Integrated approach for detection of nonculturable cells of Ralstonia solanacearum in asymptomatic Pelargonium spp. cuttings

Phytopathology

Volumn 98, Issue 8, 2008, Pages 949-955

  Marco Noales, E ^a   Bertolini, E ^a   Morente, C ^a   Lopez M M ^a  

^a INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS IVIA   (Spain)

Author keywords

Latent infection; Survival

Indexed keywords

ARTICLE; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PELARGONIUM; PHYSIOLOGY; PLANT DISEASE; POLYMERASE CHAIN REACTION; RALSTONIA SOLANACEARUM; SENSITIVITY AND SPECIFICITY; TOMATO;

BACTERIOLOGICAL TECHNIQUES; LYCOPERSICON ESCULENTUM; PELARGONIUM; PLANT DISEASES; POLYMERASE CHAIN REACTION; RALSTONIA SOLANACEARUM; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); LYCOPERSICON ESCULENTUM; PELARGONIUM; RALSTONIA SOLANACEARUM;

EID: 49449086037     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO-98-8-0949     Document Type: Article

References (68)

1. Álvarez, A. M. 2004. Integrated approaches for detection of plant pathogenic bacteria and diagnosis of bacterial diseases. Annu. Rev. Phytopathol. 42:339-366.
2. Anonymous. 1998. Council Directive 98/57/EC of 20 July 1998 on the control of Ralstonia solanacearum (Smith) Yabuuchi et al. Off. J. Eur. Communities L235/1:21.08.98.
3. Anonymous. 2003. Ralstonia solanacearum, race 3 (Biovar2) intercepted on imported Pelargonium spp.: Trace back, survey and regulatory action. Phytosanitary Alert System of North American Plant Protection Organization (NAPPO).
4. Anonymous. 2004. Ralstonia solanacearum race 3 biovar 2 in a Florida Nursery. Official Pest Reports. National Plant Protection Organization.
5. APHIS-PPQ. 2004. USDA-APHIS pest alert: Ralstonia solanacearum race 3 biovar 2 Southern wilt of geranium. Published online by USDA-APHIS PPQ Pest Detection and Management Programs.
6. Biosca, E. G., Caruso, P., Bertolini, E., Álvarez, B., Palomo, J. L., Gorris, M. T., and López, M. M. 2005. Improved detection of Ralstonia solanacearum in culturable and VBNC state from water samples at low temperatures. Pages 501-506 in: Bacterial Wilt Disease and the Ralstonia solanacearum Species Complex. C. Allen, P. Prior, and C. Hayward, eds. American Phytopathological Society, St. Paul, MN.
7. Boudazin, G., Le Roux, A. C., Josi, K., Labarre, P., and Jouan, B. 1999. Design of division specific primers of Ralstonia solanacearum and application to the identification of European isolates. Eur. J. Plant Pathol. 105:373-380.
8. Bradbury, J. F. 1986. Guide to Plant Pathogenic Bacteria. CAB International, Wallingford, UK.
9. Caruso, P., Bertolini, E., Cambra, M., and López, M. M. 2003. A new and sensitive co-operational polymerase chain reaction (Co-PCR) for a rapid detection of Ralstonia solanacearum in water. J. Microbiol. Methods 55:257-272.
10. Caruso, P., Gorris, M. T., Cambra, M., Palomo, J. L., Collar, J., and López, M. M. 2002. Enrichment double-antibody sandwich indirect enzyme-linked immunosorbent assay that uses a specific monoclonal antibody for sensitive detection of Ralstonia solanacearum in asymptomatic potato tubers. Appl. Environ. Microbiol. 68:3634-3638.
11. Caruso, P., Palomo, J. L., Bertolini, E., Álvarez, B., López, M. M., and Biosca, E. G. 2005. Seasonal variation of Ralstonia solanacearum biovar 2 populations in a Spanish river: Recovery of stressed cells at low temperatures. Appl. Environ. Microbiol. 71:140-148.
12. Christensen, J., and Linden, K. G. 2003. How particles affect UV light in the UV disinfection of unfiltered drinking water. J. Am. Water Works Assoc. 95:179-189.
13. Dukan, S., Levi, Y., and Touati, D. 1997. Recovery of culturability of an HOCl-stressed population of Escherichia coli after incubation in phosphate buffer: Resuscitation or regrowth. Appl. Environ. Microbiol. 63:4204-4209.
14. Elphinstone, J. G., Hennessey, J., Wilson, J. K., and Stead, D. E. 1996. Sensitivity of different methods for the detection of Ralstonia solanacearum in potato tuber extracts. EPPO Bull. 26:663-678.
