Fusion core structure of the severe acute respiratory syndrome coronavirus (SARS-CoV): In search of potent SARS-CoV entry inhibitors

Journal of Cellular Biochemistry

Volumn 104, Issue 6, 2008, Pages 2335-2347

  Chu, Ling Hon Matthew ^a   Chan, Siu Hong ^a   Tsai, Sau Na ^a   Wang, Yi ^b   Cheng, Christopher Hon Ki ^a   Wong, Kam Bo ^a   Waye, Mary Miu Yee ^a   Ngai, Sai Ming ^a  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

^b PEKING UNIVERSITY   (China)

Author keywords

Fusion core; Peptide; SARS CoV

Indexed keywords

ADS J1; ANTIVIRUS AGENT; HEPTAD REPEAT PROTEIN; HKC 21; HKC 24; HKC 25; HKC 5; HR1 A PROTEIN; HR1 B PROTEIN; HR1 PROTEIN; HR2 PROTEIN; QUERCETIN; RECOMBINANT PROTEIN; SYNTHETIC PEPTIDE; VIRUS FUSION PROTEIN; VIRUS PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BIOASSAY; BIOLOGICAL ACTIVITY; CD4+ T LYMPHOCYTE; CONTROLLED STUDY; DRUG ACTIVITY; DRUG MECHANISM; DRUG TARGETING; HUMAN IMMUNODEFICIENCY VIRUS 1; HYDROPHOBICITY; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSEUDOTYPING; SARS CORONAVIRUS; VIRUS ENTRY; VIRUS INHIBITION;

CORONAVIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; SARS CORONAVIRUS;

EID: 49049103345     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.21790     Document Type: Article

References (23)

