Specific detection of biovars of Ralstonia solanacearum in plant tissues by Nested-PCR-RFLP

European Journal of Plant Pathology

Volumn 106, Issue 3, 2000, Pages 255-265

  Poussier, Stéphane ^a   Luisetti, Jacques ^a  

^a CIRAD   (France)

Author keywords

Bacterial wilt; Biovars discrimination; Diagnosis; hrp

Indexed keywords

AFRICA; BACTERIAL INFECTION; BACTERIAL WILT; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BIOVAR; COLONY FORMING UNIT; GENETIC STRAIN; HRP GENE; OLIGONUCLEOTIDE; PLANT EXTRACT; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

BURKHOLDERIA CEPACIA; PELARGONIUM ASPERUM; PSEUDOMONAS SYZYGII; RALSTONIA PICKETTII; RALSTONIA SOLANACEARUM;

EID: 0034126963     PISSN: 09291873     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008742609761     Document Type: Article

References (55)

1. Arlat M, Gough CL, Zischek C, Barberis PA, Trigalet A and Boucher CA (1992) Transcriptional organization and expression of the large hrp gene cluster of Pseudomonas solanacearum. Mol Plant-Microbe Interact 5: 187-193
2. Audy P, Braat CE, Saindon G, Huang HC and Laroche A (1996) A rapid and sensitive PCR-based assay for concurrent detection of bacteria causing common and halo blights in bean seed. Phytopathology 86: 361-366
3. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA and Struhl K (1991) Current Protocols in Molecular Biology. Greene Publishing Associates - Wiley Interscience, New York, USA
4. Boucher CA, Gough CL and Arlat M (1992) Molecular genetics of pathogenicity determinants of Pseudomonas solanacearum with special emphasis on hrp genes. Annu Rev Phytopathol 30: 443-461
5. Buddenhagen I, Sequeira L and Kelman A (1962) Designation of races in Pseudomonas solanacearum. Phytopathology 52: 726
6. Buddenhagen IW (1986) Bacterial wilt revisited. In: Persley GJ (ed) Bacterial Wilt Disease in Asia and the South Pacific. Proc Int Workshop (pp 126-143) Los Banos, The Philippines. ACIAR Proc. 13, Canberra, Australia
7. Ciampi L, Sequeira L and French ER (1980) Latent infection of potato tubers by Pseudomonas solanacearum. Am Potato J 57:377-386
8. Cook D, Barlow E and Sequeira L (1989) Genetic diversity of Pseudomonas solanacearum: detection of restriction fragment length polymorphisms with DNA probes that specify virulence and the hypersensitive response. Mol Plant-Microbe Interact 2: 113-121
9. Cook D, Barlow E and Sequeira L (1991) DNA probes as tools for the study of host-pathogen evolution: the example of Pseudomonas solanacearum. In: Henneke H and Verma DPS (eds) Advances in Molecular Genetics of Plant-Microbe Interactions. Vol I (pp 103-108) Kluwer Academics Publishers, Dordrecht, The Netherlands
10. Cook D and Sequeira L (1994) Strain differentiation of Pseudomonas solanacearum by molecular genetics methods. In: Hayward AC and Hartman GL (eds) Bacterial Wilt: The Disease and its Causative Agent, Pseudomonas solanacearum (pp 77-93) CAB International, Wallingford, United Kingdom
11. Demeke T and Adams RP (1992) The effects of plant polysaccharides and buffer additives on PCR. BioTechniques 12: 332-334
12. Eden-Green SJ (1994) Diversity of Pseudomonas solanacearum and related bacteria in South East Asia: new direction for moko disease. In: Hayward AC and Hartman GL (eds) Bacterial Wilt: The Disease and its Causative Agent, Pseudomonas solanacearum (pp 25-34) CAB International, Wallingford, United Kingdom
13. Eden-Green SJ and Sastraatmadja H (1990) Blood disease present in Java. FAO Plant Prot Bull 38: 49-50
14. Girard JC, Nicole JF, Chéron JJ, Gaubiac AM, Huvier O, Oudard B and Suzor H (1993) Bacterial wilt due to Pseudomonas solanacearum in Reunion: general situation and current research. In: Hartman GL and Hayward AC (eds) Bacterial Wilt Proc Int Conf (pp 343-347) Kaohsiung, Taïwan. ACIAR Proc. 45, Canberra, Australia