15. Elphinstone, J. G., Stanford, H. M., and Stead, D. E. 1998. Detection of Ralstonia solanacearum in potato tubers, Solanum dulcamara and associated irrigation water extracts. Pages 133-139 in: Bacterial Wilt Disease. Molecular and Ecological Aspects. P. Prior, C. Allen, and J. Elphinstone, eds. Springer-Verlag, Heidelberg, Germany.
16. Emerick, R. W., Loge, F. J., Ginn, T, and Darby, J. L. 2000. Modeling the inactivation of particle-associated coliform bacteria. Water Environ. Res. 72:432-438.
17. Fegan, M., Holoway, G., Hayward, A. C., and Timmis, J. 1998. Development of a diagnostic test based on the polymerase chain reaction (PCR) to identify strains of Ralstonia solanacearum exhibiting the biovar 2 genotype. Pages 34-43 in: Bacterial Wilt Disease. Molecular and Ecological Aspects. P. Prior, C. Allen, and J. Elphinstone, eds. Springer Verlag, Heidelberg, Germany.
18. Ford, T. E. 1993. The microbial ecology of water distribution and outfall systems. Pages 455-482 in: Aquatic Microbiology: An Ecological Approach. T. E. Ford, ed. Blackwell Scientific Publishers, London, UK.
19. French, E. R., Gutarra, L., Alley, P., and Elphinstone, J. 1995. Culture media for Pseudomonas solanacearum isolation, identification and maintenance. Fitopatologia 30:126-130.
20. Gauthier, V., Redercher, S., and Block, J. C. 1999. Chlorine inactivation of Sphingomonas cells attached to goethite particles in drinking water. Appl. Environ. Microbiol. 65:355-357.
21. Glick, D. L., Coffey, C. M., and Sulzinski, M. A. 2002. Simultaneous PCR detection of the two major bacterial pathogens of geranium. J. Phytopathol. 150:54-59.
22. Hamrick, D. 2004. Ralstonia strikes American pelargonium young plants again. FloraCulture International March 14:16-18.
23. Hayward, A. C. 1991. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol. 29:65-87.
24. Hayward, A. C. 1994. The hosts of Pseudomonas solanacearum. Pages 9-24 in: Bacterial Wilt: The Disease and Its Causative Agent, Pseudomonas solanacearum. A. C. Hayward and G. L. Hartman, eds. CAB International Mycological Institute, Wallingford, UK.
25. Hayward, A. C., Elphinstone, J. G., Caffier, D., Janse, J., Stefani, E., French, E. R., and Wright, A. J. 1998. Round table on bacterial wilt (brown rot) of potato. Pages 420-430 in: Bacterial Wilt Disease. Molecular and Ecological Aspects. P. Prior, C. Allen, and J. Elphinstone, eds. Springer Verlag, Heidelberg, Germany.
26. Hudelson, B. D. 1999. Southern wilt. University of Wisconsin Garden Facts, May 11, 1999.
27. Janse, J. D. 1988. A detection method for Pseudomonas solanacearum in symptomless potato tubers and some data on its sensitivity and specificity. Bull. OEPP 18:343-351.
28. Janse, J. D. 1996. Potato brown rot in western Europe-history, present occurrence and some remarks on possible origin, epidemiology and control strategies. Bull. OEPP 26:679-695.
29. Janse, J. D., van den Beld, H. E., Elphinstone, J., Simpkins, S., Tjou-Tam-Sin, N. N. A., and van Vaerenbergh, J. 2004. Introduction to Europe of Ralstonia solanacearum biovar 2, race 3 in Pelargonium zonale cuttings. J. Plant Pathol. 86:147-155.
30. Jolis, D., Lam, C., and Pitt, P. 2001. Particle effects on ultraviolet disinfection of coliform bacteria in recycled water. Water Environ. Res. 73:233-236.
31. Kirchhof, G., Baldani, J., Reis, V. M., and Hartmann, A. 1998. Molecular assay to identify Acetobacter diazotrophicus and detect its occurrence in plant tissues. Can. J. Microbiol. 44:12-19.
32. Lambert, C. D. 2002. Agricultural bioterrorism protection act of 2002: Possession, use, and transfer of biological; agents and toxins; interim and final rule. (7 CFR Part 331). Federal Register 67:76908-76938.
33. LeChevallier, M. W., Welch, N. J., and Smith, D. B. 1999. Full-scale studies of factors related to coliform regrowth in drinking water. Appl. Environ. Microbiol. 62:2201-2211.