1. Chim SS, Tsui SK, Chan KC, Au TC, Hung EC, Tong YK, Chiu RW, Ng EK, Chan PK, Chu CM, Sung JJ, Tam JS, Fung KP, Waye MM, Lee CY, Yuen KY, Lo YM. 2003. Genomic characterisation of the severe acute respiratory syndrome coronavirus of Amoy Gardens outbreak in Hong Kong. Lancet 362:1807-1808.
2. Choy WY, Lin SG, Chan PK, Tam JS, Lo YM, Chu IM, Tsai SN, Zhong MQ, Fung KP, Waye MM, Tsui SK, Ng KO, Shan ZX, Yang M, Wu YL, Lin ZY, Ngai SM. 2004. Synthetic peptide studies on the severe acute respiratory syndrome (SARS) coronavirus spike glycoprotein: Perspective for SARS vaccine development. Clin Chem 50:1036-1042.
3. Chu LH, Choy WY, Tsai SN, Rao Z, Ngai SM. 2006. Rapid peptide-based screening on the substrate specificity of severe acute respiratory syndrome (SARS) coronavirus 3C-like protease by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Protein Sci 15:699-709.
4. Connor RI, Chen BK, Choe S, Landau NR. 1995. Vpr is required for efficient replication of human immuno-deficiency virus type-1 in mononuclear phagocytes. Virology 206:935-944.
5. Deng Y, Liu J, Zheng Q, Yong W, Lu M. 2006. Structures and polymorphic interactions of two heptad-repeat regions of the SARS virus S2 protein. Structure 14:889-899.
6. Duquerroy S, Vigouroux A, Rottier PJ, Rey FA, Bosch BJ. 2005. Central ions and lateral asparagine/glutamine zippers stabilize the post-fusion hairpin conformation of the SARS Coronavirus spike glycoprotein. Virology 335:276-285.
7. Eckert DM, Kim PS. 2001a. Design of potent inhibitors of HIV-1 entry from the gp41 N-peptide region. Proc Natl Acad Sci USA 98:11187-11192.
8. Eckert DM, Kim PS. 2001b. Mechanisms of viral membrane fusion and its inhibition. Annu Rev Biochem 70:777-810.
9. Hofmann H, Pohlmann S. 2004. Cellular entry of the SARS Coronavirus. Trends Microbiol 12:466-472.
10. Kilby JM, Hopkins S, Venetta TM, DiMassimo B, Cloud GA, Lee JY, Alldredge L, Hunter E, Lambert D, Bolognesi D, Matthews T, Johnson MR, Nowak MA, Shaw GM, Saag MS. 1998. Potent suppression of HIV-1 replication in humans by T-20, a peptide inhibitor of gp41-mediated virus entry. Nat Med 4:1302-1307.
11. Kohn WD, Kay CM, Hodges RS. 1998. Orientation, positional, additivity, and oligomerization-state effects of interhelical ion pairs in alpha-helical coiled-coils. J Mol Biol 283:993-1012.
12. Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, Tong S, Urbani C, Comer JA, Lim W, Rollin PE, Dowell SF, Ling AE, Humphrey CD, Shieh WJ, Guarner J, Paddock CD, Rota P, Fields B, DeRisi J, Yang JY, Cox N, Hughes JM, LeDuc JW, Bellini WJ, Anderson LJ. 2003. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med 348:1953-1966.
13. Lee N, Hui D, Wu A, Chan P, Cameron P, Joynt GM, Ahuja A, Yung MY, Leung CB, To KF, Lui SF, Szeto CC, Chung S, Sung JJ. 2003. A major outbreak of severe acute respiratory syndrome in Hong Kong. N Engl J Med 348:1986-1994.
14. Lio P, Goldman N. 2004. Phylogenomics and bioinformatics of SARS-CoV. Trends Microbiol 12:106-111.
15. Liu S, Xiao G, Chen Y, He Y, Niu J, Escalante CR, Xiong H, Farmar J, Debnath AK, Tien P, Jiang S. 2004. Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: Implications for virus fusogenic mechanism and identification of fusion inhibitors. Lancet 363:938-947.
16. Nie Y, Wang P, Shi X, Wang G, Chen J, Zheng A, Wang W, Wang Z, Qu X, Luo M, Tan L, Song X, Yin X, Chen J, Ding M, Deng H. 2004. Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression. Biochem Biophys Res Commun 321:994-1000.
17. Spiga O, Bernini A, Ciutti A, Chiellini S, Menciassi N, Finetti F, Causarono V, Anselmi F, Prischi F, Niccolai N. 2003. Molecular modelling of S1 and S2 subunits of SARS Coronavirus spike glycoprotein. Biochem Biophys Res Commun 310:78-83.
18. Supekar VM, Bruckmann C, Ingallinella P, Bianchi E, Pessi A, Carfi A. 2004. Structure of a proteolytically resistant core from the severe acute respiratory syndrome coronavirus S2 fusion protein. Proc Natl Acad Sci USA 101:17958-17963.
19. Tripet B, Howard MW, Jobling M, Holmes RK, Holmes KV, Hodges RS. 2004. Structural characterization of the SARS-coronavirus spike S fusion protein core. J Biol Chem 279:20836-20849.
20. Wolf E, Kim PS, Berger B. 1997. MultiCoil: A program for predicting two- and three-stranded coiled coils. Protein Sci 6:1179-1189.
21. Xu Y, Lou Z, Liu Y, Pang H, Tien P, Gao GF, Rao Z. 2004a. Crystal structure of severe acute respiratory syndrome Coronavirus spike protein fusion core. J Biol Chem 279:49414-49419.
22. Xu Y, Zhu J, Liu Y, Lou Z, Yuan F, Liu Y, Cole DK, Ni L, Su N, Qin L, Li X, Bai Z, Bell JI, Pang H, Tien P, Gao GF, Rao Z. 2004b. Characterization of the heptad repeat regions, HR1 and HR2, and design of a fusion core structure model of the spike protein from severe acute respiratory syndrome (SARS) coronavirus. Biochemistry 43:14064-14071.
23. Zhu J, Xiao G, Xu Y, Yuan F, Zheng C, Liu Y, Yan H, Cole DK, Bell JI, Rao Z, Tien P, Gao GF. 2004. Following the rule: Formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors. Biochem Biophys Res Commun 319:283-288.