escherichia coli L-arabinose operon. J. Mol. Biol. 180:61-72.
15. 2 site. J. Mol. Biol. 188:355-367.
16. Hendrickson, W., and R. F. Schleif. 1984. Regulation of the Escherichia coli L-arabinose operon studied by gel electrophoresis DNA binding assay. J. Mol. Biol. 178:611-628.
17. Hendrickson, W., C. Stoner, and R. Schleif. 1990. Characterization of the Escherichia coli araFGH and araJ promoters. J. Mol. Biol. 215:497-510.
18. Hu, J. C, and C. A. Gross. 1985. Mutations in the sigma subunit of E. coli RNA polymerase which affect positive control of transcription. Mol. Gen. Genet. 199:7-13.
19. Igarashi, K., and A. Ishihama. 1991. Bipartite functional map of the E. coli RNA polymerase alpha subunit: involvement of the C-terminal region in transcription activation by cAMP-CRP. Cell 65:1015-1022.
20. Jeon, Y., T. Yamazaki, T. Otomo, A. Ishihama, and Y. Kyogoku. 1997. Flexible linker in the RNA polymerase alpha subunit facilitates the independent motion of the C-terminal activator contact domain. J. Mol. Biol. 267:953-962.
21. 70 subunit of E. coli RNA polymerase. J. Biol. Chem. 270:19213-19216.
22. Johnson, C. M., and R. F. Schleif. 1995. In vivo induction kinetics of the arabinose promoters in Escherichia coli. J. Bacteriol. 177:3438-3442.
23. Joung, J. K., L. U. Le, and A. Hochschild. 1993. Synergistic activation of transcription by Escherichia coli cAMP receptor protein. Proc. Natl. Acad. Sci. USA 90:3083-3087.
24. Lee, N., C. Francklyn, and E. P. Hamilton. 1987. Arabinose-induced binding of AraC protein to araI2 activates the anaBAD operon promoter. Proc. Natl. Acad. Sci. USA 84:8814-8818.
25. Lichenstcin, H. S., E. P. Hamilton, and N. Lee. 1987. Repression and catabolite gene activation in the araBAD operon. J. Bacteriol. 169:811-822.
26. Lobell, R. B., and R. F. Schleif. 1990. DNA looping and unlooping by AraC protein. Science 250:528-532.
27. Lobell, R. B., and R. F. Schleif. 1991. AraC-DNA looping: orientation and distance-dependent loop breaking by the cyclic AMP receptor protein. J. Mol. Biol. 218:45-54.
28. Murakami, K., M. Kimura, J. T. Owens, C. F. Meares, and A. Ishihama. 1997. The two alpha subunits of Escherichia coli RNA polymerase are asymmetrically arranged and contact different halves of DNA upstream element. Proc. Natl. Acad. Sci. USA 94:1709-1714.
29. Niu, W., Y. Kim, G. Tau, T. Heyduk, and R. H. Ebright. 1996. Transcription activation at class II CAP-dependent promoters: two interactions between CAP and RNA polymerase. Cell 87:1123-1134.
30. Niu, W., Y. Zhou, Q. Dong, Y. W. Ebright, and R. H. Ebright. 1994. Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP). I. Saturation and alanine-scanning mutagenesis. J. Mol. Biol. 243:595-602.
31. Passner, J., and T. Steitz. 1997. The structure of a CAP-DNA complex having two cAMP molecules bound to each monomer. Proc. Natl. Acad. Sci. USA 94:2843-2847.
32. Reeder, T., and R. Schleif. 1993. AraC protein can activate transcription from only one position and when pointed in only one direction. J. Mol. Biol. 231:205-218.
33. Richet, E., and L. Sögaard-Andersen. 1994. CRP induces the repositioning of MalT at the Escherichia coli malKp promoter primarily through DNA bending. EMBO J. 13:4558-4567.
34. Schleif, R., and P. Wensink. 1981. Practical methods in molecular biology. Springer-Verlag, New York, N. Y.
35. Simons, R. W., F. Houman, and N. Kieckner. 1987. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53:85-96.
36. Stoltzfus, L., and G. Wilcox. 1989. Effect of mutations in the cyclic AMP receptor protein-binding site on araBAD and araC expression. J. Bacteriol. 171:1178-1184.
37. Williams, R., A. Bell, G. Sims, and S. Busby. 1991. The role of two surface exposed loops in transcription activation by the Escherichia coli CRP and FNR proteins. Nucleic Acids Res. 19:6705-6712.
38. 37a.Withey, J., and R. Schleif. Unpublished data.
39. BAD. J. Mol. Biol. 258:14-24.
40. Zhang, X. P., A. Gunasekera, Y. W. Ebright, and R. H. Ebright. 1991. Derivatives of CAP having no solvent-accessible cysteine residues, or having a unique solvent-accessible cysteine residue at amino acid 2 of the helix-turn-helix motif. J. Biomol. Struct. Dyn. 9:463-473.
41. Zhou, Y., X. Zhang, and R. H. Ebright. 1993. Identification of the activating region of catabolite gene activator protein (CAP): isolation and characterization of mutants of CAP specifically defective in transcription activation. Proc. Natl. Acad. Sci. USA 90:6081-6085.
42. Zhou, Y. H., X. P. Zhang, and R. H. Ebright. 1991. Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase. Nucleic Acids Res. 19:6052.