15. Granada GA and Sequeira L (1983) A new selective medium for Pseudomonas solanacearum. Plant Dis 67: 1084-1088
16. Hartung JS, Pruvost OP, Villemot I and Alvarez A (1996) Rapid and sensitive colorimetric detection of Xanthomonas axonopodis pv. citri by immunocapture and a nested-polymerase chain reaction assay. Phytopathology 86: 95-101
17. Hayward AC (1964) Characteristics of Pseudomonas solanacearum. J Appl Bacteriol 27: 265-277
18. Hayward AC (1991) Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu Rev Phytopathol 29: 65-87
19. Hayward AC, El-Nashaar HM, Nydegger U and De Lindo L (1990) Variation in nitrate metabolism in biovars of Pseudomonas solanacearum. J Appl Bacteriol 69: 269-280
20. He LY, Sequeira L and Kelman A (1983) Characteristics of strains of Pseudomonas solanacearum. Plant Dis 67: 1357-1361
21. Hu X, Lai F-M, Reddy SN and Ishimaru CA (1995) Quantitative detection of Clavibacter michiganensis subsp. sepedonicus by competitive polymerase chain reaction. Phytopathology 85: 1468-1473
22. Kelman A (1954) The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium. Phytopathology 44: 693-695
23. Kersters K, Ludwig W, Vancanneyt M, De Vos P, Gillis M and Schleifer K-H (1996) Recent changes in the classification of the Pseudomonads: an overview. Syst Appl Microbiol 19: 465-477
24. Kirchhof G, Baldani JI, Reis VM and Hartmann A (1998) Molecular assay to identify Acetobacter diazotrophicus and detect its occurence in plant tissues. Can J Microbiol 44: 12-19
25. Kreader CA (1996) Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Appl Environ Microbiol 62: 1102-1106
26. Leite RPJ, Egel DS and Stall RE (1994a) Genetic analysis of hrp-related DNA sequences of Xanthomonas campestris strains causing diseases of citrus. Appl Environ Microbiol 60: 1078-1086
27. Leite RPJ, Minsavage GV, Bonas U and Stall RE (1994b) Detection and identification of phytopathogenic Xanthomonas strains by amplification of DNA sequences related to the hrp genes of Xanthomonas campestris pv. vesicatoria. Appl Environ Microbiol 60: 1068-1077
28. Leite RP Jr, Jones JB, Somodi GC, Minsavage GV and Stall RE (1995) Detection of Xanthomonas campestris pv. vesicatoria associated with pepper and tomato seed by DNA amplification. Plant Dis 79: 917-922
29. Li X and De Boer SH (1995) Selection of polymerase chain reaction primers from an RNA intergenic spacer region for specific detection of Clavibacter michiganensis subsp. sepedonicus. Phytopathology 85: 837-842
30. Li X, Dorsch M, Del Dot T, Sly LI, Stackebrandt E and Hayward AC (1993) Phylogenetic studies of the rRNA group II pseudomonads based on 16S rRNA gene sequences. J Appl Bacteriol 74: 324-329
31. Macs M, Garbeva P and Crepel C (1996) Identification and sensitive endophytic detection of the fire blight pathogen Erwinia amylovora with 23S ribosomal DNA sequences and the polymerase chain reaction. Plant Pathol 45: 1139-1149
32. McGregor DP, Forster S, Steven J, Adair J, Leary SEC, Leslie DL, Harris WJ and Titball RW (1996) Simultaneous detection of microorganisms in soil suspension based on PCR amplification of bacterial 16S rRNA. BioTechniques 21: 463-471
33. McManus PS and Jones AL (1995) Detection of Erwinia amylovora by nested PCR and PCR-dot-blot and reverse-blot hybridizations. Phytopathology 85: 618-623
34. Minsavage GV, Thompson CM, Hopkins DL, Leite RMVBC and Stall RE (1994) Development of a polymerase chain reaction protocol for detection of Xylella fastidiosa in plant tissue. Phytopathology 84: 456-461
35. National Biosciences I (1996) Oligo, primer analysis software, version 5.0. Plymouth, MN, USA