34. Lelliot, R. A., and Stead, D. E. 1987. Methods for the diagnosis of bacterial diseases of plants. Page 216 in: Methods in Plant Pathology. vol. 2. T. F. Preece, ed. Blackwell Scientific Publications, Oxford, UK.
35. Loge, F. J., Emerick, R. W., Thompson, D. E., Nelson, D. C., and Darby, J. L. 1999. Factors influencing ultraviolet disinfection performance part I: Light penetration to wastewater particles. Water Environ. Res. 71:377-381.
36. López, M. M., Bertolini, E., Olmos, A., Caruso, P., Gorris, M. T., Llop. P., Penyalver, R., and Cambra, M. 2003. Innovative tools for detection of plant pathogenic viruses and bacteria. Int. Microbiol. 6:233-243.
37. Maes, M., Garbeva, P., and Crepel, C. 1996. Identification and sensitive endophytic detection of the fire blight pathogen Erwinia amylovora with 23S ribosomal DNA sequences and the polymerase chain reaction. Plant Pathol. 45:1139-1149.
38. McFeters, G. A., and LeChevallier, M. W. 2000. Chemical disinfection and injury of bacteria in water. Pages 255-275 in: Nonculturable Microorganisms in the Environment. R. R. Colwell and J. D. Grimes eds. American Society for Microbiology Press, Washington, DC.
39. Minsavage, G. V., Thompson, C. M., Hopkins, D. L., Leite, R. M. V. B. C., and Stall, R. E. 1994. Development of a polymerase chain reaction protocol for detection of Xylella fastidiosa in plant tissue. Phytopathology 84:456-461.
40. Morin, P., Camper, A., Jones, W., Gatel, D., and Goldman, J. C. 1996. Colonization and disinfection of biofilms hosting coliform-colonized carbon fines. Appl. Environ. Microbiol. 62:4428-4432.
41. Norman, D. J., Yuen, J. M. F., and Mangravita-Novo, A. 2006. Susceptibility of geranium cultivars to bacterial wilt. Page 94 in: Abstr. 4th International Bacterial Wilt Symposium. British Society for Plant Pathology, York, UK.
42. Oliver, J. D. 1993. Formation of viable but nonculturable cells. Pages 239-272 in: Starvation in bacteria. S. Kjelleberg, ed. Plenum Press, New York.
43. Oliver, J. D. 2000a. Public health significance of viable but nonculturable bacteria. In: Non-culturable microorganisms in the environment. R. R. Colwell and D. J. Grimes, eds. American Society for Microbiology Press, Washington, DC.
44. Oliver, J. D. 2000b. Problems in detecting dormant (VBNC) cells, and the role of DNA elements in this response. In: Tracking Genetically Engineered Microorganisms. J. K. Jansson, J. D. van Elsas, and M. Bailey, eds. Landes Bioscience, Georgetown, TX.
45. Oliver, J. D., Dagher, M., and Linden, K. 2005. Induction of Escherichia coli and Salmonella typhimurium into the viable but nonculturable state following chlorination of wastewater. J. Water Health 3:249-257.
46. Olmos, A., Bertolini, E., and Cambra, M. 2002. Simultaneous and Cooperational amplification (Co-PCR): A new concept for detection of plant viruses. J. Virol. Methods 106:51-59.
47. Olson, K. 1976. Experiences on brown rot caused by Pseudomonas solanacearum (Smith) Smith in Sweden. Bull. OEPP 6:199-207.
48. Opina, N., Tavner, F., Holloway, G., Wang, J.-F., Li, T. H., Maghirang, R., Fegan, M., Hayward, A. C., Krishnapillai, V., Hong, W. F., Holloway, B. W., and Timmis, J. N. 1997. A novel method for development of species and strain-specific DNA probes and PCR primers for identifying Burkholderia solanacearum (formerly Pseudomonas solanacearum). Asia-Pac. J. Mol. Biol. 5:19-33.
49. Palomo, J. L., Caruso, P., Gorris, M. T., López, M. M., and García-Benavides, P. 2000. Comparación de métodos de detección de Ralstonia solanacearum en aguas superficiales, Page 120 in: Abstr. 10th Congr. Soc. Española Fitopatol., SEF, Madrid, Spain.
50. Pastrik, K. H., and Maiss, E. 2000. Detection of Ralstonia solanacearum in potato tubers by polymerase chain reaction. J. Phytopathol. 148:619-626.
51. Poussier, S., and Luisetti, J. 2000. Specific detection of biovars of Ralstonia solanacearum in plant tissues by nested-PCR-RFLP. Eur. J. Plant Pathol. 106:255-265.