36. Nicole JF (1995) Influence de facteurs biotique ou abiotique sur la dynamique des populations de Pseudomonas solanacearum au cours de l'infection et sur le développement de la maladie. Ph.D. thesis, Université de Nantes, France
37. Nyangeri JB, Gathuru EM and Mukuya DM (1984) Effect of latent infection on the spread of bacterial wilt of potatoes in Kenya. Tropical Pest Management 30: 163-165
38. Palleroni NJ (1984) Genus I. Pseudomonas. In: Krieg NR and Holt JG (eds) Bergey's Manual of Systematic Bacteriology. Vol I (pp 141-199) Williams & Wilkins, Baltimore, USA
39. Pegg K and Moffett M (1971) Host range of the ginger strain of Pseudomonas solanacearum in Queensland. Aust J Exp Agric Anim Husb 11: 696-698
40. Picard C, Ponsonnet C, Paget E, Nesme X and Simonet P (1992) Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction. Appl Environ Microbiol 58: 2717-2722
41. Poussier S, Vandewalle P and Luisetti J (1999) Genetic diversity of African and worldwide strains of Ralstonia solanacearum as determined by PCR-Restriction Fragment Length Polymorphism analysis of the hrp gene region. Appl Environ Microbiol 65: 2184-2194
42. Prior P, Béramis M, Chillet M and Schmit J (1990) Preliminary studies for tomato bacterial wilt (Pseudomonas solanacearum E.F.Sm.) resistance mechanisms. Symbiosis 9: 393-400
43. Prior P and Steva H (1990) Characteristics of strains of Pseudomonas solanacearum from the French West Indies. Plant Dis 74: 13-17
44. Roberts PD, Jones JB, Chandler CK, Stall RE and Berger RD (1996) Survival of Xanthomonas fragariae on strawberry in summer nurseries in Florida detected by specific primers and nested polymerase chain reaction. Plant Dis 80: 1283-1288
45. Roberts SJ, Eden-Green SJ, Jones P and Ambler DJ (1990) Pseudomonas syzygii sp. nov., the cause of Sumatra disease of cloves. Syst Appl Microbiol 13: 34-43
46. Seal SE, Jackson LA and Daniels MJ (1992a) Use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification. Appl Environ Microbiol 58: 3759-3761
47. Seal SE, Jackson LA and Daniels MJ (1992b) Isolation of Pseudomonas solanacearum-specific DNA probe by subtraction hybridization and construction of species-specific oligonucleotide primers for sensitive detection by the polymerase chain reaction. Appl Environ Microbiol 58: 3751-3758
48. Seal SE, Jackson LA, Young JPW and Daniels MJ (1993) Differentiation of Pseudomonas solanacearum, Pseudomonas syzygii, Pseudomonas pickettii and the Blood Disease Bacterium by partial 16S rRNA sequencing: construction of oligonucleotide primers for sensitive detection by polymerase chain reaction. J Gen Microbiol 139: 1587-1594
49. Seal SE, Taghavi M, Fegan N, Hayward AC and Fegan M (1999) Determination of Ralstonia (Pseudomonas) solanacearum rDNA subgroups by PCR tests. Plant Pathol 48: 115-120
50. Stead DE, Elphinstone JG and Pemberton AW (1996) Potato brown rot in Europe. Brighton Crop Protection Conference -Pests and Diseases (pp 1145-1152)
51. Steffan RJ and Atlas RM (1988) DNA amplification to enhance detection of genetically engineered bacteria in environmental samples. Appl Environ Microbiol 54: 2185-2191
52. Taghavi M, Hayward C, Sly LI and Fegan M (1996) Analysis of the phylogenetic relationships of strains of Burkholderia solanacearum, Pseudomonas syzygii, and the Blood Disease Bacterium of banana based on 16S rRNA gene sequences. Int J Syst Bacteriol 46: 10-15
53. Tsai Y-L and Olson BH (1992) Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction. Appl Environ Microbiol 58: 754-757
54. Wilson IG (1997) Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63: 3741-3751
55. Young CC, Burghoff RL, Keim LG, Minak-Bernero V, Lute JR and Hinton SM (1993) Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils. Appl Environ Microbiol 59: 1972-1974