52. Pradhanang, P. M., Elphinstone, J. G., and Fox, R. T. V. 2000. Identification of crop and weed hosts of Ralstonia solanacearum. Plant Pathol. 19:403-413.
53. Seal, S. E., Jakson, L. A., Young, J. P. W., and Daniels, M. J. 1993. Differentiation of Pseudomonas solanacearum, Pseudomonas syzygii, Pseudomonas pickettii, and blood disease bacterium by partial 16S sequencing: Construction of oligonucleotide primers for sensitive detection by polymerase chain reaction. J. Gen. Microbiol. 139:1587-1594.
54. Stewart, M. H., Wolfe, R. L., and Means, E. G. 1990. Assessment of the bacteriological activity associated with granular activated carbon treatment of drinking water. Appl. Environ. Microbiol. 56:3822-3829.
55. Strider, D. L., Jones, R. K., and Haygood, R. A. 1981. Southern bacterial wilt of geranium caused by Pseudomonas solanacearum. Plant Dis. 65:52-53.
56. Sulzinski, M. A., Moorman, G. W., Schlagnhaufer, B., and Romaine, C. P. 1997. A simple DNA extraction method for PCR-based detection of Xanthomonas campestris pv. pelargonii in geraniums. J. Phytopathol. 145:213-215.
57. Swanson, J. K., Yao, J., Tans-Kerstn, J., and Allen, C. 2005. Behavior of Ralstonia solanacearum race 3 biovar 2 during latent and active infection of geranium. Phytopathology 95:136-143.
58. Timms-Wilson, T. M., Bryant, K., and Bailey, M. J. 2001. Strain characterization and 16S-23S probe development for differentiating geographically dispersed isolates of the phytopathogen Ralstonia solanacearum. Environ. Microbiol. 3:785-797.
59. Tusiime, G., Adipala, E., Opio, F., and Bhagsari, A. S. 1998. Weeds as latent hosts of Ralstonia solanacearum in highland Uganda: Implications to development of an integrated control package for bacterial wilt. Pages 413-419 in: Bacterial Wilt Disease: Molecular and Ecological Aspects. P. Prior, C. Allen, and J. Elphinstone, eds. Springer-Verlag, Berlin.
60. van Elsas, J. D., Kastelein, P., de Vries, P. M., van Overbeek, L. S. 2001. Effects of ecological factors on the survival and physiology of Ralstonia solanacearum bv. 2 in irrigation water. Can. J. Microbiol. 47:842-854.
61. van Elsas, J. D., Kastelein, P., van Bekkum, P., van der Wolf, J. M., de Vries, P. M., and van Overbeek, L. S. 2000. Survival of Ralstonia solanacearum biovar 2, the causative agent of potato brown rot, in field and microcosm soils in temperate climates. Phytopathology 90:1358-1366.
62. Weller, S. A., Elphinstone, J. G., Smith, N. C., Boonham, N., and Stead, D. E. 2000. Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay. Appl. Environ. Microbiol. 66:2853-2858.
63. Wenneker, M., Verdel, M. S. W., Groeneveld, R. M. W., Kempenaar, C., van Beuningen, A. R., and Janse, J. D. 1999. Ralstonia (Pseudomonas) solanacearum race 3 (biovar 2) in surface water and natural weed hosts: First report on stinging nettle (Urtica dioica). Eur. J. Plant. Pathol. 105:307-315.
64. Williamson, L., Nakaho, K., Hudelson, B., and Allen, C. 2002. Ralstonia solanacearum race 3, biovar 2 strains isolated from geranium are pathogenic on potato. Plant Dis. 86:987-991.
65. Wood, J. R., Breckenridge, K., and Chard, J. M. 2002. Eradicating Ralstonia solanacearum from Scottish rivers. Page 31 in: Abstr. 3rd Int. Bacterial Wilt Symp. (IBWS), Nelspruit. Tobacco and Cotton Research Institute, Rustenberg, South Africa.
66. Wu, Y., Clevenger, T., and Deng, B. 2005. Impacts of goethite particles on UV disinfection of drinking water. Appl. Environ. Microbiol. 71:4140-4143.
67. Yabuuchi, E., Kosako, Y., Yano, I., Hotta, H., and Nishiuchi, Y. 1995. Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov. Proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov.: Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol. Immunol. 39:897-904.
68. Zhao, L., and Matthews, K. R. 2000. Influence of starvation, temperature, and pH on culturability of Escherichia coli O157:H7. J. Food Safety 20:193-208.